Restriction map for
pF1KB6847
(Circular) (Six-base) MAPSORT of: pF1KB6847.seq Check: 3049 from: 1 to: 3641
pF1KB6847 3641 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 294 294
Size: 3641
AccI GT'mk_AC
Cuts at: 293 679 293
Size: 386 3255
Acc65I G'GTAC_C
Cuts at: 657 657
Size: 3641
AclI AA'CG_TT
Cuts at: 3481 3481
Size: 3641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1466 1898 2645 2913 263
Size: 1203 432 747 268 991
Fragments arranged by size:
1203 991 747 432 268
AflIII A'CryG_T
Cuts at: 383 993 2097 3074 383
Size: 610 1104 977 950
Fragments arranged by size:
1104 977 950 610
AgeI A'CCGG_T
Cuts at: 2029 2029
Size: 3641
AlwNI CAG_nnn'CTG
Cuts at: 421 946 2513 421
Size: 525 1567 1549
Fragments arranged by size:
1567 1549 525
ApaLI G'TGCA_C
Cuts at: 2411 2411
Size: 3641
ApoI r'AATT_y
Cuts at: 645 2994 3250 645
Size: 2349 256 1036
Fragments arranged by size:
2349 1036 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3641
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
AvaI C'yCGr_G
Cuts at: 117 464 661 117
Size: 347 197 3097
Fragments arranged by size:
3097 347 197
BamHI G'GATC_C
Cuts at: 666 666
Size: 3641
BanI G'GyrC_C
Cuts at: 373 448 455 657 1071 1319 1354 373
Size: 75 7 202 414 248 35 2660
Fragments arranged by size:
2660 414 248 202 75 35 7
BanII G_rGCy'C
Cuts at: 471 655 1685 471
Size: 184 1030 2427
Fragments arranged by size:
2427 1030 184
BbeI G_GCGC'C
Cuts at: 1075 1323 1075
Size: 248 3393
BbsI GAAGACnn'nnnn_
Cuts at: 269 269
Size: 3641
BciVI GTATCCnnnnn_n'
Cuts at: 1539 2300 3641 1539
Size: 761 1341 1539
Fragments arranged by size:
1539 1341 761
BclI T'GATC_A
Cuts at: 1161 1161
Size: 3641
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3641
BglII A'GATC_T
Cuts at: 1156 3193 1156
Size: 2037 1604
BlpI GC'TnA_GC
Cuts at: 753 753
Size: 3641
Bme1580I G_kGCm'C
Cuts at: 124 453 1266 1359 2415 124
Size: 329 813 93 1056 1350
Fragments arranged by size:
1350 1056 813 329 93
BmrI ACTGGGnnnn_n'
Cuts at: 423 853 934 1269 3413 423
Size: 430 81 335 2144 651
Fragments arranged by size:
2144 651 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1696 2188 2486 817
Size: 879 492 298 1972
Fragments arranged by size:
1972 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3345 3345
Size: 3641
BsaAI yAC'GTr
Cuts at: 994 994
Size: 3641
BsaBI GATnn'nnATC
Cuts at: 6 1155 3192 6
Size: 1149 2037 455
Fragments arranged by size:
2037 1149 455
BsaHI Gr'CG_yC
Cuts at: 1072 1320 2022 3518 1072
Size: 248 702 1496 1195
Fragments arranged by size:
1496 1195 702 248
BsaWI w'CCGG_w
Cuts at: 830 1351 2029 2303 2450 2861 830
Size: 521 678 274 147 411 1610
Fragments arranged by size:
1610 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 511
Size: 3641
BseYI C'CCAG_C
Cuts at: 1066 1824 2401 2897 1066
Size: 758 577 496 1810
Fragments arranged by size:
1810 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 526 1183 1229 2437 104
Size: 422 657 46 1208 1308
Fragments arranged by size:
1308 1208 657 422 46
BsiHKAI G_wGCw'C
Cuts at: 655 1433 1623 2415 655
Size: 778 190 792 1881
Fragments arranged by size:
1881 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 124 453 471 655 1266 1359 1433 1623 1685
Size: 329 18 184 611 93 74 190 62
Cuts at: 1685 2415 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 329 190 184 93 74
62 18
BspEI T'CCGG_A
Cuts at: 2861 2861
Size: 3641
BspHI T'CATG_A
Cuts at: 485 2817 485
Size: 2332 1309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 294 691 905 1207 1588 294
Size: 397 214 302 381 2347
Fragments arranged by size:
2347 397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 371 1933 1987 3471 3636 50
Size: 321 1562 54 1484 165 55
Fragments arranged by size:
1562 1484 321 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1553 1553
Size: 3641
BsrFI r'CCGG_y
Cuts at: 521 525 1639 1820 2029 521
Size: 4 1114 181 209 2133
Fragments arranged by size:
2133 1114 209 181 4
BssSI C'ACGA_G
Cuts at: 1912 2270 1912
Size: 358 3283
BstAPI GCAn_nnn'nTGC
Cuts at: 1018 1018
Size: 3641
BstBI TT'CG_AA
Cuts at: 2002 2002
Size: 3641
BstYI r'GATC_y
Cuts at: 1 344 666 1133 1156 1491 1737 2749 2835
Size: 343 322 467 23 335 246 1012 86
Cuts at: 2835 2847 3193 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 343 335 322 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1591 1772 1935 1591
Size: 181 163 3297
Fragments arranged by size:
3297 181 163
BtsI GCAGTG_nn'
Cuts at: 884 884
Size: 3641
DraI TTT'AAA
Cuts at: 640 640
Size: 3641
DraIII CAC_nnn'GTG
Cuts at: 431 431
Size: 3641
DrdI GACnn_nn'nnGTC
Cuts at: 1347 2205 1347
Size: 858 2783
EaeI y'GGCC_r
Cuts at: 506 523 1226 1400 1791 1818 3163 506
Size: 17 703 174 391 27 1345 984
Fragments arranged by size:
1345 984 703 391 174 27 17
EagI C'GGCC_G
Cuts at: 523 1226 523
Size: 703 2938
EarI CTCTTCn'nnn_
Cuts at: 1664 1874 1664
Size: 210 3431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1849 2159 2305 3451 1849
Size: 310 146 1146 2039
Fragments arranged by size:
2039 1146 310 146
EcoICRI GAG'CTC
Cuts at: 653 653
Size: 3641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1466 1898 2645 2913 263
Size: 1203 432 747 268 991
Fragments arranged by size:
1203 991 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 402 579 780 402
Size: 177 201 3263
Fragments arranged by size:
3263 201 177
EcoRI G'AATT_C
Cuts at: 645 645
Size: 3641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 621 1660 252
Size: 369 1039 2233
Fragments arranged by size:
2233 1039 369
FspI TGC'GCA
Cuts at: 1422 1422
Size: 3641
HaeII r_GCGC'y
Cuts at: 422 1075 1323 2345 3324 422
Size: 653 248 1022 979 739
Fragments arranged by size:
1022 979 739 653 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1483 1513 1515 1545 1483
Size: 30 2 30 3579
Fragments arranged by size:
3579 30 30 2
HincII GTy'rAC
Cuts at: 680 680
Size: 3641
KasI G'GCGC_C
Cuts at: 1071 1319 1071
Size: 248 3393
KpnI G_GTAC'C
Cuts at: 661 661
Size: 3641
MluI A'CGCG_T
Cuts at: 3074 3074
Size: 3641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2312 2496 2312
Size: 184 3457
MscI TGG'CCA
Cuts at: 1402 1402
Size: 3641
MslI CAynn'nnrTG
Cuts at: 490 1630 1757 490
Size: 1140 127 2374
Fragments arranged by size:
2374 1140 127
MspA1I CmG'CkG
Cuts at: 567 752 1066 1426 2439 2684 567
Size: 185 314 360 1013 245 1524
Fragments arranged by size:
1524 1013 360 314 245 185
NaeI GCC'GGC
Cuts at: 523 527 1822 523
Size: 4 1295 2342
Fragments arranged by size:
2342 1295 4
NarI GG'CG_CC
Cuts at: 1072 1320 1072
Size: 248 3393
NgoMIV G'CCGG_C
Cuts at: 521 525 1820 521
Size: 4 1295 2342
Fragments arranged by size:
2342 1295 4
NspI r_CATG'y
Cuts at: 198 387 694 1194 2101 198
Size: 189 307 500 907 1738
Fragments arranged by size:
1738 907 500 307 189
PciI A'CATG_T
Cuts at: 383 2097 383
Size: 1714 1927
PfoI T'CCnGG_A
Cuts at: 364 364
Size: 3641
PmeI GTTT'AAAC
Cuts at: 640 640
Size: 3641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 3609
PpuMI rG'GwC_Cy
Cuts at: 402 579 402
Size: 177 3464
PstI C_TGCA'G
Cuts at: 688 1373 688
Size: 685 2956
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3641
PvuII CAG'CTG
Cuts at: 1066 1426 1066
Size: 360 3281
RsrII CG'GwC_CG
Cuts at: 1836 1836
Size: 3641
SacI G_AGCT'C
Cuts at: 655 655
Size: 3641
SalI G'TCGA_C
Cuts at: 678 678
Size: 3641
SapI GCTCTTCn'nnn_
Cuts at: 1664 1874 1664
Size: 210 3431
SbfI CC_TGCA'GG
Cuts at: 688 688
Size: 3641
ScaI AGT'ACT
Cuts at: 3158 3158
Size: 3641
SfcI C'TryA_G
Cuts at: 33 684 1369 2362 2553 33
Size: 651 685 993 191 1121
Fragments arranged by size:
1121 993 685 651 191
SfoI GGC'GCC
Cuts at: 1073 1321 1073
Size: 248 3393
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
SmaI CCC'GGG
Cuts at: 663 663
Size: 3641
SmlI C'TyrA_G
Cuts at: 796 1711 2203 2465 796
Size: 915 492 262 1972
Fragments arranged by size:
1972 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3641
SphI G_CATG'C
Cuts at: 198 694 1194 198
Size: 496 500 2645
Fragments arranged by size:
2645 500 496
StuI AGG'CCT
Cuts at: 115 115
Size: 3641
StyI C'CwwG_G
Cuts at: 775 1129 775
Size: 354 3287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 436 442 1224 1357 1816 2025 391
Size: 45 6 782 133 459 209 2007
Fragments arranged by size:
2007 782 459 209 133 45 6
TatI w'GTAC_w
Cuts at: 134 3156 134
Size: 3022 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3609
Tth111I GACn'n_nGTC
Cuts at: 1438 1438
Size: 3641
XbaI T'CTAG_A
Cuts at: 672 672
Size: 3641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1136 1136
Size: 3641
XmaI C'CCGG_G
Cuts at: 661 661
Size: 3641
XmnI GAAnn'nnTTC
Cuts at: 337 337
Size: 3641
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI