Restriction map for
pF1KB6884
(Circular) (Six-base) MAPSORT of: pF1KB6884.seq Check: 5733 from: 1 to: 3716
pF1KB6884 3716 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 375 375
Size: 3716
AccI GT'mk_AC
Cuts at: 754 754
Size: 3716
Acc65I G'GTAC_C
Cuts at: 732 732
Size: 3716
AclI AA'CG_TT
Cuts at: 3556 3556
Size: 3716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 421 622 1541 1973 2720 2988 295
Size: 126 201 919 432 747 268 1023
Fragments arranged by size:
1023 919 747 432 268 201 126
AflIII A'CryG_T
Cuts at: 437 1068 2172 3149 437
Size: 631 1104 977 1004
Fragments arranged by size:
1104 1004 977 631
AgeI A'CCGG_T
Cuts at: 2104 2104
Size: 3716
AleI CACnn'nnGTG
Cuts at: 542 542
Size: 3716
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 343 375 343
Size: 32 3684
AlwNI CAG_nnn'CTG
Cuts at: 399 1021 2588 399
Size: 622 1567 1527
Fragments arranged by size:
1567 1527 622
ApaLI G'TGCA_C
Cuts at: 2486 2486
Size: 3716
ApoI r'AATT_y
Cuts at: 592 720 3069 3325 592
Size: 128 2349 256 983
Fragments arranged by size:
2349 983 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 3716
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
AvaI C'yCGr_G
Cuts at: 346 736 346
Size: 390 3326
BamHI G'GATC_C
Cuts at: 741 741
Size: 3716
BanI G'GyrC_C
Cuts at: 342 732 1146 1394 1429 342
Size: 390 414 248 35 2629
Fragments arranged by size:
2629 414 390 248 35
BanII G_rGCy'C
Cuts at: 730 1760 730
Size: 1030 2686
BbeI G_GCGC'C
Cuts at: 1150 1398 1150
Size: 248 3468
BbsI GAAGACnn'nnnn_
Cuts at: 360 360
Size: 3716
BbvCI CC'TCA_GC
Cuts at: 410 410
Size: 3716
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2375 3716 1614
Size: 761 1341 1614
Fragments arranged by size:
1614 1341 761
BclI T'GATC_A
Cuts at: 1236 1236
Size: 3716
BglI GCCn_nnn'nGGC
Cuts at: 667 667
Size: 3716
BglII A'GATC_T
Cuts at: 1231 3268 1231
Size: 2037 1679
BlpI GC'TnA_GC
Cuts at: 828 828
Size: 3716
Bme1580I G_kGCm'C
Cuts at: 347 539 1341 1434 2490 347
Size: 192 802 93 1056 1573
Fragments arranged by size:
1573 1056 802 192 93
BmrI ACTGGGnnnn_n'
Cuts at: 928 1009 1344 3488 928
Size: 81 335 2144 1156
Fragments arranged by size:
2144 1156 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 3716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 205
Size: 3716
Bpu10I CC'TnA_GC
Cuts at: 410 410
Size: 3716
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 1771 2263 2561 892
Size: 879 492 298 2047
Fragments arranged by size:
2047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3420 3420
Size: 3716
BsaAI yAC'GTr
Cuts at: 1069 1069
Size: 3716
BsaBI GATnn'nnATC
Cuts at: 6 1230 3267 6
Size: 1224 2037 455
Fragments arranged by size:
2037 1224 455
BsaHI Gr'CG_yC
Cuts at: 1147 1395 2097 3593 1147
Size: 248 702 1496 1270
Fragments arranged by size:
1496 1270 702 248
BsaWI w'CCGG_w
Cuts at: 905 1426 2104 2378 2525 2936 905
Size: 521 678 274 147 411 1685
Fragments arranged by size:
1685 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 373 343
Size: 30 3686
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 202 193
Size: 9 3707
BseYI C'CCAG_C
Cuts at: 1141 1899 2476 2972 1141
Size: 758 577 496 1885
Fragments arranged by size:
1885 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 235 505 169
Size: 66 270 3380
Fragments arranged by size:
3380 270 66
BsiEI CG_ry'CG
Cuts at: 104 1258 1304 2512 104
Size: 1154 46 1208 1308
Fragments arranged by size:
1308 1208 1154 46
BsiHKAI G_wGCw'C
Cuts at: 730 1508 1698 2490 730
Size: 778 190 792 1956
Fragments arranged by size:
1956 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 347 539 730 1341 1434 1508 1698 1760 2490
Size: 192 191 611 93 74 190 62 730
Cuts at: 2490 347
Size: 1573
Fragments arranged by size:
1573 730 611 192 191 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2936 2936
Size: 3716
BspHI T'CATG_A
Cuts at: 2892 2892
Size: 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 375 766 980 1282 1663 375
Size: 391 214 302 381 2428
Fragments arranged by size:
2428 391 381 302 214
BsrBI CCG'CTC
Cuts at: 50 192 2008 2062 3546 3711 50
Size: 142 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 165 142 55 54
BsrDI GCAATG_nn'
Cuts at: 1628 1628
Size: 3716
BsrFI r'CCGG_y
Cuts at: 333 670 1714 1895 2104 333
Size: 337 1044 181 209 1945
Fragments arranged by size:
1945 1044 337 209 181
BssSI C'ACGA_G
Cuts at: 1987 2345 1987
Size: 358 3358
BstAPI GCAn_nnn'nTGC
Cuts at: 1093 1093
Size: 3716
BstBI TT'CG_AA
Cuts at: 2077 2077
Size: 3716
BstYI r'GATC_y
Cuts at: 1 549 741 1208 1231 1566 1812 2824 2910
Size: 548 192 467 23 335 246 1012 86
Cuts at: 2910 2922 3268 1
Size: 12 346 449
Fragments arranged by size:
1012 548 467 449 346 335 246 192
86 23 12
BtgI C'CryG_G
Cuts at: 107 246 362 543 107
Size: 139 116 181 3280
Fragments arranged by size:
3280 181 139 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1666 1847 2010 1666
Size: 181 163 3372
Fragments arranged by size:
3372 181 163
BtsI GCAGTG_nn'
Cuts at: 223 488 524 959 223
Size: 265 36 435 2980
Fragments arranged by size:
2980 435 265 36
DraI TTT'AAA
Cuts at: 715 715
Size: 3716
DrdI GACnn_nn'nnGTC
Cuts at: 421 1422 2280 421
Size: 1001 858 1857
Fragments arranged by size:
1857 1001 858
EaeI y'GGCC_r
Cuts at: 110 1301 1475 1866 1893 3238 110
Size: 1191 174 391 27 1345 588
Fragments arranged by size:
1345 1191 588 391 174 27
EagI C'GGCC_G
Cuts at: 1301 1301
Size: 3716
EarI CTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1924 2234 2380 3526 1924
Size: 310 146 1146 2114
Fragments arranged by size:
2114 1146 310 146
EcoICRI GAG'CTC
Cuts at: 728 728
Size: 3716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 295 421 622 1541 1973 2720 2988 205
Size: 90 126 201 919 432 747 268 933
Fragments arranged by size:
933 919 747 432 268 201 126 90
EcoO109I rG'GnC_Cy
Cuts at: 121 491 659 855 121
Size: 370 168 196 2982
Fragments arranged by size:
2982 370 196 168
EcoRI G'AATT_C
Cuts at: 592 720 592
Size: 128 3588
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1735 1735
Size: 3716
FspI TGC'GCA
Cuts at: 1497 1497
Size: 3716
HaeII r_GCGC'y
Cuts at: 255 1150 1398 2420 3399 255
Size: 895 248 1022 979 572
Fragments arranged by size:
1022 979 895 572 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1558 1588 1590 1620 1558
Size: 30 2 30 3654
Fragments arranged by size:
3654 30 30 2
HincII GTy'rAC
Cuts at: 453 755 453
Size: 302 3414
KasI G'GCGC_C
Cuts at: 1146 1394 1146
Size: 248 3468
KpnI G_GTAC'C
Cuts at: 736 736
Size: 3716
MluI A'CGCG_T
Cuts at: 3149 3149
Size: 3716
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2387 2571 2387
Size: 184 3532
MscI TGG'CCA
Cuts at: 112 1477 112
Size: 1365 2351
MslI CAynn'nnrTG
Cuts at: 542 1705 1832 542
Size: 1163 127 2426
Fragments arranged by size:
2426 1163 127
MspA1I CmG'CkG
Cuts at: 609 827 1141 1501 2514 2759 609
Size: 218 314 360 1013 245 1566
Fragments arranged by size:
1566 1013 360 314 245 218
NaeI GCC'GGC
Cuts at: 672 1897 672
Size: 1225 2491
NarI GG'CG_CC
Cuts at: 1147 1395 1147
Size: 248 3468
NcoI C'CATG_G
Cuts at: 107 362 107
Size: 255 3461
NgoMIV G'CCGG_C
Cuts at: 670 1895 670
Size: 1225 2491
NspI r_CATG'y
Cuts at: 441 628 769 1269 2176 441
Size: 187 141 500 907 1981
Fragments arranged by size:
1981 907 500 187 141
PciI A'CATG_T
Cuts at: 437 2172 437
Size: 1735 1981
PflMI CCAn_nnn'nTGG
Cuts at: 247 247
Size: 3716
PmeI GTTT'AAAC
Cuts at: 715 715
Size: 3716
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 343 375 2855 2887 343
Size: 32 2480 32 1172
Fragments arranged by size:
2480 1172 32 32
PpuMI rG'GwC_Cy
Cuts at: 121 491 121
Size: 370 3346
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 298 330 298
Size: 32 3684
PstI C_TGCA'G
Cuts at: 614 763 1448 614
Size: 149 685 2882
Fragments arranged by size:
2882 685 149
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3716
PvuII CAG'CTG
Cuts at: 609 1141 1501 609
Size: 532 360 2824
Fragments arranged by size:
2824 532 360
RsrII CG'GwC_CG
Cuts at: 1911 1911
Size: 3716
SacI G_AGCT'C
Cuts at: 730 730
Size: 3716
SalI G'TCGA_C
Cuts at: 753 753
Size: 3716
SapI GCTCTTCn'nnn_
Cuts at: 1739 1949 1739
Size: 210 3506
SbfI CC_TGCA'GG
Cuts at: 763 763
Size: 3716
ScaI AGT'ACT
Cuts at: 3233 3233
Size: 3716
SfcI C'TryA_G
Cuts at: 33 610 759 1444 2437 2628 33
Size: 577 149 685 993 191 1121
Fragments arranged by size:
1121 993 685 577 191 149
SfiI GGCCn_nnn'nGGCC
Cuts at: 667 667
Size: 3716
SfoI GGC'GCC
Cuts at: 1148 1396 1148
Size: 248 3468
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3716
SmaI CCC'GGG
Cuts at: 738 738
Size: 3716
SmlI C'TyrA_G
Cuts at: 871 1786 2278 2540 871
Size: 915 492 262 2047
Fragments arranged by size:
2047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3716
SphI G_CATG'C
Cuts at: 769 1269 769
Size: 500 3216
StyI C'CwwG_G
Cuts at: 107 362 850 1204 107
Size: 255 488 354 2619
Fragments arranged by size:
2619 488 354 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 1432 1891 2100 1299
Size: 133 459 209 2915
Fragments arranged by size:
2915 459 209 133
TatI w'GTAC_w
Cuts at: 3231 3231
Size: 3716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 3684
Tth111I GACn'n_nGTC
Cuts at: 1513 1513
Size: 3716
XbaI T'CTAG_A
Cuts at: 747 747
Size: 3716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1211 1211
Size: 3716
XmaI C'CCGG_G
Cuts at: 736 736
Size: 3716
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI