Restriction map for
pF1KB6881
(Circular) (Six-base) MAPSORT of: pF1KB6881.seq Check: 747 from: 1 to: 3656
pF1KB6881 3656 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 383 694 383
Size: 311 3345
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2660 2928 1481
Size: 432 747 268 2209
Fragments arranged by size:
2209 747 432 268
AflIII A'CryG_T
Cuts at: 1008 2112 3089 1008
Size: 1104 977 1575
Fragments arranged by size:
1575 1104 977
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 495 961 2528 495
Size: 466 1567 1623
Fragments arranged by size:
1623 1567 466
ApaLI G'TGCA_C
Cuts at: 2426 2426
Size: 3656
ApoI r'AATT_y
Cuts at: 660 3009 3265 660
Size: 2349 256 1051
Fragments arranged by size:
2349 1051 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 416 676 416
Size: 260 3396
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 672 1086 1334 1369 672
Size: 414 248 35 2959
Fragments arranged by size:
2959 414 248 35
BanII G_rGCy'C
Cuts at: 455 670 1700 455
Size: 215 1030 2411
Fragments arranged by size:
2411 1030 215
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 566 532
Size: 34 3622
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglII A'GATC_T
Cuts at: 1171 3208 1171
Size: 2037 1619
BlpI GC'TnA_GC
Cuts at: 768 768
Size: 3656
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2430 1281
Size: 93 1056 2507
Fragments arranged by size:
2507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 232 868 949 1284 3428 232
Size: 636 81 335 2144 460
Fragments arranged by size:
2144 636 460 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 360
Size: 3656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 832 1711 2203 2501 118
Size: 714 879 492 298 1273
Fragments arranged by size:
1273 879 714 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 3656
BsaAI yAC'GTr
Cuts at: 1009 1009
Size: 3656
BsaBI GATnn'nnATC
Cuts at: 6 163 1170 3207 6
Size: 157 1007 2037 455
Fragments arranged by size:
2037 1007 455 157
BsaHI Gr'CG_yC
Cuts at: 1087 1335 2037 3533 1087
Size: 248 702 1496 1210
Fragments arranged by size:
1496 1210 702 248
BsaWI w'CCGG_w
Cuts at: 845 1366 2044 2318 2465 2876 845
Size: 521 678 274 147 411 1625
Fragments arranged by size:
1625 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 534 1081 1839 2416 2912 534
Size: 547 758 577 496 1278
Fragments arranged by size:
1278 758 577 547 496
BsiEI CG_ry'CG
Cuts at: 104 1198 1244 2452 104
Size: 1094 46 1208 1308
Fragments arranged by size:
1308 1208 1094 46
BsiHKAI G_wGCw'C
Cuts at: 455 670 1448 1638 2430 455
Size: 215 778 190 792 1681
Fragments arranged by size:
1681 792 778 215 190
BsmI GAATG_Cn'
Cuts at: 440 543 440
Size: 103 3553
Bsp1286I G_dGCh'C
Cuts at: 455 670 1281 1374 1448 1638 1700 2430 455
Size: 215 611 93 74 190 62 730 1681
Fragments arranged by size:
1681 730 611 215 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 2832 2832
Size: 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 920 1222 1603 706
Size: 214 302 381 2759
Fragments arranged by size:
2759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 1568 396
Size: 1172 2484
BsrFI r'CCGG_y
Cuts at: 1654 1835 2044 1654
Size: 181 209 3266
Fragments arranged by size:
3266 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 492 681 1148 1171 1506 1752 2764 2850
Size: 491 189 467 23 335 246 1012 86
Cuts at: 2850 2862 3208 1
Size: 12 346 449
Fragments arranged by size:
1012 491 467 449 346 335 246 189
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1606 1787 1950 1606
Size: 181 163 3312
Fragments arranged by size:
3312 181 163
BtsI GCAGTG_nn'
Cuts at: 899 899
Size: 3656
DraI TTT'AAA
Cuts at: 655 655
Size: 3656
DrdI GACnn_nn'nnGTC
Cuts at: 1362 2220 1362
Size: 858 2798
EaeI y'GGCC_r
Cuts at: 315 626 1241 1415 1806 1833 3178 315
Size: 311 615 174 391 27 1345 793
Fragments arranged by size:
1345 793 615 391 311 174 27
EagI C'GGCC_G
Cuts at: 1241 1241
Size: 3656
EarI CTCTTCn'nnn_
Cuts at: 416 1679 1889 416
Size: 1263 210 2183
Fragments arranged by size:
2183 1263 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 1864 2174 2320 3466 457
Size: 1407 310 146 1146 647
Fragments arranged by size:
1407 1146 647 310 146
EcoICRI GAG'CTC
Cuts at: 453 668 453
Size: 215 3441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 1481 1913 2660 2928 360
Size: 1121 432 747 268 1088
Fragments arranged by size:
1121 1088 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 795 795
Size: 3656
EcoRI G'AATT_C
Cuts at: 660 660
Size: 3656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 1675
Size: 3656
FspI TGC'GCA
Cuts at: 163 1437 163
Size: 1274 2382
FspAI rTGC'GCAy
Cuts at: 163 163
Size: 3656
HaeII r_GCGC'y
Cuts at: 1090 1338 2360 3339 1090
Size: 248 1022 979 1407
Fragments arranged by size:
1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1498 1528 1530 1560 1498
Size: 30 2 30 3594
Fragments arranged by size:
3594 30 30 2
HincII GTy'rAC
Cuts at: 384 695 384
Size: 311 3345
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2511 2327
Size: 184 3472
MscI TGG'CCA
Cuts at: 317 628 1417 317
Size: 311 789 2556
Fragments arranged by size:
2556 789 311
MslI CAynn'nnrTG
Cuts at: 169 1645 1772 169
Size: 1476 127 2053
Fragments arranged by size:
2053 1476 127
MspA1I CmG'CkG
Cuts at: 593 767 1081 1441 2454 2699 593
Size: 174 314 360 1013 245 1550
Fragments arranged by size:
1550 1013 360 314 245 174
NaeI GCC'GGC
Cuts at: 1837 1837
Size: 3656
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NcoI C'CATG_G
Cuts at: 107 107
Size: 3656
NgoMIV G'CCGG_C
Cuts at: 1835 1835
Size: 3656
NspI r_CATG'y
Cuts at: 709 1209 2116 709
Size: 500 907 2249
Fragments arranged by size:
2249 907 500
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 3656
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PstI C_TGCA'G
Cuts at: 703 1388 703
Size: 685 2971
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 1081 1441 1081
Size: 360 3296
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 455 670 455
Size: 215 3441
SalI G'TCGA_C
Cuts at: 382 693 382
Size: 311 3345
SapI GCTCTTCn'nnn_
Cuts at: 416 1679 1889 416
Size: 1263 210 2183
Fragments arranged by size:
2183 1263 210
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 3173 3173
Size: 3656
SfcI C'TryA_G
Cuts at: 33 699 1384 2377 2568 33
Size: 666 685 993 191 1121
Fragments arranged by size:
1121 993 685 666 191
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 418 678 418
Size: 260 3396
SmlI C'TyrA_G
Cuts at: 133 811 1726 2218 2480 133
Size: 678 915 492 262 1309
Fragments arranged by size:
1309 915 678 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StuI AGG'CCT
Cuts at: 614 614
Size: 3656
StyI C'CwwG_G
Cuts at: 107 363 790 1144 107
Size: 256 427 354 2619
Fragments arranged by size:
2619 427 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 301 376 1239 1372 1831 2040 301
Size: 75 863 133 459 209 1917
Fragments arranged by size:
1917 863 459 209 133 75
TatI w'GTAC_w
Cuts at: 465 3171 465
Size: 2706 950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 3624
Tth111I GACn'n_nGTC
Cuts at: 1453 1453
Size: 3656
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1151 1151
Size: 3656
XmaI C'CCGG_G
Cuts at: 416 676 416
Size: 260 3396
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI