Restriction map for
pF1KB6829
(Circular) (Six-base) MAPSORT of: pF1KB6829.seq Check: 6483 from: 1 to: 3611
pF1KB6829 3611 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 605 3451 605
Size: 2846 765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1868 2615 2883 1436
Size: 432 747 268 2164
Fragments arranged by size:
2164 747 432 268
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 309 615 2964 3220 309
Size: 306 2349 256 700
Fragments arranged by size:
2349 700 306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 631 631
Size: 3611
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 223 349 627 1041 1289 1324 223
Size: 126 278 414 248 35 2510
Fragments arranged by size:
2510 414 278 248 126 35
BanII G_rGCy'C
Cuts at: 492 625 1655 492
Size: 133 1030 2448
Fragments arranged by size:
2448 1030 133
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BbsI GAAGACnn'nnnn_
Cuts at: 269 578 269
Size: 309 3302
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 1131 1131
Size: 3611
BglI GCCn_nnn'nGGC
Cuts at: 221 358 221
Size: 137 3474
BglII A'GATC_T
Cuts at: 288 1126 3163 288
Size: 838 2037 736
Fragments arranged by size:
2037 838 736
BlpI GC'TnA_GC
Cuts at: 723 723
Size: 3611
Bme1580I G_kGCm'C
Cuts at: 1236 1329 2385 1236
Size: 93 1056 2462
Fragments arranged by size:
2462 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 465
Size: 3611
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 787 1666 2158 2456 516
Size: 271 879 492 298 1671
Fragments arranged by size:
1671 879 492 298 271
BsaI GGTCTCn'nnnn_
Cuts at: 265 468 3315 265
Size: 203 2847 561
Fragments arranged by size:
2847 561 203
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 287 1125 3162 6
Size: 281 838 2037 455
Fragments arranged by size:
2037 838 455 281
BsaHI Gr'CG_yC
Cuts at: 1042 1290 1992 3488 1042
Size: 248 702 1496 1165
Fragments arranged by size:
1496 1165 702 248
BsaWI w'CCGG_w
Cuts at: 800 1321 1999 2273 2420 2831 800
Size: 521 678 274 147 411 1580
Fragments arranged by size:
1580 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 565
Size: 30 3581
BseYI C'CCAG_C
Cuts at: 1036 1794 2371 2867 1036
Size: 758 577 496 1780
Fragments arranged by size:
1780 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 335 625 1403 1593 2385 335
Size: 290 778 190 792 1561
Fragments arranged by size:
1561 792 778 290 190
Bsp1286I G_dGCh'C
Cuts at: 335 492 625 1236 1329 1403 1593 1655 2385
Size: 157 133 611 93 74 190 62 730
Cuts at: 2385 335
Size: 1561
Fragments arranged by size:
1561 730 611 190 157 133 93 74
62
BspEI T'CCGG_A
Cuts at: 2831 2831
Size: 3611
BspHI T'CATG_A
Cuts at: 2787 2787
Size: 3611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 875 1177 1558 661
Size: 214 302 381 2714
Fragments arranged by size:
2714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 1609 1790 1999 1609
Size: 181 209 3221
Fragments arranged by size:
3221 209 181
BssSI C'ACGA_G
Cuts at: 1882 2240 1882
Size: 358 3253
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstYI r'GATC_y
Cuts at: 1 288 636 1103 1126 1461 1707 2719 2805
Size: 287 348 467 23 335 246 1012 86
Cuts at: 2805 2817 3163 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 348 346 335 287 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 1742 1905 1561
Size: 181 163 3267
Fragments arranged by size:
3267 181 163
BtsI GCAGTG_nn'
Cuts at: 456 504 854 456
Size: 48 350 3213
Fragments arranged by size:
3213 350 48
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 397 1317 2175 397
Size: 920 858 1833
Fragments arranged by size:
1833 920 858
EaeI y'GGCC_r
Cuts at: 1196 1370 1761 1788 3133 1196
Size: 174 391 27 1345 1674
Fragments arranged by size:
1674 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1819 2129 2275 3421 1819
Size: 310 146 1146 2009
Fragments arranged by size:
2009 1146 310 146
EcoICRI GAG'CTC
Cuts at: 623 623
Size: 3611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 1436 1868 2615 2883 465
Size: 971 432 747 268 1193
Fragments arranged by size:
1193 971 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 455 750 455
Size: 295 3316
EcoRI G'AATT_C
Cuts at: 309 615 309
Size: 306 3305
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 600 1630 600
Size: 1030 2581
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 1045 1293 2315 3294 1045
Size: 248 1022 979 1362
Fragments arranged by size:
1362 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 1453 1483 1485 1515 565
Size: 32 856 30 2 30 2661
Fragments arranged by size:
2661 856 32 30 30 2
HincII GTy'rAC
Cuts at: 650 650
Size: 3611
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2466 2282
Size: 184 3427
MscI TGG'CCA
Cuts at: 1372 1372
Size: 3611
MslI CAynn'nnrTG
Cuts at: 1600 1727 1600
Size: 127 3484
MspA1I CmG'CkG
Cuts at: 212 437 722 1036 1396 2409 2654 212
Size: 225 285 314 360 1013 245 1169
Fragments arranged by size:
1169 1013 360 314 285 245 225
NaeI GCC'GGC
Cuts at: 1792 1792
Size: 3611
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NcoI C'CATG_G
Cuts at: 107 107
Size: 3611
NgoMIV G'CCGG_C
Cuts at: 1790 1790
Size: 3611
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PpuMI rG'GwC_Cy
Cuts at: 455 455
Size: 3611
PstI C_TGCA'G
Cuts at: 658 1343 658
Size: 685 2926
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 212 1036 1396 212
Size: 824 360 2427
Fragments arranged by size:
2427 824 360
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 625 625
Size: 3611
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SanDI GG'GwC_CC
Cuts at: 455 455
Size: 3611
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 658 658
Size: 3611
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SexAI A'CCwGG_T
Cuts at: 320 320
Size: 3611
SfcI C'TryA_G
Cuts at: 33 654 1339 2332 2523 33
Size: 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 191
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 633 633
Size: 3611
SmlI C'TyrA_G
Cuts at: 531 766 1681 2173 2435 531
Size: 235 915 492 262 1707
Fragments arranged by size:
1707 915 492 262 235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StyI C'CwwG_G
Cuts at: 107 168 745 1099 107
Size: 61 577 354 2619
Fragments arranged by size:
2619 577 354 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1194 1327 1786 1995 352
Size: 842 133 459 209 1968
Fragments arranged by size:
1968 842 459 209 133
TatI w'GTAC_w
Cuts at: 3126 3126
Size: 3611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3579
Tth111I GACn'n_nGTC
Cuts at: 1408 1408
Size: 3611
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 1106
Size: 3611
XmaI C'CCGG_G
Cuts at: 631 631
Size: 3611
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI