Restriction map for
pF1KB6865
(Circular) (Six-base) MAPSORT of: pF1KB6865.seq Check: 1030 from: 1 to: 3650
pF1KB6865 3650 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 688 688
Size: 3650
Acc65I G'GTAC_C
Cuts at: 666 666
Size: 3650
AclI AA'CG_TT
Cuts at: 3490 3490
Size: 3650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1907 2654 2922 1475
Size: 432 747 268 2203
Fragments arranged by size:
2203 747 432 268
AflIII A'CryG_T
Cuts at: 1002 2106 3083 1002
Size: 1104 977 1569
Fragments arranged by size:
1569 1104 977
AgeI A'CCGG_T
Cuts at: 564 2038 564
Size: 1474 2176
AlwNI CAG_nnn'CTG
Cuts at: 955 2522 955
Size: 1567 2083
ApaLI G'TGCA_C
Cuts at: 2420 2420
Size: 3650
ApoI r'AATT_y
Cuts at: 625 654 3003 3259 625
Size: 29 2349 256 1016
Fragments arranged by size:
2349 1016 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 3650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
AvaI C'yCGr_G
Cuts at: 138 208 670 138
Size: 70 462 3118
Fragments arranged by size:
3118 462 70
BamHI G'GATC_C
Cuts at: 449 675 449
Size: 226 3424
BanI G'GyrC_C
Cuts at: 666 1080 1328 1363 666
Size: 414 248 35 2953
Fragments arranged by size:
2953 414 248 35
BanII G_rGCy'C
Cuts at: 664 1694 664
Size: 1030 2620
BbeI G_GCGC'C
Cuts at: 1084 1332 1084
Size: 248 3402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 525 559 525
Size: 34 3616
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2309 3650 1548
Size: 761 1341 1548
Fragments arranged by size:
1548 1341 761
BclI T'GATC_A
Cuts at: 1170 1170
Size: 3650
BglI GCCn_nnn'nGGC
Cuts at: 185 185
Size: 3650
BglII A'GATC_T
Cuts at: 1165 3202 1165
Size: 2037 1613
BlpI GC'TnA_GC
Cuts at: 762 762
Size: 3650
Bme1580I G_kGCm'C
Cuts at: 1275 1368 2424 1275
Size: 93 1056 2501
Fragments arranged by size:
2501 1056 93
BmgBI CAC'GTC
Cuts at: 426 426
Size: 3650
BmrI ACTGGGnnnn_n'
Cuts at: 175 862 943 1278 3422 175
Size: 687 81 335 2144 403
Fragments arranged by size:
2144 687 403 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 211
Size: 3650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 826 1705 2197 2495 826
Size: 879 492 298 1981
Fragments arranged by size:
1981 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3354 3354
Size: 3650
BsaAI yAC'GTr
Cuts at: 621 1003 621
Size: 382 3268
BsaBI GATnn'nnATC
Cuts at: 6 301 454 1164 3201 6
Size: 295 153 710 2037 455
Fragments arranged by size:
2037 710 455 295 153
BsaHI Gr'CG_yC
Cuts at: 134 1081 1329 2031 3527 134
Size: 947 248 702 1496 257
Fragments arranged by size:
1496 947 702 257 248
BsaWI w'CCGG_w
Cuts at: 452 564 839 1360 2038 2312 2459 2870 452
Size: 112 275 521 678 274 147 411 1232
Fragments arranged by size:
1232 678 521 411 275 274 147 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 464 494 551 581 430
Size: 30 4 30 57 30 3499
Fragments arranged by size:
3499 57 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 379
Size: 3650
BseYI C'CCAG_C
Cuts at: 1075 1833 2410 2906 1075
Size: 758 577 496 1819
Fragments arranged by size:
1819 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 133
Size: 3650
BsiEI CG_ry'CG
Cuts at: 104 177 1192 1238 2446 104
Size: 73 1015 46 1208 1308
Fragments arranged by size:
1308 1208 1015 73 46
BsiHKAI G_wGCw'C
Cuts at: 218 664 1442 1632 2424 218
Size: 446 778 190 792 1444
Fragments arranged by size:
1444 792 778 446 190
Bsp1286I G_dGCh'C
Cuts at: 218 664 1275 1368 1442 1632 1694 2424 218
Size: 446 611 93 74 190 62 730 1444
Fragments arranged by size:
1444 730 611 446 190 93 74 62
BspEI T'CCGG_A
Cuts at: 452 2870 452
Size: 2418 1232
BspHI T'CATG_A
Cuts at: 2826 2826
Size: 3650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 700 914 1216 1597 700
Size: 214 302 381 2753
Fragments arranged by size:
2753 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1942 1996 3480 3645 50
Size: 1892 54 1484 165 55
Fragments arranged by size:
1892 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1562 1562
Size: 3650
BsrFI r'CCGG_y
Cuts at: 564 584 1648 1829 2038 564
Size: 20 1064 181 209 2176
Fragments arranged by size:
2176 1064 209 181 20
BssSI C'ACGA_G
Cuts at: 1921 2279 1921
Size: 358 3292
BstAPI GCAn_nnn'nTGC
Cuts at: 1027 1027
Size: 3650
BstBI TT'CG_AA
Cuts at: 2011 2011
Size: 3650
BstYI r'GATC_y
Cuts at: 1 269 389 449 455 497 675 1142 1165
Size: 268 120 60 6 42 178 467 23
Cuts at: 1165 1500 1746 2758 2844 2856 3202 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 268 246 178
120 86 60 42 23 12 6
BtgI C'CryG_G
Cuts at: 107 148 491 609 107
Size: 41 343 118 3148
Fragments arranged by size:
3148 343 118 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1600 1781 1944 1600
Size: 181 163 3306
Fragments arranged by size:
3306 181 163
BtsI GCAGTG_nn'
Cuts at: 417 893 417
Size: 476 3174
DraI TTT'AAA
Cuts at: 649 649
Size: 3650
DrdI GACnn_nn'nnGTC
Cuts at: 1356 2214 1356
Size: 858 2792
EaeI y'GGCC_r
Cuts at: 174 1235 1409 1800 1827 3172 174
Size: 1061 174 391 27 1345 652
Fragments arranged by size:
1345 1061 652 391 174 27
EagI C'GGCC_G
Cuts at: 174 1235 174
Size: 1061 2589
EarI CTCTTCn'nnn_
Cuts at: 270 1673 1883 270
Size: 1403 210 2037
Fragments arranged by size:
2037 1403 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1858 2168 2314 3460 1858
Size: 310 146 1146 2048
Fragments arranged by size:
2048 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 662
Size: 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 1475 1907 2654 2922 211
Size: 1264 432 747 268 939
Fragments arranged by size:
1264 939 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 789 789
Size: 3650
EcoRI G'AATT_C
Cuts at: 654 654
Size: 3650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1669 1669
Size: 3650
FspI TGC'GCA
Cuts at: 1431 1431
Size: 3650
HaeII r_GCGC'y
Cuts at: 1084 1332 2354 3333 1084
Size: 248 1022 979 1401
Fragments arranged by size:
1401 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1492 1522 1524 1554 1492
Size: 30 2 30 3588
Fragments arranged by size:
3588 30 30 2
HincII GTy'rAC
Cuts at: 689 689
Size: 3650
KasI G'GCGC_C
Cuts at: 1080 1328 1080
Size: 248 3402
KpnI G_GTAC'C
Cuts at: 670 670
Size: 3650
MluI A'CGCG_T
Cuts at: 3083 3083
Size: 3650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2505 2321
Size: 184 3466
MscI TGG'CCA
Cuts at: 1411 1411
Size: 3650
MslI CAynn'nnrTG
Cuts at: 1639 1766 1639
Size: 127 3523
MspA1I CmG'CkG
Cuts at: 150 198 493 592 611 761 1075 1435 2448
Size: 48 295 99 19 150 314 360 1013
Cuts at: 2448 2693 150
Size: 245 1107
Fragments arranged by size:
1107 1013 360 314 295 245 150 99
48 19
NaeI GCC'GGC
Cuts at: 1831 1831
Size: 3650
NarI GG'CG_CC
Cuts at: 1081 1329 1081
Size: 248 3402
NcoI C'CATG_G
Cuts at: 107 107
Size: 3650
NdeI CA'TA_TG
Cuts at: 313 313
Size: 3650
NgoMIV G'CCGG_C
Cuts at: 1829 1829
Size: 3650
NspI r_CATG'y
Cuts at: 703 1203 2110 703
Size: 500 907 2243
Fragments arranged by size:
2243 907 500
PciI A'CATG_T
Cuts at: 2106 2106
Size: 3650
PfoI T'CCnGG_A
Cuts at: 292 401 292
Size: 109 3541
PmeI GTTT'AAAC
Cuts at: 649 649
Size: 3650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2789 2821 2789
Size: 32 3618
PstI C_TGCA'G
Cuts at: 697 1382 697
Size: 685 2965
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3650
PvuII CAG'CTG
Cuts at: 198 1075 1435 198
Size: 877 360 2413
Fragments arranged by size:
2413 877 360
RsrII CG'GwC_CG
Cuts at: 1845 1845
Size: 3650
SacI G_AGCT'C
Cuts at: 664 664
Size: 3650
SacII CC_GC'GG
Cuts at: 151 494 612 151
Size: 343 118 3189
Fragments arranged by size:
3189 343 118
SalI G'TCGA_C
Cuts at: 687 687
Size: 3650
SapI GCTCTTCn'nnn_
Cuts at: 1673 1883 1673
Size: 210 3440
SbfI CC_TGCA'GG
Cuts at: 697 697
Size: 3650
ScaI AGT'ACT
Cuts at: 3167 3167
Size: 3650
SfcI C'TryA_G
Cuts at: 33 693 1378 2371 2562 33
Size: 660 685 993 191 1121
Fragments arranged by size:
1121 993 685 660 191
SfoI GGC'GCC
Cuts at: 1082 1330 1082
Size: 248 3402
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3650
SmaI CCC'GGG
Cuts at: 140 672 140
Size: 532 3118
SmlI C'TyrA_G
Cuts at: 805 1720 2212 2474 805
Size: 915 492 262 1981
Fragments arranged by size:
1981 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3650
SphI G_CATG'C
Cuts at: 703 1203 703
Size: 500 3150
StuI AGG'CCT
Cuts at: 118 118
Size: 3650
StyI C'CwwG_G
Cuts at: 107 784 1138 107
Size: 677 354 2619
Fragments arranged by size:
2619 677 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 1366 1825 2034 1233
Size: 133 459 209 2849
Fragments arranged by size:
2849 459 209 133
TatI w'GTAC_w
Cuts at: 3165 3165
Size: 3650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 430 462 464 496 551 583 3167 3199 430
Size: 32 2 32 55 32 2584 32 881
Fragments arranged by size:
2584 881 55 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1447 1447
Size: 3650
XbaI T'CTAG_A
Cuts at: 681 681
Size: 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 1145
Size: 3650
XmaI C'CCGG_G
Cuts at: 138 670 138
Size: 532 3118
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI