Restriction map for
pF1KB6860
(Circular) (Six-base) MAPSORT of: pF1KB6860.seq Check: 8037 from: 1 to: 3674
pF1KB6860 3674 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 712 712
Size: 3674
Acc65I G'GTAC_C
Cuts at: 690 690
Size: 3674
AclI AA'CG_TT
Cuts at: 3514 3514
Size: 3674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1499 1931 2678 2946 513
Size: 986 432 747 268 1241
Fragments arranged by size:
1241 986 747 432 268
AfeI AGC'GCT
Cuts at: 286 286
Size: 3674
AflIII A'CryG_T
Cuts at: 1026 2130 3107 1026
Size: 1104 977 1593
Fragments arranged by size:
1593 1104 977
AgeI A'CCGG_T
Cuts at: 2062 2062
Size: 3674
AleI CACnn'nnGTG
Cuts at: 310 310
Size: 3674
AlwNI CAG_nnn'CTG
Cuts at: 369 979 2546 369
Size: 610 1567 1497
Fragments arranged by size:
1567 1497 610
ApaI G_GGCC'C
Cuts at: 165 172 206 585 165
Size: 7 34 379 3254
Fragments arranged by size:
3254 379 34 7
ApaLI G'TGCA_C
Cuts at: 2444 2444
Size: 3674
ApoI r'AATT_y
Cuts at: 678 3027 3283 678
Size: 2349 256 1069
Fragments arranged by size:
2349 1069 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
AvaI C'yCGr_G
Cuts at: 165 171 195 215 694 165
Size: 6 24 20 479 3145
Fragments arranged by size:
3145 479 24 20 6
BamHI G'GATC_C
Cuts at: 699 699
Size: 3674
BanI G'GyrC_C
Cuts at: 333 690 1104 1352 1387 333
Size: 357 414 248 35 2620
Fragments arranged by size:
2620 414 357 248 35
BanII G_rGCy'C
Cuts at: 165 172 206 415 585 688 1718 165
Size: 7 34 209 170 103 1030 2121
Fragments arranged by size:
2121 1030 209 170 103 34 7
BbeI G_GCGC'C
Cuts at: 1108 1356 1108
Size: 248 3426
BbvCI CC'TCA_GC
Cuts at: 497 497
Size: 3674
BciVI GTATCCnnnnn_n'
Cuts at: 1572 2333 3674 1572
Size: 761 1341 1572
Fragments arranged by size:
1572 1341 761
BclI T'GATC_A
Cuts at: 1194 1194
Size: 3674
BglI GCCn_nnn'nGGC
Cuts at: 469 469
Size: 3674
BglII A'GATC_T
Cuts at: 1189 3226 1189
Size: 2037 1637
BlpI GC'TnA_GC
Cuts at: 786 786
Size: 3674
Bme1580I G_kGCm'C
Cuts at: 165 172 206 585 1299 1392 2448 165
Size: 7 34 379 714 93 1056 1391
Fragments arranged by size:
1391 1056 714 379 93 34 7
BmgBI CAC'GTC
Cuts at: 276 276
Size: 3674
BmrI ACTGGGnnnn_n'
Cuts at: 886 967 1302 3446 886
Size: 81 335 2144 1114
Fragments arranged by size:
2144 1114 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 3674
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 3674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 850 1729 2221 2519 450
Size: 400 879 492 298 1605
Fragments arranged by size:
1605 879 492 400 298
BsaI GGTCTCn'nnnn_
Cuts at: 3378 3378
Size: 3674
BsaAI yAC'GTr
Cuts at: 1027 1027
Size: 3674
BsaBI GATnn'nnATC
Cuts at: 6 1188 3225 6
Size: 1182 2037 455
Fragments arranged by size:
2037 1182 455
BsaHI Gr'CG_yC
Cuts at: 1105 1353 2055 3551 1105
Size: 248 702 1496 1228
Fragments arranged by size:
1496 1228 702 248
BsaWI w'CCGG_w
Cuts at: 863 1384 2062 2336 2483 2894 863
Size: 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 584 629 663 1099 1857 2434 2930 584
Size: 45 34 436 758 577 496 1328
Fragments arranged by size:
1328 758 577 496 436 45 34
BsiEI CG_ry'CG
Cuts at: 104 1216 1262 2470 104
Size: 1112 46 1208 1308
Fragments arranged by size:
1308 1208 1112 46
BsiHKAI G_wGCw'C
Cuts at: 359 415 688 1466 1656 2448 359
Size: 56 273 778 190 792 1585
Fragments arranged by size:
1585 792 778 273 190 56
Bsp1286I G_dGCh'C
Cuts at: 165 172 206 359 415 585 688 1299 1392
Size: 7 34 153 56 170 103 611 93
Cuts at: 1392 1466 1656 1718 2448 165
Size: 74 190 62 730 1391
Fragments arranged by size:
1391 730 611 190 170 153 103 93
74 62 56 34 7
BspEI T'CCGG_A
Cuts at: 2894 2894
Size: 3674
BspHI T'CATG_A
Cuts at: 2850 2850
Size: 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 377 724 938 1240 1621 377
Size: 347 214 302 381 2430
Fragments arranged by size:
2430 381 347 302 214
BsrBI CCG'CTC
Cuts at: 50 222 1966 2020 3504 3669 50
Size: 172 1744 54 1484 165 55
Fragments arranged by size:
1744 1484 172 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 1586
Size: 3674
BsrFI r'CCGG_y
Cuts at: 1672 1853 2062 1672
Size: 181 209 3284
Fragments arranged by size:
3284 209 181
BssSI C'ACGA_G
Cuts at: 1945 2303 1945
Size: 358 3316
BstAPI GCAn_nnn'nTGC
Cuts at: 1051 1051
Size: 3674
BstBI TT'CG_AA
Cuts at: 2035 2035
Size: 3674
BstYI r'GATC_y
Cuts at: 1 593 699 1166 1189 1524 1770 2782 2868
Size: 592 106 467 23 335 246 1012 86
Cuts at: 2868 2880 3226 1
Size: 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 246 106
86 23 12
BtgI C'CryG_G
Cuts at: 107 157 189 431 572 107
Size: 50 32 242 141 3209
Fragments arranged by size:
3209 242 141 50 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1624 1805 1968 1624
Size: 181 163 3330
Fragments arranged by size:
3330 181 163
BtsI GCAGTG_nn'
Cuts at: 621 917 621
Size: 296 3378
DraI TTT'AAA
Cuts at: 673 673
Size: 3674
DrdI GACnn_nn'nnGTC
Cuts at: 532 1380 2238 532
Size: 848 858 1968
Fragments arranged by size:
1968 858 848
EaeI y'GGCC_r
Cuts at: 428 1259 1433 1824 1851 3196 428
Size: 831 174 391 27 1345 906
Fragments arranged by size:
1345 906 831 391 174 27
EagI C'GGCC_G
Cuts at: 1259 1259
Size: 3674
EarI CTCTTCn'nnn_
Cuts at: 124 1697 1907 124
Size: 1573 210 1891
Fragments arranged by size:
1891 1573 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1882 2192 2338 3484 1882
Size: 310 146 1146 2072
Fragments arranged by size:
2072 1146 310 146
EcoICRI GAG'CTC
Cuts at: 413 686 413
Size: 273 3401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 1499 1931 2678 2946 513
Size: 986 432 747 268 1241
Fragments arranged by size:
1241 986 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 162 202 203 366 581 813 162
Size: 40 1 163 215 232 3023
Fragments arranged by size:
3023 232 215 163 40 1
EcoRI G'AATT_C
Cuts at: 678 678
Size: 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1693 1693
Size: 3674
FspI TGC'GCA
Cuts at: 1455 1455
Size: 3674
HaeII r_GCGC'y
Cuts at: 129 288 1108 1356 2378 3357 129
Size: 159 820 248 1022 979 446
Fragments arranged by size:
1022 979 820 446 248 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1516 1546 1548 1578 1516
Size: 30 2 30 3612
Fragments arranged by size:
3612 30 30 2
HincII GTy'rAC
Cuts at: 713 713
Size: 3674
KasI G'GCGC_C
Cuts at: 1104 1352 1104
Size: 248 3426
KpnI G_GTAC'C
Cuts at: 694 694
Size: 3674
MluI A'CGCG_T
Cuts at: 3107 3107
Size: 3674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2345 2529 2345
Size: 184 3490
MscI TGG'CCA
Cuts at: 430 1435 430
Size: 1005 2669
MslI CAynn'nnrTG
Cuts at: 310 1663 1790 310
Size: 1353 127 2194
Fragments arranged by size:
2194 1353 127
MspA1I CmG'CkG
Cuts at: 159 588 600 785 1099 1459 2472 2717 159
Size: 429 12 185 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 429 360 314 245 185 12
NaeI GCC'GGC
Cuts at: 1855 1855
Size: 3674
NarI GG'CG_CC
Cuts at: 1105 1353 1105
Size: 248 3426
NcoI C'CATG_G
Cuts at: 107 431 107
Size: 324 3350
NgoMIV G'CCGG_C
Cuts at: 1853 1853
Size: 3674
NspI r_CATG'y
Cuts at: 727 1227 2134 727
Size: 500 907 2267
Fragments arranged by size:
2267 907 500
PciI A'CATG_T
Cuts at: 2130 2130
Size: 3674
PflMI CCAn_nnn'nTGG
Cuts at: 437 437
Size: 3674
PfoI T'CCnGG_A
Cuts at: 208 208
Size: 3674
PmeI GTTT'AAAC
Cuts at: 673 673
Size: 3674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3642
PpuMI rG'GwC_Cy
Cuts at: 366 366
Size: 3674
PspOMI G'GGCC_C
Cuts at: 161 168 202 581 161
Size: 7 34 379 3254
Fragments arranged by size:
3254 379 34 7
PstI C_TGCA'G
Cuts at: 332 721 1406 332
Size: 389 685 2600
Fragments arranged by size:
2600 685 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3674
PvuII CAG'CTG
Cuts at: 600 1099 1459 600
Size: 499 360 2815
Fragments arranged by size:
2815 499 360
RsrII CG'GwC_CG
Cuts at: 1869 1869
Size: 3674
SacI G_AGCT'C
Cuts at: 415 688 415
Size: 273 3401
SacII CC_GC'GG
Cuts at: 160 160
Size: 3674
SalI G'TCGA_C
Cuts at: 711 711
Size: 3674
SapI GCTCTTCn'nnn_
Cuts at: 1697 1907 1697
Size: 210 3464
SbfI CC_TGCA'GG
Cuts at: 721 721
Size: 3674
ScaI AGT'ACT
Cuts at: 3191 3191
Size: 3674
SfcI C'TryA_G
Cuts at: 33 328 717 1402 2395 2586 33
Size: 295 389 685 993 191 1121
Fragments arranged by size:
1121 993 685 389 295 191
SfoI GGC'GCC
Cuts at: 1106 1354 1106
Size: 248 3426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3674
SmaI CCC'GGG
Cuts at: 167 217 696 167
Size: 50 479 3145
Fragments arranged by size:
3145 479 50
SmlI C'TyrA_G
Cuts at: 465 829 1744 2236 2498 465
Size: 364 915 492 262 1641
Fragments arranged by size:
1641 915 492 364 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3674
SphI G_CATG'C
Cuts at: 727 1227 727
Size: 500 3174
StyI C'CwwG_G
Cuts at: 107 431 808 1162 107
Size: 324 377 354 2619
Fragments arranged by size:
2619 377 354 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 677 1257 1390 1849 2058 677
Size: 580 133 459 209 2293
Fragments arranged by size:
2293 580 459 209 133
TatI w'GTAC_w
Cuts at: 3189 3189
Size: 3674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 3642
Tth111I GACn'n_nGTC
Cuts at: 1471 1471
Size: 3674
XbaI T'CTAG_A
Cuts at: 705 705
Size: 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 570 1169 570
Size: 599 3075
XmaI C'CCGG_G
Cuts at: 165 215 694 165
Size: 50 479 3145
Fragments arranged by size:
3145 479 50
XmnI GAAnn'nnTTC
Cuts at: 382 382
Size: 3674
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BpmI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI