Restriction map for
pF1KB6855
(Circular) (Six-base) MAPSORT of: pF1KB6855.seq Check: 2432 from: 1 to: 3647
pF1KB6855 3647 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 519 519
Size: 3647
AccI GT'mk_AC
Cuts at: 685 685
Size: 3647
Acc65I G'GTAC_C
Cuts at: 663 663
Size: 3647
AclI AA'CG_TT
Cuts at: 641 3487 641
Size: 2846 801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 475 1472 1904 2651 2919 234
Size: 241 997 432 747 268 962
Fragments arranged by size:
997 962 747 432 268 241
AflIII A'CryG_T
Cuts at: 999 2103 3080 999
Size: 1104 977 1566
Fragments arranged by size:
1566 1104 977
AgeI A'CCGG_T
Cuts at: 2035 2035
Size: 3647
AlwNI CAG_nnn'CTG
Cuts at: 952 2519 952
Size: 1567 2080
ApaLI G'TGCA_C
Cuts at: 2417 2417
Size: 3647
ApoI r'AATT_y
Cuts at: 651 3000 3256 651
Size: 2349 256 1042
Fragments arranged by size:
2349 1042 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3647
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
AvaI C'yCGr_G
Cuts at: 667 667
Size: 3647
BamHI G'GATC_C
Cuts at: 672 672
Size: 3647
BanI G'GyrC_C
Cuts at: 175 663 1077 1325 1360 175
Size: 488 414 248 35 2462
Fragments arranged by size:
2462 488 414 248 35
BanII G_rGCy'C
Cuts at: 661 1691 661
Size: 1030 2617
BbeI G_GCGC'C
Cuts at: 1081 1329 1081
Size: 248 3399
BbsI GAAGACnn'nnnn_
Cuts at: 268 463 268
Size: 195 3452
BbvCI CC'TCA_GC
Cuts at: 469 469
Size: 3647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3613
BciVI GTATCCnnnnn_n'
Cuts at: 1545 2306 3647 1545
Size: 761 1341 1545
Fragments arranged by size:
1545 1341 761
BclI T'GATC_A
Cuts at: 1167 1167
Size: 3647
BglII A'GATC_T
Cuts at: 234 1162 3199 234
Size: 928 2037 682
Fragments arranged by size:
2037 928 682
BlpI GC'TnA_GC
Cuts at: 117 759 117
Size: 642 3005
Bme1580I G_kGCm'C
Cuts at: 178 1272 1365 2421 178
Size: 1094 93 1056 1404
Fragments arranged by size:
1404 1094 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 158 174 859 940 1275 3419 158
Size: 16 685 81 335 2144 386
Fragments arranged by size:
2144 685 386 335 81 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 400 340
Size: 60 3587
Bpu10I CC'TnA_GC
Cuts at: 469 469
Size: 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 1702 2194 2492 823
Size: 879 492 298 1978
Fragments arranged by size:
1978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3351 3351
Size: 3647
BsaAI yAC'GTr
Cuts at: 1000 1000
Size: 3647
BsaBI GATnn'nnATC
Cuts at: 6 1161 3198 6
Size: 1155 2037 455
Fragments arranged by size:
2037 1155 455
BsaHI Gr'CG_yC
Cuts at: 1078 1326 2028 3524 1078
Size: 248 702 1496 1201
Fragments arranged by size:
1496 1201 702 248
BsaWI w'CCGG_w
Cuts at: 836 1357 2035 2309 2456 2867 836
Size: 521 678 274 147 411 1616
Fragments arranged by size:
1616 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 3647
BseYI C'CCAG_C
Cuts at: 1072 1830 2407 2903 1072
Size: 758 577 496 1816
Fragments arranged by size:
1816 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 180 180
Size: 3647
BsiEI CG_ry'CG
Cuts at: 104 1189 1235 2443 104
Size: 1085 46 1208 1308
Fragments arranged by size:
1308 1208 1085 46
BsiHKAI G_wGCw'C
Cuts at: 661 1439 1629 2421 661
Size: 778 190 792 1887
Fragments arranged by size:
1887 792 778 190
BsmI GAATG_Cn'
Cuts at: 543 543
Size: 3647
Bsp1286I G_dGCh'C
Cuts at: 178 661 1272 1365 1439 1629 1691 2421 178
Size: 483 611 93 74 190 62 730 1404
Fragments arranged by size:
1404 730 611 483 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2867 2867
Size: 3647
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 3647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 519 697 911 1213 1594 224
Size: 295 178 214 302 381 2277
Fragments arranged by size:
2277 381 302 295 214 178
BsrBI CCG'CTC
Cuts at: 50 1939 1993 3477 3642 50
Size: 1889 54 1484 165 55
Fragments arranged by size:
1889 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1559 1559
Size: 3647
BsrFI r'CCGG_y
Cuts at: 1645 1826 2035 1645
Size: 181 209 3257
Fragments arranged by size:
3257 209 181
BssSI C'ACGA_G
Cuts at: 1918 2276 1918
Size: 358 3289
BstAPI GCAn_nnn'nTGC
Cuts at: 1024 1024
Size: 3647
BstBI TT'CG_AA
Cuts at: 2008 2008
Size: 3647
BstEII G'GTnAC_C
Cuts at: 334 334
Size: 3647
BstYI r'GATC_y
Cuts at: 1 234 672 1139 1162 1497 1743 2755 2841
Size: 233 438 467 23 335 246 1012 86
Cuts at: 2841 2853 3199 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 438 346 335 246 233
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 463 463
Size: 3647
BtgI C'CryG_G
Cuts at: 521 521
Size: 3647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1597 1778 1941 1597
Size: 181 163 3303
Fragments arranged by size:
3303 181 163
BtsI GCAGTG_nn'
Cuts at: 121 890 121
Size: 769 2878
ClaI AT'CG_AT
Cuts at: 489 489
Size: 3647
DraI TTT'AAA
Cuts at: 646 646
Size: 3647
DrdI GACnn_nn'nnGTC
Cuts at: 1353 2211 1353
Size: 858 2789
EaeI y'GGCC_r
Cuts at: 1232 1406 1797 1824 3169 1232
Size: 174 391 27 1345 1710
Fragments arranged by size:
1710 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1232 1232
Size: 3647
EarI CTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1855 2165 2311 3457 1855
Size: 310 146 1146 2045
Fragments arranged by size:
2045 1146 310 146
EcoICRI GAG'CTC
Cuts at: 659 659
Size: 3647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 340 400 475 1472 1904 2651 2919 234
Size: 106 60 75 997 432 747 268 962
Fragments arranged by size:
997 962 747 432 268 106 75 60
EcoNI CCTnn'n_nnAGG
Cuts at: 382 382
Size: 3647
EcoO109I rG'GnC_Cy
Cuts at: 786 786
Size: 3647
EcoRI G'AATT_C
Cuts at: 651 651
Size: 3647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 285 330 558 1666 285
Size: 45 228 1108 2266
Fragments arranged by size:
2266 1108 228 45
FspI TGC'GCA
Cuts at: 1428 1428
Size: 3647
HaeII r_GCGC'y
Cuts at: 483 1081 1329 2351 3330 483
Size: 598 248 1022 979 800
Fragments arranged by size:
1022 979 800 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1489 1519 1521 1551 1489
Size: 30 2 30 3585
Fragments arranged by size:
3585 30 30 2
HincII GTy'rAC
Cuts at: 686 686
Size: 3647
KasI G'GCGC_C
Cuts at: 1077 1325 1077
Size: 248 3399
KpnI G_GTAC'C
Cuts at: 667 667
Size: 3647
MluI A'CGCG_T
Cuts at: 3080 3080
Size: 3647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2502 2318
Size: 184 3463
MscI TGG'CCA
Cuts at: 1408 1408
Size: 3647
MslI CAynn'nnrTG
Cuts at: 625 1636 1763 625
Size: 1011 127 2509
Fragments arranged by size:
2509 1011 127
MspA1I CmG'CkG
Cuts at: 194 288 758 1072 1432 2445 2690 194
Size: 94 470 314 360 1013 245 1151
Fragments arranged by size:
1151 1013 470 360 314 245 94
NaeI GCC'GGC
Cuts at: 1828 1828
Size: 3647
NarI GG'CG_CC
Cuts at: 1078 1326 1078
Size: 248 3399
NgoMIV G'CCGG_C
Cuts at: 1826 1826
Size: 3647
NspI r_CATG'y
Cuts at: 228 433 700 1200 2107 228
Size: 205 267 500 907 1768
Fragments arranged by size:
1768 907 500 267 205
PasI CC'CwG_GG
Cuts at: 441 441
Size: 3647
PciI A'CATG_T
Cuts at: 2103 2103
Size: 3647
PmeI GTTT'AAAC
Cuts at: 646 646
Size: 3647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2786 2818 2786
Size: 32 3615
PstI C_TGCA'G
Cuts at: 694 1379 694
Size: 685 2962
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3647
PvuII CAG'CTG
Cuts at: 194 288 1072 1432 194
Size: 94 784 360 2409
Fragments arranged by size:
2409 784 360 94
RsrII CG'GwC_CG
Cuts at: 1842 1842
Size: 3647
SacI G_AGCT'C
Cuts at: 661 661
Size: 3647
SalI G'TCGA_C
Cuts at: 684 684
Size: 3647
SapI GCTCTTCn'nnn_
Cuts at: 1670 1880 1670
Size: 210 3437
SbfI CC_TGCA'GG
Cuts at: 694 694
Size: 3647
ScaI AGT'ACT
Cuts at: 3164 3164
Size: 3647
SfcI C'TryA_G
Cuts at: 33 690 1375 2368 2559 33
Size: 657 685 993 191 1121
Fragments arranged by size:
1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 1079 1327 1079
Size: 248 3399
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3647
SmaI CCC'GGG
Cuts at: 669 669
Size: 3647
SmlI C'TyrA_G
Cuts at: 802 1717 2209 2471 802
Size: 915 492 262 1978
Fragments arranged by size:
1978 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3647
SphI G_CATG'C
Cuts at: 700 1200 700
Size: 500 3147
StyI C'CwwG_G
Cuts at: 781 1135 781
Size: 354 3293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 214 1230 1363 1822 2031 193
Size: 21 1016 133 459 209 1809
Fragments arranged by size:
1809 1016 459 209 133 21
TatI w'GTAC_w
Cuts at: 3162 3162
Size: 3647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 3615
Tth111I GACn'n_nGTC
Cuts at: 1444 1444
Size: 3647
XbaI T'CTAG_A
Cuts at: 678 678
Size: 3647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1142 1142
Size: 3647
XmaI C'CCGG_G
Cuts at: 667 667
Size: 3647
XmnI GAAnn'nnTTC
Cuts at: 265 265
Size: 3647
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI