Restriction map for
pF1KB6893
(Circular) (Six-base) MAPSORT of: pF1KB6893.seq Check: 2874 from: 1 to: 3665
pF1KB6893 3665 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 521 703 521
Size: 182 3483
Acc65I G'GTAC_C
Cuts at: 681 681
Size: 3665
AclI AA'CG_TT
Cuts at: 3505 3505
Size: 3665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2669 2937 1490
Size: 432 747 268 2218
Fragments arranged by size:
2218 747 432 268
AflIII A'CryG_T
Cuts at: 214 1017 2121 3098 214
Size: 803 1104 977 781
Fragments arranged by size:
1104 977 803 781
AgeI A'CCGG_T
Cuts at: 2053 2053
Size: 3665
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 3665
AlwNI CAG_nnn'CTG
Cuts at: 970 2537 970
Size: 1567 2098
ApaLI G'TGCA_C
Cuts at: 2435 2435
Size: 3665
ApoI r'AATT_y
Cuts at: 669 3018 3274 669
Size: 2349 256 1060
Fragments arranged by size:
2349 1060 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3665
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
AvaI C'yCGr_G
Cuts at: 463 685 463
Size: 222 3443
BamHI G'GATC_C
Cuts at: 690 690
Size: 3665
BanI G'GyrC_C
Cuts at: 681 1095 1343 1378 681
Size: 414 248 35 2968
Fragments arranged by size:
2968 414 248 35
BanII G_rGCy'C
Cuts at: 679 1709 679
Size: 1030 2635
BbeI G_GCGC'C
Cuts at: 1099 1347 1099
Size: 248 3417
BbsI GAAGACnn'nnnn_
Cuts at: 500 500
Size: 3665
BciVI GTATCCnnnnn_n'
Cuts at: 1563 2324 3665 1563
Size: 761 1341 1563
Fragments arranged by size:
1563 1341 761
BclI T'GATC_A
Cuts at: 1185 1185
Size: 3665
BglII A'GATC_T
Cuts at: 1180 3217 1180
Size: 2037 1628
BlpI GC'TnA_GC
Cuts at: 777 777
Size: 3665
Bme1580I G_kGCm'C
Cuts at: 1290 1383 2439 1290
Size: 93 1056 2516
Fragments arranged by size:
2516 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 877 958 1293 3437 877
Size: 81 335 2144 1105
Fragments arranged by size:
2144 1105 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 3665
Bpu10I CC'TnA_GC
Cuts at: 337 337
Size: 3665
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1720 2212 2510 841
Size: 879 492 298 1996
Fragments arranged by size:
1996 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 397 3369 397
Size: 2972 693
BsaAI yAC'GTr
Cuts at: 1018 1018
Size: 3665
BsaBI GATnn'nnATC
Cuts at: 6 1179 3216 6
Size: 1173 2037 455
Fragments arranged by size:
2037 1173 455
BsaHI Gr'CG_yC
Cuts at: 1096 1344 2046 3542 1096
Size: 248 702 1496 1219
Fragments arranged by size:
1496 1219 702 248
BsaWI w'CCGG_w
Cuts at: 854 1375 2053 2327 2474 2885 854
Size: 521 678 274 147 411 1634
Fragments arranged by size:
1634 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 197 194
Size: 3 3662
BseYI C'CCAG_C
Cuts at: 121 546 1090 1848 2425 2921 121
Size: 425 544 758 577 496 865
Fragments arranged by size:
865 758 577 544 496 425
BsiEI CG_ry'CG
Cuts at: 104 1207 1253 2461 104
Size: 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1103 46
BsiHKAI G_wGCw'C
Cuts at: 133 679 1457 1647 2439 133
Size: 546 778 190 792 1359
Fragments arranged by size:
1359 792 778 546 190
BsmI GAATG_Cn'
Cuts at: 270 487 270
Size: 217 3448
Bsp1286I G_dGCh'C
Cuts at: 133 679 1290 1383 1457 1647 1709 2439 133
Size: 546 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 546 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2885 2885
Size: 3665
BspHI T'CATG_A
Cuts at: 2841 2841
Size: 3665
BspMI ACCTGCnnnn'nnnn_
Cuts at: 715 929 1231 1612 715
Size: 214 302 381 2768
Fragments arranged by size:
2768 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1957 2011 3495 3660 50
Size: 1907 54 1484 165 55
Fragments arranged by size:
1907 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 531 1577 531
Size: 1046 2619
BsrFI r'CCGG_y
Cuts at: 1663 1844 2053 1663
Size: 181 209 3275
Fragments arranged by size:
3275 209 181
BssSI C'ACGA_G
Cuts at: 1936 2294 1936
Size: 358 3307
BstAPI GCAn_nnn'nTGC
Cuts at: 1042 1042
Size: 3665
BstBI TT'CG_AA
Cuts at: 2026 2026
Size: 3665
BstYI r'GATC_y
Cuts at: 1 690 1157 1180 1515 1761 2773 2859 2871
Size: 689 467 23 335 246 1012 86 12
Cuts at: 2871 3217 1
Size: 346 449
Fragments arranged by size:
1012 689 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1615 1796 1959 1615
Size: 181 163 3321
Fragments arranged by size:
3321 181 163
BtsI GCAGTG_nn'
Cuts at: 133 908 133
Size: 775 2890
DraI TTT'AAA
Cuts at: 375 664 375
Size: 289 3376
DrdI GACnn_nn'nnGTC
Cuts at: 1371 2229 1371
Size: 858 2807
EaeI y'GGCC_r
Cuts at: 1250 1424 1815 1842 3187 1250
Size: 174 391 27 1345 1728
Fragments arranged by size:
1728 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1250 1250
Size: 3665
EarI CTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1873 2183 2329 3475 1873
Size: 310 146 1146 2063
Fragments arranged by size:
2063 1146 310 146
EcoICRI GAG'CTC
Cuts at: 677 677
Size: 3665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1490 1922 2669 2937 1490
Size: 432 747 268 2218
Fragments arranged by size:
2218 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 804 804
Size: 3665
EcoRI G'AATT_C
Cuts at: 669 669
Size: 3665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1684 1684
Size: 3665
FspI TGC'GCA
Cuts at: 1446 1446
Size: 3665
HaeII r_GCGC'y
Cuts at: 1099 1347 2369 3348 1099
Size: 248 1022 979 1416
Fragments arranged by size:
1416 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 1507 1537 1539 1569 377
Size: 32 1098 30 2 30 2473
Fragments arranged by size:
2473 1098 32 30 30 2
HincII GTy'rAC
Cuts at: 181 522 704 181
Size: 341 182 3142
Fragments arranged by size:
3142 341 182
KasI G'GCGC_C
Cuts at: 1095 1343 1095
Size: 248 3417
KpnI G_GTAC'C
Cuts at: 685 685
Size: 3665
MluI A'CGCG_T
Cuts at: 3098 3098
Size: 3665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2520 2336
Size: 184 3481
MscI TGG'CCA
Cuts at: 1426 1426
Size: 3665
MslI CAynn'nnrTG
Cuts at: 1654 1781 1654
Size: 127 3538
MspA1I CmG'CkG
Cuts at: 616 776 1090 1450 2463 2708 616
Size: 160 314 360 1013 245 1573
Fragments arranged by size:
1573 1013 360 314 245 160
NaeI GCC'GGC
Cuts at: 1846 1846
Size: 3665
NarI GG'CG_CC
Cuts at: 1096 1344 1096
Size: 248 3417
NgoMIV G'CCGG_C
Cuts at: 1844 1844
Size: 3665
NspI r_CATG'y
Cuts at: 166 218 718 1218 2125 166
Size: 52 500 500 907 1706
Fragments arranged by size:
1706 907 500 500 52
PciI A'CATG_T
Cuts at: 214 2121 214
Size: 1907 1758
PmeI GTTT'AAAC
Cuts at: 664 664
Size: 3665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 3633
PspXI vC'TCGA_Gb
Cuts at: 463 463
Size: 3665
PstI C_TGCA'G
Cuts at: 712 1397 712
Size: 685 2980
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3665
PvuII CAG'CTG
Cuts at: 616 1090 1450 616
Size: 474 360 2831
Fragments arranged by size:
2831 474 360
RsrII CG'GwC_CG
Cuts at: 1860 1860
Size: 3665
SacI G_AGCT'C
Cuts at: 679 679
Size: 3665
SalI G'TCGA_C
Cuts at: 520 702 520
Size: 182 3483
SapI GCTCTTCn'nnn_
Cuts at: 1688 1898 1688
Size: 210 3455
SbfI CC_TGCA'GG
Cuts at: 712 712
Size: 3665
ScaI AGT'ACT
Cuts at: 3182 3182
Size: 3665
SfcI C'TryA_G
Cuts at: 33 708 1393 2386 2577 33
Size: 675 685 993 191 1121
Fragments arranged by size:
1121 993 685 675 191
SfoI GGC'GCC
Cuts at: 1097 1345 1097
Size: 248 3417
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3665
SmaI CCC'GGG
Cuts at: 687 687
Size: 3665
SmlI C'TyrA_G
Cuts at: 463 820 1735 2227 2489 463
Size: 357 915 492 262 1639
Fragments arranged by size:
1639 915 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3665
SphI G_CATG'C
Cuts at: 166 718 1218 166
Size: 552 500 2613
Fragments arranged by size:
2613 552 500
SspI AAT'ATT
Cuts at: 145 145
Size: 3665
StuI AGG'CCT
Cuts at: 461 461
Size: 3665
StyI C'CwwG_G
Cuts at: 799 1153 799
Size: 354 3311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1248 1381 1840 2049 1248
Size: 133 459 209 2864
Fragments arranged by size:
2864 459 209 133
TatI w'GTAC_w
Cuts at: 467 3180 467
Size: 2713 952
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 3633
Tth111I GACn'n_nGTC
Cuts at: 1462 1462
Size: 3665
XbaI T'CTAG_A
Cuts at: 696 696
Size: 3665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1160 114
Size: 1046 2619
XhoI C'TCGA_G
Cuts at: 463 463
Size: 3665
XmaI C'CCGG_G
Cuts at: 685 685
Size: 3665
XmnI GAAnn'nnTTC
Cuts at: 446 446
Size: 3665
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI ZraI