Restriction map for
pF1KB6890
(Circular) (Six-base) MAPSORT of: pF1KB6890.seq Check: 4072 from: 1 to: 3719
pF1KB6890 3719 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 409 409
Size: 3719
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AflIII A'CryG_T
Cuts at: 317 1071 2175 3152 317
Size: 754 1104 977 884
Fragments arranged by size:
1104 977 884 754
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 367 1024 2591 367
Size: 657 1567 1495
Fragments arranged by size:
1567 1495 657
ApaI G_GGCC'C
Cuts at: 298 298
Size: 3719
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 262 723 3072 3328 262
Size: 461 2349 256 653
Fragments arranged by size:
2349 653 461 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 150 539 739 150
Size: 389 200 3130
Fragments arranged by size:
3130 389 200
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 157 298 665 733 1763 157
Size: 141 367 68 1030 2113
Fragments arranged by size:
2113 1030 367 141 68
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 267 1239 267
Size: 972 2747
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 298 1344 1437 2493 298
Size: 1046 93 1056 1524
Fragments arranged by size:
1524 1056 1046 93
BmrI ACTGGGnnnn_n'
Cuts at: 573 931 1012 1347 3491 573
Size: 358 81 335 2144 801
Fragments arranged by size:
2144 801 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 241 496 607 163
Size: 78 255 111 3275
Fragments arranged by size:
3275 255 111 78
Bpu10I CC'TnA_GC
Cuts at: 467 467
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 592 3423 592
Size: 2831 888
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 338 1150 1398 2100 3596 338
Size: 812 248 702 1496 461
Fragments arranged by size:
1496 812 702 461 248
BsaWI w'CCGG_w
Cuts at: 173 908 1429 2107 2381 2528 2939 173
Size: 735 521 678 274 147 411 953
Fragments arranged by size:
953 735 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 676
Size: 3719
BseYI C'CCAG_C
Cuts at: 506 1144 1902 2479 2975 506
Size: 638 758 577 496 1250
Fragments arranged by size:
1250 758 638 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 216 387 624 216
Size: 171 237 3311
Fragments arranged by size:
3311 237 171
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 157 298 665 733 1344 1437 1511 1701 1763
Size: 141 367 68 611 93 74 190 62
Cuts at: 1763 2493 157
Size: 730 1383
Fragments arranged by size:
1383 730 611 367 190 141 93 74
68 62
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 409 769 983 1285 1666 409
Size: 360 214 302 381 2462
Fragments arranged by size:
2462 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 1717 1898 2107 1717
Size: 181 209 3329
Fragments arranged by size:
3329 209 181
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstEII G'GTnAC_C
Cuts at: 543 543
Size: 3719
BstYI r'GATC_y
Cuts at: 1 272 744 1211 1234 1569 1815 2827 2913
Size: 271 472 467 23 335 246 1012 86
Cuts at: 2913 2925 3271 1
Size: 12 346 449
Fragments arranged by size:
1012 472 467 449 346 335 271 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 374 411 701 107
Size: 267 37 290 3125
Fragments arranged by size:
3125 290 267 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 204 962 204
Size: 758 2961
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 110 1304 1478 1869 1896 3241 110
Size: 1194 174 391 27 1345 588
Fragments arranged by size:
1345 1194 588 391 174 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 226 1927 2237 2383 3529 226
Size: 1701 310 146 1146 416
Fragments arranged by size:
1701 1146 416 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 241 496 607 1544 1976 2723 2991 163
Size: 78 255 111 937 432 747 268 891
Fragments arranged by size:
937 891 747 432 268 255 111 78
EcoO109I rG'GnC_Cy
Cuts at: 295 606 858 295
Size: 311 252 3156
Fragments arranged by size:
3156 311 252
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1561 1591 1593 1623 1561
Size: 30 2 30 3657
Fragments arranged by size:
3657 30 30 2
HincII GTy'rAC
Cuts at: 447 758 447
Size: 311 3408
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 112 1480 112
Size: 1368 2351
MslI CAynn'nnrTG
Cuts at: 430 1708 1835 430
Size: 1278 127 2314
Fragments arranged by size:
2314 1278 127
MspA1I CmG'CkG
Cuts at: 516 830 1144 1504 2517 2762 516
Size: 314 314 360 1013 245 1473
Fragments arranged by size:
1473 1013 360 314 314 245
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NcoI C'CATG_G
Cuts at: 107 411 701 107
Size: 304 290 3125
Fragments arranged by size:
3125 304 290
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NspI r_CATG'y
Cuts at: 321 772 1272 2179 321
Size: 451 500 907 1861
Fragments arranged by size:
1861 907 500 451
PasI CC'CwG_GG
Cuts at: 615 615
Size: 3719
PciI A'CATG_T
Cuts at: 317 2175 317
Size: 1858 1861
PfoI T'CCnGG_A
Cuts at: 140 140
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PpuMI rG'GwC_Cy
Cuts at: 606 606
Size: 3719
PshAI GACnn'nnGTC
Cuts at: 388 388
Size: 3719
PspOMI G'GGCC_C
Cuts at: 294 294
Size: 3719
PstI C_TGCA'G
Cuts at: 766 1451 766
Size: 685 3034
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 762 1447 2440 2631 33
Size: 729 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 541 741 541
Size: 200 3519
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 107 113 411 701 853 1207 107
Size: 6 298 290 152 354 2619
Fragments arranged by size:
2619 354 298 290 152 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 367 1302 1435 1894 2103 367
Size: 935 133 459 209 1983
Fragments arranged by size:
1983 935 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1214 1214
Size: 3719
XmaI C'CCGG_G
Cuts at: 539 739 539
Size: 200 3519
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI