Restriction map for
pF1KB6856
(Circular) (Six-base) MAPSORT of: pF1KB6856.seq Check: 4606 from: 1 to: 3695
pF1KB6856 3695 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 349 381 349
Size: 32 3663
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 481 2465 481
Size: 1984 1711
ApoI r'AATT_y
Cuts at: 404 670 699 3048 3304 404
Size: 266 29 2349 256 795
Fragments arranged by size:
2349 795 266 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 501 501
Size: 3695
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 221 807 221
Size: 586 3109
Bme1580I G_kGCm'C
Cuts at: 485 1320 1413 2469 485
Size: 835 93 1056 1711
Fragments arranged by size:
1711 1056 835 93
BmrI ACTGGGnnnn_n'
Cuts at: 907 988 1323 3467 907
Size: 81 335 2144 1135
Fragments arranged by size:
2144 1135 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 222 485 2062 222
Size: 263 1577 1855
Fragments arranged by size:
1855 1577 263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 217 211
Size: 6 3689
Bpu10I CC'TnA_GC
Cuts at: 226 251 413 226
Size: 25 162 3508
Fragments arranged by size:
3508 162 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 517 871 1750 2242 2540 199
Size: 318 354 879 492 298 1354
Fragments arranged by size:
1354 879 492 354 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 1048 1048
Size: 3695
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 1126 1374 2076 3572 1126
Size: 248 702 1496 1249
Fragments arranged by size:
1496 1249 702 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 349
Size: 30 3665
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 414 414
Size: 3695
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1237 1283 2491 104
Size: 1133 46 1208 1308
Fragments arranged by size:
1308 1208 1133 46
BsiHKAI G_wGCw'C
Cuts at: 485 709 1487 1677 2469 485
Size: 224 778 190 792 1711
Fragments arranged by size:
1711 792 778 224 190
Bsp1286I G_dGCh'C
Cuts at: 485 709 1320 1413 1487 1677 1739 2469 485
Size: 224 611 93 74 190 62 730 1711
Fragments arranged by size:
1711 730 611 224 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 2871 2871
Size: 3695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 745 959 1261 1642 745
Size: 214 302 381 2798
Fragments arranged by size:
2798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 560 1693 1874 2083 560
Size: 1133 181 209 2172
Fragments arranged by size:
2172 1133 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2803 2889 2901
Size: 719 467 23 335 246 1012 86 12
Cuts at: 2901 3247 1
Size: 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 1280 1454 1845 1872 3217 1280
Size: 174 391 27 1345 1758
Fragments arranged by size:
1758 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 191 1718 1928 191
Size: 1527 210 1958
Fragments arranged by size:
1958 1527 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 217 1520 1952 2699 2967 211
Size: 6 1303 432 747 268 939
Fragments arranged by size:
1303 939 747 432 268 6
EcoO109I rG'GnC_Cy
Cuts at: 834 834
Size: 3695
EcoRI G'AATT_C
Cuts at: 404 670 699 404
Size: 266 29 3400
Fragments arranged by size:
3400 266 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 125 1129 1377 2399 3378 125
Size: 1004 248 1022 979 442
Fragments arranged by size:
1022 1004 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 1537 1567 1569 1599 151
Size: 32 1354 30 2 30 2247
Fragments arranged by size:
2247 1354 32 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MfeI C'AATT_G
Cuts at: 685 685
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 256 2366 2550 256
Size: 2110 184 1401
Fragments arranged by size:
2110 1401 184
MscI TGG'CCA
Cuts at: 1456 1456
Size: 3695
MslI CAynn'nnrTG
Cuts at: 1684 1811 1684
Size: 127 3568
MspA1I CmG'CkG
Cuts at: 478 806 1120 1480 2493 2738 478
Size: 328 314 360 1013 245 1435
Fragments arranged by size:
1435 1013 360 328 314 245
NaeI GCC'GGC
Cuts at: 562 1876 562
Size: 1314 2381
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 560 1874 560
Size: 1314 2381
NspI r_CATG'y
Cuts at: 382 748 1248 2155 382
Size: 366 500 907 1922
Fragments arranged by size:
1922 907 500 366
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 349 381 2834 2866 349
Size: 32 2453 32 1178
Fragments arranged by size:
2453 1178 32 32
PstI C_TGCA'G
Cuts at: 742 1427 742
Size: 685 3010
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 478 1120 1480 478
Size: 642 360 2693
Fragments arranged by size:
2693 642 360
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 191 1718 1928 191
Size: 1527 210 1958
Fragments arranged by size:
1958 1527 210
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 738 1423 2416 2607 33
Size: 705 685 993 191 1121
Fragments arranged by size:
1121 993 705 685 191
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 214 496 850 1765 2257 2519 214
Size: 282 354 915 492 262 1390
Fragments arranged by size:
1390 915 492 354 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
SspI AAT'ATT
Cuts at: 240 240
Size: 3695
StyI C'CwwG_G
Cuts at: 829 1183 829
Size: 354 3341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1278 1411 1870 2079 1278
Size: 133 459 209 2894
Fragments arranged by size:
2894 459 209 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 505 1492 505
Size: 987 2708
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
XmnI GAAnn'nnTTC
Cuts at: 319 364 319
Size: 45 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI