Restriction map for
pF1KB6859
(Circular) (Six-base) MAPSORT of: pF1KB6859.seq Check: 7320 from: 1 to: 3650
pF1KB6859 3650 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1907 2654 2922 1475
Size: 432 747 268 2203
Fragments arranged by size:
2203 747 432 268
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 2038 2038
Size: 3650
AlwNI CAG_nnn'CTG
Cuts at: 262 955 2522 262
Size: 693 1567 1390
Fragments arranged by size:
1567 1390 693
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 654 3003 3259 654
Size: 2349 256 1045
Fragments arranged by size:
2349 1045 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 670 670
Size: 3650
AvrII C'CTAG_G
Cuts at: 582 582
Size: 3650
BamHI G'GATC_C
Cuts at: 675 675
Size: 3650
BanI G'GyrC_C
Cuts at: 666 1080 1328 1363 666
Size: 414 248 35 2953
Fragments arranged by size:
2953 414 248 35
BanII G_rGCy'C
Cuts at: 664 1694 664
Size: 1030 2620
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3616
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 1275 1368 2424 1275
Size: 93 1056 2501
Fragments arranged by size:
2501 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 143 354 862 943 1278 3422 143
Size: 211 508 81 335 2144 371
Fragments arranged by size:
2144 508 371 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 219
Size: 3650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 1003 1003
Size: 3650
BsaBI GATnn'nnATC
Cuts at: 6 1164 3201 6
Size: 1158 2037 455
Fragments arranged by size:
2037 1158 455
BsaHI Gr'CG_yC
Cuts at: 1081 1329 2031 3527 1081
Size: 248 702 1496 1204
Fragments arranged by size:
1496 1204 702 248
BsaWI w'CCGG_w
Cuts at: 839 1360 2038 2312 2459 2870 839
Size: 521 678 274 147 411 1619
Fragments arranged by size:
1619 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 513 1075 1833 2410 2906 513
Size: 562 758 577 496 1257
Fragments arranged by size:
1257 758 577 562 496
BsiEI CG_ry'CG
Cuts at: 104 1192 1238 2446 104
Size: 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46
BsiHKAI G_wGCw'C
Cuts at: 367 664 1442 1632 2424 367
Size: 297 778 190 792 1593
Fragments arranged by size:
1593 792 778 297 190
Bsp1286I G_dGCh'C
Cuts at: 367 664 1275 1368 1442 1632 1694 2424 367
Size: 297 611 93 74 190 62 730 1593
Fragments arranged by size:
1593 730 611 297 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2870 2870
Size: 3650
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 1648 1829 2038 1648
Size: 181 209 3260
Fragments arranged by size:
3260 209 181
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 321 2011 321
Size: 1690 1960
BstEII G'GTnAC_C
Cuts at: 628 628
Size: 3650
BstXI CCAn_nnnn'nTGG
Cuts at: 123 456 123
Size: 333 3317
BstYI r'GATC_y
Cuts at: 1 675 1142 1165 1500 1746 2758 2844 2856
Size: 674 467 23 335 246 1012 86 12
Cuts at: 2856 3202 1
Size: 346 449
Fragments arranged by size:
1012 674 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 893 893
Size: 3650
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 441 1235 1409 1800 1827 3172 441
Size: 794 174 391 27 1345 919
Fragments arranged by size:
1345 919 794 391 174 27
EagI C'GGCC_G
Cuts at: 1235 1235
Size: 3650
EarI CTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 1475 1907 2654 2922 219
Size: 1256 432 747 268 947
Fragments arranged by size:
1256 947 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 789 789
Size: 3650
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 1084 1332 2354 3333 1084
Size: 248 1022 979 1401
Fragments arranged by size:
1401 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 2321 2505 150
Size: 2171 184 1295
Fragments arranged by size:
2171 1295 184
MscI TGG'CCA
Cuts at: 443 1411 443
Size: 968 2682
MslI CAynn'nnrTG
Cuts at: 1639 1766 1639
Size: 127 3523
MspA1I CmG'CkG
Cuts at: 293 761 1075 1435 2448 2693 293
Size: 468 314 360 1013 245 1250
Fragments arranged by size:
1250 1013 468 360 314 245
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 189 703 1203 2110 189
Size: 514 500 907 1729
Fragments arranged by size:
1729 907 514 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PstI C_TGCA'G
Cuts at: 697 1382 697
Size: 685 2965
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 1075 1435 1075
Size: 360 3290
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 693 1378 2371 2562 33
Size: 660 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 191
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 672 672
Size: 3650
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
SspI AAT'ATT
Cuts at: 136 136
Size: 3650
StyI C'CwwG_G
Cuts at: 122 449 582 784 1138 122
Size: 327 133 202 354 2634
Fragments arranged by size:
2634 354 327 202 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 1366 1825 2034 1233
Size: 133 459 209 2849
Fragments arranged by size:
2849 459 209 133
TatI w'GTAC_w
Cuts at: 151 299 3165 151
Size: 148 2866 636
Fragments arranged by size:
2866 636 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3618
Tth111I GACn'n_nGTC
Cuts at: 619 1447 619
Size: 828 2822
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 670 670
Size: 3650
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI