Restriction map for
pF1KB6854
(Circular) (Six-base) MAPSORT of: pF1KB6854.seq Check: 2808 from: 1 to: 3695
pF1KB6854 3695 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 516 516
Size: 3695
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 427 1520 1952 2699 2967 214
Size: 213 1093 432 747 268 942
Fragments arranged by size:
1093 942 747 432 268 213
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 352 379 352
Size: 27 3668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 526 558 526
Size: 32 3663
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 247 699 3048 3304 247
Size: 452 2349 256 638
Fragments arranged by size:
2349 638 452 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 278 615 715 278
Size: 337 100 3258
Fragments arranged by size:
3258 337 100
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 218 709 1739 218
Size: 491 1030 2174
Fragments arranged by size:
2174 1030 491
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 441 441
Size: 3695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3661
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglI GCCn_nnn'nGGC
Cuts at: 617 617
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2469 1320
Size: 93 1056 2546
Fragments arranged by size:
2546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 689 258
Size: 431 3264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 3399 153
Size: 3246 449
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 315 432 1126 1374 2076 3572 315
Size: 117 694 248 702 1496 438
Fragments arranged by size:
1496 702 694 438 248 117
BsaWI w'CCGG_w
Cuts at: 218 884 1405 2083 2357 2504 2915 218
Size: 666 521 678 274 147 411 998
Fragments arranged by size:
998 678 666 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 694 697 694
Size: 3 3692
BseYI C'CCAG_C
Cuts at: 125 456 661 1120 1878 2455 2951 125
Size: 331 205 459 758 577 496 869
Fragments arranged by size:
869 758 577 496 459 331 205
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 709 1487 1677 2469 709
Size: 778 190 792 1935
Fragments arranged by size:
1935 792 778 190
BsmI GAATG_Cn'
Cuts at: 642 642
Size: 3695
BsmBI CGTCTCn'nnnn_
Cuts at: 184 423 665 184
Size: 239 242 3214
Fragments arranged by size:
3214 242 239
Bsp1286I G_dGCh'C
Cuts at: 218 709 1320 1413 1487 1677 1739 2469 218
Size: 491 611 93 74 190 62 730 1444
Fragments arranged by size:
1444 730 611 491 190 93 74 62
BspEI T'CCGG_A
Cuts at: 218 2915 218
Size: 2697 998
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 745 959 1261 1642 516
Size: 229 214 302 381 2569
Fragments arranged by size:
2569 381 302 229 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 323 2056 323
Size: 1733 1962
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3695
BstYI r'GATC_y
Cuts at: 1 263 720 1187 1210 1545 1791 2803 2889
Size: 262 457 467 23 335 246 1012 86
Cuts at: 2889 2901 3247 1
Size: 12 346 449
Fragments arranged by size:
1012 467 457 449 346 335 262 246
86 23 12
BtgI C'CryG_G
Cuts at: 154 310 154
Size: 156 3539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 257 464 609 1280 1454 1845 1872 3217 257
Size: 207 145 671 174 391 27 1345 735
Fragments arranged by size:
1345 735 671 391 207 174 145 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 622 1718 1928 622
Size: 1096 210 2389
Fragments arranged by size:
2389 1096 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 487 544 1903 2213 2359 3505 487
Size: 57 1359 310 146 1146 677
Fragments arranged by size:
1359 1146 677 310 146 57
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 258 427 689 1520 1952 2699 2967 214
Size: 44 169 262 831 432 747 268 942
Fragments arranged by size:
942 831 747 432 268 262 169 44
EcoO109I rG'GnC_Cy
Cuts at: 306 834 306
Size: 528 3167
EcoRI G'AATT_C
Cuts at: 699 699
Size: 3695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 1714 477
Size: 1237 2458
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 507 1129 1377 2399 3378 507
Size: 622 248 1022 979 824
Fragments arranged by size:
1022 979 824 622 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1537 1567 1569 1599 1537
Size: 30 2 30 3633
Fragments arranged by size:
3633 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2366 2550 2366
Size: 184 3511
MscI TGG'CCA
Cuts at: 259 466 1456 259
Size: 207 990 2498
Fragments arranged by size:
2498 990 207
MslI CAynn'nnrTG
Cuts at: 112 361 1684 1811 112
Size: 249 1323 127 1996
Fragments arranged by size:
1996 1323 249 127
MspA1I CmG'CkG
Cuts at: 156 806 1120 1480 2493 2738 156
Size: 650 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 650 360 314 245
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PpuMI rG'GwC_Cy
Cuts at: 306 306
Size: 3695
PshAI GACnn'nnGTC
Cuts at: 445 445
Size: 3695
PstI C_TGCA'G
Cuts at: 440 742 1427 440
Size: 302 685 2708
Fragments arranged by size:
2708 685 302
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 1120 1480 1120
Size: 360 3335
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SacII CC_GC'GG
Cuts at: 157 157
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 436 738 1423 2416 2607 33
Size: 403 302 685 993 191 1121
Fragments arranged by size:
1121 993 685 403 302 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 617 617
Size: 3695
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 280 617 717 280
Size: 337 100 3258
Fragments arranged by size:
3258 337 100
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
SrfI GCCC'GGGC
Cuts at: 617 617
Size: 3695
StyI C'CwwG_G
Cuts at: 829 1183 829
Size: 354 3341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 213 245 3212 3244 213
Size: 32 2967 32 664
Fragments arranged by size:
2967 664 32 32
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 658 1190 658
Size: 532 3163
XmaI C'CCGG_G
Cuts at: 278 615 715 278
Size: 337 100 3258
Fragments arranged by size:
3258 337 100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspOMI PspXI PsrI SanDI
SexAI SgrAI SnaBI SspI StuI SwaI XhoI XmnI
ZraI