Restriction map for
pF1KB6872
(Circular) (Six-base) MAPSORT of: pF1KB6872.seq Check: 4433 from: 1 to: 3707
pF1KB6872 3707 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 745 745
Size: 3707
Acc65I G'GTAC_C
Cuts at: 723 723
Size: 3707
AclI AA'CG_TT
Cuts at: 3547 3547
Size: 3707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 622 1532 1964 2711 2979 126
Size: 496 910 432 747 268 854
Fragments arranged by size:
910 854 747 496 432 268
AflIII A'CryG_T
Cuts at: 264 1059 2163 3140 264
Size: 795 1104 977 831
Fragments arranged by size:
1104 977 831 795
AgeI A'CCGG_T
Cuts at: 2095 2095
Size: 3707
AlwNI CAG_nnn'CTG
Cuts at: 1012 2579 1012
Size: 1567 2140
ApaLI G'TGCA_C
Cuts at: 2477 2477
Size: 3707
ApoI r'AATT_y
Cuts at: 711 3060 3316 711
Size: 2349 256 1102
Fragments arranged by size:
2349 1102 256
AseI AT'TA_AT
Cuts at: 20 386 20
Size: 366 3341
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
AvaI C'yCGr_G
Cuts at: 727 727
Size: 3707
AvrII C'CTAG_G
Cuts at: 162 162
Size: 3707
BamHI G'GATC_C
Cuts at: 732 732
Size: 3707
BanI G'GyrC_C
Cuts at: 723 1137 1385 1420 723
Size: 414 248 35 3010
Fragments arranged by size:
3010 414 248 35
BanII G_rGCy'C
Cuts at: 721 1751 721
Size: 1030 2677
BbeI G_GCGC'C
Cuts at: 1141 1389 1141
Size: 248 3459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 337 371 337
Size: 34 3673
BciVI GTATCCnnnnn_n'
Cuts at: 1605 2366 3707 1605
Size: 761 1341 1605
Fragments arranged by size:
1605 1341 761
BclI T'GATC_A
Cuts at: 1227 1227
Size: 3707
BglII A'GATC_T
Cuts at: 1222 3259 1222
Size: 2037 1670
BlpI GC'TnA_GC
Cuts at: 819 819
Size: 3707
Bme1580I G_kGCm'C
Cuts at: 344 1332 1425 2481 344
Size: 988 93 1056 1570
Fragments arranged by size:
1570 1056 988 93
BmgBI CAC'GTC
Cuts at: 355 355
Size: 3707
BmrI ACTGGGnnnn_n'
Cuts at: 684 919 1000 1335 3479 684
Size: 235 81 335 2144 912
Fragments arranged by size:
2144 912 335 235 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 3707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 883 1762 2254 2552 634
Size: 249 879 492 298 1789
Fragments arranged by size:
1789 879 492 298 249
BsaI GGTCTCn'nnnn_
Cuts at: 3411 3411
Size: 3707
BsaAI yAC'GTr
Cuts at: 1060 1060
Size: 3707
BsaBI GATnn'nnATC
Cuts at: 6 1221 3258 6
Size: 1215 2037 455
Fragments arranged by size:
2037 1215 455
BsaHI Gr'CG_yC
Cuts at: 1138 1386 2088 3584 1138
Size: 248 702 1496 1261
Fragments arranged by size:
1496 1261 702 248
BsaWI w'CCGG_w
Cuts at: 896 1417 2095 2369 2516 2927 896
Size: 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 378 1132 1890 2467 2963 378
Size: 754 758 577 496 1122
Fragments arranged by size:
1122 758 754 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 660 434
Size: 226 3481
BsiEI CG_ry'CG
Cuts at: 104 1249 1295 2503 104
Size: 1145 46 1208 1308
Fragments arranged by size:
1308 1208 1145 46
BsiHKAI G_wGCw'C
Cuts at: 155 650 721 1499 1689 2481 155
Size: 495 71 778 190 792 1381
Fragments arranged by size:
1381 792 778 495 190 71
BsmI GAATG_Cn'
Cuts at: 618 618
Size: 3707
Bsp1286I G_dGCh'C
Cuts at: 155 344 650 721 1332 1425 1499 1689 1751
Size: 189 306 71 611 93 74 190 62
Cuts at: 1751 2481 155
Size: 730 1381
Fragments arranged by size:
1381 730 611 306 190 189 93 74
71 62
BspEI T'CCGG_A
Cuts at: 2927 2927
Size: 3707
BspHI T'CATG_A
Cuts at: 2883 2883
Size: 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 757 971 1273 1654 757
Size: 214 302 381 2810
Fragments arranged by size:
2810 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1999 2053 3537 3702 50
Size: 1949 54 1484 165 55
Fragments arranged by size:
1949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1619 1619
Size: 3707
BsrFI r'CCGG_y
Cuts at: 1705 1886 2095 1705
Size: 181 209 3317
Fragments arranged by size:
3317 209 181
BsrGI T'GTAC_A
Cuts at: 170 170
Size: 3707
BssSI C'ACGA_G
Cuts at: 343 430 1978 2336 343
Size: 87 1548 358 1714
Fragments arranged by size:
1714 1548 358 87
BstAPI GCAn_nnn'nTGC
Cuts at: 1084 1084
Size: 3707
BstBI TT'CG_AA
Cuts at: 2068 2068
Size: 3707
BstYI r'GATC_y
Cuts at: 1 128 732 1199 1222 1557 1803 2815 2901
Size: 127 604 467 23 335 246 1012 86
Cuts at: 2901 2913 3259 1
Size: 12 346 449
Fragments arranged by size:
1012 604 467 449 346 335 246 127
86 23 12
BtgI C'CryG_G
Cuts at: 623 623
Size: 3707
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1657 1838 2001 1657
Size: 181 163 3363
Fragments arranged by size:
3363 181 163
BtsI GCAGTG_nn'
Cuts at: 153 648 950 153
Size: 495 302 2910
Fragments arranged by size:
2910 495 302
DraI TTT'AAA
Cuts at: 706 706
Size: 3707
DrdI GACnn_nn'nnGTC
Cuts at: 1413 2271 1413
Size: 858 2849
EaeI y'GGCC_r
Cuts at: 1292 1466 1857 1884 3229 1292
Size: 174 391 27 1345 1770
Fragments arranged by size:
1770 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1292 1292
Size: 3707
EarI CTCTTCn'nnn_
Cuts at: 233 287 1730 1940 233
Size: 54 1443 210 2000
Fragments arranged by size:
2000 1443 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1915 2225 2371 3517 1915
Size: 310 146 1146 2105
Fragments arranged by size:
2105 1146 310 146
EcoICRI GAG'CTC
Cuts at: 719 719
Size: 3707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 126 139 622 1532 1964 2711 2979 126
Size: 13 483 910 432 747 268 854
Fragments arranged by size:
910 854 747 483 432 268 13
EcoO109I rG'GnC_Cy
Cuts at: 846 846
Size: 3707
EcoRI G'AATT_C
Cuts at: 711 711
Size: 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 466 1726 466
Size: 1260 2447
FspI TGC'GCA
Cuts at: 1488 1488
Size: 3707
HaeII r_GCGC'y
Cuts at: 1141 1389 2411 3390 1141
Size: 248 1022 979 1458
Fragments arranged by size:
1458 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1549 1579 1581 1611 1549
Size: 30 2 30 3645
Fragments arranged by size:
3645 30 30 2
HincII GTy'rAC
Cuts at: 746 746
Size: 3707
KasI G'GCGC_C
Cuts at: 1137 1385 1137
Size: 248 3459
KpnI G_GTAC'C
Cuts at: 727 727
Size: 3707
MfeI C'AATT_G
Cuts at: 302 302
Size: 3707
MluI A'CGCG_T
Cuts at: 3140 3140
Size: 3707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 295 307 2378 2562 295
Size: 12 2071 184 1440
Fragments arranged by size:
2071 1440 184 12
MscI TGG'CCA
Cuts at: 1468 1468
Size: 3707
MslI CAynn'nnrTG
Cuts at: 628 1696 1823 628
Size: 1068 127 2512
Fragments arranged by size:
2512 1068 127
MspA1I CmG'CkG
Cuts at: 680 818 1132 1492 2505 2750 680
Size: 138 314 360 1013 245 1637
Fragments arranged by size:
1637 1013 360 314 245 138
NaeI GCC'GGC
Cuts at: 1888 1888
Size: 3707
NarI GG'CG_CC
Cuts at: 1138 1386 1138
Size: 248 3459
NcoI C'CATG_G
Cuts at: 623 623
Size: 3707
NgoMIV G'CCGG_C
Cuts at: 1886 1886
Size: 3707
NspI r_CATG'y
Cuts at: 240 268 760 1260 2167 240
Size: 28 492 500 907 1780
Fragments arranged by size:
1780 907 500 492 28
PciI A'CATG_T
Cuts at: 264 2163 264
Size: 1899 1808
PmeI GTTT'AAAC
Cuts at: 706 706
Size: 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3675
PstI C_TGCA'G
Cuts at: 754 1439 754
Size: 685 3022
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3707
PvuII CAG'CTG
Cuts at: 1132 1492 1132
Size: 360 3347
RsrII CG'GwC_CG
Cuts at: 1902 1902
Size: 3707
SacI G_AGCT'C
Cuts at: 721 721
Size: 3707
SalI G'TCGA_C
Cuts at: 744 744
Size: 3707
SapI GCTCTTCn'nnn_
Cuts at: 287 1730 1940 287
Size: 1443 210 2054
Fragments arranged by size:
2054 1443 210
SbfI CC_TGCA'GG
Cuts at: 754 754
Size: 3707
ScaI AGT'ACT
Cuts at: 3224 3224
Size: 3707
SfcI C'TryA_G
Cuts at: 33 750 1435 2428 2619 33
Size: 717 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 191
SfoI GGC'GCC
Cuts at: 1139 1387 1139
Size: 248 3459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3707
SmaI CCC'GGG
Cuts at: 729 729
Size: 3707
SmlI C'TyrA_G
Cuts at: 649 862 1777 2269 2531 649
Size: 213 915 492 262 1825
Fragments arranged by size:
1825 915 492 262 213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3707
SphI G_CATG'C
Cuts at: 760 1260 760
Size: 500 3207
StyI C'CwwG_G
Cuts at: 162 623 841 1195 162
Size: 461 218 354 2674
Fragments arranged by size:
2674 461 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1423 1882 2091 1290
Size: 133 459 209 2906
Fragments arranged by size:
2906 459 209 133
TatI w'GTAC_w
Cuts at: 170 3222 170
Size: 3052 655
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 3675
Tth111I GACn'n_nGTC
Cuts at: 1504 1504
Size: 3707
XbaI T'CTAG_A
Cuts at: 738 738
Size: 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1202 1202
Size: 3707
XmaI C'CCGG_G
Cuts at: 727 727
Size: 3707
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI