Restriction map for pF1KB6893
 (Circular) (Six-base) MAPSORT of: pF1KB6893.seq  Check: 2874  from: 1  to: 3665



pF1KB6893  3665 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    521     703     521 
   Size:        182    3483
 
Acc65I G'GTAC_C

Cuts at:    681     681 
   Size:       3665
 
AclI AA'CG_TT

Cuts at:   3505    3505 
   Size:       3665
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1490    1922    2669    2937    1490 
   Size:        432     747     268    2218

  Fragments arranged by size: 

                2218     747     432     268
 
AflIII A'CryG_T

Cuts at:    214    1017    2121    3098     214 
   Size:        803    1104     977     781

  Fragments arranged by size: 

                1104     977     803     781
 
AgeI A'CCGG_T

Cuts at:   2053    2053 
   Size:       3665
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    625     625 
   Size:       3665
 
AlwNI CAG_nnn'CTG

Cuts at:    970    2537     970 
   Size:       1567    2098
 
ApaLI G'TGCA_C

Cuts at:   2435    2435 
   Size:       3665
 
ApoI r'AATT_y

Cuts at:    669    3018    3274     669 
   Size:       2349     256    1060

  Fragments arranged by size: 

                2349    1060     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3665
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3665
 
AvaI C'yCGr_G

Cuts at:    463     685     463 
   Size:        222    3443
 
BamHI G'GATC_C

Cuts at:    690     690 
   Size:       3665
 
BanI G'GyrC_C

Cuts at:    681    1095    1343    1378     681 
   Size:        414     248      35    2968

  Fragments arranged by size: 

                2968     414     248      35
 
BanII G_rGCy'C

Cuts at:    679    1709     679 
   Size:       1030    2635
 
BbeI G_GCGC'C

Cuts at:   1099    1347    1099 
   Size:        248    3417
 
BbsI GAAGACnn'nnnn_

Cuts at:    500     500 
   Size:       3665
 
BciVI GTATCCnnnnn_n'

Cuts at:   1563    2324    3665    1563 
   Size:        761    1341    1563

  Fragments arranged by size: 

                1563    1341     761
 
BclI T'GATC_A

Cuts at:   1185    1185 
   Size:       3665
 
BglII A'GATC_T

Cuts at:   1180    3217    1180 
   Size:       2037    1628
 
BlpI GC'TnA_GC

Cuts at:    777     777 
   Size:       3665
 
Bme1580I G_kGCm'C

Cuts at:   1290    1383    2439    1290 
   Size:         93    1056    2516

  Fragments arranged by size: 

                2516    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    877     958    1293    3437     877 
   Size:         81     335    2144    1105

  Fragments arranged by size: 

                2144    1105     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2032    2032 
   Size:       3665
 
Bpu10I CC'TnA_GC

Cuts at:    337     337 
   Size:       3665
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    841    1720    2212    2510     841 
   Size:        879     492     298    1996

  Fragments arranged by size: 

                1996     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    397    3369     397 
   Size:       2972     693
 
BsaAI yAC'GTr

Cuts at:   1018    1018 
   Size:       3665
 
BsaBI GATnn'nnATC

Cuts at:      6    1179    3216       6 
   Size:       1173    2037     455

  Fragments arranged by size: 

                2037    1173     455
 
BsaHI Gr'CG_yC

Cuts at:   1096    1344    2046    3542    1096 
   Size:        248     702    1496    1219

  Fragments arranged by size: 

                1496    1219     702     248
 
BsaWI w'CCGG_w

Cuts at:    854    1375    2053    2327    2474    2885     854 
   Size:        521     678     274     147     411    1634

  Fragments arranged by size: 

                1634     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    194     197     194 
   Size:          3    3662
 
BseYI C'CCAG_C

Cuts at:    121     546    1090    1848    2425    2921     121 
   Size:        425     544     758     577     496     865

  Fragments arranged by size: 

                 865     758     577     544     496     425
 
BsiEI CG_ry'CG

Cuts at:    104    1207    1253    2461     104 
   Size:       1103      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1103      46
 
BsiHKAI G_wGCw'C

Cuts at:    133     679    1457    1647    2439     133 
   Size:        546     778     190     792    1359

  Fragments arranged by size: 

                1359     792     778     546     190
 
BsmI GAATG_Cn'

Cuts at:    270     487     270 
   Size:        217    3448
 
Bsp1286I G_dGCh'C

Cuts at:    133     679    1290    1383    1457    1647    1709    2439     133 
   Size:        546     611      93      74     190      62     730    1359

  Fragments arranged by size: 

                1359     730     611     546     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2885    2885 
   Size:       3665
 
BspHI T'CATG_A

Cuts at:   2841    2841 
   Size:       3665
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    715     929    1231    1612     715 
   Size:        214     302     381    2768

  Fragments arranged by size: 

                2768     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1957    2011    3495    3660      50 
   Size:       1907      54    1484     165      55

  Fragments arranged by size: 

                1907    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    531    1577     531 
   Size:       1046    2619
 
BsrFI r'CCGG_y

Cuts at:   1663    1844    2053    1663 
   Size:        181     209    3275

  Fragments arranged by size: 

                3275     209     181
 
BssSI C'ACGA_G

Cuts at:   1936    2294    1936 
   Size:        358    3307
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1042    1042 
   Size:       3665
 
BstBI TT'CG_AA

Cuts at:   2026    2026 
   Size:       3665
 
BstYI r'GATC_y

Cuts at:      1     690    1157    1180    1515    1761    2773    2859    2871 
   Size:        689     467      23     335     246    1012      86      12

Cuts at:   2871    3217       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     689     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1615    1796    1959    1615 
   Size:        181     163    3321

  Fragments arranged by size: 

                3321     181     163
 
BtsI GCAGTG_nn'

Cuts at:    133     908     133 
   Size:        775    2890
 
DraI TTT'AAA

Cuts at:    375     664     375 
   Size:        289    3376
 
DrdI GACnn_nn'nnGTC

Cuts at:   1371    2229    1371 
   Size:        858    2807
 
EaeI y'GGCC_r

Cuts at:   1250    1424    1815    1842    3187    1250 
   Size:        174     391      27    1345    1728

  Fragments arranged by size: 

                1728    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1250    1250 
   Size:       3665
 
EarI CTCTTCn'nnn_

Cuts at:   1688    1898    1688 
   Size:        210    3455
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1873    2183    2329    3475    1873 
   Size:        310     146    1146    2063

  Fragments arranged by size: 

                2063    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    677     677 
   Size:       3665
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1490    1922    2669    2937    1490 
   Size:        432     747     268    2218

  Fragments arranged by size: 

                2218     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    804     804 
   Size:       3665
 
EcoRI G'AATT_C

Cuts at:    669     669 
   Size:       3665
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1684    1684 
   Size:       3665
 
FspI TGC'GCA

Cuts at:   1446    1446 
   Size:       3665
 
HaeII r_GCGC'y

Cuts at:   1099    1347    2369    3348    1099 
   Size:        248    1022     979    1416

  Fragments arranged by size: 

                1416    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    377     409    1507    1537    1539    1569     377 
   Size:         32    1098      30       2      30    2473

  Fragments arranged by size: 

                2473    1098      32      30      30       2
 
HincII GTy'rAC

Cuts at:    181     522     704     181 
   Size:        341     182    3142

  Fragments arranged by size: 

                3142     341     182
 
KasI G'GCGC_C

Cuts at:   1095    1343    1095 
   Size:        248    3417
 
KpnI G_GTAC'C

Cuts at:    685     685 
   Size:       3665
 
MluI A'CGCG_T

Cuts at:   3098    3098 
   Size:       3665
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2336    2520    2336 
   Size:        184    3481
 
MscI TGG'CCA

Cuts at:   1426    1426 
   Size:       3665
 
MslI CAynn'nnrTG

Cuts at:   1654    1781    1654 
   Size:        127    3538
 
MspA1I CmG'CkG

Cuts at:    616     776    1090    1450    2463    2708     616 
   Size:        160     314     360    1013     245    1573

  Fragments arranged by size: 

                1573    1013     360     314     245     160
 
NaeI GCC'GGC

Cuts at:   1846    1846 
   Size:       3665
 
NarI GG'CG_CC

Cuts at:   1096    1344    1096 
   Size:        248    3417
 
NgoMIV G'CCGG_C

Cuts at:   1844    1844 
   Size:       3665
 
NspI r_CATG'y

Cuts at:    166     218     718    1218    2125     166 
   Size:         52     500     500     907    1706

  Fragments arranged by size: 

                1706     907     500     500      52
 
PciI A'CATG_T

Cuts at:    214    2121     214 
   Size:       1907    1758
 
PmeI GTTT'AAAC

Cuts at:    664     664 
   Size:       3665
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2804    2836    2804 
   Size:         32    3633
 
PspXI vC'TCGA_Gb

Cuts at:    463     463 
   Size:       3665
 
PstI C_TGCA'G

Cuts at:    712    1397     712 
   Size:        685    2980
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3665
 
PvuII CAG'CTG

Cuts at:    616    1090    1450     616 
   Size:        474     360    2831

  Fragments arranged by size: 

                2831     474     360
 
RsrII CG'GwC_CG

Cuts at:   1860    1860 
   Size:       3665
 
SacI G_AGCT'C

Cuts at:    679     679 
   Size:       3665
 
SalI G'TCGA_C

Cuts at:    520     702     520 
   Size:        182    3483
 
SapI GCTCTTCn'nnn_

Cuts at:   1688    1898    1688 
   Size:        210    3455
 
SbfI CC_TGCA'GG

Cuts at:    712     712 
   Size:       3665
 
ScaI AGT'ACT

Cuts at:   3182    3182 
   Size:       3665
 
SfcI C'TryA_G

Cuts at:     33     708    1393    2386    2577      33 
   Size:        675     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     675     191
 
SfoI GGC'GCC

Cuts at:   1097    1345    1097 
   Size:        248    3417
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3665
 
SmaI CCC'GGG

Cuts at:    687     687 
   Size:       3665
 
SmlI C'TyrA_G

Cuts at:    463     820    1735    2227    2489     463 
   Size:        357     915     492     262    1639

  Fragments arranged by size: 

                1639     915     492     357     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3665
 
SphI G_CATG'C

Cuts at:    166     718    1218     166 
   Size:        552     500    2613

  Fragments arranged by size: 

                2613     552     500
 
SspI AAT'ATT

Cuts at:    145     145 
   Size:       3665
 
StuI AGG'CCT

Cuts at:    461     461 
   Size:       3665
 
StyI C'CwwG_G

Cuts at:    799    1153     799 
   Size:        354    3311
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1248    1381    1840    2049    1248 
   Size:        133     459     209    2864

  Fragments arranged by size: 

                2864     459     209     133
 
TatI w'GTAC_w

Cuts at:    467    3180     467 
   Size:       2713     952
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3182    3214    3182 
   Size:         32    3633
 
Tth111I GACn'n_nGTC

Cuts at:   1462    1462 
   Size:       3665
 
XbaI T'CTAG_A

Cuts at:    696     696 
   Size:       3665
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    114    1160     114 
   Size:       1046    2619
 
XhoI C'TCGA_G

Cuts at:    463     463 
   Size:       3665
 
XmaI C'CCGG_G

Cuts at:    685     685 
   Size:       3665
 
XmnI GAAnn'nnTTC

Cuts at:    446     446 
   Size:       3665
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BsaXI     BsgI    BsiWI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI     ZraI
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