Restriction map for
pF1KB6833
(Circular) (Six-base) MAPSORT of: pF1KB6833.seq Check: 1586 from: 1 to: 3620
pF1KB6833 3620 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 658 658
Size: 3620
Acc65I G'GTAC_C
Cuts at: 636 636
Size: 3620
AclI AA'CG_TT
Cuts at: 3460 3460
Size: 3620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1445 1877 2624 2892 292
Size: 1153 432 747 268 1020
Fragments arranged by size:
1153 1020 747 432 268
AfeI AGC'GCT
Cuts at: 594 594
Size: 3620
AflIII A'CryG_T
Cuts at: 972 2076 3053 972
Size: 1104 977 1539
Fragments arranged by size:
1539 1104 977
AgeI A'CCGG_T
Cuts at: 2008 2008
Size: 3620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 164 196 164
Size: 32 3588
AlwNI CAG_nnn'CTG
Cuts at: 399 925 2492 399
Size: 526 1567 1527
Fragments arranged by size:
1567 1527 526
ApaLI G'TGCA_C
Cuts at: 2390 2390
Size: 3620
ApoI r'AATT_y
Cuts at: 624 2973 3229 624
Size: 2349 256 1015
Fragments arranged by size:
2349 1015 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
AvaI C'yCGr_G
Cuts at: 640 640
Size: 3620
BamHI G'GATC_C
Cuts at: 645 645
Size: 3620
BanI G'GyrC_C
Cuts at: 463 636 1050 1298 1333 463
Size: 173 414 248 35 2750
Fragments arranged by size:
2750 414 248 173 35
BanII G_rGCy'C
Cuts at: 575 634 1664 575
Size: 59 1030 2531
Fragments arranged by size:
2531 1030 59
BbeI G_GCGC'C
Cuts at: 1054 1302 1054
Size: 248 3372
BciVI GTATCCnnnnn_n'
Cuts at: 1518 2279 3620 1518
Size: 761 1341 1518
Fragments arranged by size:
1518 1341 761
BclI T'GATC_A
Cuts at: 1140 1140
Size: 3620
BglII A'GATC_T
Cuts at: 1135 3172 1135
Size: 2037 1583
BlpI GC'TnA_GC
Cuts at: 732 732
Size: 3620
Bme1580I G_kGCm'C
Cuts at: 537 602 1245 1338 2394 537
Size: 65 643 93 1056 1763
Fragments arranged by size:
1763 1056 643 93 65
BmgBI CAC'GTC
Cuts at: 405 405
Size: 3620
BmrI ACTGGGnnnn_n'
Cuts at: 832 913 1248 3392 832
Size: 81 335 2144 1060
Fragments arranged by size:
2144 1060 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1987 1987
Size: 3620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1675 2167 2465 796
Size: 879 492 298 1951
Fragments arranged by size:
1951 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3324 3324
Size: 3620
BsaAI yAC'GTr
Cuts at: 973 973
Size: 3620
BsaBI GATnn'nnATC
Cuts at: 6 1134 3171 6
Size: 1128 2037 455
Fragments arranged by size:
2037 1128 455
BsaHI Gr'CG_yC
Cuts at: 452 1051 1299 2001 3497 452
Size: 599 248 702 1496 575
Fragments arranged by size:
1496 702 599 575 248
BsaWI w'CCGG_w
Cuts at: 809 1330 2008 2282 2429 2840 809
Size: 521 678 274 147 411 1589
Fragments arranged by size:
1589 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 565 307
Size: 258 3362
BseYI C'CCAG_C
Cuts at: 581 595 1045 1803 2380 2876 581
Size: 14 450 758 577 496 1325
Fragments arranged by size:
1325 758 577 496 450 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 226 301 141
Size: 85 75 3460
Fragments arranged by size:
3460 85 75
BsiEI CG_ry'CG
Cuts at: 104 345 449 1162 1208 2416 104
Size: 241 104 713 46 1208 1308
Fragments arranged by size:
1308 1208 713 241 104 46
BsiHKAI G_wGCw'C
Cuts at: 311 575 634 1412 1602 2394 311
Size: 264 59 778 190 792 1537
Fragments arranged by size:
1537 792 778 264 190 59
Bsp1286I G_dGCh'C
Cuts at: 311 537 575 602 634 1245 1338 1412 1602
Size: 226 38 27 32 611 93 74 190
Cuts at: 1602 1664 2394 311
Size: 62 730 1537
Fragments arranged by size:
1537 730 611 226 190 93 74 62
38 32 27
BspEI T'CCGG_A
Cuts at: 2840 2840
Size: 3620
BspHI T'CATG_A
Cuts at: 2796 2796
Size: 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 670 884 1186 1567 670
Size: 214 302 381 2723
Fragments arranged by size:
2723 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1912 1966 3450 3615 50
Size: 1862 54 1484 165 55
Fragments arranged by size:
1862 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1532 1532
Size: 3620
BsrFI r'CCGG_y
Cuts at: 160 372 1618 1799 2008 160
Size: 212 1246 181 209 1772
Fragments arranged by size:
1772 1246 212 209 181
BssSI C'ACGA_G
Cuts at: 1891 2249 1891
Size: 358 3262
BstAPI GCAn_nnn'nTGC
Cuts at: 997 997
Size: 3620
BstBI TT'CG_AA
Cuts at: 1981 1981
Size: 3620
BstYI r'GATC_y
Cuts at: 1 645 1112 1135 1470 1716 2728 2814 2826
Size: 644 467 23 335 246 1012 86 12
Cuts at: 2826 3172 1
Size: 346 449
Fragments arranged by size:
1012 644 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 149 383 471 149
Size: 234 88 3298
Fragments arranged by size:
3298 234 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1570 1751 1914 1570
Size: 181 163 3276
Fragments arranged by size:
3276 181 163
BtsI GCAGTG_nn'
Cuts at: 863 863
Size: 3620
DraI TTT'AAA
Cuts at: 619 619
Size: 3620
DrdI GACnn_nn'nnGTC
Cuts at: 1326 2184 1326
Size: 858 2762
EaeI y'GGCC_r
Cuts at: 185 342 380 446 1205 1379 1770 1797 3142
Size: 157 38 66 759 174 391 27 1345
Cuts at: 3142 185
Size: 663
Fragments arranged by size:
1345 759 663 391 174 157 66 38
27
EagI C'GGCC_G
Cuts at: 342 446 1205 342
Size: 104 759 2757
Fragments arranged by size:
2757 759 104
EarI CTCTTCn'nnn_
Cuts at: 148 1643 1853 148
Size: 1495 210 1915
Fragments arranged by size:
1915 1495 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1828 2138 2284 3430 1828
Size: 310 146 1146 2018
Fragments arranged by size:
2018 1146 310 146
EcoICRI GAG'CTC
Cuts at: 573 632 573
Size: 59 3561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1445 1877 2624 2892 292
Size: 1153 432 747 268 1020
Fragments arranged by size:
1153 1020 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 759 759
Size: 3620
EcoRI G'AATT_C
Cuts at: 624 624
Size: 3620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 1639
Size: 3620
FspI TGC'GCA
Cuts at: 412 1401 412
Size: 989 2631
FspAI rTGC'GCAy
Cuts at: 412 412
Size: 3620
HaeII r_GCGC'y
Cuts at: 240 271 427 596 1054 1302 2324 3303 240
Size: 31 156 169 458 248 1022 979 557
Fragments arranged by size:
1022 979 557 458 248 169 156 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1462 1492 1494 1524 1462
Size: 30 2 30 3558
Fragments arranged by size:
3558 30 30 2
HincII GTy'rAC
Cuts at: 659 659
Size: 3620
KasI G'GCGC_C
Cuts at: 1050 1298 1050
Size: 248 3372
KpnI G_GTAC'C
Cuts at: 640 640
Size: 3620
MluI A'CGCG_T
Cuts at: 3053 3053
Size: 3620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2475 2291
Size: 184 3436
MscI TGG'CCA
Cuts at: 382 1381 382
Size: 999 2621
MslI CAynn'nnrTG
Cuts at: 1609 1736 1609
Size: 127 3493
MspA1I CmG'CkG
Cuts at: 151 210 473 731 1045 1405 2418 2663 151
Size: 59 263 258 314 360 1013 245 1108
Fragments arranged by size:
1108 1013 360 314 263 258 245 59
NaeI GCC'GGC
Cuts at: 1801 1801
Size: 3620
NarI GG'CG_CC
Cuts at: 1051 1299 1051
Size: 248 3372
NcoI C'CATG_G
Cuts at: 383 383
Size: 3620
NgoMIV G'CCGG_C
Cuts at: 1799 1799
Size: 3620
NspI r_CATG'y
Cuts at: 673 1173 2080 673
Size: 500 907 2213
Fragments arranged by size:
2213 907 500
PciI A'CATG_T
Cuts at: 2076 2076
Size: 3620
PflMI CCAn_nnn'nTGG
Cuts at: 389 389
Size: 3620
PmeI GTTT'AAAC
Cuts at: 619 619
Size: 3620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 3588
PstI C_TGCA'G
Cuts at: 667 1352 667
Size: 685 2935
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3620
PvuII CAG'CTG
Cuts at: 1045 1405 1045
Size: 360 3260
RsrII CG'GwC_CG
Cuts at: 1815 1815
Size: 3620
SacI G_AGCT'C
Cuts at: 575 634 575
Size: 59 3561
SacII CC_GC'GG
Cuts at: 152 474 152
Size: 322 3298
SalI G'TCGA_C
Cuts at: 657 657
Size: 3620
SapI GCTCTTCn'nnn_
Cuts at: 1643 1853 1643
Size: 210 3410
SbfI CC_TGCA'GG
Cuts at: 667 667
Size: 3620
ScaI AGT'ACT
Cuts at: 3137 3137
Size: 3620
SfcI C'TryA_G
Cuts at: 33 663 1348 2341 2532 33
Size: 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191
SfoI GGC'GCC
Cuts at: 1052 1300 1052
Size: 248 3372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3620
SgrAI Cr'CCGG_yG
Cuts at: 160 160
Size: 3620
SmaI CCC'GGG
Cuts at: 642 642
Size: 3620
SmlI C'TyrA_G
Cuts at: 775 1690 2182 2444 775
Size: 915 492 262 1951
Fragments arranged by size:
1951 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3620
SphI G_CATG'C
Cuts at: 673 1173 673
Size: 500 3120
StuI AGG'CCT
Cuts at: 286 286
Size: 3620
StyI C'CwwG_G
Cuts at: 383 754 1108 383
Size: 371 354 2895
Fragments arranged by size:
2895 371 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1203 1336 1795 2004 1203
Size: 133 459 209 2819
Fragments arranged by size:
2819 459 209 133
TatI w'GTAC_w
Cuts at: 3135 3135
Size: 3620
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3588
Tth111I GACn'n_nGTC
Cuts at: 1417 1417
Size: 3620
XbaI T'CTAG_A
Cuts at: 651 651
Size: 3620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1115 114
Size: 1001 2619
XmaI C'CCGG_G
Cuts at: 640 640
Size: 3620
XmnI GAAnn'nnTTC
Cuts at: 253 253
Size: 3620
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI ZraI