Restriction map for
pF1KB6845
(Circular) (Six-base) MAPSORT of: pF1KB6845.seq Check: 785 from: 1 to: 3638
pF1KB6845 3638 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 676 676
Size: 3638
Acc65I G'GTAC_C
Cuts at: 654 654
Size: 3638
AclI AA'CG_TT
Cuts at: 3478 3478
Size: 3638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
AflIII A'CryG_T
Cuts at: 990 2094 3071 990
Size: 1104 977 1557
Fragments arranged by size:
1557 1104 977
AgeI A'CCGG_T
Cuts at: 2026 2026
Size: 3638
AlwNI CAG_nnn'CTG
Cuts at: 943 2510 943
Size: 1567 2071
ApaLI G'TGCA_C
Cuts at: 2408 2408
Size: 3638
ApoI r'AATT_y
Cuts at: 642 2991 3247 642
Size: 2349 256 1033
Fragments arranged by size:
2349 1033 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
AvaI C'yCGr_G
Cuts at: 658 658
Size: 3638
BamHI G'GATC_C
Cuts at: 218 361 663 218
Size: 143 302 3193
Fragments arranged by size:
3193 302 143
BanI G'GyrC_C
Cuts at: 442 654 1068 1316 1351 442
Size: 212 414 248 35 2729
Fragments arranged by size:
2729 414 248 212 35
BanII G_rGCy'C
Cuts at: 425 452 652 1682 425
Size: 27 200 1030 2381
Fragments arranged by size:
2381 1030 200 27
BbeI G_GCGC'C
Cuts at: 1072 1320 1072
Size: 248 3390
BbvCI CC'TCA_GC
Cuts at: 356 356
Size: 3638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3604
BciVI GTATCCnnnnn_n'
Cuts at: 1536 2297 3638 1536
Size: 761 1341 1536
Fragments arranged by size:
1536 1341 761
BclI T'GATC_A
Cuts at: 1158 1158
Size: 3638
BglII A'GATC_T
Cuts at: 300 1153 3190 300
Size: 853 2037 748
Fragments arranged by size:
2037 853 748
BlpI GC'TnA_GC
Cuts at: 750 750
Size: 3638
Bme1580I G_kGCm'C
Cuts at: 445 1263 1356 2412 445
Size: 818 93 1056 1671
Fragments arranged by size:
1671 1056 818 93
BmrI ACTGGGnnnn_n'
Cuts at: 125 850 931 1266 3410 125
Size: 725 81 335 2144 353
Fragments arranged by size:
2144 725 353 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2005 2005
Size: 3638
Bpu10I CC'TnA_GC
Cuts at: 356 356
Size: 3638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 814 1693 2185 2483 513
Size: 301 879 492 298 1668
Fragments arranged by size:
1668 879 492 301 298
BsaI GGTCTCn'nnnn_
Cuts at: 3342 3342
Size: 3638
BsaAI yAC'GTr
Cuts at: 991 991
Size: 3638
BsaBI GATnn'nnATC
Cuts at: 6 299 1152 3189 6
Size: 293 853 2037 455
Fragments arranged by size:
2037 853 455 293
BsaHI Gr'CG_yC
Cuts at: 1069 1317 2019 3515 1069
Size: 248 702 1496 1192
Fragments arranged by size:
1496 1192 702 248
BsaWI w'CCGG_w
Cuts at: 827 1348 2026 2300 2447 2858 827
Size: 521 678 274 147 411 1607
Fragments arranged by size:
1607 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 465 495 465
Size: 30 3608
BseYI C'CCAG_C
Cuts at: 1063 1821 2398 2894 1063
Size: 758 577 496 1807
Fragments arranged by size:
1807 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 197
Size: 3638
BsiEI CG_ry'CG
Cuts at: 104 1180 1226 2434 104
Size: 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 46
BsiHKAI G_wGCw'C
Cuts at: 347 652 1430 1620 2412 347
Size: 305 778 190 792 1573
Fragments arranged by size:
1573 792 778 305 190
Bsp1286I G_dGCh'C
Cuts at: 347 425 445 452 652 1263 1356 1430 1620
Size: 78 20 7 200 611 93 74 190
Cuts at: 1620 1682 2412 347
Size: 62 730 1573
Fragments arranged by size:
1573 730 611 200 190 93 78 74
62 20 7
BspEI T'CCGG_A
Cuts at: 2858 2858
Size: 3638
BspHI T'CATG_A
Cuts at: 2814 2814
Size: 3638
BspMI ACCTGCnnnn'nnnn_
Cuts at: 688 902 1204 1585 688
Size: 214 302 381 2741
Fragments arranged by size:
2741 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1930 1984 3468 3633 50
Size: 1880 54 1484 165 55
Fragments arranged by size:
1880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1550 1550
Size: 3638
BsrFI r'CCGG_y
Cuts at: 1636 1817 2026 1636
Size: 181 209 3248
Fragments arranged by size:
3248 209 181
BssSI C'ACGA_G
Cuts at: 1909 2267 1909
Size: 358 3280
BstAPI GCAn_nnn'nTGC
Cuts at: 1015 1015
Size: 3638
BstBI TT'CG_AA
Cuts at: 1999 1999
Size: 3638
BstEII G'GTnAC_C
Cuts at: 605 605
Size: 3638
BstXI CCAn_nnnn'nTGG
Cuts at: 287 287
Size: 3638
BstYI r'GATC_y
Cuts at: 1 218 300 361 663 1130 1153 1488 1734
Size: 217 82 61 302 467 23 335 246
Cuts at: 1734 2746 2832 2844 3190 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 302 246 217
86 82 61 23 12
BtgI C'CryG_G
Cuts at: 119 119
Size: 3638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1588 1769 1932 1588
Size: 181 163 3294
Fragments arranged by size:
3294 181 163
BtsI GCAGTG_nn'
Cuts at: 255 486 881 255
Size: 231 395 3012
Fragments arranged by size:
3012 395 231
DraI TTT'AAA
Cuts at: 637 637
Size: 3638
DrdI GACnn_nn'nnGTC
Cuts at: 1344 2202 1344
Size: 858 2780
EaeI y'GGCC_r
Cuts at: 537 1223 1397 1788 1815 3160 537
Size: 686 174 391 27 1345 1015
Fragments arranged by size:
1345 1015 686 391 174 27
EagI C'GGCC_G
Cuts at: 1223 1223
Size: 3638
EarI CTCTTCn'nnn_
Cuts at: 379 1661 1871 379
Size: 1282 210 2146
Fragments arranged by size:
2146 1282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1846 2156 2302 3448 1846
Size: 310 146 1146 2036
Fragments arranged by size:
2036 1146 310 146
EcoICRI GAG'CTC
Cuts at: 650 650
Size: 3638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1463 1895 2642 2910 1463
Size: 432 747 268 2191
Fragments arranged by size:
2191 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 777 777
Size: 3638
EcoRI G'AATT_C
Cuts at: 642 642
Size: 3638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 1657
Size: 3638
FspI TGC'GCA
Cuts at: 1419 1419
Size: 3638
HaeII r_GCGC'y
Cuts at: 1072 1320 2342 3321 1072
Size: 248 1022 979 1389
Fragments arranged by size:
1389 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 1480 1510 1512 1542 210
Size: 32 1238 30 2 30 2306
Fragments arranged by size:
2306 1238 32 30 30 2
HincII GTy'rAC
Cuts at: 677 677
Size: 3638
KasI G'GCGC_C
Cuts at: 1068 1316 1068
Size: 248 3390
KpnI G_GTAC'C
Cuts at: 658 658
Size: 3638
MluI A'CGCG_T
Cuts at: 3071 3071
Size: 3638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2309 2493 2309
Size: 184 3454
MscI TGG'CCA
Cuts at: 1399 1399
Size: 3638
MslI CAynn'nnrTG
Cuts at: 260 1627 1754 260
Size: 1367 127 2144
Fragments arranged by size:
2144 1367 127
MspA1I CmG'CkG
Cuts at: 224 749 1063 1423 2436 2681 224
Size: 525 314 360 1013 245 1181
Fragments arranged by size:
1181 1013 525 360 314 245
NaeI GCC'GGC
Cuts at: 1819 1819
Size: 3638
NarI GG'CG_CC
Cuts at: 1069 1317 1069
Size: 248 3390
NcoI C'CATG_G
Cuts at: 119 119
Size: 3638
NgoMIV G'CCGG_C
Cuts at: 1817 1817
Size: 3638
NspI r_CATG'y
Cuts at: 691 1191 2098 691
Size: 500 907 2231
Fragments arranged by size:
2231 907 500
PciI A'CATG_T
Cuts at: 2094 2094
Size: 3638
PfoI T'CCnGG_A
Cuts at: 286 286
Size: 3638
PmeI GTTT'AAAC
Cuts at: 637 637
Size: 3638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2777 2809 2777
Size: 32 3606
PsiI TTA'TAA
Cuts at: 590 590
Size: 3638
PstI C_TGCA'G
Cuts at: 685 1370 685
Size: 685 2953
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3638
PvuII CAG'CTG
Cuts at: 1063 1423 1063
Size: 360 3278
RsrII CG'GwC_CG
Cuts at: 1833 1833
Size: 3638
SacI G_AGCT'C
Cuts at: 652 652
Size: 3638
SalI G'TCGA_C
Cuts at: 675 675
Size: 3638
SapI GCTCTTCn'nnn_
Cuts at: 379 1661 1871 379
Size: 1282 210 2146
Fragments arranged by size:
2146 1282 210
SbfI CC_TGCA'GG
Cuts at: 685 685
Size: 3638
ScaI AGT'ACT
Cuts at: 3155 3155
Size: 3638
SexAI A'CCwGG_T
Cuts at: 332 332
Size: 3638
SfcI C'TryA_G
Cuts at: 33 681 1366 2359 2550 33
Size: 648 685 993 191 1121
Fragments arranged by size:
1121 993 685 648 191
SfoI GGC'GCC
Cuts at: 1070 1318 1070
Size: 248 3390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3638
SmaI CCC'GGG
Cuts at: 660 660
Size: 3638
SmlI C'TyrA_G
Cuts at: 528 793 1708 2200 2462 528
Size: 265 915 492 262 1704
Fragments arranged by size:
1704 915 492 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3638
SphI G_CATG'C
Cuts at: 691 1191 691
Size: 500 3138
StuI AGG'CCT
Cuts at: 244 244
Size: 3638
StyI C'CwwG_G
Cuts at: 119 239 772 1126 119
Size: 120 533 354 2631
Fragments arranged by size:
2631 533 354 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1221 1354 1813 2022 1221
Size: 133 459 209 2837
Fragments arranged by size:
2837 459 209 133
TatI w'GTAC_w
Cuts at: 3153 3153
Size: 3638
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 320 352 353 385 465 497 3155 3187 320
Size: 32 1 32 80 32 2658 32 771
Fragments arranged by size:
2658 771 80 32 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1435 1435
Size: 3638
XbaI T'CTAG_A
Cuts at: 669 669
Size: 3638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1133 1133
Size: 3638
XmaI C'CCGG_G
Cuts at: 658 658
Size: 3638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BfrBI BglI BmgBI
BmtI BpmI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI