Restriction map for
pF1KB6882
(Circular) (Six-base) MAPSORT of: pF1KB6882.seq Check: 2812 from: 1 to: 3716
pF1KB6882 3716 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 482 754 482
Size: 272 3444
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1541 1973 2720 2988 1541
Size: 432 747 268 2269
Fragments arranged by size:
2269 747 432 268
AflIII A'CryG_T
Cuts at: 365 497 1068 2172 3149 365
Size: 132 571 1104 977 932
Fragments arranged by size:
1104 977 932 571 132
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AlwNI CAG_nnn'CTG
Cuts at: 1021 2588 1021
Size: 1567 2149
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 720 3069 3325 720
Size: 2349 256 1111
Fragments arranged by size:
2349 1111 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 256 575 736 256
Size: 319 161 3236
Fragments arranged by size:
3236 319 161
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 385 541 732 1146 1394 1429 385
Size: 156 191 414 248 35 2672
Fragments arranged by size:
2672 414 248 191 156 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 391 425 484 518 391
Size: 34 59 34 3589
Fragments arranged by size:
3589 59 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 388 1341 1434 2490 388
Size: 953 93 1056 1614
Fragments arranged by size:
1614 1056 953 93
BmgBI CAC'GTC
Cuts at: 366 366
Size: 3716
BmrI ACTGGGnnnn_n'
Cuts at: 369 441 928 1009 1344 3488 369
Size: 72 487 81 335 2144 597
Fragments arranged by size:
2144 597 487 335 81 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 436
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 301 3420 301
Size: 3119 597
BsaAI yAC'GTr
Cuts at: 500 1069 500
Size: 569 3147
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 544 574 622 652 544
Size: 30 48 30 3608
Fragments arranged by size:
3608 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 646
Size: 3716
BseYI C'CCAG_C
Cuts at: 144 1141 1899 2476 2972 144
Size: 997 758 577 496 888
Fragments arranged by size:
997 888 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 529
Size: 3716
BsiEI CG_ry'CG
Cuts at: 104 156 1258 1304 2512 104
Size: 52 1102 46 1208 1308
Fragments arranged by size:
1308 1208 1102 52 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 388 730 1341 1434 1508 1698 1760 2490 388
Size: 342 611 93 74 190 62 730 1614
Fragments arranged by size:
1614 730 611 342 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 980 1282 1663 766
Size: 214 302 381 2819
Fragments arranged by size:
2819 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2008 2062 3546 3711 50
Size: 1958 54 1484 165 55
Fragments arranged by size:
1958 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 310 1714 1895 2104 310
Size: 1404 181 209 1922
Fragments arranged by size:
1922 1404 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 741 1208 1231 1566 1812 2824 2910 2922
Size: 740 467 23 335 246 1012 86 12
Cuts at: 2922 3268 1
Size: 346 449
Fragments arranged by size:
1012 740 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 239 299 239
Size: 60 3656
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 698 1666 1847 2010 698
Size: 968 181 163 2404
Fragments arranged by size:
2404 968 181 163
BtsI GCAGTG_nn'
Cuts at: 959 959
Size: 3716
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 283 1422 2280 283
Size: 1139 858 1719
Fragments arranged by size:
1719 1139 858
EaeI y'GGCC_r
Cuts at: 153 242 312 644 1301 1475 1866 1893 3238
Size: 89 70 332 657 174 391 27 1345
Cuts at: 3238 153
Size: 631
Fragments arranged by size:
1345 657 631 391 332 174 89 70
27
EagI C'GGCC_G
Cuts at: 153 1301 153
Size: 1148 2568
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1541 1973 2720 2988 436
Size: 1105 432 747 268 1164
Fragments arranged by size:
1164 1105 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 227 252 261 405 579 855 227
Size: 25 9 144 174 276 3088
Fragments arranged by size:
3088 276 174 144 25 9
EcoRI G'AATT_C
Cuts at: 720 720
Size: 3716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 219 234 1735 219
Size: 15 1501 2200
Fragments arranged by size:
2200 1501 15
FspI TGC'GCA
Cuts at: 372 1497 372
Size: 1125 2591
HaeII r_GCGC'y
Cuts at: 127 145 326 1150 1398 2420 3399 127
Size: 18 181 824 248 1022 979 444
Fragments arranged by size:
1022 979 824 444 248 181 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 676 708 1558 1588 1590 1620 544
Size: 32 100 32 850 30 2 30 2640
Fragments arranged by size:
2640 850 100 32 32 30 30 2
HincII GTy'rAC
Cuts at: 483 755 483
Size: 272 3444
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 244 646 1477 244
Size: 402 831 2483
Fragments arranged by size:
2483 831 402
MslI CAynn'nnrTG
Cuts at: 1705 1832 1705
Size: 127 3589
MspA1I CmG'CkG
Cuts at: 152 624 827 1141 1501 2514 2759 152
Size: 472 203 314 360 1013 245 1109
Fragments arranged by size:
1109 1013 472 360 314 245 203
NaeI GCC'GGC
Cuts at: 1897 1897
Size: 3716
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 239 239
Size: 3716
NgoMIV G'CCGG_C
Cuts at: 1895 1895
Size: 3716
NotI GC'GGCC_GC
Cuts at: 153 153
Size: 3716
NspI r_CATG'y
Cuts at: 769 1269 2176 769
Size: 500 907 2309
Fragments arranged by size:
2309 907 500
PciI A'CATG_T
Cuts at: 2172 2172
Size: 3716
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 2855
Size: 32 3684
PpuMI rG'GwC_Cy
Cuts at: 227 252 405 579 227
Size: 25 153 174 3364
Fragments arranged by size:
3364 174 153 25
PstI C_TGCA'G
Cuts at: 275 763 1448 275
Size: 488 685 2543
Fragments arranged by size:
2543 685 488
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 1141 1501 1141
Size: 360 3356
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 481 753 481
Size: 272 3444
SanDI GG'GwC_CC
Cuts at: 579 579
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 430 514 3233 430
Size: 84 2719 913
Fragments arranged by size:
2719 913 84
SexAI A'CCwGG_T
Cuts at: 229 229
Size: 3716
SfcI C'TryA_G
Cuts at: 33 271 759 1444 2437 2628 33
Size: 238 488 685 993 191 1121
Fragments arranged by size:
1121 993 685 488 238 191
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 577 738 577
Size: 161 3555
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 239 332 850 1204 239
Size: 93 518 354 2751
Fragments arranged by size:
2751 518 354 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 105 1299 1432 1891 2100 105
Size: 1194 133 459 209 1721
Fragments arranged by size:
1721 1194 459 209 133
TatI w'GTAC_w
Cuts at: 428 512 653 3231 428
Size: 84 141 2578 913
Fragments arranged by size:
2578 913 141 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 3233 3265 622
Size: 32 2579 32 1073
Fragments arranged by size:
2579 1073 32 32
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 575 736 575
Size: 161 3555
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI