Restriction map for
pF1KB6850
(Circular) (Six-base) MAPSORT of: pF1KB6850.seq Check: 5294 from: 1 to: 3695
pF1KB6850 3695 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 428 1520 1952 2699 2967 401
Size: 27 1092 432 747 268 1129
Fragments arranged by size:
1129 1092 747 432 268 27
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 628 1000 2567 628
Size: 372 1567 1756
Fragments arranged by size:
1756 1567 372
ApaLI G'TGCA_C
Cuts at: 211 2465 211
Size: 2254 1441
ApoI r'AATT_y
Cuts at: 699 3048 3304 699
Size: 2349 256 1090
Fragments arranged by size:
2349 1090 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 504 720 504
Size: 216 3479
BanI G'GyrC_C
Cuts at: 159 711 1125 1373 1408 159
Size: 552 414 248 35 2446
Fragments arranged by size:
2446 552 414 248 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BciVI GTATCCnnnnn_n'
Cuts at: 292 1593 2354 3695 292
Size: 1301 761 1341 292
Fragments arranged by size:
1341 1301 761 292
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 597 1210 3247 597
Size: 613 2037 1045
Fragments arranged by size:
2037 1045 613
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 215 1320 1413 2469 215
Size: 1105 93 1056 1441
Fragments arranged by size:
1441 1105 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 605
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 871 1750 2242 2540 625
Size: 246 879 492 298 1780
Fragments arranged by size:
1780 879 492 298 246
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 3695
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 215 402 709 1487 1677 2469 215
Size: 187 307 778 190 792 1441
Fragments arranged by size:
1441 792 778 307 190 187
Bsp1286I G_dGCh'C
Cuts at: 215 402 709 1320 1413 1487 1677 1739 2469
Size: 187 307 611 93 74 190 62 730
Cuts at: 2469 215
Size: 1441
Fragments arranged by size:
1441 730 611 307 190 187 93 74
62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BsrGI T'GTAC_A
Cuts at: 219 219
Size: 3695
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 504 597 720 1187 1210 1545 1791 2803
Size: 503 93 123 467 23 335 246 1012
Cuts at: 2803 2889 2901 3247 1
Size: 86 12 346 449
Fragments arranged by size:
1012 503 467 449 346 335 246 123
93 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 374 694 374
Size: 320 3375
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 1280 1454 1845 1872 3217 1280
Size: 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 428 605 1520 1952 2699 2967 401
Size: 27 177 915 432 747 268 1129
Fragments arranged by size:
1129 915 747 432 268 177 27
EcoNI CCTnn'n_nnAGG
Cuts at: 154 154
Size: 3695
EcoO109I rG'GnC_Cy
Cuts at: 834 834
Size: 3695
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
EcoRV GAT'ATC
Cuts at: 523 578 523
Size: 55 3640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 1129 1377 2399 3378 1129
Size: 248 1022 979 1446
Fragments arranged by size:
1446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 1537 1567 1569 1599 658
Size: 32 847 30 2 30 2754
Fragments arranged by size:
2754 847 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 309 2366 2550 309
Size: 2057 184 1454
Fragments arranged by size:
2057 1454 184
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 1684 1811 1684
Size: 127 3568
MspA1I CmG'CkG
Cuts at: 806 1120 1480 2493 2738 806
Size: 314 360 1013 245 1763
Fragments arranged by size:
1763 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NcoI C'CATG_G
Cuts at: 107 107
Size: 3695
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PflMI CCAn_nnn'nTGG
Cuts at: 207 207
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 242 457 494 650 738 1423 2416 2607
Size: 209 215 37 156 88 685 993 191
Cuts at: 2607 33
Size: 1121
Fragments arranged by size:
1121 993 685 215 209 191 156 88
37
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 604 850 1765 2257 2519 604
Size: 246 915 492 262 1780
Fragments arranged by size:
1780 915 492 262 246
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
SspI AAT'ATT
Cuts at: 309 309
Size: 3695
StuI AGG'CCT
Cuts at: 341 341
Size: 3695
StyI C'CwwG_G
Cuts at: 107 155 500 829 1183 107
Size: 48 345 329 354 2619
Fragments arranged by size:
2619 354 345 329 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 1278 1411 1870 2079 532
Size: 746 133 459 209 2148
Fragments arranged by size:
2148 746 459 209 133
TatI w'GTAC_w
Cuts at: 219 439 3210 219
Size: 220 2771 704
Fragments arranged by size:
2771 704 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 266 298 3212 3244 266
Size: 32 2914 32 717
Fragments arranged by size:
2914 717 32 32
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI