Restriction map for
pF1KB6840
(Circular) (Six-base) MAPSORT of: pF1KB6840.seq Check: 4595 from: 1 to: 3686
pF1KB6840 3686 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 353 353
Size: 3686
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1511 1943 2690 2958 325
Size: 1186 432 747 268 1053
Fragments arranged by size:
1186 1053 747 432 268
AflIII A'CryG_T
Cuts at: 347 1038 2142 3119 347
Size: 691 1104 977 914
Fragments arranged by size:
1104 977 914 691
AgeI A'CCGG_T
Cuts at: 613 2074 613
Size: 1461 2225
AleI CACnn'nnGTG
Cuts at: 217 217
Size: 3686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 334 334
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 657 991 2558 657
Size: 334 1567 1785
Fragments arranged by size:
1785 1567 334
ApaI G_GGCC'C
Cuts at: 569 569
Size: 3686
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 484 690 3039 3295 484
Size: 206 2349 256 875
Fragments arranged by size:
2349 875 256 206
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 395 455 524 706 395
Size: 60 69 182 3375
Fragments arranged by size:
3375 182 69 60
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 318 702 1116 1364 1399 318
Size: 384 414 248 35 2605
Fragments arranged by size:
2605 414 384 248 35
BanII G_rGCy'C
Cuts at: 156 569 700 1730 156
Size: 413 131 1030 2112
Fragments arranged by size:
2112 1030 413 131
BbeI G_GCGC'C
Cuts at: 1120 1368 1120
Size: 248 3438
BbsI GAAGACnn'nnnn_
Cuts at: 495 495
Size: 3686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 140 174 204 238 140
Size: 34 30 34 3588
Fragments arranged by size:
3588 34 34 30
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 1201 3238 1201
Size: 2037 1649
BlpI GC'TnA_GC
Cuts at: 798 798
Size: 3686
Bme1580I G_kGCm'C
Cuts at: 323 569 1311 1404 2460 323
Size: 246 742 93 1056 1549
Fragments arranged by size:
1549 1056 742 246 93
BmgBI CAC'GTC
Cuts at: 465 465
Size: 3686
BmrI ACTGGGnnnn_n'
Cuts at: 318 898 979 1314 3458 318
Size: 580 81 335 2144 546
Fragments arranged by size:
2144 580 546 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1741 2233 2531 862
Size: 879 492 298 2017
Fragments arranged by size:
2017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 271 3390 271
Size: 3119 567
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 1200 3237 6
Size: 1194 2037 455
Fragments arranged by size:
2037 1194 455
BsaHI Gr'CG_yC
Cuts at: 1117 1365 2067 3563 1117
Size: 248 702 1496 1240
Fragments arranged by size:
1496 1240 702 248
BsaWI w'CCGG_w
Cuts at: 613 875 1396 2074 2348 2495 2906 613
Size: 262 521 678 274 147 411 1393
Fragments arranged by size:
1393 678 521 411 274 262 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 499
Size: 30 3656
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 568 613 313
Size: 255 45 3386
Fragments arranged by size:
3386 255 45
BseYI C'CCAG_C
Cuts at: 1111 1869 2446 2942 1111
Size: 758 577 496 1855
Fragments arranged by size:
1855 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 418 700 1478 1668 2460 418
Size: 282 778 190 792 1644
Fragments arranged by size:
1644 792 778 282 190
Bsp1286I G_dGCh'C
Cuts at: 156 323 418 569 700 1311 1404 1478 1668
Size: 167 95 151 131 611 93 74 190
Cuts at: 1668 1730 2460 156
Size: 62 730 1382
Fragments arranged by size:
1382 730 611 190 167 151 131 95
93 74 62
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 489 736 950 1252 1633 353
Size: 136 247 214 302 381 2406
Fragments arranged by size:
2406 381 302 247 214 136
BsrBI CCG'CTC
Cuts at: 50 1978 2032 3516 3681 50
Size: 1928 54 1484 165 55
Fragments arranged by size:
1928 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 358 613 1684 1865 2074 358
Size: 255 1071 181 209 1970
Fragments arranged by size:
1970 1071 255 209 181
BssSI C'ACGA_G
Cuts at: 149 1957 2315 149
Size: 1808 358 1520
Fragments arranged by size:
1808 1520 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1063 1063
Size: 3686
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstXI CCAn_nnnn'nTGG
Cuts at: 338 338
Size: 3686
BstYI r'GATC_y
Cuts at: 1 711 1178 1201 1536 1782 2794 2880 2892
Size: 710 467 23 335 246 1012 86 12
Cuts at: 2892 3238 1
Size: 346 449
Fragments arranged by size:
1012 710 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 173 635 107
Size: 66 462 3158
Fragments arranged by size:
3158 462 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1636 1817 1980 1636
Size: 181 163 3342
Fragments arranged by size:
3342 181 163
BtsI GCAGTG_nn'
Cuts at: 418 929 418
Size: 511 3175
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 1271 1445 1836 1863 3208 1271
Size: 174 391 27 1345 1749
Fragments arranged by size:
1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 586 595 637 1709 1919 586
Size: 9 42 1072 210 2353
Fragments arranged by size:
2353 1072 210 42 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1894 2204 2350 3496 1894
Size: 310 146 1146 2084
Fragments arranged by size:
2084 1146 310 146
EcoICRI GAG'CTC
Cuts at: 698 698
Size: 3686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1511 1943 2690 2958 325
Size: 1186 432 747 268 1053
Fragments arranged by size:
1186 1053 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 566 573 825 566
Size: 7 252 3427
Fragments arranged by size:
3427 252 7
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1705 1705
Size: 3686
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 558 1120 1368 2390 3369 558
Size: 562 248 1022 979 875
Fragments arranged by size:
1022 979 875 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1558 1560 1590 1528
Size: 30 2 30 3624
Fragments arranged by size:
3624 30 30 2
HincII GTy'rAC
Cuts at: 345 725 345
Size: 380 3306
KasI G'GCGC_C
Cuts at: 1116 1364 1116
Size: 248 3438
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 178 217 336 1675 1802 178
Size: 39 119 1339 127 2062
Fragments arranged by size:
2062 1339 127 119 39
MspA1I CmG'CkG
Cuts at: 355 797 1111 1471 2484 2729 355
Size: 442 314 360 1013 245 1312
Fragments arranged by size:
1312 1013 442 360 314 245
NaeI GCC'GGC
Cuts at: 360 1867 360
Size: 1507 2179
NarI GG'CG_CC
Cuts at: 1117 1365 1117
Size: 248 3438
NcoI C'CATG_G
Cuts at: 107 173 635 107
Size: 66 462 3158
Fragments arranged by size:
3158 462 66
NgoMIV G'CCGG_C
Cuts at: 358 1865 358
Size: 1507 2179
NspI r_CATG'y
Cuts at: 739 1239 2146 739
Size: 500 907 2279
Fragments arranged by size:
2279 907 500
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PspOMI G'GGCC_C
Cuts at: 565 565
Size: 3686
PstI C_TGCA'G
Cuts at: 733 1418 733
Size: 685 3001
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 355 1111 1471 355
Size: 756 360 2570
Fragments arranged by size:
2570 756 360
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 700 700
Size: 3686
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 637 1709 1919 637
Size: 1072 210 2404
Fragments arranged by size:
2404 1072 210
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 729 1414 2407 2598 33
Size: 696 685 993 191 1121
Fragments arranged by size:
1121 993 696 685 191
SfoI GGC'GCC
Cuts at: 1118 1366 1118
Size: 248 3438
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 457 708 457
Size: 251 3435
SmlI C'TyrA_G
Cuts at: 841 1756 2248 2510 841
Size: 915 492 262 2017
Fragments arranged by size:
2017 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
SrfI GCCC'GGGC
Cuts at: 457 457
Size: 3686
StyI C'CwwG_G
Cuts at: 107 173 635 820 1174 107
Size: 66 462 185 354 2619
Fragments arranged by size:
2619 462 354 185 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 154 1269 1402 1861 2070 154
Size: 1115 133 459 209 1770
Fragments arranged by size:
1770 1115 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 247 279 3203 3235 247
Size: 32 2924 32 698
Fragments arranged by size:
2924 698 32 32
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1181 1181
Size: 3686
XmaI C'CCGG_G
Cuts at: 455 706 455
Size: 251 3435
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BpmI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI