Restriction map for
pF1KB6880
(Circular) (Six-base) MAPSORT of: pF1KB6880.seq Check: 1275 from: 1 to: 3716
pF1KB6880 3716 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AflIII A'CryG_T
Cuts at: 132 1068 2172 3149 132
Size: 936 1104 977 699
Fragments arranged by size:
1104 977 936 699
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 553 585 553
Size: 32 3684
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 437 720 3069 3325 437
Size: 283 2349 256 828
Fragments arranged by size:
2349 828 283 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 345 736 345
Size: 391 3325
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 732 1146 1394 1429 732
Size: 414 248 35 3019
Fragments arranged by size:
3019 414 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbvCI CC'TCA_GC
Cuts at: 667 667
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 375 1231 3268 375
Size: 856 2037 823
Fragments arranged by size:
2037 856 823
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 345 1341 1434 2490 345
Size: 996 93 1056 1571
Fragments arranged by size:
1571 1056 996 93
BmrI ACTGGGnnnn_n'
Cuts at: 512 928 1009 1344 3488 512
Size: 416 81 335 2144 740
Fragments arranged by size:
2144 740 416 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 563 392
Size: 171 3545
Bpu10I CC'TnA_GC
Cuts at: 667 667
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 620 1069 620
Size: 449 3267
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 553
Size: 30 3686
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 574 601 154
Size: 420 27 3269
Fragments arranged by size:
3269 420 27
BseYI C'CCAG_C
Cuts at: 264 671 1141 1899 2476 2972 264
Size: 407 470 758 577 496 1008
Fragments arranged by size:
1008 758 577 496 470 407
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 345 730 1341 1434 1508 1698 1760 2490 345
Size: 385 611 93 74 190 62 730 1571
Fragments arranged by size:
1571 730 611 385 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 1714 1895 2104 1714
Size: 181 209 3326
Fragments arranged by size:
3326 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 288 375 741 1208 1231 1566 1812 2824
Size: 287 87 366 467 23 335 246 1012
Cuts at: 2824 2910 2922 3268 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 366 346 335 287 246
87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 557 557
Size: 3716
BtgI C'CryG_G
Cuts at: 359 359
Size: 3716
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 1422 2280 1422
Size: 858 2858
EaeI y'GGCC_r
Cuts at: 1301 1475 1866 1893 3238 1301
Size: 174 391 27 1345 1779
Fragments arranged by size:
1779 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 563 1541 1973 2720 2988 392
Size: 171 978 432 747 268 1120
Fragments arranged by size:
1120 978 747 432 268 171
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 3716
EcoO109I rG'GnC_Cy
Cuts at: 855 855
Size: 3716
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 654 1735 654
Size: 1081 2635
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 1150 1398 2420 3399 1150
Size: 248 1022 979 1467
Fragments arranged by size:
1467 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 1558 1588 1590 1620 451
Size: 32 1075 30 2 30 2547
Fragments arranged by size:
2547 1075 32 30 30 2
HincII GTy'rAC
Cuts at: 755 755
Size: 3716
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 1477 1477
Size: 3716
MslI CAynn'nnrTG
Cuts at: 1705 1832 1705
Size: 127 3589
MspA1I CmG'CkG
Cuts at: 671 827 1141 1501 2514 2759 671
Size: 156 314 360 1013 245 1628
Fragments arranged by size:
1628 1013 360 314 245 156
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 359 359
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NspI r_CATG'y
Cuts at: 136 606 769 1269 2176 136
Size: 470 163 500 907 1676
Fragments arranged by size:
1676 907 500 470 163
PciI A'CATG_T
Cuts at: 132 2172 132
Size: 2040 1676
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 553 585 2855 2887 553
Size: 32 2270 32 1382
Fragments arranged by size:
2270 1382 32 32
PsiI TTA'TAA
Cuts at: 179 179
Size: 3716
PstI C_TGCA'G
Cuts at: 215 763 1448 215
Size: 548 685 2483
Fragments arranged by size:
2483 685 548
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 671 1141 1501 671
Size: 470 360 2886
Fragments arranged by size:
2886 470 360
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 211 495 759 1444 2437 2628 33
Size: 178 284 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 284 264 191 178
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 347 738 347
Size: 391 3325
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 606 769 1269 606
Size: 163 500 3053
Fragments arranged by size:
3053 500 163
StuI AGG'CCT
Cuts at: 473 473
Size: 3716
StyI C'CwwG_G
Cuts at: 359 596 850 1204 359
Size: 237 254 354 2871
Fragments arranged by size:
2871 354 254 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 248 3231 248
Size: 2983 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 616 648 3233 3265 616
Size: 32 2585 32 1067
Fragments arranged by size:
2585 1067 32 32
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 603 1211 603
Size: 608 3108
XmaI C'CCGG_G
Cuts at: 345 736 345
Size: 391 3325
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI