Restriction map for
pF1KB6885
(Circular) (Six-base) MAPSORT of: pF1KB6885.seq Check: 9802 from: 1 to: 3653
pF1KB6885 3653 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 391 691 391
Size: 300 3353
Acc65I G'GTAC_C
Cuts at: 669 669
Size: 3653
AclI AA'CG_TT
Cuts at: 3493 3493
Size: 3653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 1478 1910 2657 2925 246
Size: 1232 432 747 268 974
Fragments arranged by size:
1232 974 747 432 268
AflIII A'CryG_T
Cuts at: 386 1005 2109 3086 386
Size: 619 1104 977 953
Fragments arranged by size:
1104 977 953 619
AgeI A'CCGG_T
Cuts at: 2041 2041
Size: 3653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3653
AlwNI CAG_nnn'CTG
Cuts at: 483 958 2525 483
Size: 475 1567 1611
Fragments arranged by size:
1611 1567 475
ApaLI G'TGCA_C
Cuts at: 2423 2423
Size: 3653
ApoI r'AATT_y
Cuts at: 657 3006 3262 657
Size: 2349 256 1048
Fragments arranged by size:
2349 1048 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3653
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
AvaI C'yCGr_G
Cuts at: 544 673 544
Size: 129 3524
BamHI G'GATC_C
Cuts at: 678 678
Size: 3653
BanI G'GyrC_C
Cuts at: 616 669 1083 1331 1366 616
Size: 53 414 248 35 2903
Fragments arranged by size:
2903 414 248 53 35
BanII G_rGCy'C
Cuts at: 545 667 1697 545
Size: 122 1030 2501
Fragments arranged by size:
2501 1030 122
BbeI G_GCGC'C
Cuts at: 620 1087 1335 620
Size: 467 248 2938
Fragments arranged by size:
2938 467 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 120 154 120
Size: 34 3619
BciVI GTATCCnnnnn_n'
Cuts at: 1551 2312 3653 1551
Size: 761 1341 1551
Fragments arranged by size:
1551 1341 761
BclI T'GATC_A
Cuts at: 1173 1173
Size: 3653
BglII A'GATC_T
Cuts at: 1168 3205 1168
Size: 2037 1616
BlpI GC'TnA_GC
Cuts at: 765 765
Size: 3653
Bme1580I G_kGCm'C
Cuts at: 516 1278 1371 2427 516
Size: 762 93 1056 1742
Fragments arranged by size:
1742 1056 762 93
BmgBI CAC'GTC
Cuts at: 387 387
Size: 3653
BmrI ACTGGGnnnn_n'
Cuts at: 185 865 946 1281 3425 185
Size: 680 81 335 2144 413
Fragments arranged by size:
2144 680 413 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2020 2020
Size: 3653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 367 214
Size: 153 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 1708 2200 2498 829
Size: 879 492 298 1984
Fragments arranged by size:
1984 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 190 3357 190
Size: 3167 486
BsaAI yAC'GTr
Cuts at: 528 1006 528
Size: 478 3175
BsaBI GATnn'nnATC
Cuts at: 6 1167 3204 6
Size: 1161 2037 455
Fragments arranged by size:
2037 1161 455
BsaHI Gr'CG_yC
Cuts at: 617 1084 1332 2034 3530 617
Size: 467 248 702 1496 740
Fragments arranged by size:
1496 740 702 467 248
BsaWI w'CCGG_w
Cuts at: 411 842 1363 2041 2315 2462 2873 411
Size: 431 521 678 274 147 411 1191
Fragments arranged by size:
1191 678 521 431 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 3653
BseYI C'CCAG_C
Cuts at: 476 1078 1836 2413 2909 476
Size: 602 758 577 496 1220
Fragments arranged by size:
1220 758 602 577 496
BsiEI CG_ry'CG
Cuts at: 104 404 1195 1241 2449 104
Size: 300 791 46 1208 1308
Fragments arranged by size:
1308 1208 791 300 46
BsiHKAI G_wGCw'C
Cuts at: 533 545 667 1445 1635 2427 533
Size: 12 122 778 190 792 1759
Fragments arranged by size:
1759 792 778 190 122 12
BsmBI CGTCTCn'nnnn_
Cuts at: 343 343
Size: 3653
Bsp1286I G_dGCh'C
Cuts at: 516 533 545 667 1278 1371 1445 1635 1697
Size: 17 12 122 611 93 74 190 62
Cuts at: 1697 2427 516
Size: 730 1742
Fragments arranged by size:
1742 730 611 190 122 93 74 62
17 12
BspEI T'CCGG_A
Cuts at: 411 2873 411
Size: 2462 1191
BspHI T'CATG_A
Cuts at: 2829 2829
Size: 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 917 1219 1600 703
Size: 214 302 381 2756
Fragments arranged by size:
2756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1945 1999 3483 3648 50
Size: 1895 54 1484 165 55
Fragments arranged by size:
1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 1565 173
Size: 1392 2261
BsrFI r'CCGG_y
Cuts at: 1651 1832 2041 1651
Size: 181 209 3263
Fragments arranged by size:
3263 209 181
BssSI C'ACGA_G
Cuts at: 165 1924 2282 165
Size: 1759 358 1536
Fragments arranged by size:
1759 1536 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1030
Size: 3653
BstBI TT'CG_AA
Cuts at: 2014 2014
Size: 3653
BstYI r'GATC_y
Cuts at: 1 678 1145 1168 1503 1749 2761 2847 2859
Size: 677 467 23 335 246 1012 86 12
Cuts at: 2859 3205 1
Size: 346 449
Fragments arranged by size:
1012 677 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 249 297 434 609 107
Size: 142 48 137 175 3151
Fragments arranged by size:
3151 175 142 137 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1603 1784 1947 1603
Size: 181 163 3309
Fragments arranged by size:
3309 181 163
BtsI GCAGTG_nn'
Cuts at: 896 896
Size: 3653
DraI TTT'AAA
Cuts at: 652 652
Size: 3653
DrdI GACnn_nn'nnGTC
Cuts at: 1359 2217 1359
Size: 858 2795
EaeI y'GGCC_r
Cuts at: 632 1238 1412 1803 1830 3175 632
Size: 606 174 391 27 1345 1110
Fragments arranged by size:
1345 1110 606 391 174 27
EagI C'GGCC_G
Cuts at: 1238 1238
Size: 3653
EarI CTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1861 2171 2317 3463 1861
Size: 310 146 1146 2051
Fragments arranged by size:
2051 1146 310 146
EcoICRI GAG'CTC
Cuts at: 543 665 543
Size: 122 3531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 246 367 1478 1910 2657 2925 214
Size: 32 121 1111 432 747 268 942
Fragments arranged by size:
1111 942 747 432 268 121 32
EcoO109I rG'GnC_Cy
Cuts at: 492 792 492
Size: 300 3353
EcoRI G'AATT_C
Cuts at: 657 657
Size: 3653
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1672 1672
Size: 3653
FspI TGC'GCA
Cuts at: 487 1434 487
Size: 947 2706
HaeII r_GCGC'y
Cuts at: 483 620 1087 1335 2357 3336 483
Size: 137 467 248 1022 979 800
Fragments arranged by size:
1022 979 800 467 248 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1495 1525 1527 1557 1495
Size: 30 2 30 3591
Fragments arranged by size:
3591 30 30 2
HincII GTy'rAC
Cuts at: 392 692 392
Size: 300 3353
KasI G'GCGC_C
Cuts at: 616 1083 1331 616
Size: 467 248 2938
Fragments arranged by size:
2938 467 248
KpnI G_GTAC'C
Cuts at: 673 673
Size: 3653
MluI A'CGCG_T
Cuts at: 3086 3086
Size: 3653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 424 2324 2508 424
Size: 1900 184 1569
Fragments arranged by size:
1900 1569 184
MscI TGG'CCA
Cuts at: 634 1414 634
Size: 780 2873
MslI CAynn'nnrTG
Cuts at: 1642 1769 1642
Size: 127 3526
MspA1I CmG'CkG
Cuts at: 251 329 764 1078 1438 2451 2696 251
Size: 78 435 314 360 1013 245 1208
Fragments arranged by size:
1208 1013 435 360 314 245 78
NaeI GCC'GGC
Cuts at: 1834 1834
Size: 3653
NarI GG'CG_CC
Cuts at: 617 1084 1332 617
Size: 467 248 2938
Fragments arranged by size:
2938 467 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3653
NgoMIV G'CCGG_C
Cuts at: 1832 1832
Size: 3653
NspI r_CATG'y
Cuts at: 706 1206 2113 706
Size: 500 907 2246
Fragments arranged by size:
2246 907 500
PciI A'CATG_T
Cuts at: 2109 2109
Size: 3653
PfoI T'CCnGG_A
Cuts at: 344 344
Size: 3653
PmeI GTTT'AAAC
Cuts at: 652 652
Size: 3653
PmlI CAC'GTG
Cuts at: 528 528
Size: 3653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2792 2824 2792
Size: 32 3621
PspXI vC'TCGA_Gb
Cuts at: 544 544
Size: 3653
PstI C_TGCA'G
Cuts at: 700 1385 700
Size: 685 2968
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3653
PvuII CAG'CTG
Cuts at: 329 1078 1438 329
Size: 749 360 2544
Fragments arranged by size:
2544 749 360
RsrII CG'GwC_CG
Cuts at: 1848 1848
Size: 3653
SacI G_AGCT'C
Cuts at: 545 667 545
Size: 122 3531
SacII CC_GC'GG
Cuts at: 252 252
Size: 3653
SalI G'TCGA_C
Cuts at: 390 690 390
Size: 300 3353
SapI GCTCTTCn'nnn_
Cuts at: 1676 1886 1676
Size: 210 3443
SbfI CC_TGCA'GG
Cuts at: 700 700
Size: 3653
ScaI AGT'ACT
Cuts at: 3170 3170
Size: 3653
SfcI C'TryA_G
Cuts at: 33 696 1381 2374 2565 33
Size: 663 685 993 191 1121
Fragments arranged by size:
1121 993 685 663 191
SfoI GGC'GCC
Cuts at: 618 1085 1333 618
Size: 467 248 2938
Fragments arranged by size:
2938 467 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3653
SmaI CCC'GGG
Cuts at: 675 675
Size: 3653
SmlI C'TyrA_G
Cuts at: 544 808 1723 2215 2477 544
Size: 264 915 492 262 1720
Fragments arranged by size:
1720 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3653
SphI G_CATG'C
Cuts at: 706 1206 706
Size: 500 3153
StyI C'CwwG_G
Cuts at: 107 536 787 1141 107
Size: 429 251 354 2619
Fragments arranged by size:
2619 429 354 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 490 610 1236 1369 1828 2037 490
Size: 120 626 133 459 209 2106
Fragments arranged by size:
2106 626 459 209 133 120
TatI w'GTAC_w
Cuts at: 3168 3168
Size: 3653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3621
Tth111I GACn'n_nGTC
Cuts at: 1450 1450
Size: 3653
XbaI T'CTAG_A
Cuts at: 684 684
Size: 3653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 238 432 1148 238
Size: 194 716 2743
Fragments arranged by size:
2743 716 194
XhoI C'TCGA_G
Cuts at: 544 544
Size: 3653
XmaI C'CCGG_G
Cuts at: 673 673
Size: 3653
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PpuMI PshAI PsiI
PspOMI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI