Restriction map for
pF1KB6831
(Circular) (Six-base) MAPSORT of: pF1KB6831.seq Check: 5028 from: 1 to: 3611
pF1KB6831 3611 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 649 649
Size: 3611
Acc65I G'GTAC_C
Cuts at: 627 627
Size: 3611
AclI AA'CG_TT
Cuts at: 3451 3451
Size: 3611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1868 2615 2883 1436
Size: 432 747 268 2164
Fragments arranged by size:
2164 747 432 268
AflIII A'CryG_T
Cuts at: 963 2067 3044 963
Size: 1104 977 1530
Fragments arranged by size:
1530 1104 977
AgeI A'CCGG_T
Cuts at: 1999 1999
Size: 3611
AleI CACnn'nnGTG
Cuts at: 142 142
Size: 3611
AlwNI CAG_nnn'CTG
Cuts at: 916 2483 916
Size: 1567 2044
ApaLI G'TGCA_C
Cuts at: 2381 2381
Size: 3611
ApoI r'AATT_y
Cuts at: 615 2964 3220 615
Size: 2349 256 1006
Fragments arranged by size:
2349 1006 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3611
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
AvaI C'yCGr_G
Cuts at: 180 211 631 180
Size: 31 420 3160
Fragments arranged by size:
3160 420 31
BamHI G'GATC_C
Cuts at: 636 636
Size: 3611
BanI G'GyrC_C
Cuts at: 489 627 1041 1289 1324 489
Size: 138 414 248 35 2776
Fragments arranged by size:
2776 414 248 138 35
BanII G_rGCy'C
Cuts at: 575 625 1655 575
Size: 50 1030 2531
Fragments arranged by size:
2531 1030 50
BbeI G_GCGC'C
Cuts at: 1045 1293 1045
Size: 248 3363
BbsI GAAGACnn'nnnn_
Cuts at: 261 261
Size: 3611
BciVI GTATCCnnnnn_n'
Cuts at: 1509 2270 3611 1509
Size: 761 1341 1509
Fragments arranged by size:
1509 1341 761
BclI T'GATC_A
Cuts at: 398 1131 398
Size: 733 2878
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3611
BglII A'GATC_T
Cuts at: 1126 3163 1126
Size: 2037 1574
BlpI GC'TnA_GC
Cuts at: 723 723
Size: 3611
Bme1580I G_kGCm'C
Cuts at: 494 1236 1329 2385 494
Size: 742 93 1056 1720
Fragments arranged by size:
1720 1056 742 93
BmrI ACTGGGnnnn_n'
Cuts at: 823 904 1239 3383 823
Size: 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 206 1978 206
Size: 1772 1839
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 787 1666 2158 2456 787
Size: 879 492 298 1942
Fragments arranged by size:
1942 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 222 523 3315 222
Size: 301 2792 518
Fragments arranged by size:
2792 518 301
BsaAI yAC'GTr
Cuts at: 964 964
Size: 3611
BsaBI GATnn'nnATC
Cuts at: 6 1125 3162 6
Size: 1119 2037 455
Fragments arranged by size:
2037 1119 455
BsaHI Gr'CG_yC
Cuts at: 186 1042 1290 1992 3488 186
Size: 856 248 702 1496 309
Fragments arranged by size:
1496 856 702 309 248
BsaWI w'CCGG_w
Cuts at: 120 800 1321 1999 2273 2420 2831 120
Size: 680 521 678 274 147 411 900
Fragments arranged by size:
900 680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 176 206 176
Size: 30 3581
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 316 349 352 361 184
Size: 132 33 3 9 3434
Fragments arranged by size:
3434 132 33 9 3
BseYI C'CCAG_C
Cuts at: 231 410 1036 1794 2371 2867 231
Size: 179 626 758 577 496 975
Fragments arranged by size:
975 758 626 577 496 179
BsiEI CG_ry'CG
Cuts at: 104 1153 1199 2407 104
Size: 1049 46 1208 1308
Fragments arranged by size:
1308 1208 1049 46
BsiHKAI G_wGCw'C
Cuts at: 575 625 1403 1593 2385 575
Size: 50 778 190 792 1801
Fragments arranged by size:
1801 792 778 190 50
BsmBI CGTCTCn'nnnn_
Cuts at: 177 177
Size: 3611
Bsp1286I G_dGCh'C
Cuts at: 494 575 625 1236 1329 1403 1593 1655 2385
Size: 81 50 611 93 74 190 62 730
Cuts at: 2385 494
Size: 1720
Fragments arranged by size:
1720 730 611 190 93 81 74 62
50
BspEI T'CCGG_A
Cuts at: 120 2831 120
Size: 2711 900
BspHI T'CATG_A
Cuts at: 563 2787 563
Size: 2224 1387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 875 1177 1558 661
Size: 214 302 381 2714
Fragments arranged by size:
2714 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1903 1957 3441 3606 50
Size: 1853 54 1484 165 55
Fragments arranged by size:
1853 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1523 1523
Size: 3611
BsrFI r'CCGG_y
Cuts at: 1609 1790 1999 1609
Size: 181 209 3221
Fragments arranged by size:
3221 209 181
BssSI C'ACGA_G
Cuts at: 1882 2240 1882
Size: 358 3253
BstAPI GCAn_nnn'nTGC
Cuts at: 988 988
Size: 3611
BstBI TT'CG_AA
Cuts at: 1972 1972
Size: 3611
BstXI CCAn_nnnn'nTGG
Cuts at: 267 267
Size: 3611
BstYI r'GATC_y
Cuts at: 1 636 1103 1126 1461 1707 2719 2805 2817
Size: 635 467 23 335 246 1012 86 12
Cuts at: 2817 3163 1
Size: 346 449
Fragments arranged by size:
1012 635 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3611
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1561 1742 1905 1561
Size: 181 163 3267
Fragments arranged by size:
3267 181 163
BtsI GCAGTG_nn'
Cuts at: 854 854
Size: 3611
DraI TTT'AAA
Cuts at: 610 610
Size: 3611
DrdI GACnn_nn'nnGTC
Cuts at: 1317 2175 1317
Size: 858 2753
EaeI y'GGCC_r
Cuts at: 542 1196 1370 1761 1788 3133 542
Size: 654 174 391 27 1345 1020
Fragments arranged by size:
1345 1020 654 391 174 27
EagI C'GGCC_G
Cuts at: 1196 1196
Size: 3611
EarI CTCTTCn'nnn_
Cuts at: 275 293 326 338 351 1634 1844 275
Size: 18 33 12 13 1283 210 2042
Fragments arranged by size:
2042 1283 210 33 18 13 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 526 1819 2129 2275 3421 526
Size: 1293 310 146 1146 716
Fragments arranged by size:
1293 1146 716 310 146
EcoICRI GAG'CTC
Cuts at: 573 623 573
Size: 50 3561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1868 2615 2883 1436
Size: 432 747 268 2164
Fragments arranged by size:
2164 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3611
EcoO109I rG'GnC_Cy
Cuts at: 228 288 750 228
Size: 60 462 3089
Fragments arranged by size:
3089 462 60
EcoRI G'AATT_C
Cuts at: 615 615
Size: 3611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1630 1630
Size: 3611
FspI TGC'GCA
Cuts at: 1392 1392
Size: 3611
HaeII r_GCGC'y
Cuts at: 417 1045 1293 2315 3294 417
Size: 628 248 1022 979 734
Fragments arranged by size:
1022 979 734 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 176 208 1453 1483 1485 1515 176
Size: 32 1245 30 2 30 2272
Fragments arranged by size:
2272 1245 32 30 30 2
HincII GTy'rAC
Cuts at: 650 650
Size: 3611
KasI G'GCGC_C
Cuts at: 1041 1289 1041
Size: 248 3363
KpnI G_GTAC'C
Cuts at: 631 631
Size: 3611
MluI A'CGCG_T
Cuts at: 3044 3044
Size: 3611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2282 2466 2282
Size: 184 3427
MscI TGG'CCA
Cuts at: 544 1372 544
Size: 828 2783
MslI CAynn'nnrTG
Cuts at: 142 1600 1727 142
Size: 1458 127 2026
Fragments arranged by size:
2026 1458 127
MspA1I CmG'CkG
Cuts at: 477 722 1036 1396 2409 2654 477
Size: 245 314 360 1013 245 1434
Fragments arranged by size:
1434 1013 360 314 245 245
NaeI GCC'GGC
Cuts at: 1792 1792
Size: 3611
NarI GG'CG_CC
Cuts at: 1042 1290 1042
Size: 248 3363
NcoI C'CATG_G
Cuts at: 107 107
Size: 3611
NgoMIV G'CCGG_C
Cuts at: 1790 1790
Size: 3611
NspI r_CATG'y
Cuts at: 664 1164 2071 664
Size: 500 907 2204
Fragments arranged by size:
2204 907 500
PciI A'CATG_T
Cuts at: 2067 2067
Size: 3611
PfoI T'CCnGG_A
Cuts at: 520 520
Size: 3611
PmeI GTTT'AAAC
Cuts at: 610 610
Size: 3611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 3579
PpuMI rG'GwC_Cy
Cuts at: 228 288 228
Size: 60 3551
PstI C_TGCA'G
Cuts at: 658 1343 658
Size: 685 2926
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3611
PvuII CAG'CTG
Cuts at: 477 1036 1396 477
Size: 559 360 2692
Fragments arranged by size:
2692 559 360
RsrII CG'GwC_CG
Cuts at: 1806 1806
Size: 3611
SacI G_AGCT'C
Cuts at: 575 625 575
Size: 50 3561
SalI G'TCGA_C
Cuts at: 648 648
Size: 3611
SanDI GG'GwC_CC
Cuts at: 228 288 228
Size: 60 3551
SapI GCTCTTCn'nnn_
Cuts at: 1634 1844 1634
Size: 210 3401
SbfI CC_TGCA'GG
Cuts at: 658 658
Size: 3611
ScaI AGT'ACT
Cuts at: 3128 3128
Size: 3611
SfcI C'TryA_G
Cuts at: 33 654 1339 2332 2523 33
Size: 621 685 993 191 1121
Fragments arranged by size:
1121 993 685 621 191
SfoI GGC'GCC
Cuts at: 1043 1291 1043
Size: 248 3363
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3611
SmaI CCC'GGG
Cuts at: 213 633 213
Size: 420 3191
SmlI C'TyrA_G
Cuts at: 766 1681 2173 2435 766
Size: 915 492 262 1942
Fragments arranged by size:
1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3611
SphI G_CATG'C
Cuts at: 664 1164 664
Size: 500 3111
StuI AGG'CCT
Cuts at: 466 466
Size: 3611
StyI C'CwwG_G
Cuts at: 107 221 745 1099 107
Size: 114 524 354 2619
Fragments arranged by size:
2619 524 354 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 583 1194 1327 1786 1995 583
Size: 611 133 459 209 2199
Fragments arranged by size:
2199 611 459 209 133
TatI w'GTAC_w
Cuts at: 3126 3126
Size: 3611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3579
Tth111I GACn'n_nGTC
Cuts at: 154 1408 154
Size: 1254 2357
XbaI T'CTAG_A
Cuts at: 642 642
Size: 3611
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1106 1106
Size: 3611
XmaI C'CCGG_G
Cuts at: 211 631 211
Size: 420 3191
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI