Restriction map for
pF1KB6824
(Circular) (Six-base) MAPSORT of: pF1KB6824.seq Check: 2929 from: 1 to: 3674
pF1KB6824 3674 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 3514 3514
Size: 3674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1499 1931 2678 2946 1499
Size: 432 747 268 2227
Fragments arranged by size:
2227 747 432 268
AflIII A'CryG_T
Cuts at: 386 1026 2130 3107 386
Size: 640 1104 977 953
Fragments arranged by size:
1104 977 953 640
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 979 2546 979
Size: 1567 2107
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 678 3027 3283 678
Size: 2349 256 1069
Fragments arranged by size:
2349 1069 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 416 694 416
Size: 278 3396
BamHI G'GATC_C
Cuts at: 699 699
Size: 3674
BanI G'GyrC_C
Cuts at: 690 1104 1352 1387 690
Size: 414 248 35 2977
Fragments arranged by size:
2977 414 248 35
BanII G_rGCy'C
Cuts at: 256 302 425 688 1718 256
Size: 46 123 263 1030 2212
Fragments arranged by size:
2212 1030 263 123 46
BbeI G_GCGC'C
Cuts at: 1108 1356 1108
Size: 248 3426
BbvCI CC'TCA_GC
Cuts at: 646 646
Size: 3674
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 1194 1194
Size: 3674
BglII A'GATC_T
Cuts at: 446 1189 3226 446
Size: 743 2037 894
Fragments arranged by size:
2037 894 743
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 272 1299 1392 2448 272
Size: 1027 93 1056 1498
Fragments arranged by size:
1498 1056 1027 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 967 1302 3446 886
Size: 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3674
Bpu10I CC'TnA_GC
Cuts at: 646 646
Size: 3674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 409 850 1729 2221 2519 175
Size: 234 441 879 492 298 1330
Fragments arranged by size:
1330 879 492 441 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 3378 3378
Size: 3674
BsaAI yAC'GTr
Cuts at: 267 1027 267
Size: 760 2914
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 1105 1353 2055 3551 1105
Size: 248 702 1496 1228
Fragments arranged by size:
1496 1228 702 248
BsaWI w'CCGG_w
Cuts at: 662 863 1384 2062 2336 2483 2894 662
Size: 201 521 678 274 147 411 1442
Fragments arranged by size:
1442 678 521 411 274 201 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 550 583 547
Size: 3 33 3638
Fragments arranged by size:
3638 33 3
BseYI C'CCAG_C
Cuts at: 597 1099 1857 2434 2930 597
Size: 502 758 577 496 1341
Fragments arranged by size:
1341 758 577 502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 147 147
Size: 3674
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 302 401 425 688 1466 1656 2448 302
Size: 99 24 263 778 190 792 1528
Fragments arranged by size:
1528 792 778 263 190 99 24
BsmI GAATG_Cn'
Cuts at: 198 198
Size: 3674
Bsp1286I G_dGCh'C
Cuts at: 256 272 302 401 425 688 1299 1392 1466
Size: 16 30 99 24 263 611 93 74
Cuts at: 1466 1656 1718 2448 256
Size: 190 62 730 1482
Fragments arranged by size:
1482 730 611 263 190 99 93 74
62 30 24 16
BspEI T'CCGG_A
Cuts at: 662 2894 662
Size: 2232 1442
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 724 938 1240 1621 724
Size: 214 302 381 2777
Fragments arranged by size:
2777 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1966 2020 3504 3669 50
Size: 1916 54 1484 165 55
Fragments arranged by size:
1916 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 1586
Size: 3674
BsrFI r'CCGG_y
Cuts at: 1672 1853 2062 1672
Size: 181 209 3284
Fragments arranged by size:
3284 209 181
BssSI C'ACGA_G
Cuts at: 1945 2303 1945
Size: 358 3316
BstAPI GCAn_nnn'nTGC
Cuts at: 436 1051 436
Size: 615 3059
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstYI r'GATC_y
Cuts at: 1 446 699 1166 1189 1524 1770 2782 2868
Size: 445 253 467 23 335 246 1012 86
Cuts at: 2868 2880 3226 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 253 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3674
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 917 917
Size: 3674
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 1380 2238 1380
Size: 858 2816
EaeI y'GGCC_r
Cuts at: 1259 1433 1824 1851 3196 1259
Size: 174 391 27 1345 1737
Fragments arranged by size:
1737 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 131 244 1697 1907 131
Size: 113 1453 210 1898
Fragments arranged by size:
1898 1453 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 300 423 686 300
Size: 123 263 3288
Fragments arranged by size:
3288 263 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1499 1931 2678 2946 511
Size: 988 432 747 268 1239
Fragments arranged by size:
1239 988 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 486 633 813 486
Size: 147 180 3347
Fragments arranged by size:
3347 180 147
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 618 1693 618
Size: 1075 2599
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 1108 1356 2378 3357 1108
Size: 248 1022 979 1425
Fragments arranged by size:
1425 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1516 1546 1548 1578 1516
Size: 30 2 30 3612
Fragments arranged by size:
3612 30 30 2
HincII GTy'rAC
Cuts at: 713 713
Size: 3674
KasI G'GCGC_C
Cuts at: 1104 1352 1104
Size: 248 3426
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2529 2345
Size: 184 3490
MscI TGG'CCA
Cuts at: 1435 1435
Size: 3674
MslI CAynn'nnrTG
Cuts at: 289 1663 1790 289
Size: 1374 127 2173
Fragments arranged by size:
2173 1374 127
MspA1I CmG'CkG
Cuts at: 785 1099 1459 2472 2717 785
Size: 314 360 1013 245 1742
Fragments arranged by size:
1742 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1855 1855
Size: 3674
NarI GG'CG_CC
Cuts at: 1105 1353 1105
Size: 248 3426
NcoI C'CATG_G
Cuts at: 107 107
Size: 3674
NgoMIV G'CCGG_C
Cuts at: 1853 1853
Size: 3674
NruI TCG'CGA
Cuts at: 204 204
Size: 3674
NspI r_CATG'y
Cuts at: 390 727 1227 2134 390
Size: 337 500 907 1930
Fragments arranged by size:
1930 907 500 337
PciI A'CATG_T
Cuts at: 386 2130 386
Size: 1744 1930
PfoI T'CCnGG_A
Cuts at: 302 302
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PmlI CAC'GTG
Cuts at: 267 267
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PpuMI rG'GwC_Cy
Cuts at: 486 486
Size: 3674
PstI C_TGCA'G
Cuts at: 721 1406 721
Size: 685 2989
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 1099 1459 1099
Size: 360 3314
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 302 425 688 302
Size: 123 263 3288
Fragments arranged by size:
3288 263 123
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 131 244 1697 1907 131
Size: 113 1453 210 1898
Fragments arranged by size:
1898 1453 210 113
SbfI CC_TGCA'GG
Cuts at: 721 721
Size: 3674
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 243 717 1402 2395 2586 33
Size: 210 474 685 993 191 1121
Fragments arranged by size:
1121 993 685 474 210 191
SfoI GGC'GCC
Cuts at: 1106 1354 1106
Size: 248 3426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 696 696
Size: 3674
SmlI C'TyrA_G
Cuts at: 190 424 829 1744 2236 2498 190
Size: 234 405 915 492 262 1366
Fragments arranged by size:
1366 915 492 405 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StyI C'CwwG_G
Cuts at: 107 629 808 1162 107
Size: 522 179 354 2619
Fragments arranged by size:
2619 522 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1257 1390 1849 2058 1257
Size: 133 459 209 2873
Fragments arranged by size:
2873 459 209 133
TatI w'GTAC_w
Cuts at: 3189 3189
Size: 3674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 3642
Tth111I GACn'n_nGTC
Cuts at: 559 1471 559
Size: 912 2762
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1169 1169
Size: 3674
XmaI C'CCGG_G
Cuts at: 694 694
Size: 3674
XmnI GAAnn'nnTTC
Cuts at: 313 313
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI