Restriction map for
pF1KB6866
(Circular) (Six-base) MAPSORT of: pF1KB6866.seq Check: 5521 from: 1 to: 3701
pF1KB6866 3701 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 739 739
Size: 3701
Acc65I G'GTAC_C
Cuts at: 717 717
Size: 3701
AclI AA'CG_TT
Cuts at: 3541 3541
Size: 3701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1958 2705 2973 1526
Size: 432 747 268 2254
Fragments arranged by size:
2254 747 432 268
AflIII A'CryG_T
Cuts at: 1053 2157 3134 1053
Size: 1104 977 1620
Fragments arranged by size:
1620 1104 977
AgeI A'CCGG_T
Cuts at: 2089 2089
Size: 3701
AhdI GACnn_n'nnGTC
Cuts at: 241 241
Size: 3701
AlwNI CAG_nnn'CTG
Cuts at: 1006 2573 1006
Size: 1567 2134
ApaI G_GGCC'C
Cuts at: 598 645 598
Size: 47 3654
ApaLI G'TGCA_C
Cuts at: 2471 2471
Size: 3701
ApoI r'AATT_y
Cuts at: 705 3054 3310 705
Size: 2349 256 1096
Fragments arranged by size:
2349 1096 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3701
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
AvaI C'yCGr_G
Cuts at: 591 598 644 721 591
Size: 7 46 77 3571
Fragments arranged by size:
3571 77 46 7
BamHI G'GATC_C
Cuts at: 438 521 726 438
Size: 83 205 3413
Fragments arranged by size:
3413 205 83
BanI G'GyrC_C
Cuts at: 717 1131 1379 1414 717
Size: 414 248 35 3004
Fragments arranged by size:
3004 414 248 35
BanII G_rGCy'C
Cuts at: 598 611 645 715 1745 598
Size: 13 34 70 1030 2554
Fragments arranged by size:
2554 1030 70 34 13
BbeI G_GCGC'C
Cuts at: 1135 1383 1135
Size: 248 3453
BciVI GTATCCnnnnn_n'
Cuts at: 1599 2360 3701 1599
Size: 761 1341 1599
Fragments arranged by size:
1599 1341 761
BclI T'GATC_A
Cuts at: 1221 1221
Size: 3701
BglII A'GATC_T
Cuts at: 1216 3253 1216
Size: 2037 1664
BlpI GC'TnA_GC
Cuts at: 813 813
Size: 3701
Bme1580I G_kGCm'C
Cuts at: 598 630 645 1326 1419 2475 598
Size: 32 15 681 93 1056 1824
Fragments arranged by size:
1824 1056 681 93 32 15
BmrI ACTGGGnnnn_n'
Cuts at: 214 294 913 994 1329 3473 214
Size: 80 619 81 335 2144 442
Fragments arranged by size:
2144 619 442 335 81 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2068 2068
Size: 3701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 368
Size: 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 877 1756 2248 2546 398
Size: 479 879 492 298 1553
Fragments arranged by size:
1553 879 492 479 298
BsaI GGTCTCn'nnnn_
Cuts at: 3405 3405
Size: 3701
BsaAI yAC'GTr
Cuts at: 1054 1054
Size: 3701
BsaBI GATnn'nnATC
Cuts at: 6 1215 3252 6
Size: 1209 2037 455
Fragments arranged by size:
2037 1209 455
BsaHI Gr'CG_yC
Cuts at: 1132 1380 2082 3578 1132
Size: 248 702 1496 1255
Fragments arranged by size:
1496 1255 702 248
BsaWI w'CCGG_w
Cuts at: 890 1411 2089 2363 2510 2921 890
Size: 521 678 274 147 411 1670
Fragments arranged by size:
1670 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 341 371 466 496 341
Size: 30 95 30 3546
Fragments arranged by size:
3546 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 487 500 487
Size: 13 3688
BseYI C'CCAG_C
Cuts at: 1126 1884 2461 2957 1126
Size: 758 577 496 1870
Fragments arranged by size:
1870 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 272 171
Size: 101 3600
BsiEI CG_ry'CG
Cuts at: 104 1243 1289 2497 104
Size: 1139 46 1208 1308
Fragments arranged by size:
1308 1208 1139 46
BsiHKAI G_wGCw'C
Cuts at: 611 715 1493 1683 2475 611
Size: 104 778 190 792 1837
Fragments arranged by size:
1837 792 778 190 104
Bsp1286I G_dGCh'C
Cuts at: 598 611 630 645 715 1326 1419 1493 1683
Size: 13 19 15 70 611 93 74 190
Cuts at: 1683 1745 2475 598
Size: 62 730 1824
Fragments arranged by size:
1824 730 611 190 93 74 70 62
19 15 13
BspEI T'CCGG_A
Cuts at: 2921 2921
Size: 3701
BspHI T'CATG_A
Cuts at: 2877 2877
Size: 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 751 965 1267 1648 392
Size: 359 214 302 381 2445
Fragments arranged by size:
2445 381 359 302 214
BsrBI CCG'CTC
Cuts at: 50 1993 2047 3531 3696 50
Size: 1943 54 1484 165 55
Fragments arranged by size:
1943 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 1613
Size: 3701
BsrFI r'CCGG_y
Cuts at: 1699 1880 2089 1699
Size: 181 209 3311
Fragments arranged by size:
3311 209 181
BssSI C'ACGA_G
Cuts at: 1972 2330 1972
Size: 358 3343
BstAPI GCAn_nnn'nTGC
Cuts at: 1078 1078
Size: 3701
BstBI TT'CG_AA
Cuts at: 2062 2062
Size: 3701
BstYI r'GATC_y
Cuts at: 1 192 438 521 726 1193 1216 1551 1797
Size: 191 246 83 205 467 23 335 246
Cuts at: 1797 2809 2895 2907 3253 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 246 246 205
191 86 83 23 12
Bsu36I CC'TnA_GG
Cuts at: 512 512
Size: 3701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1651 1832 1995 1651
Size: 181 163 3357
Fragments arranged by size:
3357 181 163
BtsI GCAGTG_nn'
Cuts at: 143 944 143
Size: 801 2900
DraI TTT'AAA
Cuts at: 700 700
Size: 3701
DrdI GACnn_nn'nnGTC
Cuts at: 1407 2265 1407
Size: 858 2843
EaeI y'GGCC_r
Cuts at: 1286 1460 1851 1878 3223 1286
Size: 174 391 27 1345 1764
Fragments arranged by size:
1764 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1286 1286
Size: 3701
EarI CTCTTCn'nnn_
Cuts at: 356 1724 1934 356
Size: 1368 210 2123
Fragments arranged by size:
2123 1368 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1909 2219 2365 3511 1909
Size: 310 146 1146 2099
Fragments arranged by size:
2099 1146 310 146
EcoICRI GAG'CTC
Cuts at: 609 713 609
Size: 104 3597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 1526 1958 2705 2973 368
Size: 1158 432 747 268 1096
Fragments arranged by size:
1158 1096 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 243 366 454 595 641 840 243
Size: 123 88 141 46 199 3104
Fragments arranged by size:
3104 199 141 123 88 46
EcoRI G'AATT_C
Cuts at: 705 705
Size: 3701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1720 1720
Size: 3701
FspI TGC'GCA
Cuts at: 1482 1482
Size: 3701
HaeII r_GCGC'y
Cuts at: 1135 1383 2405 3384 1135
Size: 248 1022 979 1452
Fragments arranged by size:
1452 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 513 545 565 597 1543 1573 1575
Size: 32 254 32 20 32 946 30 2
Cuts at: 1575 1605 227
Size: 30 2323
Fragments arranged by size:
2323 946 254 32 32 32 30 30
20 2
HincII GTy'rAC
Cuts at: 740 740
Size: 3701
KasI G'GCGC_C
Cuts at: 1131 1379 1131
Size: 248 3453
KpnI G_GTAC'C
Cuts at: 721 721
Size: 3701
MluI A'CGCG_T
Cuts at: 3134 3134
Size: 3701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 466 499 2372 2556 466
Size: 33 1873 184 1611
Fragments arranged by size:
1873 1611 184 33
MscI TGG'CCA
Cuts at: 1462 1462
Size: 3701
MslI CAynn'nnrTG
Cuts at: 1690 1817 1690
Size: 127 3574
MspA1I CmG'CkG
Cuts at: 372 501 812 1126 1486 2499 2744 372
Size: 129 311 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 360 314 311 245 129
NaeI GCC'GGC
Cuts at: 1882 1882
Size: 3701
NarI GG'CG_CC
Cuts at: 1132 1380 1132
Size: 248 3453
NgoMIV G'CCGG_C
Cuts at: 1880 1880
Size: 3701
NspI r_CATG'y
Cuts at: 754 1254 2161 754
Size: 500 907 2294
Fragments arranged by size:
2294 907 500
PciI A'CATG_T
Cuts at: 2157 2157
Size: 3701
PflMI CCAn_nnn'nTGG
Cuts at: 448 448
Size: 3701
PfoI T'CCnGG_A
Cuts at: 531 656 531
Size: 125 3576
PmeI GTTT'AAAC
Cuts at: 700 700
Size: 3701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2840 2872 2840
Size: 32 3669
PpuMI rG'GwC_Cy
Cuts at: 243 366 243
Size: 123 3578
PshAI GACnn'nnGTC
Cuts at: 408 408
Size: 3701
PspOMI G'GGCC_C
Cuts at: 594 641 594
Size: 47 3654
PstI C_TGCA'G
Cuts at: 165 471 500 690 748 1433 165
Size: 306 29 190 58 685 2433
Fragments arranged by size:
2433 685 306 190 58 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3701
PvuII CAG'CTG
Cuts at: 501 1126 1486 501
Size: 625 360 2716
Fragments arranged by size:
2716 625 360
RsrII CG'GwC_CG
Cuts at: 1896 1896
Size: 3701
SacI G_AGCT'C
Cuts at: 611 715 611
Size: 104 3597
SalI G'TCGA_C
Cuts at: 738 738
Size: 3701
SapI GCTCTTCn'nnn_
Cuts at: 1724 1934 1724
Size: 210 3491
SbfI CC_TGCA'GG
Cuts at: 748 748
Size: 3701
ScaI AGT'ACT
Cuts at: 550 3218 550
Size: 2668 1033
SfcI C'TryA_G
Cuts at: 33 161 394 467 496 686 744 1429 2422
Size: 128 233 73 29 190 58 685 993
Cuts at: 2422 2613 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 233 191 190 128 73
58 29
SfoI GGC'GCC
Cuts at: 1133 1381 1133
Size: 248 3453
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3701
SmaI CCC'GGG
Cuts at: 646 723 646
Size: 77 3624
SmlI C'TyrA_G
Cuts at: 413 856 1771 2263 2525 413
Size: 443 915 492 262 1589
Fragments arranged by size:
1589 915 492 443 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3701
SphI G_CATG'C
Cuts at: 754 1254 754
Size: 500 3201
SrfI GCCC'GGGC
Cuts at: 646 646
Size: 3701
StuI AGG'CCT
Cuts at: 229 589 667 229
Size: 360 78 3263
Fragments arranged by size:
3263 360 78
StyI C'CwwG_G
Cuts at: 835 1189 835
Size: 354 3347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 1417 1876 2085 1284
Size: 133 459 209 2900
Fragments arranged by size:
2900 459 209 133
TatI w'GTAC_w
Cuts at: 548 3216 548
Size: 2668 1033
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 194 226 341 373 466 498 3218 3250 194
Size: 32 115 32 93 32 2720 32 645
Fragments arranged by size:
2720 645 115 93 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1498 1498
Size: 3701
XbaI T'CTAG_A
Cuts at: 732 732
Size: 3701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1196 1196
Size: 3701
XmaI C'CCGG_G
Cuts at: 644 721 644
Size: 77 3624
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI