Restriction map for
pF1KB6834
(Circular) (Six-base) MAPSORT of: pF1KB6834.seq Check: 2110 from: 1 to: 3680
pF1KB6834 3680 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 225 225
Size: 3680
AccI GT'mk_AC
Cuts at: 718 718
Size: 3680
Acc65I G'GTAC_C
Cuts at: 696 696
Size: 3680
AclI AA'CG_TT
Cuts at: 3520 3520
Size: 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 1937 2684 2952 1505
Size: 432 747 268 2233
Fragments arranged by size:
2233 747 432 268
AflIII A'CryG_T
Cuts at: 578 1032 2136 3113 578
Size: 454 1104 977 1145
Fragments arranged by size:
1145 1104 977 454
AgeI A'CCGG_T
Cuts at: 2068 2068
Size: 3680
AleI CACnn'nnGTG
Cuts at: 583 583
Size: 3680
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 3680
AlwNI CAG_nnn'CTG
Cuts at: 985 2552 985
Size: 1567 2113
ApaLI G'TGCA_C
Cuts at: 2450 2450
Size: 3680
ApoI r'AATT_y
Cuts at: 684 3033 3289 684
Size: 2349 256 1075
Fragments arranged by size:
2349 1075 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3680
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
AvaI C'yCGr_G
Cuts at: 700 700
Size: 3680
BamHI G'GATC_C
Cuts at: 705 705
Size: 3680
BanI G'GyrC_C
Cuts at: 136 696 1110 1358 1393 136
Size: 560 414 248 35 2423
Fragments arranged by size:
2423 560 414 248 35
BanII G_rGCy'C
Cuts at: 694 1724 694
Size: 1030 2650
BbeI G_GCGC'C
Cuts at: 140 1114 1362 140
Size: 974 248 2458
Fragments arranged by size:
2458 974 248
BciVI GTATCCnnnnn_n'
Cuts at: 270 1578 2339 3680 270
Size: 1308 761 1341 270
Fragments arranged by size:
1341 1308 761 270
BclI T'GATC_A
Cuts at: 1200 1200
Size: 3680
BglII A'GATC_T
Cuts at: 1195 3232 1195
Size: 2037 1643
BlpI GC'TnA_GC
Cuts at: 792 792
Size: 3680
Bme1580I G_kGCm'C
Cuts at: 437 1305 1398 2454 437
Size: 868 93 1056 1663
Fragments arranged by size:
1663 1056 868 93
BmgBI CAC'GTC
Cuts at: 257 432 257
Size: 175 3505
BmrI ACTGGGnnnn_n'
Cuts at: 892 973 1308 3452 892
Size: 81 335 2144 1120
Fragments arranged by size:
2144 1120 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 3680
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 3680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1735 2227 2525 856
Size: 879 492 298 2011
Fragments arranged by size:
2011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 529 3384 529
Size: 2855 825
BsaAI yAC'GTr
Cuts at: 1033 1033
Size: 3680
BsaBI GATnn'nnATC
Cuts at: 6 1194 3231 6
Size: 1188 2037 455
Fragments arranged by size:
2037 1188 455
BsaHI Gr'CG_yC
Cuts at: 137 1111 1359 2061 3557 137
Size: 974 248 702 1496 260
Fragments arranged by size:
1496 974 702 260 248
BsaWI w'CCGG_w
Cuts at: 227 869 1390 2068 2342 2489 2900 227
Size: 642 521 678 274 147 411 1007
Fragments arranged by size:
1007 678 642 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 307
Size: 3680
BseYI C'CCAG_C
Cuts at: 1105 1863 2440 2936 1105
Size: 758 577 496 1849
Fragments arranged by size:
1849 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 455 455
Size: 3680
BsiEI CG_ry'CG
Cuts at: 104 1222 1268 2476 104
Size: 1118 46 1208 1308
Fragments arranged by size:
1308 1208 1118 46
BsiHKAI G_wGCw'C
Cuts at: 671 694 1472 1662 2454 671
Size: 23 778 190 792 1897
Fragments arranged by size:
1897 792 778 190 23
BsmBI CGTCTCn'nnnn_
Cuts at: 247 247
Size: 3680
Bsp1286I G_dGCh'C
Cuts at: 437 671 694 1305 1398 1472 1662 1724 2454
Size: 234 23 611 93 74 190 62 730
Cuts at: 2454 437
Size: 1663
Fragments arranged by size:
1663 730 611 234 190 93 74 62
23
BspEI T'CCGG_A
Cuts at: 2900 2900
Size: 3680
BspHI T'CATG_A
Cuts at: 2856 2856
Size: 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 225 730 944 1246 1627 225
Size: 505 214 302 381 2278
Fragments arranged by size:
2278 505 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1972 2026 3510 3675 50
Size: 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1592 1592
Size: 3680
BsrFI r'CCGG_y
Cuts at: 139 283 1678 1859 2068 139
Size: 144 1395 181 209 1751
Fragments arranged by size:
1751 1395 209 181 144
BssSI C'ACGA_G
Cuts at: 1951 2309 1951
Size: 358 3322
BstAPI GCAn_nnn'nTGC
Cuts at: 609 656 1057 609
Size: 47 401 3232
Fragments arranged by size:
3232 401 47
BstBI TT'CG_AA
Cuts at: 2041 2041
Size: 3680
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 3680
BstYI r'GATC_y
Cuts at: 1 593 705 1172 1195 1530 1776 2788 2874
Size: 592 112 467 23 335 246 1012 86
Cuts at: 2874 2886 3232 1
Size: 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 246 112
86 23 12
BtgI C'CryG_G
Cuts at: 437 437
Size: 3680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1630 1811 1974 1630
Size: 181 163 3336
Fragments arranged by size:
3336 181 163
BtsI GCAGTG_nn'
Cuts at: 923 923
Size: 3680
DraI TTT'AAA
Cuts at: 679 679
Size: 3680
DraIII CAC_nnn'GTG
Cuts at: 192 192
Size: 3680
DrdI GACnn_nn'nnGTC
Cuts at: 1386 2244 1386
Size: 858 2822
EaeI y'GGCC_r
Cuts at: 285 542 1265 1439 1830 1857 3202 285
Size: 257 723 174 391 27 1345 763
Fragments arranged by size:
1345 763 723 391 257 174 27
EagI C'GGCC_G
Cuts at: 1265 1265
Size: 3680
EarI CTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1888 2198 2344 3490 1888
Size: 310 146 1146 2078
Fragments arranged by size:
2078 1146 310 146
EcoICRI GAG'CTC
Cuts at: 692 692
Size: 3680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 1937 2684 2952 1505
Size: 432 747 268 2233
Fragments arranged by size:
2233 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 501 819 501
Size: 318 3362
EcoRI G'AATT_C
Cuts at: 684 684
Size: 3680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1699 1699
Size: 3680
FspI TGC'GCA
Cuts at: 325 1461 325
Size: 1136 2544
FspAI rTGC'GCAy
Cuts at: 325 325
Size: 3680
HaeII r_GCGC'y
Cuts at: 140 305 1114 1362 2384 3363 140
Size: 165 809 248 1022 979 457
Fragments arranged by size:
1022 979 809 457 248 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1522 1552 1554 1584 1522
Size: 30 2 30 3618
Fragments arranged by size:
3618 30 30 2
HincII GTy'rAC
Cuts at: 719 719
Size: 3680
KasI G'GCGC_C
Cuts at: 136 1110 1358 136
Size: 974 248 2458
Fragments arranged by size:
2458 974 248
KpnI G_GTAC'C
Cuts at: 700 700
Size: 3680
MluI A'CGCG_T
Cuts at: 3113 3113
Size: 3680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 245 2351 2535 245
Size: 2106 184 1390
Fragments arranged by size:
2106 1390 184
MscI TGG'CCA
Cuts at: 1441 1441
Size: 3680
MslI CAynn'nnrTG
Cuts at: 583 1669 1796 583
Size: 1086 127 2467
Fragments arranged by size:
2467 1086 127
MspA1I CmG'CkG
Cuts at: 174 791 1105 1465 2478 2723 174
Size: 617 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 617 360 314 245
NaeI GCC'GGC
Cuts at: 141 285 1861 141
Size: 144 1576 1960
Fragments arranged by size:
1960 1576 144
NarI GG'CG_CC
Cuts at: 137 1111 1359 137
Size: 974 248 2458
Fragments arranged by size:
2458 974 248
NcoI C'CATG_G
Cuts at: 437 437
Size: 3680
NgoMIV G'CCGG_C
Cuts at: 139 283 1859 139
Size: 144 1576 1960
Fragments arranged by size:
1960 1576 144
NspI r_CATG'y
Cuts at: 324 733 1233 2140 324
Size: 409 500 907 1864
Fragments arranged by size:
1864 907 500 409
PciI A'CATG_T
Cuts at: 2136 2136
Size: 3680
PflMI CCAn_nnn'nTGG
Cuts at: 192 192
Size: 3680
PfoI T'CCnGG_A
Cuts at: 272 272
Size: 3680
PmeI GTTT'AAAC
Cuts at: 679 679
Size: 3680
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 3648
PpuMI rG'GwC_Cy
Cuts at: 501 501
Size: 3680
PstI C_TGCA'G
Cuts at: 727 1412 727
Size: 685 2995
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3680
PvuII CAG'CTG
Cuts at: 174 1105 1465 174
Size: 931 360 2389
Fragments arranged by size:
2389 931 360
RsrII CG'GwC_CG
Cuts at: 1875 1875
Size: 3680
SacI G_AGCT'C
Cuts at: 694 694
Size: 3680
SalI G'TCGA_C
Cuts at: 717 717
Size: 3680
SapI GCTCTTCn'nnn_
Cuts at: 1703 1913 1703
Size: 210 3470
SbfI CC_TGCA'GG
Cuts at: 727 727
Size: 3680
ScaI AGT'ACT
Cuts at: 3197 3197
Size: 3680
SfcI C'TryA_G
Cuts at: 33 212 723 1408 2401 2592 33
Size: 179 511 685 993 191 1121
Fragments arranged by size:
1121 993 685 511 191 179
SfoI GGC'GCC
Cuts at: 138 1112 1360 138
Size: 974 248 2458
Fragments arranged by size:
2458 974 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3680
SgrAI Cr'CCGG_yG
Cuts at: 139 139
Size: 3680
SmaI CCC'GGG
Cuts at: 702 702
Size: 3680
SmlI C'TyrA_G
Cuts at: 835 1750 2242 2504 835
Size: 915 492 262 2011
Fragments arranged by size:
2011 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3680
SphI G_CATG'C
Cuts at: 733 1233 733
Size: 500 3180
StyI C'CwwG_G
Cuts at: 437 814 1168 437
Size: 377 354 2949
Fragments arranged by size:
2949 377 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1396 1855 2064 1263
Size: 133 459 209 2879
Fragments arranged by size:
2879 459 209 133
TatI w'GTAC_w
Cuts at: 296 317 392 3195 296
Size: 21 75 2803 781
Fragments arranged by size:
2803 781 75 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 444 476 3197 3229 444
Size: 32 2721 32 895
Fragments arranged by size:
2721 895 32 32
Tth111I GACn'n_nGTC
Cuts at: 1477 1477
Size: 3680
XbaI T'CTAG_A
Cuts at: 711 711
Size: 3680
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 1175 444
Size: 731 2949
XmaI C'CCGG_G
Cuts at: 700 700
Size: 3680
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI