Restriction map for
pF1KB6844
(Circular) (Six-base) MAPSORT of: pF1KB6844.seq Check: 6183 from: 1 to: 3689
pF1KB6844 3689 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AatII G_ACGT'C
Cuts at: 338 338
Size: 3689
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 398 705 398
Size: 307 3382
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 1514 1946 2693 2961 622
Size: 892 432 747 268 1350
Fragments arranged by size:
1350 892 747 432 268
AflIII A'CryG_T
Cuts at: 1041 2145 3122 1041
Size: 1104 977 1608
Fragments arranged by size:
1608 1104 977
AgeI A'CCGG_T
Cuts at: 2077 2077
Size: 3689
AlwNI CAG_nnn'CTG
Cuts at: 534 994 2561 534
Size: 460 1567 1662
Fragments arranged by size:
1662 1567 460
ApaLI G'TGCA_C
Cuts at: 672 2459 672
Size: 1787 1902
ApoI r'AATT_y
Cuts at: 693 3042 3298 693
Size: 2349 256 1084
Fragments arranged by size:
2349 1084 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 545 709 545
Size: 164 3525
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 142 398 413 448 510 705 1119 1367 1402
Size: 256 15 35 62 195 414 248 35
Cuts at: 1402 142
Size: 2429
Fragments arranged by size:
2429 414 256 248 195 62 35 35
15
BanII G_rGCy'C
Cuts at: 367 703 1733 367
Size: 336 1030 2323
Fragments arranged by size:
2323 1030 336
BbeI G_GCGC'C
Cuts at: 146 1123 1371 146
Size: 977 248 2464
Fragments arranged by size:
2464 977 248
BbsI GAAGACnn'nnnn_
Cuts at: 160 610 672 160
Size: 450 62 3177
Fragments arranged by size:
3177 450 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 663 697 90
Size: 34 539 34 3082
Fragments arranged by size:
3082 539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2348 3689 1587
Size: 761 1341 1587
Fragments arranged by size:
1587 1341 761
BclI T'GATC_A
Cuts at: 167 1209 167
Size: 1042 2647
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3689
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 579 801 579
Size: 222 3467
Bme1580I G_kGCm'C
Cuts at: 451 676 1314 1407 2463 451
Size: 225 638 93 1056 1677
Fragments arranged by size:
1677 1056 638 225 93
BmrI ACTGGGnnnn_n'
Cuts at: 643 901 982 1317 3461 643
Size: 258 81 335 2144 871
Fragments arranged by size:
2144 871 335 258 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 2056 573
Size: 1483 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 592
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1744 2236 2534 865
Size: 879 492 298 2020
Fragments arranged by size:
2020 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 3689
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 143 335 1120 1368 2070 3566 143
Size: 192 785 248 702 1496 266
Fragments arranged by size:
1496 785 702 266 248 192
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2351 2498 2909 878
Size: 521 678 274 147 411 1658
Fragments arranged by size:
1658 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 590 1114 1872 2449 2945 590
Size: 524 758 577 496 1334
Fragments arranged by size:
1334 758 577 524 496
BsiEI CG_ry'CG
Cuts at: 104 303 1231 1277 2485 104
Size: 199 928 46 1208 1308
Fragments arranged by size:
1308 1208 928 199 46
BsiHKAI G_wGCw'C
Cuts at: 580 638 676 703 1481 1671 2463 580
Size: 58 38 27 778 190 792 1806
Fragments arranged by size:
1806 792 778 190 58 38 27
Bsp1286I G_dGCh'C
Cuts at: 367 451 580 638 676 703 1314 1407 1481
Size: 84 129 58 38 27 611 93 74
Cuts at: 1481 1671 1733 2463 367
Size: 190 62 730 1593
Fragments arranged by size:
1593 730 611 190 129 93 84 74
62 58 38 27
BspEI T'CCGG_A
Cuts at: 2909 2909
Size: 3689
BspHI T'CATG_A
Cuts at: 2865 2865
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 739 953 1255 1636 739
Size: 214 302 381 2792
Fragments arranged by size:
2792 381 302 214
BsrBI CCG'CTC
Cuts at: 50 489 1981 2035 3519 3684 50
Size: 439 1492 54 1484 165 55
Fragments arranged by size:
1492 1484 439 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 1687 1868 2077 1687
Size: 181 209 3299
Fragments arranged by size:
3299 209 181
BssSI C'ACGA_G
Cuts at: 1960 2318 1960
Size: 358 3331
BstAPI GCAn_nnn'nTGC
Cuts at: 1066 1066
Size: 3689
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstXI CCAn_nnnn'nTGG
Cuts at: 240 441 240
Size: 201 3488
BstYI r'GATC_y
Cuts at: 1 714 1181 1204 1539 1785 2797 2883 2895
Size: 713 467 23 335 246 1012 86 12
Cuts at: 2895 3241 1
Size: 346 449
Fragments arranged by size:
1012 713 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 146 539 146
Size: 393 3296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 417 932 417
Size: 515 3174
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 198 233 198
Size: 35 3654
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2253 1395
Size: 858 2831
EaeI y'GGCC_r
Cuts at: 310 536 1274 1448 1839 1866 3211 310
Size: 226 738 174 391 27 1345 788
Fragments arranged by size:
1345 788 738 391 226 174 27
EagI C'GGCC_G
Cuts at: 1274 1274
Size: 3689
EarI CTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2207 2353 3499 1897
Size: 310 146 1146 2087
Fragments arranged by size:
2087 1146 310 146
EcoICRI GAG'CTC
Cuts at: 701 701
Size: 3689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 622 1514 1946 2693 2961 592
Size: 30 892 432 747 268 1320
Fragments arranged by size:
1320 892 747 432 268 30
EcoO109I rG'GnC_Cy
Cuts at: 531 597 828 531
Size: 66 231 3392
Fragments arranged by size:
3392 231 66
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 177 1708 177
Size: 1531 2158
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 146 1123 1371 2393 3372 146
Size: 977 248 1022 979 463
Fragments arranged by size:
1022 979 977 463 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 211 220 243 1531 1561 1563 1593 188
Size: 23 9 23 1288 30 2 30 2284
Fragments arranged by size:
2284 1288 30 30 23 23 9 2
HincII GTy'rAC
Cuts at: 261 728 261
Size: 467 3222
KasI G'GCGC_C
Cuts at: 142 1119 1367 142
Size: 977 248 2464
Fragments arranged by size:
2464 977 248
KpnI G_GTAC'C
Cuts at: 402 709 402
Size: 307 3382
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2544 2360
Size: 184 3505
MscI TGG'CCA
Cuts at: 538 1450 538
Size: 912 2777
MslI CAynn'nnrTG
Cuts at: 1678 1805 1678
Size: 127 3562
MspA1I CmG'CkG
Cuts at: 283 594 800 1114 1474 2487 2732 283
Size: 311 206 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 360 314 311 245 206
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 143 1120 1368 143
Size: 977 248 2464
Fragments arranged by size:
2464 977 248
NcoI C'CATG_G
Cuts at: 539 539
Size: 3689
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NspI r_CATG'y
Cuts at: 161 742 1242 2149 161
Size: 581 500 907 1701
Fragments arranged by size:
1701 907 581 500
PciI A'CATG_T
Cuts at: 2145 2145
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2828 2860 2828
Size: 32 3657
PstI C_TGCA'G
Cuts at: 736 1421 736
Size: 685 3004
PvuI CG_AT'CG
Cuts at: 104 303 104
Size: 199 3490
PvuII CAG'CTG
Cuts at: 1114 1474 1114
Size: 360 3329
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 703 703
Size: 3689
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 732 1417 2410 2601 33
Size: 699 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 191
SfoI GGC'GCC
Cuts at: 144 1121 1369 144
Size: 977 248 2464
Fragments arranged by size:
2464 977 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 547 711 547
Size: 164 3525
SmlI C'TyrA_G
Cuts at: 844 1759 2251 2513 844
Size: 915 492 262 2020
Fragments arranged by size:
2020 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StyI C'CwwG_G
Cuts at: 539 823 1177 539
Size: 284 354 3051
Fragments arranged by size:
3051 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 528 1272 1405 1864 2073 528
Size: 744 133 459 209 2144
Fragments arranged by size:
2144 744 459 209 133
TatI w'GTAC_w
Cuts at: 200 3204 200
Size: 3004 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 3657
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 240 441 1184 123
Size: 117 201 743 2628
Fragments arranged by size:
2628 743 201 117
XmaI C'CCGG_G
Cuts at: 545 709 545
Size: 164 3525
ZraI GAC'GTC
Cuts at: 336 336
Size: 3689
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI