Restriction map for
pF1KB6886
(Circular) (Six-base) MAPSORT of: pF1KB6886.seq Check: 5903 from: 1 to: 3686
pF1KB6886 3686 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 724 724
Size: 3686
Acc65I G'GTAC_C
Cuts at: 702 702
Size: 3686
AclI AA'CG_TT
Cuts at: 3526 3526
Size: 3686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 670 1511 1943 2690 2958 357
Size: 313 841 432 747 268 1085
Fragments arranged by size:
1085 841 747 432 313 268
AflIII A'CryG_T
Cuts at: 1038 2142 3119 1038
Size: 1104 977 1605
Fragments arranged by size:
1605 1104 977
AgeI A'CCGG_T
Cuts at: 2074 2074
Size: 3686
AlwNI CAG_nnn'CTG
Cuts at: 192 400 991 2558 192
Size: 208 591 1567 1320
Fragments arranged by size:
1567 1320 591 208
ApaLI G'TGCA_C
Cuts at: 2456 2456
Size: 3686
ApoI r'AATT_y
Cuts at: 210 690 3039 3295 210
Size: 480 2349 256 601
Fragments arranged by size:
2349 601 480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
AvaI C'yCGr_G
Cuts at: 706 706
Size: 3686
BamHI G'GATC_C
Cuts at: 711 711
Size: 3686
BanI G'GyrC_C
Cuts at: 129 702 1116 1364 1399 129
Size: 573 414 248 35 2416
Fragments arranged by size:
2416 573 414 248 35
BanII G_rGCy'C
Cuts at: 173 512 700 1730 173
Size: 339 188 1030 2129
Fragments arranged by size:
2129 1030 339 188
BbeI G_GCGC'C
Cuts at: 133 1120 1368 133
Size: 987 248 2451
Fragments arranged by size:
2451 987 248
BbsI GAAGACnn'nnnn_
Cuts at: 260 260
Size: 3686
BbvCI CC'TCA_GC
Cuts at: 393 393
Size: 3686
BciVI GTATCCnnnnn_n'
Cuts at: 1584 2345 3686 1584
Size: 761 1341 1584
Fragments arranged by size:
1584 1341 761
BclI T'GATC_A
Cuts at: 1206 1206
Size: 3686
BglII A'GATC_T
Cuts at: 224 1201 3238 224
Size: 977 2037 672
Fragments arranged by size:
2037 977 672
BlpI GC'TnA_GC
Cuts at: 362 798 362
Size: 436 3250
Bme1580I G_kGCm'C
Cuts at: 1311 1404 2460 1311
Size: 93 1056 2537
Fragments arranged by size:
2537 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 898 979 1314 3458 898
Size: 81 335 2144 1126
Fragments arranged by size:
2144 1126 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 3686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 467
Size: 3686
Bpu10I CC'TnA_GC
Cuts at: 393 393
Size: 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 862 1741 2233 2531 862
Size: 879 492 298 2017
Fragments arranged by size:
2017 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3390 3390
Size: 3686
BsaAI yAC'GTr
Cuts at: 1039 1039
Size: 3686
BsaBI GATnn'nnATC
Cuts at: 6 296 1200 3237 6
Size: 290 904 2037 455
Fragments arranged by size:
2037 904 455 290
BsaHI Gr'CG_yC
Cuts at: 130 1117 1365 2067 3563 130
Size: 987 248 702 1496 253
Fragments arranged by size:
1496 987 702 253 248
BsaWI w'CCGG_w
Cuts at: 344 875 1396 2074 2348 2495 2906 344
Size: 531 521 678 274 147 411 1124
Fragments arranged by size:
1124 678 531 521 411 274 147
BseYI C'CCAG_C
Cuts at: 287 511 1111 1869 2446 2942 287
Size: 224 600 758 577 496 1031
Fragments arranged by size:
1031 758 600 577 496 224
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 568
Size: 3686
BsiEI CG_ry'CG
Cuts at: 104 1228 1274 2482 104
Size: 1124 46 1208 1308
Fragments arranged by size:
1308 1208 1124 46
BsiHKAI G_wGCw'C
Cuts at: 173 700 1478 1668 2460 173
Size: 527 778 190 792 1399
Fragments arranged by size:
1399 792 778 527 190
Bsp1286I G_dGCh'C
Cuts at: 173 512 700 1311 1404 1478 1668 1730 2460
Size: 339 188 611 93 74 190 62 730
Cuts at: 2460 173
Size: 1399
Fragments arranged by size:
1399 730 611 339 190 188 93 74
62
BspEI T'CCGG_A
Cuts at: 2906 2906
Size: 3686
BspHI T'CATG_A
Cuts at: 2862 2862
Size: 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 950 1252 1633 736
Size: 214 302 381 2789
Fragments arranged by size:
2789 381 302 214
BsrBI CCG'CTC
Cuts at: 50 366 1978 2032 3516 3681 50
Size: 316 1612 54 1484 165 55
Fragments arranged by size:
1612 1484 316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1598 1598
Size: 3686
BsrFI r'CCGG_y
Cuts at: 632 1684 1865 2074 632
Size: 1052 181 209 2244
Fragments arranged by size:
2244 1052 209 181
BssSI C'ACGA_G
Cuts at: 172 668 1957 2315 172
Size: 496 1289 358 1543
Fragments arranged by size:
1543 1289 496 358
BstAPI GCAn_nnn'nTGC
Cuts at: 409 1063 409
Size: 654 3032
BstBI TT'CG_AA
Cuts at: 2047 2047
Size: 3686
BstEII G'GTnAC_C
Cuts at: 387 387
Size: 3686
BstYI r'GATC_y
Cuts at: 1 140 224 531 541 711 1178 1201 1536
Size: 139 84 307 10 170 467 23 335
Cuts at: 1536 1782 2794 2880 2892 3238 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 307 246 170
139 86 84 23 12 10
Bsu36I CC'TnA_GG
Cuts at: 527 588 527
Size: 61 3625
BtgI C'CryG_G
Cuts at: 107 107
Size: 3686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 1636 1817 1980 127
Size: 1509 181 163 1833
Fragments arranged by size:
1833 1509 181 163
BtsI GCAGTG_nn'
Cuts at: 929 929
Size: 3686
DraI TTT'AAA
Cuts at: 685 685
Size: 3686
DrdI GACnn_nn'nnGTC
Cuts at: 1392 2250 1392
Size: 858 2828
EaeI y'GGCC_r
Cuts at: 1271 1445 1836 1863 3208 1271
Size: 174 391 27 1345 1749
Fragments arranged by size:
1749 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1271 1271
Size: 3686
EarI CTCTTCn'nnn_
Cuts at: 376 1709 1919 376
Size: 1333 210 2143
Fragments arranged by size:
2143 1333 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 211 1894 2204 2350 3496 181
Size: 30 1683 310 146 1146 371
Fragments arranged by size:
1683 1146 371 310 146 30
EcoICRI GAG'CTC
Cuts at: 171 698 171
Size: 527 3159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 467 670 1511 1943 2690 2958 357
Size: 110 203 841 432 747 268 1085
Fragments arranged by size:
1085 841 747 432 268 203 110
EcoO109I rG'GnC_Cy
Cuts at: 522 825 522
Size: 303 3383
EcoRI G'AATT_C
Cuts at: 690 690
Size: 3686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 494 1705 494
Size: 1211 2475
FspI TGC'GCA
Cuts at: 1467 1467
Size: 3686
HaeII r_GCGC'y
Cuts at: 133 1120 1368 2390 3369 133
Size: 987 248 1022 979 450
Fragments arranged by size:
1022 987 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1528 1558 1560 1590 1528
Size: 30 2 30 3624
Fragments arranged by size:
3624 30 30 2
HincII GTy'rAC
Cuts at: 725 725
Size: 3686
KasI G'GCGC_C
Cuts at: 129 1116 1364 129
Size: 987 248 2451
Fragments arranged by size:
2451 987 248
KpnI G_GTAC'C
Cuts at: 706 706
Size: 3686
MluI A'CGCG_T
Cuts at: 3119 3119
Size: 3686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2357 2541 2357
Size: 184 3502
MscI TGG'CCA
Cuts at: 1447 1447
Size: 3686
MslI CAynn'nnrTG
Cuts at: 112 262 1675 1802 112
Size: 150 1413 127 1996
Fragments arranged by size:
1996 1413 150 127
MspA1I CmG'CkG
Cuts at: 400 797 1111 1471 2484 2729 400
Size: 397 314 360 1013 245 1357
Fragments arranged by size:
1357 1013 397 360 314 245
NaeI GCC'GGC
Cuts at: 1867 1867
Size: 3686
NarI GG'CG_CC
Cuts at: 130 1117 1365 130
Size: 987 248 2451
Fragments arranged by size:
2451 987 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3686
NgoMIV G'CCGG_C
Cuts at: 1865 1865
Size: 3686
NspI r_CATG'y
Cuts at: 739 1239 2146 739
Size: 500 907 2279
Fragments arranged by size:
2279 907 500
PciI A'CATG_T
Cuts at: 2142 2142
Size: 3686
PflMI CCAn_nnn'nTGG
Cuts at: 460 460
Size: 3686
PfoI T'CCnGG_A
Cuts at: 143 143
Size: 3686
PmeI GTTT'AAAC
Cuts at: 685 685
Size: 3686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 2825
Size: 32 3654
PpuMI rG'GwC_Cy
Cuts at: 522 522
Size: 3686
PstI C_TGCA'G
Cuts at: 405 733 1418 405
Size: 328 685 2673
Fragments arranged by size:
2673 685 328
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3686
PvuII CAG'CTG
Cuts at: 400 1111 1471 400
Size: 711 360 2615
Fragments arranged by size:
2615 711 360
RsrII CG'GwC_CG
Cuts at: 1881 1881
Size: 3686
SacI G_AGCT'C
Cuts at: 173 700 173
Size: 527 3159
SalI G'TCGA_C
Cuts at: 723 723
Size: 3686
SapI GCTCTTCn'nnn_
Cuts at: 376 1709 1919 376
Size: 1333 210 2143
Fragments arranged by size:
2143 1333 210
SbfI CC_TGCA'GG
Cuts at: 733 733
Size: 3686
ScaI AGT'ACT
Cuts at: 3203 3203
Size: 3686
SfcI C'TryA_G
Cuts at: 33 401 729 1414 2407 2598 33
Size: 368 328 685 993 191 1121
Fragments arranged by size:
1121 993 685 368 328 191
SfoI GGC'GCC
Cuts at: 131 1118 1366 131
Size: 987 248 2451
Fragments arranged by size:
2451 987 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3686
SmaI CCC'GGG
Cuts at: 708 708
Size: 3686
SmlI C'TyrA_G
Cuts at: 841 1756 2248 2510 841
Size: 915 492 262 2017
Fragments arranged by size:
2017 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3686
SphI G_CATG'C
Cuts at: 739 1239 739
Size: 500 3186
SspI AAT'ATT
Cuts at: 276 276
Size: 3686
StyI C'CwwG_G
Cuts at: 107 820 1174 107
Size: 713 354 2619
Fragments arranged by size:
2619 713 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1269 1402 1861 2070 1269
Size: 133 459 209 2885
Fragments arranged by size:
2885 459 209 133
TatI w'GTAC_w
Cuts at: 3201 3201
Size: 3686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 456 488 3203 3235 456
Size: 32 2715 32 907
Fragments arranged by size:
2715 907 32 32
Tth111I GACn'n_nGTC
Cuts at: 1483 1483
Size: 3686
XbaI T'CTAG_A
Cuts at: 717 717
Size: 3686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1181 1181
Size: 3686
XmaI C'CCGG_G
Cuts at: 706 706
Size: 3686
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI XmnI ZraI