(Circular) (Six-base) MAPSORT of: pF1KB6887.seq Check: 2086 from: 1 to: 3662 pF1KB6887 3662 bp With 182 enzymes: SgfI * June 26, 2008 10:48 .. AccI GT'mk_AC Cuts at: 700 700 Size: 3662 Acc65I G'GTAC_C Cuts at: 678 678 Size: 3662 AclI AA'CG_TT Cuts at: 3502 3502 Size: 3662 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1487 1919 2666 2934 1487 Size: 432 747 268 2215 Fragments arranged by size: 2215 747 432 268 AflIII A'CryG_T Cuts at: 1014 2118 3095 1014 Size: 1104 977 1581 Fragments arranged by size: 1581 1104 977 AgeI A'CCGG_T Cuts at: 2050 2050 Size: 3662 AlwNI CAG_nnn'CTG Cuts at: 382 644 967 2534 382 Size: 262 323 1567 1510 Fragments arranged by size: 1567 1510 323 262 ApaLI G'TGCA_C Cuts at: 417 2432 417 Size: 2015 1647 ApoI r'AATT_y Cuts at: 666 3015 3271 666 Size: 2349 256 1057 Fragments arranged by size: 2349 1057 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3662 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3662 AvaI C'yCGr_G Cuts at: 682 682 Size: 3662 BamHI G'GATC_C Cuts at: 687 687 Size: 3662 BanI G'GyrC_C Cuts at: 678 1092 1340 1375 678 Size: 414 248 35 2965 Fragments arranged by size: 2965 414 248 35 BanII G_rGCy'C Cuts at: 374 548 676 1706 374 Size: 174 128 1030 2330 Fragments arranged by size: 2330 1030 174 128 BbeI G_GCGC'C Cuts at: 1096 1344 1096 Size: 248 3414 BciVI GTATCCnnnnn_n' Cuts at: 1560 2321 3662 1560 Size: 761 1341 1560 Fragments arranged by size: 1560 1341 761 BclI T'GATC_A Cuts at: 1182 1182 Size: 3662 BglII A'GATC_T Cuts at: 512 1177 3214 512 Size: 665 2037 960 Fragments arranged by size: 2037 960 665 BlpI GC'TnA_GC Cuts at: 774 774 Size: 3662 Bme1580I G_kGCm'C Cuts at: 421 537 1287 1380 2436 421 Size: 116 750 93 1056 1647 Fragments arranged by size: 1647 1056 750 116 93 BmgBI CAC'GTC Cuts at: 204 204 Size: 3662 BmrI ACTGGGnnnn_n' Cuts at: 874 955 1290 3434 874 Size: 81 335 2144 1102 Fragments arranged by size: 2144 1102 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 577 2029 577 Size: 1452 2210 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 388 388 Size: 3662 Bpu10I CC'TnA_GC Cuts at: 158 158 Size: 3662 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 838 1717 2209 2507 838 Size: 879 492 298 1993 Fragments arranged by size: 1993 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3366 3366 Size: 3662 BsaAI yAC'GTr Cuts at: 1015 1015 Size: 3662 BsaBI GATnn'nnATC Cuts at: 6 1176 3213 6 Size: 1170 2037 455 Fragments arranged by size: 2037 1170 455 BsaHI Gr'CG_yC Cuts at: 1093 1341 2043 3539 1093 Size: 248 702 1496 1216 Fragments arranged by size: 1496 1216 702 248 BsaWI w'CCGG_w Cuts at: 851 1372 2050 2324 2471 2882 851 Size: 521 678 274 147 411 1631 Fragments arranged by size: 1631 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 562 592 562 Size: 30 3632 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 260 538 556 260 Size: 278 18 3366 Fragments arranged by size: 3366 278 18 BseYI C'CCAG_C Cuts at: 305 333 1087 1845 2422 2918 305 Size: 28 754 758 577 496 1049 Fragments arranged by size: 1049 758 754 577 496 28 BsiEI CG_ry'CG Cuts at: 104 1204 1250 2458 104 Size: 1100 46 1208 1308 Fragments arranged by size: 1308 1208 1100 46 BsiHKAI G_wGCw'C Cuts at: 421 548 676 1454 1644 2436 421 Size: 127 128 778 190 792 1647 Fragments arranged by size: 1647 792 778 190 128 127 Bsp1286I G_dGCh'C Cuts at: 374 421 537 548 676 1287 1380 1454 1644 Size: 47 116 11 128 611 93 74 190 Cuts at: 1644 1706 2436 374 Size: 62 730 1600 Fragments arranged by size: 1600 730 611 190 128 116 93 74 62 47 11 BspEI T'CCGG_A Cuts at: 2882 2882 Size: 3662 BspHI T'CATG_A Cuts at: 2838 2838 Size: 3662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 712 926 1228 1609 712 Size: 214 302 381 2765 Fragments arranged by size: 2765 381 302 214 BsrBI CCG'CTC Cuts at: 50 1954 2008 3492 3657 50 Size: 1904 54 1484 165 55 Fragments arranged by size: 1904 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1574 1574 Size: 3662 BsrFI r'CCGG_y Cuts at: 1660 1841 2050 1660 Size: 181 209 3272 Fragments arranged by size: 3272 209 181 BssSI C'ACGA_G Cuts at: 1933 2291 1933 Size: 358 3304 BstAPI GCAn_nnn'nTGC Cuts at: 1039 1039 Size: 3662 BstBI TT'CG_AA Cuts at: 2023 2023 Size: 3662 BstYI r'GATC_y Cuts at: 1 512 687 1154 1177 1512 1758 2770 2856 Size: 511 175 467 23 335 246 1012 86 Cuts at: 2856 2868 3214 1 Size: 12 346 449 Fragments arranged by size: 1012 511 467 449 346 335 246 175 86 23 12 BtgI C'CryG_G Cuts at: 620 620 Size: 3662 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1612 1793 1956 1612 Size: 181 163 3318 Fragments arranged by size: 3318 181 163 BtsI GCAGTG_nn' Cuts at: 905 905 Size: 3662 DraI TTT'AAA Cuts at: 661 661 Size: 3662 DrdI GACnn_nn'nnGTC Cuts at: 1368 2226 1368 Size: 858 2804 EaeI y'GGCC_r Cuts at: 596 1247 1421 1812 1839 3184 596 Size: 651 174 391 27 1345 1074 Fragments arranged by size: 1345 1074 651 391 174 27 EagI C'GGCC_G Cuts at: 1247 1247 Size: 3662 EarI CTCTTCn'nnn_ Cuts at: 130 1685 1895 130 Size: 1555 210 1897 Fragments arranged by size: 1897 1555 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 475 1870 2180 2326 3472 475 Size: 1395 310 146 1146 665 Fragments arranged by size: 1395 1146 665 310 146 EcoICRI GAG'CTC Cuts at: 546 674 546 Size: 128 3534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 388 1487 1919 2666 2934 388 Size: 1099 432 747 268 1116 Fragments arranged by size: 1116 1099 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 636 636 Size: 3662 EcoO109I rG'GnC_Cy Cuts at: 801 801 Size: 3662 EcoRI G'AATT_C Cuts at: 666 666 Size: 3662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1681 1681 Size: 3662 FspI TGC'GCA Cuts at: 1443 1443 Size: 3662 HaeII r_GCGC'y Cuts at: 1096 1344 2366 3345 1096 Size: 248 1022 979 1413 Fragments arranged by size: 1413 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 562 594 1504 1534 1536 1566 562 Size: 32 910 30 2 30 2658 Fragments arranged by size: 2658 910 32 30 30 2 HincII GTy'rAC Cuts at: 701 701 Size: 3662 KasI G'GCGC_C Cuts at: 1092 1340 1092 Size: 248 3414 KpnI G_GTAC'C Cuts at: 682 682 Size: 3662 MluI A'CGCG_T Cuts at: 3095 3095 Size: 3662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 342 2333 2517 342 Size: 1991 184 1487 Fragments arranged by size: 1991 1487 184 MscI TGG'CCA Cuts at: 598 1423 598 Size: 825 2837 MslI CAynn'nnrTG Cuts at: 1651 1778 1651 Size: 127 3535 MspA1I CmG'CkG Cuts at: 183 605 773 1087 1447 2460 2705 183 Size: 422 168 314 360 1013 245 1140 Fragments arranged by size: 1140 1013 422 360 314 245 168 NaeI GCC'GGC Cuts at: 1843 1843 Size: 3662 NarI GG'CG_CC Cuts at: 1093 1341 1093 Size: 248 3414 NgoMIV G'CCGG_C Cuts at: 1841 1841 Size: 3662 NspI r_CATG'y Cuts at: 715 1215 2122 715 Size: 500 907 2255 Fragments arranged by size: 2255 907 500 PciI A'CATG_T Cuts at: 2118 2118 Size: 3662 PflMI CCAn_nnn'nTGG Cuts at: 621 621 Size: 3662 PmeI GTTT'AAAC Cuts at: 661 661 Size: 3662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 274 306 2801 2833 274 Size: 32 2495 32 1103 Fragments arranged by size: 2495 1103 32 32 PshAI GACnn'nnGTC Cuts at: 563 563 Size: 3662 PstI C_TGCA'G Cuts at: 147 709 1394 147 Size: 562 685 2415 Fragments arranged by size: 2415 685 562 PvuI CG_AT'CG Cuts at: 104 104 Size: 3662 PvuII CAG'CTG Cuts at: 1087 1447 1087 Size: 360 3302 RsrII CG'GwC_CG Cuts at: 1857 1857 Size: 3662 SacI G_AGCT'C Cuts at: 548 676 548 Size: 128 3534 SalI G'TCGA_C Cuts at: 699 699 Size: 3662 SapI GCTCTTCn'nnn_ Cuts at: 1685 1895 1685 Size: 210 3452 SbfI CC_TGCA'GG Cuts at: 147 709 147 Size: 562 3100 ScaI AGT'ACT Cuts at: 3179 3179 Size: 3662 SfcI C'TryA_G Cuts at: 33 143 219 477 705 1390 2383 2574 33 Size: 110 76 258 228 685 993 191 1121 Fragments arranged by size: 1121 993 685 258 228 191 110 76 SfoI GGC'GCC Cuts at: 1094 1342 1094 Size: 248 3414 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3662 SmaI CCC'GGG Cuts at: 684 684 Size: 3662 SmlI C'TyrA_G Cuts at: 817 1732 2224 2486 817 Size: 915 492 262 1993 Fragments arranged by size: 1993 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3662 SphI G_CATG'C Cuts at: 715 1215 715 Size: 500 3162 StuI AGG'CCT Cuts at: 444 444 Size: 3662 StyI C'CwwG_G Cuts at: 796 1150 796 Size: 354 3308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 229 1245 1378 1837 2046 229 Size: 1016 133 459 209 1845 Fragments arranged by size: 1845 1016 459 209 133 TatI w'GTAC_w Cuts at: 209 3177 209 Size: 2968 694 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3179 3211 3179 Size: 32 3630 Tth111I GACn'n_nGTC Cuts at: 202 1459 202 Size: 1257 2405 XbaI T'CTAG_A Cuts at: 693 693 Size: 3662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1157 1157 Size: 3662 XmaI C'CCGG_G Cuts at: 682 682 Size: 3662 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI