Restriction map for
pF1KB6891
(Circular) (Six-base) MAPSORT of: pF1KB6891.seq Check: 7743 from: 1 to: 3662
pF1KB6891 3662 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 120 522 120
Size: 402 3260
AccI GT'mk_AC
Cuts at: 700 700
Size: 3662
Acc65I G'GTAC_C
Cuts at: 678 678
Size: 3662
AclI AA'CG_TT
Cuts at: 3502 3502
Size: 3662
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1487 1919 2666 2934 340
Size: 1147 432 747 268 1068
Fragments arranged by size:
1147 1068 747 432 268
AflIII A'CryG_T
Cuts at: 1014 2118 3095 1014
Size: 1104 977 1581
Fragments arranged by size:
1581 1104 977
AgeI A'CCGG_T
Cuts at: 2050 2050
Size: 3662
AlwNI CAG_nnn'CTG
Cuts at: 967 2534 967
Size: 1567 2095
ApaLI G'TGCA_C
Cuts at: 2432 2432
Size: 3662
ApoI r'AATT_y
Cuts at: 666 3015 3271 666
Size: 2349 256 1057
Fragments arranged by size:
2349 1057 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3662
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
AvaI C'yCGr_G
Cuts at: 682 682
Size: 3662
BamHI G'GATC_C
Cuts at: 687 687
Size: 3662
BanI G'GyrC_C
Cuts at: 678 1092 1340 1375 678
Size: 414 248 35 2965
Fragments arranged by size:
2965 414 248 35
BanII G_rGCy'C
Cuts at: 194 676 1706 194
Size: 482 1030 2150
Fragments arranged by size:
2150 1030 482
BbeI G_GCGC'C
Cuts at: 1096 1344 1096
Size: 248 3414
BciVI GTATCCnnnnn_n'
Cuts at: 1560 2321 3662 1560
Size: 761 1341 1560
Fragments arranged by size:
1560 1341 761
BclI T'GATC_A
Cuts at: 1182 1182
Size: 3662
BglII A'GATC_T
Cuts at: 1177 3214 1177
Size: 2037 1625
BlpI GC'TnA_GC
Cuts at: 774 774
Size: 3662
Bme1580I G_kGCm'C
Cuts at: 1287 1380 2436 1287
Size: 93 1056 2513
Fragments arranged by size:
2513 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 396 874 955 1290 3434 396
Size: 478 81 335 2144 624
Fragments arranged by size:
2144 624 478 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 3662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 3662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1717 2209 2507 838
Size: 879 492 298 1993
Fragments arranged by size:
1993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3366 3366
Size: 3662
BsaAI yAC'GTr
Cuts at: 564 1015 564
Size: 451 3211
BsaBI GATnn'nnATC
Cuts at: 6 1176 3213 6
Size: 1170 2037 455
Fragments arranged by size:
2037 1170 455
BsaHI Gr'CG_yC
Cuts at: 1093 1341 2043 3539 1093
Size: 248 702 1496 1216
Fragments arranged by size:
1496 1216 702 248
BsaWI w'CCGG_w
Cuts at: 851 1372 2050 2324 2471 2882 851
Size: 521 678 274 147 411 1631
Fragments arranged by size:
1631 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 307
Size: 3662
BseYI C'CCAG_C
Cuts at: 1087 1845 2422 2918 1087
Size: 758 577 496 1831
Fragments arranged by size:
1831 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 3662
BsiEI CG_ry'CG
Cuts at: 104 116 1204 1250 2458 104
Size: 12 1088 46 1208 1308
Fragments arranged by size:
1308 1208 1088 46 12
BsiHKAI G_wGCw'C
Cuts at: 194 676 1454 1644 2436 194
Size: 482 778 190 792 1420
Fragments arranged by size:
1420 792 778 482 190
BsmI GAATG_Cn'
Cuts at: 408 408
Size: 3662
Bsp1286I G_dGCh'C
Cuts at: 194 676 1287 1380 1454 1644 1706 2436 194
Size: 482 611 93 74 190 62 730 1420
Fragments arranged by size:
1420 730 611 482 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2882 2882
Size: 3662
BspHI T'CATG_A
Cuts at: 288 2838 288
Size: 2550 1112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 522 712 926 1228 1609 120
Size: 402 190 214 302 381 2173
Fragments arranged by size:
2173 402 381 302 214 190
BsrBI CCG'CTC
Cuts at: 50 395 1954 2008 3492 3657 50
Size: 345 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 345 165 55 54
BsrDI GCAATG_nn'
Cuts at: 413 1574 413
Size: 1161 2501
BsrFI r'CCGG_y
Cuts at: 1660 1841 2050 1660
Size: 181 209 3272
Fragments arranged by size:
3272 209 181
BssSI C'ACGA_G
Cuts at: 1933 2291 1933
Size: 358 3304
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1039
Size: 3662
BstBI TT'CG_AA
Cuts at: 2023 2023
Size: 3662
BstEII G'GTnAC_C
Cuts at: 556 556
Size: 3662
BstXI CCAn_nnnn'nTGG
Cuts at: 340 340
Size: 3662
BstYI r'GATC_y
Cuts at: 1 687 1154 1177 1512 1758 2770 2856 2868
Size: 686 467 23 335 246 1012 86 12
Cuts at: 2868 3214 1
Size: 346 449
Fragments arranged by size:
1012 686 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 254 1612 1793 1956 254
Size: 1358 181 163 1960
Fragments arranged by size:
1960 1358 181 163
BtsI GCAGTG_nn'
Cuts at: 905 905
Size: 3662
DraI TTT'AAA
Cuts at: 661 661
Size: 3662
DrdI GACnn_nn'nnGTC
Cuts at: 117 1368 2226 117
Size: 1251 858 1553
Fragments arranged by size:
1553 1251 858
EaeI y'GGCC_r
Cuts at: 342 470 1247 1421 1812 1839 3184 342
Size: 128 777 174 391 27 1345 820
Fragments arranged by size:
1345 820 777 391 174 128 27
EagI C'GGCC_G
Cuts at: 1247 1247
Size: 3662
EarI CTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 622 1870 2180 2326 3472 622
Size: 1248 310 146 1146 812
Fragments arranged by size:
1248 1146 812 310 146
EcoICRI GAG'CTC
Cuts at: 192 674 192
Size: 482 3180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 340 1487 1919 2666 2934 208
Size: 132 1147 432 747 268 936
Fragments arranged by size:
1147 936 747 432 268 132
EcoO109I rG'GnC_Cy
Cuts at: 801 801
Size: 3662
EcoRI G'AATT_C
Cuts at: 666 666
Size: 3662
EcoRV GAT'ATC
Cuts at: 363 363
Size: 3662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1681 1681
Size: 3662
FspI TGC'GCA
Cuts at: 577 1443 577
Size: 866 2796
HaeII r_GCGC'y
Cuts at: 592 1096 1344 2366 3345 592
Size: 504 248 1022 979 909
Fragments arranged by size:
1022 979 909 504 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1504 1534 1536 1566 1504
Size: 30 2 30 3600
Fragments arranged by size:
3600 30 30 2
HincII GTy'rAC
Cuts at: 701 701
Size: 3662
KasI G'GCGC_C
Cuts at: 1092 1340 1092
Size: 248 3414
KpnI G_GTAC'C
Cuts at: 682 682
Size: 3662
MluI A'CGCG_T
Cuts at: 3095 3095
Size: 3662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2517 2333
Size: 184 3478
MscI TGG'CCA
Cuts at: 472 1423 472
Size: 951 2711
MslI CAynn'nnrTG
Cuts at: 497 1651 1778 497
Size: 1154 127 2381
Fragments arranged by size:
2381 1154 127
MspA1I CmG'CkG
Cuts at: 773 1087 1447 2460 2705 773
Size: 314 360 1013 245 1730
Fragments arranged by size:
1730 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1843 1843
Size: 3662
NarI GG'CG_CC
Cuts at: 1093 1341 1093
Size: 248 3414
NgoMIV G'CCGG_C
Cuts at: 1841 1841
Size: 3662
NspI r_CATG'y
Cuts at: 307 715 1215 2122 307
Size: 408 500 907 1847
Fragments arranged by size:
1847 907 500 408
PasI CC'CwG_GG
Cuts at: 633 633
Size: 3662
PciI A'CATG_T
Cuts at: 2118 2118
Size: 3662
PmeI GTTT'AAAC
Cuts at: 661 661
Size: 3662
PmlI CAC'GTG
Cuts at: 564 564
Size: 3662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 3630
PstI C_TGCA'G
Cuts at: 329 709 1394 329
Size: 380 685 2597
Fragments arranged by size:
2597 685 380
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3662
PvuII CAG'CTG
Cuts at: 1087 1447 1087
Size: 360 3302
RsrII CG'GwC_CG
Cuts at: 1857 1857
Size: 3662
SacI G_AGCT'C
Cuts at: 194 676 194
Size: 482 3180
SalI G'TCGA_C
Cuts at: 699 699
Size: 3662
SapI GCTCTTCn'nnn_
Cuts at: 1685 1895 1685
Size: 210 3452
SbfI CC_TGCA'GG
Cuts at: 709 709
Size: 3662
ScaI AGT'ACT
Cuts at: 3179 3179
Size: 3662
SfcI C'TryA_G
Cuts at: 33 325 705 1390 2383 2574 33
Size: 292 380 685 993 191 1121
Fragments arranged by size:
1121 993 685 380 292 191
SfoI GGC'GCC
Cuts at: 1094 1342 1094
Size: 248 3414
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3662
SmaI CCC'GGG
Cuts at: 684 684
Size: 3662
SmlI C'TyrA_G
Cuts at: 817 1732 2224 2486 817
Size: 915 492 262 1993
Fragments arranged by size:
1993 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3662
SphI G_CATG'C
Cuts at: 715 1215 715
Size: 500 3162
StyI C'CwwG_G
Cuts at: 796 1150 796
Size: 354 3308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 544 1245 1378 1837 2046 544
Size: 701 133 459 209 2160
Fragments arranged by size:
2160 701 459 209 133
TatI w'GTAC_w
Cuts at: 3177 3177
Size: 3662
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 3630
Tth111I GACn'n_nGTC
Cuts at: 117 1459 117
Size: 1342 2320
XbaI T'CTAG_A
Cuts at: 693 693
Size: 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1157 549
Size: 608 3054
XmaI C'CCGG_G
Cuts at: 682 682
Size: 3662
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI