Restriction map for
pF1KB6848
(Circular) (Six-base) MAPSORT of: pF1KB6848.seq Check: 9588 from: 1 to: 3695
pF1KB6848 3695 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1520 1952 2699 2967 287
Size: 1233 432 747 268 1015
Fragments arranged by size:
1233 1015 747 432 268
AfeI AGC'GCT
Cuts at: 414 414
Size: 3695
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AleI CACnn'nnGTG
Cuts at: 508 508
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 699 3048 3304 699
Size: 2349 256 1090
Fragments arranged by size:
2349 1090 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 137 715 137
Size: 578 3117
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 129 249 709 1739 129
Size: 120 460 1030 2085
Fragments arranged by size:
2085 1030 460 120
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 376 500 376
Size: 124 3571
BbvCI CC'TCA_GC
Cuts at: 162 162
Size: 3695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 357 391 90
Size: 34 233 34 3394
Fragments arranged by size:
3394 233 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BfrBI ATG'CAT
Cuts at: 111 111
Size: 3695
BglI GCCn_nnn'nGGC
Cuts at: 298 298
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 161 1320 1413 2469 161
Size: 1159 93 1056 1387
Fragments arranged by size:
1387 1159 1056 93
BmgBI CAC'GTC
Cuts at: 347 347
Size: 3695
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
Bpu10I CC'TnA_GC
Cuts at: 162 162
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 383 884 1405 2083 2357 2504 2915 383
Size: 501 521 678 274 147 411 1163
Fragments arranged by size:
1163 678 521 501 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 365 395 365
Size: 30 3665
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 367 1237 1283 2491 104
Size: 263 870 46 1208 1308
Fragments arranged by size:
1308 1208 870 263 46
BsiHKAI G_wGCw'C
Cuts at: 129 709 1487 1677 2469 129
Size: 580 778 190 792 1355
Fragments arranged by size:
1355 792 778 580 190
Bsp1286I G_dGCh'C
Cuts at: 129 161 249 709 1320 1413 1487 1677 1739
Size: 32 88 460 611 93 74 190 62
Cuts at: 1739 2469 129
Size: 730 1355
Fragments arranged by size:
1355 730 611 460 190 93 88 74
62 32
BspEI T'CCGG_A
Cuts at: 383 2915 383
Size: 2532 1163
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 338 720 1187 1210 1545 1791 2803 2889
Size: 337 382 467 23 335 246 1012 86
Cuts at: 2889 2901 3247 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 382 346 337 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 255 1645 1826 1989 255
Size: 1390 181 163 1961
Fragments arranged by size:
1961 1390 181 163
BtsI GCAGTG_nn'
Cuts at: 308 373 938 308
Size: 65 565 3065
Fragments arranged by size:
3065 565 65
DraI TTT'AAA
Cuts at: 287 694 287
Size: 407 3288
DrdI GACnn_nn'nnGTC
Cuts at: 471 1401 2259 471
Size: 930 858 1907
Fragments arranged by size:
1907 930 858
EaeI y'GGCC_r
Cuts at: 364 584 1280 1454 1845 1872 3217 364
Size: 220 696 174 391 27 1345 842
Fragments arranged by size:
1345 842 696 391 220 174 27
EagI C'GGCC_G
Cuts at: 364 1280 364
Size: 916 2779
EarI CTCTTCn'nnn_
Cuts at: 403 1718 1928 403
Size: 1315 210 2170
Fragments arranged by size:
2170 1315 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 127 707 127
Size: 580 3115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1520 1952 2699 2967 287
Size: 1233 432 747 268 1015
Fragments arranged by size:
1233 1015 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 834 834
Size: 3695
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 416 1129 1377 2399 3378 416
Size: 713 248 1022 979 733
Fragments arranged by size:
1022 979 733 713 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1537 1567 1569 1599 1537
Size: 30 2 30 3633
Fragments arranged by size:
3633 30 30 2
HincII GTy'rAC
Cuts at: 594 734 594
Size: 140 3555
HindIII A'AGCT_T
Cuts at: 430 430
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 102 2366 2550 102
Size: 2264 184 1247
Fragments arranged by size:
2264 1247 184
MscI TGG'CCA
Cuts at: 586 1456 586
Size: 870 2825
MslI CAynn'nnrTG
Cuts at: 508 580 1684 1811 508
Size: 72 1104 127 2392
Fragments arranged by size:
2392 1104 127 72
MspA1I CmG'CkG
Cuts at: 462 806 1120 1480 2493 2738 462
Size: 344 314 360 1013 245 1419
Fragments arranged by size:
1419 1013 360 344 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NdeI CA'TA_TG
Cuts at: 630 630
Size: 3695
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NsiI A_TGCA'T
Cuts at: 113 113
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PflMI CCAn_nnn'nTGG
Cuts at: 121 121
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 129 709 129
Size: 580 3115
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 403 1718 1928 403
Size: 1315 210 2170
Fragments arranged by size:
2170 1315 210
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2416 2607 33
Size: 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 139 717 139
Size: 578 3117
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StyI C'CwwG_G
Cuts at: 587 829 1183 587
Size: 242 354 3099
Fragments arranged by size:
3099 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 1278 1411 1870 2079 366
Size: 912 133 459 209 1982
Fragments arranged by size:
1982 912 459 209 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 365 373 397 3212 3244 341
Size: 24 8 24 2815 32 792
Fragments arranged by size:
2815 792 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 180 1190 114
Size: 66 1010 2619
Fragments arranged by size:
2619 1010 66
XmaI C'CCGG_G
Cuts at: 137 715 137
Size: 578 3117
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BmtI BpmI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI