Restriction map for
pF1KB6841
(Circular) (Six-base) MAPSORT of: pF1KB6841.seq Check: 5299 from: 1 to: 3629
pF1KB6841 3629 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 410 485 410
Size: 75 3554
AccI GT'mk_AC
Cuts at: 667 667
Size: 3629
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1454 1886 2633 2901 1454
Size: 432 747 268 2182
Fragments arranged by size:
2182 747 432 268
AflIII A'CryG_T
Cuts at: 115 981 2085 3062 115
Size: 866 1104 977 682
Fragments arranged by size:
1104 977 866 682
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AhdI GACnn_n'nnGTC
Cuts at: 567 567
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 266 486 934 2501 266
Size: 220 448 1567 1394
Fragments arranged by size:
1567 1394 448 220
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 633 2982 3238 633
Size: 2349 256 1024
Fragments arranged by size:
2349 1024 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 407 511 607 649 407
Size: 104 96 42 3387
Fragments arranged by size:
3387 104 96 42
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 526 585 645 1059 1307 1342 526
Size: 59 60 414 248 35 2813
Fragments arranged by size:
2813 414 248 60 59 35
BanII G_rGCy'C
Cuts at: 320 512 608 643 1673 320
Size: 192 96 35 1030 2276
Fragments arranged by size:
2276 1030 192 96 35
BbeI G_GCGC'C
Cuts at: 530 589 1063 1311 530
Size: 59 474 248 2848
Fragments arranged by size:
2848 474 248 59
BbvCI CC'TCA_GC
Cuts at: 430 430
Size: 3629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 398 364
Size: 34 3595
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglI GCCn_nnn'nGGC
Cuts at: 583 583
Size: 3629
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 1254 1347 2403 1254
Size: 93 1056 2480
Fragments arranged by size:
2480 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 841 922 1257 3401 841
Size: 81 335 2144 1069
Fragments arranged by size:
2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3629
Bpu10I CC'TnA_GC
Cuts at: 430 430
Size: 3629
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 389 3333 389
Size: 2944 685
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 236 527 586 1060 1308 2010 3506 236
Size: 291 59 474 248 702 1496 359
Fragments arranged by size:
1496 702 474 359 291 248 59
BsaWI w'CCGG_w
Cuts at: 818 1339 2017 2291 2438 2849 818
Size: 521 678 274 147 411 1598
Fragments arranged by size:
1598 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 625 625
Size: 3629
BseYI C'CCAG_C
Cuts at: 245 536 595 1054 1812 2389 2885 245
Size: 291 59 459 758 577 496 989
Fragments arranged by size:
989 758 577 496 459 291 59
BsiEI CG_ry'CG
Cuts at: 104 524 1171 1217 2425 104
Size: 420 647 46 1208 1308
Fragments arranged by size:
1308 1208 647 420 46
BsiHKAI G_wGCw'C
Cuts at: 643 1421 1611 2403 643
Size: 778 190 792 1869
Fragments arranged by size:
1869 792 778 190
BsmI GAATG_Cn'
Cuts at: 556 556
Size: 3629
BsmBI CGTCTCn'nnnn_
Cuts at: 106 436 106
Size: 330 3299
Bsp1286I G_dGCh'C
Cuts at: 320 512 608 643 1254 1347 1421 1611 1673
Size: 192 96 35 611 93 74 190 62
Cuts at: 1673 2403 320
Size: 730 1546
Fragments arranged by size:
1546 730 611 192 190 96 93 74
62 35
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 485 679 893 1195 1576 410
Size: 75 194 214 302 381 2463
Fragments arranged by size:
2463 381 302 214 194 75
BsrBI CCG'CTC
Cuts at: 50 351 1921 1975 3459 3624 50
Size: 301 1570 54 1484 165 55
Fragments arranged by size:
1570 1484 301 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 123 523 1627 1808 2017 123
Size: 400 1104 181 209 1735
Fragments arranged by size:
1735 1104 400 209 181
BssHII G'CGCG_C
Cuts at: 256 385 256
Size: 129 3500
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 266 288 360 486 1006 266
Size: 22 72 126 520 2889
Fragments arranged by size:
2889 520 126 72 22
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstYI r'GATC_y
Cuts at: 1 654 1121 1144 1479 1725 2737 2823 2835
Size: 653 467 23 335 246 1012 86 12
Cuts at: 2835 3181 1
Size: 346 449
Fragments arranged by size:
1012 653 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 214 214
Size: 3629
BtgI C'CryG_G
Cuts at: 107 199 107
Size: 92 3537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 628 628
Size: 3629
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 521 1214 1388 1779 1806 3151 521
Size: 693 174 391 27 1345 999
Fragments arranged by size:
1345 999 693 391 174 27
EagI C'GGCC_G
Cuts at: 521 1214 521
Size: 693 2936
EarI CTCTTCn'nnn_
Cuts at: 585 1652 1862 585
Size: 1067 210 2352
Fragments arranged by size:
2352 1067 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 142 562 1837 2147 2293 3439 142
Size: 420 1275 310 146 1146 332
Fragments arranged by size:
1275 1146 420 332 310 146
EcoICRI GAG'CTC
Cuts at: 641 641
Size: 3629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1454 1886 2633 2901 511
Size: 943 432 747 268 1239
Fragments arranged by size:
1239 943 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 182 207 768 182
Size: 25 561 3043
Fragments arranged by size:
3043 561 25
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1648 1648
Size: 3629
FspI TGC'GCA
Cuts at: 157 270 1410 157
Size: 113 1140 2376
Fragments arranged by size:
2376 1140 113
HaeII r_GCGC'y
Cuts at: 311 429 498 530 589 1063 1311 2333 3312
Size: 118 69 32 59 474 248 1022 979
Cuts at: 3312 311
Size: 628
Fragments arranged by size:
1022 979 628 474 248 118 69 59
32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 1471 1501 1503 1533 553
Size: 32 886 30 2 30 2649
Fragments arranged by size:
2649 886 32 30 30 2
HincII GTy'rAC
Cuts at: 668 668
Size: 3629
KasI G'GCGC_C
Cuts at: 526 585 1059 1307 526
Size: 59 474 248 2848
Fragments arranged by size:
2848 474 248 59
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 115 3062 115
Size: 2947 682
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2484 2300
Size: 184 3445
MscI TGG'CCA
Cuts at: 1390 1390
Size: 3629
MslI CAynn'nnrTG
Cuts at: 276 1618 1745 276
Size: 1342 127 2160
Fragments arranged by size:
2160 1342 127
MspA1I CmG'CkG
Cuts at: 201 263 300 357 740 1054 1414 2427 2672
Size: 62 37 57 383 314 360 1013 245
Cuts at: 2672 201
Size: 1158
Fragments arranged by size:
1158 1013 383 360 314 245 62 57
37
NaeI GCC'GGC
Cuts at: 525 1810 525
Size: 1285 2344
NarI GG'CG_CC
Cuts at: 527 586 1060 1308 527
Size: 59 474 248 2848
Fragments arranged by size:
2848 474 248 59
NcoI C'CATG_G
Cuts at: 107 107
Size: 3629
NgoMIV G'CCGG_C
Cuts at: 523 1808 523
Size: 1285 2344
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PasI CC'CwG_GG
Cuts at: 168 168
Size: 3629
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PpuMI rG'GwC_Cy
Cuts at: 182 207 182
Size: 25 3604
PspXI vC'TCGA_Gb
Cuts at: 511 511
Size: 3629
PstI C_TGCA'G
Cuts at: 482 676 1361 482
Size: 194 685 2750
Fragments arranged by size:
2750 685 194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 643 643
Size: 3629
SacII CC_GC'GG
Cuts at: 202 202
Size: 3629
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SanDI GG'GwC_CC
Cuts at: 182 207 182
Size: 25 3604
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 482 676 482
Size: 194 3435
ScaI AGT'ACT
Cuts at: 286 3146 286
Size: 2860 769
SfcI C'TryA_G
Cuts at: 33 478 672 1357 2350 2541 33
Size: 445 194 685 993 191 1121
Fragments arranged by size:
1121 993 685 445 194 191
SfoI GGC'GCC
Cuts at: 528 587 1061 1309 528
Size: 59 474 248 2848
Fragments arranged by size:
2848 474 248 59
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 409 651 409
Size: 242 3387
SmlI C'TyrA_G
Cuts at: 511 784 1699 2191 2453 511
Size: 273 915 492 262 1687
Fragments arranged by size:
1687 915 492 273 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StuI AGG'CCT
Cuts at: 502 577 502
Size: 75 3554
StyI C'CwwG_G
Cuts at: 107 572 763 1117 107
Size: 465 191 354 2619
Fragments arranged by size:
2619 465 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1345 1804 2013 1212
Size: 133 459 209 2828
Fragments arranged by size:
2828 459 209 133
TatI w'GTAC_w
Cuts at: 284 3144 284
Size: 2860 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 226 258 3146 3178 226
Size: 32 2888 32 677
Fragments arranged by size:
2888 677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XhoI C'TCGA_G
Cuts at: 511 511
Size: 3629
XmaI C'CCGG_G
Cuts at: 407 649 407
Size: 242 3387
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsaXI BsgI
BsiWI BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PspOMI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI