(Circular) (Six-base) MAPSORT of: pF1KB6893.seq Check: 2874 from: 1 to: 3665 pF1KB6893 3665 bp With 182 enzymes: SgfI * April 24, 2009 13:06 .. AccI GT'mk_AC Cuts at: 521 703 521 Size: 182 3483 Acc65I G'GTAC_C Cuts at: 681 681 Size: 3665 AclI AA'CG_TT Cuts at: 3505 3505 Size: 3665 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1490 1922 2669 2937 1490 Size: 432 747 268 2218 Fragments arranged by size: 2218 747 432 268 AflIII A'CryG_T Cuts at: 214 1017 2121 3098 214 Size: 803 1104 977 781 Fragments arranged by size: 1104 977 803 781 AgeI A'CCGG_T Cuts at: 2053 2053 Size: 3665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 625 625 Size: 3665 AlwNI CAG_nnn'CTG Cuts at: 970 2537 970 Size: 1567 2098 ApaLI G'TGCA_C Cuts at: 2435 2435 Size: 3665 ApoI r'AATT_y Cuts at: 669 3018 3274 669 Size: 2349 256 1060 Fragments arranged by size: 2349 1060 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3665 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3665 AvaI C'yCGr_G Cuts at: 463 685 463 Size: 222 3443 BamHI G'GATC_C Cuts at: 690 690 Size: 3665 BanI G'GyrC_C Cuts at: 681 1095 1343 1378 681 Size: 414 248 35 2968 Fragments arranged by size: 2968 414 248 35 BanII G_rGCy'C Cuts at: 679 1709 679 Size: 1030 2635 BbeI G_GCGC'C Cuts at: 1099 1347 1099 Size: 248 3417 BbsI GAAGACnn'nnnn_ Cuts at: 500 500 Size: 3665 BciVI GTATCCnnnnn_n' Cuts at: 1563 2324 3665 1563 Size: 761 1341 1563 Fragments arranged by size: 1563 1341 761 BclI T'GATC_A Cuts at: 1185 1185 Size: 3665 BglII A'GATC_T Cuts at: 1180 3217 1180 Size: 2037 1628 BlpI GC'TnA_GC Cuts at: 777 777 Size: 3665 Bme1580I G_kGCm'C Cuts at: 1290 1383 2439 1290 Size: 93 1056 2516 Fragments arranged by size: 2516 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 877 958 1293 3437 877 Size: 81 335 2144 1105 Fragments arranged by size: 2144 1105 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 3665 Bpu10I CC'TnA_GC Cuts at: 337 337 Size: 3665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 841 1720 2212 2510 841 Size: 879 492 298 1996 Fragments arranged by size: 1996 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 397 3369 397 Size: 2972 693 BsaAI yAC'GTr Cuts at: 1018 1018 Size: 3665 BsaBI GATnn'nnATC Cuts at: 6 1179 3216 6 Size: 1173 2037 455 Fragments arranged by size: 2037 1173 455 BsaHI Gr'CG_yC Cuts at: 1096 1344 2046 3542 1096 Size: 248 702 1496 1219 Fragments arranged by size: 1496 1219 702 248 BsaWI w'CCGG_w Cuts at: 854 1375 2053 2327 2474 2885 854 Size: 521 678 274 147 411 1634 Fragments arranged by size: 1634 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 194 197 194 Size: 3 3662 BseYI C'CCAG_C Cuts at: 121 546 1090 1848 2425 2921 121 Size: 425 544 758 577 496 865 Fragments arranged by size: 865 758 577 544 496 425 BsiEI CG_ry'CG Cuts at: 104 1207 1253 2461 104 Size: 1103 46 1208 1308 Fragments arranged by size: 1308 1208 1103 46 BsiHKAI G_wGCw'C Cuts at: 133 679 1457 1647 2439 133 Size: 546 778 190 792 1359 Fragments arranged by size: 1359 792 778 546 190 BsmI GAATG_Cn' Cuts at: 270 487 270 Size: 217 3448 Bsp1286I G_dGCh'C Cuts at: 133 679 1290 1383 1457 1647 1709 2439 133 Size: 546 611 93 74 190 62 730 1359 Fragments arranged by size: 1359 730 611 546 190 93 74 62 BspEI T'CCGG_A Cuts at: 2885 2885 Size: 3665 BspHI T'CATG_A Cuts at: 2841 2841 Size: 3665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 715 929 1231 1612 715 Size: 214 302 381 2768 Fragments arranged by size: 2768 381 302 214 BsrBI CCG'CTC Cuts at: 50 1957 2011 3495 3660 50 Size: 1907 54 1484 165 55 Fragments arranged by size: 1907 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 531 1577 531 Size: 1046 2619 BsrFI r'CCGG_y Cuts at: 1663 1844 2053 1663 Size: 181 209 3275 Fragments arranged by size: 3275 209 181 BssSI C'ACGA_G Cuts at: 1936 2294 1936 Size: 358 3307 BstAPI GCAn_nnn'nTGC Cuts at: 1042 1042 Size: 3665 BstBI TT'CG_AA Cuts at: 2026 2026 Size: 3665 BstYI r'GATC_y Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871 Size: 689 467 23 335 246 1012 86 12 Cuts at: 2871 3217 1 Size: 346 449 Fragments arranged by size: 1012 689 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1615 1796 1959 1615 Size: 181 163 3321 Fragments arranged by size: 3321 181 163 BtsI GCAGTG_nn' Cuts at: 133 908 133 Size: 775 2890 DraI TTT'AAA Cuts at: 375 664 375 Size: 289 3376 DrdI GACnn_nn'nnGTC Cuts at: 1371 2229 1371 Size: 858 2807 EaeI y'GGCC_r Cuts at: 1250 1424 1815 1842 3187 1250 Size: 174 391 27 1345 1728 Fragments arranged by size: 1728 1345 391 174 27 EagI C'GGCC_G Cuts at: 1250 1250 Size: 3665 EarI CTCTTCn'nnn_ Cuts at: 1688 1898 1688 Size: 210 3455 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1873 2183 2329 3475 1873 Size: 310 146 1146 2063 Fragments arranged by size: 2063 1146 310 146 EcoICRI GAG'CTC Cuts at: 677 677 Size: 3665 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1490 1922 2669 2937 1490 Size: 432 747 268 2218 Fragments arranged by size: 2218 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 804 804 Size: 3665 EcoRI G'AATT_C Cuts at: 669 669 Size: 3665 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1684 1684 Size: 3665 FspI TGC'GCA Cuts at: 1446 1446 Size: 3665 HaeII r_GCGC'y Cuts at: 1099 1347 2369 3348 1099 Size: 248 1022 979 1416 Fragments arranged by size: 1416 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 377 409 1507 1537 1539 1569 377 Size: 32 1098 30 2 30 2473 Fragments arranged by size: 2473 1098 32 30 30 2 HincII GTy'rAC Cuts at: 181 522 704 181 Size: 341 182 3142 Fragments arranged by size: 3142 341 182 KasI G'GCGC_C Cuts at: 1095 1343 1095 Size: 248 3417 KpnI G_GTAC'C Cuts at: 685 685 Size: 3665 MluI A'CGCG_T Cuts at: 3098 3098 Size: 3665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2336 2520 2336 Size: 184 3481 MscI TGG'CCA Cuts at: 1426 1426 Size: 3665 MslI CAynn'nnrTG Cuts at: 1654 1781 1654 Size: 127 3538 MspA1I CmG'CkG Cuts at: 616 776 1090 1450 2463 2708 616 Size: 160 314 360 1013 245 1573 Fragments arranged by size: 1573 1013 360 314 245 160 NaeI GCC'GGC Cuts at: 1846 1846 Size: 3665 NarI GG'CG_CC Cuts at: 1096 1344 1096 Size: 248 3417 NgoMIV G'CCGG_C Cuts at: 1844 1844 Size: 3665 NspI r_CATG'y Cuts at: 166 218 718 1218 2125 166 Size: 52 500 500 907 1706 Fragments arranged by size: 1706 907 500 500 52 PciI A'CATG_T Cuts at: 214 2121 214 Size: 1907 1758 PmeI GTTT'AAAC Cuts at: 664 664 Size: 3665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2804 2836 2804 Size: 32 3633 PspXI vC'TCGA_Gb Cuts at: 463 463 Size: 3665 PstI C_TGCA'G Cuts at: 712 1397 712 Size: 685 2980 PvuI CG_AT'CG Cuts at: 104 104 Size: 3665 PvuII CAG'CTG Cuts at: 616 1090 1450 616 Size: 474 360 2831 Fragments arranged by size: 2831 474 360 RsrII CG'GwC_CG Cuts at: 1860 1860 Size: 3665 SacI G_AGCT'C Cuts at: 679 679 Size: 3665 SalI G'TCGA_C Cuts at: 520 702 520 Size: 182 3483 SapI GCTCTTCn'nnn_ Cuts at: 1688 1898 1688 Size: 210 3455 SbfI CC_TGCA'GG Cuts at: 712 712 Size: 3665 ScaI AGT'ACT Cuts at: 3182 3182 Size: 3665 SfcI C'TryA_G Cuts at: 33 708 1393 2386 2577 33 Size: 675 685 993 191 1121 Fragments arranged by size: 1121 993 685 675 191 SfoI GGC'GCC Cuts at: 1097 1345 1097 Size: 248 3417 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3665 SmaI CCC'GGG Cuts at: 687 687 Size: 3665 SmlI C'TyrA_G Cuts at: 463 820 1735 2227 2489 463 Size: 357 915 492 262 1639 Fragments arranged by size: 1639 915 492 357 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3665 SphI G_CATG'C Cuts at: 166 718 1218 166 Size: 552 500 2613 Fragments arranged by size: 2613 552 500 SspI AAT'ATT Cuts at: 145 145 Size: 3665 StuI AGG'CCT Cuts at: 461 461 Size: 3665 StyI C'CwwG_G Cuts at: 799 1153 799 Size: 354 3311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1248 1381 1840 2049 1248 Size: 133 459 209 2864 Fragments arranged by size: 2864 459 209 133 TatI w'GTAC_w Cuts at: 467 3180 467 Size: 2713 952 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3182 3214 3182 Size: 32 3633 Tth111I GACn'n_nGTC Cuts at: 1462 1462 Size: 3665 XbaI T'CTAG_A Cuts at: 696 696 Size: 3665 XcmI CCAnnnn_n'nnnnTGG Cuts at: 114 1160 114 Size: 1046 2619 XhoI C'TCGA_G Cuts at: 463 463 Size: 3665 XmaI C'CCGG_G Cuts at: 685 685 Size: 3665 XmnI GAAnn'nnTTC Cuts at: 446 446 Size: 3665 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI