Restriction map for
pF1KB6879
(Circular) (Six-base) MAPSORT of: pF1KB6879.seq Check: 2752 from: 1 to: 3653
pF1KB6879 3653 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 691 691
Size: 3653
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1478 1910 2657 2925 1478
Size: 432 747 268 2206
Fragments arranged by size:
2206 747 432 268
AflIII A'CryG_T
Cuts at: 1005 2109 3086 1005
Size: 1104 977 1572
Fragments arranged by size:
1572 1104 977
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 270 958 2525 270
Size: 688 1567 1398
Fragments arranged by size:
1567 1398 688
ApaI G_GGCC'C
Cuts at: 139 139
Size: 3653
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 209 673 209
Size: 464 3189
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 539 572 539
Size: 33 3620
BamHI G'GATC_C
Cuts at: 372 678 372
Size: 306 3347
BanI G'GyrC_C
Cuts at: 669 1083 1331 1366 669
Size: 414 248 35 2956
Fragments arranged by size:
2956 414 248 35
BanII G_rGCy'C
Cuts at: 139 667 1697 139
Size: 528 1030 2095
Fragments arranged by size:
2095 1030 528
BbeI G_GCGC'C
Cuts at: 1087 1335 1087
Size: 248 3405
BbsI GAAGACnn'nnnn_
Cuts at: 250 510 250
Size: 260 3393
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglI GCCn_nnn'nGGC
Cuts at: 288 404 288
Size: 116 3537
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 139 1278 1371 2427 139
Size: 1139 93 1056 1365
Fragments arranged by size:
1365 1139 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 865 946 1281 3425 865
Size: 81 335 2144 1093
Fragments arranged by size:
2144 1093 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 434 2020 434
Size: 1586 2067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 567 292
Size: 275 3378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 405 3357 405
Size: 2952 701
BsaAI yAC'GTr
Cuts at: 1006 1006
Size: 3653
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 1084 1332 2034 3530 1084
Size: 248 702 1496 1207
Fragments arranged by size:
1496 1207 702 248
BsaWI w'CCGG_w
Cuts at: 420 842 1363 2041 2315 2462 2873 420
Size: 422 521 678 274 147 411 1200
Fragments arranged by size:
1200 678 521 422 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 565 595 565
Size: 30 3623
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 139 136
Size: 3 3650
BseYI C'CCAG_C
Cuts at: 313 576 1078 1836 2413 2909 313
Size: 263 502 758 577 496 1057
Fragments arranged by size:
1057 758 577 502 496 263
BsiEI CG_ry'CG
Cuts at: 104 1195 1241 2449 104
Size: 1091 46 1208 1308
Fragments arranged by size:
1308 1208 1091 46
BsiHKAI G_wGCw'C
Cuts at: 667 1445 1635 2427 667
Size: 778 190 792 1893
Fragments arranged by size:
1893 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 139 667 1278 1371 1445 1635 1697 2427 139
Size: 528 611 93 74 190 62 730 1365
Fragments arranged by size:
1365 730 611 528 190 93 74 62
BspEI T'CCGG_A
Cuts at: 420 2873 420
Size: 2453 1200
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 159 1945 1999 3483 3648 50
Size: 109 1786 54 1484 165 55
Fragments arranged by size:
1786 1484 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 1565 1565
Size: 3653
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 1924 2282 1924
Size: 358 3295
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 182 2014 182
Size: 1832 1821
BstEII G'GTnAC_C
Cuts at: 571 571
Size: 3653
BstXI CCAn_nnnn'nTGG
Cuts at: 448 448
Size: 3653
BstYI r'GATC_y
Cuts at: 1 372 558 678 1145 1168 1503 1749 2761
Size: 371 186 120 467 23 335 246 1012
Cuts at: 2761 2847 2859 3205 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 371 346 335 246 186
120 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3653
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 234 250 280 1238 1412 1803 1830 3175 234
Size: 16 30 958 174 391 27 1345 712
Fragments arranged by size:
1345 958 712 391 174 30 27 16
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 607 1676 1886 607
Size: 1069 210 2374
Fragments arranged by size:
2374 1069 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 1861 2171 2317 3463 529
Size: 1332 310 146 1146 719
Fragments arranged by size:
1332 1146 719 310 146
EcoICRI GAG'CTC
Cuts at: 665 665
Size: 3653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 567 1478 1910 2657 2925 292
Size: 275 911 432 747 268 1020
Fragments arranged by size:
1020 911 747 432 275 268
EcoO109I rG'GnC_Cy
Cuts at: 135 136 792 135
Size: 1 656 2996
Fragments arranged by size:
2996 656 1
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 1434 1434
Size: 3653
HaeII r_GCGC'y
Cuts at: 1087 1335 2357 3336 1087
Size: 248 1022 979 1404
Fragments arranged by size:
1404 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 692 692
Size: 3653
KasI G'GCGC_C
Cuts at: 1083 1331 1083
Size: 248 3405
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2508 2324
Size: 184 3469
MscI TGG'CCA
Cuts at: 236 252 1414 236
Size: 16 1162 2475
Fragments arranged by size:
2475 1162 16
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 118 587 764 1078 1438 2451 2696 118
Size: 469 177 314 360 1013 245 1075
Fragments arranged by size:
1075 1013 469 360 314 245 177
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 1084 1332 1084
Size: 248 3405
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NruI TCG'CGA
Cuts at: 155 155
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PasI CC'CwG_GG
Cuts at: 402 402
Size: 3653
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PspOMI G'GGCC_C
Cuts at: 135 135
Size: 3653
PstI C_TGCA'G
Cuts at: 700 1385 700
Size: 685 2968
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 587 1078 1438 587
Size: 491 360 2802
Fragments arranged by size:
2802 491 360
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 667 667
Size: 3653
SalI G'TCGA_C
Cuts at: 690 690
Size: 3653
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 696 1381 2374 2565 33
Size: 663 685 993 191 1121
Fragments arranged by size:
1121 993 685 663 191
SfoI GGC'GCC
Cuts at: 1085 1333 1085
Size: 248 3405
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 808 1723 2215 2477 808
Size: 915 492 262 1984
Fragments arranged by size:
1984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 107 787 1141 107
Size: 680 354 2619
Fragments arranged by size:
2619 680 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 590 1236 1369 1828 2037 590
Size: 646 133 459 209 2206
Fragments arranged by size:
2206 646 459 209 133
TatI w'GTAC_w
Cuts at: 242 3168 242
Size: 2926 727
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 3170 3202 565
Size: 32 2573 32 1016
Fragments arranged by size:
2573 1016 32 32
Tth111I GACn'n_nGTC
Cuts at: 569 1450 569
Size: 881 2772
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 475 584 1148 475
Size: 109 564 2980
Fragments arranged by size:
2980 564 109
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI