Restriction map for
pF1KB6849
(Circular) (Six-base) MAPSORT of: pF1KB6849.seq Check: 5984 from: 1 to: 3644
pF1KB6849 3644 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 682 682
Size: 3644
Acc65I G'GTAC_C
Cuts at: 660 660
Size: 3644
AclI AA'CG_TT
Cuts at: 3484 3484
Size: 3644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1469 1901 2648 2916 1469
Size: 432 747 268 2197
Fragments arranged by size:
2197 747 432 268
AflIII A'CryG_T
Cuts at: 996 2100 3077 996
Size: 1104 977 1563
Fragments arranged by size:
1563 1104 977
AgeI A'CCGG_T
Cuts at: 2032 2032
Size: 3644
AleI CACnn'nnGTG
Cuts at: 190 373 190
Size: 183 3461
AlwNI CAG_nnn'CTG
Cuts at: 150 162 949 2516 150
Size: 12 787 1567 1278
Fragments arranged by size:
1567 1278 787 12
ApaLI G'TGCA_C
Cuts at: 183 2414 183
Size: 2231 1413
ApoI r'AATT_y
Cuts at: 534 648 2997 3253 534
Size: 114 2349 256 925
Fragments arranged by size:
2349 925 256 114
AseI AT'TA_AT
Cuts at: 20 20
Size: 3644
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
AvaI C'yCGr_G
Cuts at: 664 664
Size: 3644
BamHI G'GATC_C
Cuts at: 669 669
Size: 3644
BanI G'GyrC_C
Cuts at: 660 1074 1322 1357 660
Size: 414 248 35 2947
Fragments arranged by size:
2947 414 248 35
BanII G_rGCy'C
Cuts at: 658 1688 658
Size: 1030 2614
BbeI G_GCGC'C
Cuts at: 1078 1326 1078
Size: 248 3396
BbsI GAAGACnn'nnnn_
Cuts at: 118 118
Size: 3644
BciVI GTATCCnnnnn_n'
Cuts at: 1542 2303 3644 1542
Size: 761 1341 1542
Fragments arranged by size:
1542 1341 761
BclI T'GATC_A
Cuts at: 1164 1164
Size: 3644
BglII A'GATC_T
Cuts at: 1159 3196 1159
Size: 2037 1607
BlpI GC'TnA_GC
Cuts at: 756 756
Size: 3644
Bme1580I G_kGCm'C
Cuts at: 187 1269 1362 2418 187
Size: 1082 93 1056 1413
Fragments arranged by size:
1413 1082 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 856 937 1272 3416 856
Size: 81 335 2144 1084
Fragments arranged by size:
2144 1084 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2011 2011
Size: 3644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 312
Size: 3644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1699 2191 2489 820
Size: 879 492 298 1975
Fragments arranged by size:
1975 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 200 594 3348 200
Size: 394 2754 496
Fragments arranged by size:
2754 496 394
BsaAI yAC'GTr
Cuts at: 997 997
Size: 3644
BsaBI GATnn'nnATC
Cuts at: 6 1158 3195 6
Size: 1152 2037 455
Fragments arranged by size:
2037 1152 455
BsaHI Gr'CG_yC
Cuts at: 1075 1323 2025 3521 1075
Size: 248 702 1496 1198
Fragments arranged by size:
1496 1198 702 248
BsaWI w'CCGG_w
Cuts at: 833 1354 2032 2306 2453 2864 833
Size: 521 678 274 147 411 1613
Fragments arranged by size:
1613 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 247 407 1069 1827 2404 2900 247
Size: 160 662 758 577 496 991
Fragments arranged by size:
991 758 662 577 496 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 526 397
Size: 129 3515
BsiEI CG_ry'CG
Cuts at: 104 1186 1232 2440 104
Size: 1082 46 1208 1308
Fragments arranged by size:
1308 1208 1082 46
BsiHKAI G_wGCw'C
Cuts at: 187 658 1436 1626 2418 187
Size: 471 778 190 792 1413
Fragments arranged by size:
1413 792 778 471 190
Bsp1286I G_dGCh'C
Cuts at: 187 658 1269 1362 1436 1626 1688 2418 187
Size: 471 611 93 74 190 62 730 1413
Fragments arranged by size:
1413 730 611 471 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2864 2864
Size: 3644
BspHI T'CATG_A
Cuts at: 308 2820 308
Size: 2512 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 694 908 1210 1591 218
Size: 476 214 302 381 2271
Fragments arranged by size:
2271 476 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1936 1990 3474 3639 50
Size: 1886 54 1484 165 55
Fragments arranged by size:
1886 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1556 1556
Size: 3644
BsrFI r'CCGG_y
Cuts at: 265 1642 1823 2032 265
Size: 1377 181 209 1877
Fragments arranged by size:
1877 1377 209 181
BssSI C'ACGA_G
Cuts at: 1915 2273 1915
Size: 358 3286
BstAPI GCAn_nnn'nTGC
Cuts at: 1021 1021
Size: 3644
BstBI TT'CG_AA
Cuts at: 2005 2005
Size: 3644
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 3644
BstYI r'GATC_y
Cuts at: 1 323 669 1136 1159 1494 1740 2752 2838
Size: 322 346 467 23 335 246 1012 86
Cuts at: 2838 2850 3196 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 346 335 322 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 347 1594 1775 1938 347
Size: 1247 181 163 2053
Fragments arranged by size:
2053 1247 181 163
BtsI GCAGTG_nn'
Cuts at: 887 887
Size: 3644
DraI TTT'AAA
Cuts at: 643 643
Size: 3644
DrdI GACnn_nn'nnGTC
Cuts at: 1350 2208 1350
Size: 858 2786
EaeI y'GGCC_r
Cuts at: 635 1229 1403 1794 1821 3166 635
Size: 594 174 391 27 1345 1113
Fragments arranged by size:
1345 1113 594 391 174 27
EagI C'GGCC_G
Cuts at: 1229 1229
Size: 3644
EarI CTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1852 2162 2308 3454 1852
Size: 310 146 1146 2042
Fragments arranged by size:
2042 1146 310 146
EcoICRI GAG'CTC
Cuts at: 656 656
Size: 3644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 1469 1901 2648 2916 312
Size: 1157 432 747 268 1040
Fragments arranged by size:
1157 1040 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 783 783
Size: 3644
EcoRI G'AATT_C
Cuts at: 648 648
Size: 3644
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3644
FspI TGC'GCA
Cuts at: 1425 1425
Size: 3644
HaeII r_GCGC'y
Cuts at: 151 1078 1326 2348 3327 151
Size: 927 248 1022 979 468
Fragments arranged by size:
1022 979 927 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1486 1516 1518 1548 1486
Size: 30 2 30 3582
Fragments arranged by size:
3582 30 30 2
HincII GTy'rAC
Cuts at: 683 683
Size: 3644
KasI G'GCGC_C
Cuts at: 1074 1322 1074
Size: 248 3396
KpnI G_GTAC'C
Cuts at: 664 664
Size: 3644
MluI A'CGCG_T
Cuts at: 3077 3077
Size: 3644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2499 2315
Size: 184 3460
MscI TGG'CCA
Cuts at: 1405 1405
Size: 3644
MslI CAynn'nnrTG
Cuts at: 190 373 1633 1760 190
Size: 183 1260 127 2074
Fragments arranged by size:
2074 1260 183 127
MspA1I CmG'CkG
Cuts at: 755 1069 1429 2442 2687 755
Size: 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1825 1825
Size: 3644
NarI GG'CG_CC
Cuts at: 1075 1323 1075
Size: 248 3396
NgoMIV G'CCGG_C
Cuts at: 1823 1823
Size: 3644
NspI r_CATG'y
Cuts at: 697 1197 2104 697
Size: 500 907 2237
Fragments arranged by size:
2237 907 500
PciI A'CATG_T
Cuts at: 2100 2100
Size: 3644
PmeI GTTT'AAAC
Cuts at: 643 643
Size: 3644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2783 2815 2783
Size: 32 3612
PstI C_TGCA'G
Cuts at: 215 691 1376 215
Size: 476 685 2483
Fragments arranged by size:
2483 685 476
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3644
PvuII CAG'CTG
Cuts at: 1069 1429 1069
Size: 360 3284
RsrII CG'GwC_CG
Cuts at: 1839 1839
Size: 3644
SacI G_AGCT'C
Cuts at: 658 658
Size: 3644
SalI G'TCGA_C
Cuts at: 681 681
Size: 3644
SapI GCTCTTCn'nnn_
Cuts at: 1667 1877 1667
Size: 210 3434
SbfI CC_TGCA'GG
Cuts at: 691 691
Size: 3644
ScaI AGT'ACT
Cuts at: 3161 3161
Size: 3644
SfcI C'TryA_G
Cuts at: 33 211 687 1372 2365 2556 33
Size: 178 476 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 191 178
SfoI GGC'GCC
Cuts at: 1076 1324 1076
Size: 248 3396
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3644
SmaI CCC'GGG
Cuts at: 666 666
Size: 3644
SmlI C'TyrA_G
Cuts at: 799 1714 2206 2468 799
Size: 915 492 262 1975
Fragments arranged by size:
1975 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3644
SphI G_CATG'C
Cuts at: 697 1197 697
Size: 500 3144
StyI C'CwwG_G
Cuts at: 318 778 1132 318
Size: 460 354 2830
Fragments arranged by size:
2830 460 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 180 559 1227 1360 1819 2028 180
Size: 379 668 133 459 209 1796
Fragments arranged by size:
1796 668 459 379 209 133
TatI w'GTAC_w
Cuts at: 3159 3159
Size: 3644
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3612
Tth111I GACn'n_nGTC
Cuts at: 1441 1441
Size: 3644
XbaI T'CTAG_A
Cuts at: 675 675
Size: 3644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 507 1139 507
Size: 632 3012
XmaI C'CCGG_G
Cuts at: 664 664
Size: 3644
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI