Restriction map for
pF1KB6864
(Circular) (Six-base) MAPSORT of: pF1KB6864.seq Check: 4548 from: 1 to: 3698
pF1KB6864 3698 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 736 736
Size: 3698
Acc65I G'GTAC_C
Cuts at: 714 714
Size: 3698
AclI AA'CG_TT
Cuts at: 3538 3538
Size: 3698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
AflII C'TTAA_G
Cuts at: 391 391
Size: 3698
AflIII A'CryG_T
Cuts at: 1050 2154 3131 1050
Size: 1104 977 1617
Fragments arranged by size:
1617 1104 977
AgeI A'CCGG_T
Cuts at: 2086 2086
Size: 3698
AlwNI CAG_nnn'CTG
Cuts at: 297 1003 2570 297
Size: 706 1567 1425
Fragments arranged by size:
1567 1425 706
ApaLI G'TGCA_C
Cuts at: 2468 2468
Size: 3698
ApoI r'AATT_y
Cuts at: 458 649 679 702 3051 3307 458
Size: 191 30 23 2349 256 849
Fragments arranged by size:
2349 849 256 191 30 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
AvaI C'yCGr_G
Cuts at: 171 665 718 171
Size: 494 53 3151
Fragments arranged by size:
3151 494 53
BamHI G'GATC_C
Cuts at: 723 723
Size: 3698
BanI G'GyrC_C
Cuts at: 111 181 714 1128 1376 1411 111
Size: 70 533 414 248 35 2398
Fragments arranged by size:
2398 533 414 248 70 35
BanII G_rGCy'C
Cuts at: 712 1742 712
Size: 1030 2668
BbeI G_GCGC'C
Cuts at: 115 1132 1380 115
Size: 1017 248 2433
Fragments arranged by size:
2433 1017 248
BciVI GTATCCnnnnn_n'
Cuts at: 601 1596 2357 3698 601
Size: 995 761 1341 601
Fragments arranged by size:
1341 995 761 601
BclI T'GATC_A
Cuts at: 1218 1218
Size: 3698
BglII A'GATC_T
Cuts at: 1213 3250 1213
Size: 2037 1661
BlpI GC'TnA_GC
Cuts at: 810 810
Size: 3698
Bme1580I G_kGCm'C
Cuts at: 631 1323 1416 2472 631
Size: 692 93 1056 1857
Fragments arranged by size:
1857 1056 692 93
BmrI ACTGGGnnnn_n'
Cuts at: 285 910 991 1326 3470 285
Size: 625 81 335 2144 513
Fragments arranged by size:
2144 625 513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 3698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1753 2245 2543 874
Size: 879 492 298 2029
Fragments arranged by size:
2029 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 336 3402 336
Size: 3066 632
BsaAI yAC'GTr
Cuts at: 1051 1051
Size: 3698
BsaBI GATnn'nnATC
Cuts at: 6 1212 3249 6
Size: 1206 2037 455
Fragments arranged by size:
2037 1206 455
BsaHI Gr'CG_yC
Cuts at: 112 1129 1377 2079 3575 112
Size: 1017 248 702 1496 235
Fragments arranged by size:
1496 1017 702 248 235
BsaWI w'CCGG_w
Cuts at: 653 887 1408 2086 2360 2507 2918 653
Size: 234 521 678 274 147 411 1433
Fragments arranged by size:
1433 678 521 411 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 302 332 554 584 302
Size: 30 222 30 3416
Fragments arranged by size:
3416 222 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 3698
BseYI C'CCAG_C
Cuts at: 558 1123 1881 2458 2954 558
Size: 565 758 577 496 1302
Fragments arranged by size:
1302 758 577 565 496
BsiEI CG_ry'CG
Cuts at: 104 1240 1286 2494 104
Size: 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 46
BsiHKAI G_wGCw'C
Cuts at: 712 1490 1680 2472 712
Size: 778 190 792 1938
Fragments arranged by size:
1938 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 631 712 1323 1416 1490 1680 1742 2472 631
Size: 81 611 93 74 190 62 730 1857
Fragments arranged by size:
1857 730 611 190 93 81 74 62
BspEI T'CCGG_A
Cuts at: 653 2918 653
Size: 2265 1433
BspHI T'CATG_A
Cuts at: 2874 2874
Size: 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 542 748 962 1264 1645 542
Size: 206 214 302 381 2595
Fragments arranged by size:
2595 381 302 214 206
BsrBI CCG'CTC
Cuts at: 50 1990 2044 3528 3693 50
Size: 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1610 1610
Size: 3698
BsrFI r'CCGG_y
Cuts at: 220 1696 1877 2086 220
Size: 1476 181 209 1832
Fragments arranged by size:
1832 1476 209 181
BssHII G'CGCG_C
Cuts at: 251 251
Size: 3698
BssSI C'ACGA_G
Cuts at: 1969 2327 1969
Size: 358 3340
BstAPI GCAn_nnn'nTGC
Cuts at: 1075 1075
Size: 3698
BstBI TT'CG_AA
Cuts at: 2059 2059
Size: 3698
BstYI r'GATC_y
Cuts at: 1 723 1190 1213 1548 1794 2806 2892 2904
Size: 722 467 23 335 246 1012 86 12
Cuts at: 2904 3250 1
Size: 346 449
Fragments arranged by size:
1012 722 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 510 510
Size: 3698
BtgI C'CryG_G
Cuts at: 107 195 107
Size: 88 3610
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1648 1829 1992 1648
Size: 181 163 3354
Fragments arranged by size:
3354 181 163
BtsI GCAGTG_nn'
Cuts at: 631 941 631
Size: 310 3388
DraI TTT'AAA
Cuts at: 697 697
Size: 3698
DrdI GACnn_nn'nnGTC
Cuts at: 1404 2262 1404
Size: 858 2840
EaeI y'GGCC_r
Cuts at: 494 1283 1457 1848 1875 3220 494
Size: 789 174 391 27 1345 972
Fragments arranged by size:
1345 972 789 391 174 27
EagI C'GGCC_G
Cuts at: 1283 1283
Size: 3698
EarI CTCTTCn'nnn_
Cuts at: 229 1721 1931 229
Size: 1492 210 1996
Fragments arranged by size:
1996 1492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 463 1906 2216 2362 3508 463
Size: 1443 310 146 1146 653
Fragments arranged by size:
1443 1146 653 310 146
EcoICRI GAG'CTC
Cuts at: 710 710
Size: 3698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1955 2702 2970 1523
Size: 432 747 268 2251
Fragments arranged by size:
2251 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 837 837
Size: 3698
EcoRI G'AATT_C
Cuts at: 649 679 702 649
Size: 30 23 3645
Fragments arranged by size:
3645 30 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1717 1717
Size: 3698
FspI TGC'GCA
Cuts at: 1479 1479
Size: 3698
HaeII r_GCGC'y
Cuts at: 115 1132 1380 2402 3381 115
Size: 1017 248 1022 979 432
Fragments arranged by size:
1022 1017 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 398 430 508 540 1540 1570 1572
Size: 32 250 32 78 32 1000 30 2
Cuts at: 1572 1602 116
Size: 30 2212
Fragments arranged by size:
2212 1000 250 78 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 737 737
Size: 3698
KasI G'GCGC_C
Cuts at: 111 1128 1376 111
Size: 1017 248 2433
Fragments arranged by size:
2433 1017 248
KpnI G_GTAC'C
Cuts at: 718 718
Size: 3698
MluI A'CGCG_T
Cuts at: 3131 3131
Size: 3698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 551 2369 2553 551
Size: 1818 184 1696
Fragments arranged by size:
1818 1696 184
MscI TGG'CCA
Cuts at: 496 1459 496
Size: 963 2735
MslI CAynn'nnrTG
Cuts at: 1687 1814 1687
Size: 127 3571
MspA1I CmG'CkG
Cuts at: 179 611 809 1123 1483 2496 2741 179
Size: 432 198 314 360 1013 245 1136
Fragments arranged by size:
1136 1013 432 360 314 245 198
NaeI GCC'GGC
Cuts at: 222 1879 222
Size: 1657 2041
NarI GG'CG_CC
Cuts at: 112 1129 1377 112
Size: 1017 248 2433
Fragments arranged by size:
2433 1017 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3698
NgoMIV G'CCGG_C
Cuts at: 220 1877 220
Size: 1657 2041
NspI r_CATG'y
Cuts at: 751 1251 2158 751
Size: 500 907 2291
Fragments arranged by size:
2291 907 500
PciI A'CATG_T
Cuts at: 2154 2154
Size: 3698
PflMI CCAn_nnn'nTGG
Cuts at: 611 611
Size: 3698
PmeI GTTT'AAAC
Cuts at: 697 697
Size: 3698
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 3666
PsiI TTA'TAA
Cuts at: 464 464
Size: 3698
PstI C_TGCA'G
Cuts at: 745 1430 745
Size: 685 3013
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3698
PvuII CAG'CTG
Cuts at: 611 1123 1483 611
Size: 512 360 2826
Fragments arranged by size:
2826 512 360
RsrII CG'GwC_CG
Cuts at: 1893 1893
Size: 3698
SacI G_AGCT'C
Cuts at: 712 712
Size: 3698
SalI G'TCGA_C
Cuts at: 735 735
Size: 3698
SapI GCTCTTCn'nnn_
Cuts at: 229 1721 1931 229
Size: 1492 210 1996
Fragments arranged by size:
1996 1492 210
SbfI CC_TGCA'GG
Cuts at: 745 745
Size: 3698
ScaI AGT'ACT
Cuts at: 3215 3215
Size: 3698
SfcI C'TryA_G
Cuts at: 33 269 741 1426 2419 2610 33
Size: 236 472 685 993 191 1121
Fragments arranged by size:
1121 993 685 472 236 191
SfoI GGC'GCC
Cuts at: 113 1130 1378 113
Size: 1017 248 2433
Fragments arranged by size:
2433 1017 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3698
SmaI CCC'GGG
Cuts at: 173 720 173
Size: 547 3151
SmlI C'TyrA_G
Cuts at: 391 853 1768 2260 2522 391
Size: 462 915 492 262 1567
Fragments arranged by size:
1567 915 492 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3698
SphI G_CATG'C
Cuts at: 751 1251 751
Size: 500 3198
SrfI GCCC'GGGC
Cuts at: 173 173
Size: 3698
StyI C'CwwG_G
Cuts at: 107 832 1186 107
Size: 725 354 2619
Fragments arranged by size:
2619 725 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 1414 1873 2082 1281
Size: 133 459 209 2897
Fragments arranged by size:
2897 459 209 133
TatI w'GTAC_w
Cuts at: 360 669 3213 360
Size: 309 2544 845
Fragments arranged by size:
2544 845 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 3666
Tth111I GACn'n_nGTC
Cuts at: 487 1495 487
Size: 1008 2690
XbaI T'CTAG_A
Cuts at: 729 729
Size: 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1193 1193
Size: 3698
XmaI C'CCGG_G
Cuts at: 171 718 171
Size: 547 3151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SspI StuI SwaI XhoI XmnI ZraI