Restriction map for
pF1KB6894
(Circular) (Six-base) MAPSORT of: pF1KB6894.seq Check: 6127 from: 1 to: 3719
pF1KB6894 3719 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 1024 2591 1024
Size: 1567 2152
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 467 624 739 467
Size: 157 115 3447
Fragments arranged by size:
3447 157 115
BamHI G'GATC_C
Cuts at: 744 744
Size: 3719
BanI G'GyrC_C
Cuts at: 445 735 1149 1397 1432 445
Size: 290 414 248 35 2732
Fragments arranged by size:
2732 414 290 248 35
BanII G_rGCy'C
Cuts at: 178 733 1763 178
Size: 555 1030 2134
Fragments arranged by size:
2134 1030 555
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BbsI GAAGACnn'nnnn_
Cuts at: 630 630
Size: 3719
BbvCI CC'TCA_GC
Cuts at: 499 499
Size: 3719
BciVI GTATCCnnnnn_n'
Cuts at: 1617 2378 3719 1617
Size: 761 1341 1617
Fragments arranged by size:
1617 1341 761
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglI GCCn_nnn'nGGC
Cuts at: 700 700
Size: 3719
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 137 498 1344 1437 2493 137
Size: 361 846 93 1056 1363
Fragments arranged by size:
1363 1056 846 361 93
BmgBI CAC'GTC
Cuts at: 603 603
Size: 3719
BmrI ACTGGGnnnn_n'
Cuts at: 485 931 1012 1347 3491 485
Size: 446 81 335 2144 713
Fragments arranged by size:
2144 713 446 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
Bpu10I CC'TnA_GC
Cuts at: 499 550 499
Size: 51 3668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3423 3423
Size: 3719
BsaAI yAC'GTr
Cuts at: 588 1072 588
Size: 484 3235
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 1150 1398 2100 3596 1150
Size: 248 702 1496 1273
Fragments arranged by size:
1496 1273 702 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 654 1144 1902 2479 2975 654
Size: 490 758 577 496 1398
Fragments arranged by size:
1398 758 577 496 490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 594 136
Size: 458 3261
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 137 178 498 733 1344 1437 1511 1701 1763
Size: 41 320 235 611 93 74 190 62
Cuts at: 1763 2493 137
Size: 730 1363
Fragments arranged by size:
1363 730 611 320 235 190 93 74
62 41
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 239 2895 239
Size: 2656 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 983 1285 1666 769
Size: 214 302 381 2822
Fragments arranged by size:
2822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2011 2065 3549 3714 50
Size: 1961 54 1484 165 55
Fragments arranged by size:
1961 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 1717 1898 2107 1717
Size: 181 209 3329
Fragments arranged by size:
3329 209 181
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstXI CCAn_nnnn'nTGG
Cuts at: 339 414 339
Size: 75 3644
BstYI r'GATC_y
Cuts at: 1 479 744 1211 1234 1569 1815 2827 2913
Size: 478 265 467 23 335 246 1012 86
Cuts at: 2913 2925 3271 1
Size: 12 346 449
Fragments arranged by size:
1012 478 467 449 346 335 265 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 675 1669 1850 2013 675
Size: 994 181 163 2381
Fragments arranged by size:
2381 994 181 163
BtsI GCAGTG_nn'
Cuts at: 285 962 285
Size: 677 3042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 333 368 333
Size: 35 3684
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 1425 2283 1425
Size: 858 2861
EaeI y'GGCC_r
Cuts at: 1304 1478 1869 1896 3241 1304
Size: 174 391 27 1345 1782
Fragments arranged by size:
1782 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 858 858
Size: 3719
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 654 1738 654
Size: 1084 2635
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1561 1591 1593 1623 1561
Size: 30 2 30 3657
Fragments arranged by size:
3657 30 30 2
HincII GTy'rAC
Cuts at: 198 664 758 198
Size: 466 94 3159
Fragments arranged by size:
3159 466 94
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2390 2574 2390
Size: 184 3535
MscI TGG'CCA
Cuts at: 1480 1480
Size: 3719
MslI CAynn'nnrTG
Cuts at: 433 694 1708 1835 433
Size: 261 1014 127 2317
Fragments arranged by size:
2317 1014 261 127
MspA1I CmG'CkG
Cuts at: 830 1144 1504 2517 2762 830
Size: 314 360 1013 245 1787
Fragments arranged by size:
1787 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PasI CC'CwG_GG
Cuts at: 315 453 315
Size: 138 3581
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PmlI CAC'GTG
Cuts at: 588 588
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2890 2858
Size: 32 3687
PstI C_TGCA'G
Cuts at: 766 1451 766
Size: 685 3034
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SfcI C'TryA_G
Cuts at: 33 762 1447 2440 2631 33
Size: 729 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 469 741 469
Size: 272 3447
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StuI AGG'CCT
Cuts at: 545 709 545
Size: 164 3555
StyI C'CwwG_G
Cuts at: 441 853 1207 441
Size: 412 354 2953
Fragments arranged by size:
2953 412 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 236 1302 1435 1894 2103 236
Size: 1066 133 459 209 1852
Fragments arranged by size:
1852 1066 459 209 133
TatI w'GTAC_w
Cuts at: 3234 3234
Size: 3719
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1214 114
Size: 1100 2619
XmaI C'CCGG_G
Cuts at: 467 739 467
Size: 272 3447
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmtI BpmI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI