Restriction map for
pF1KB6902
(Circular) (Six-base) MAPSORT of: pF1KB6902.seq Check: 5324 from: 1 to: 3782
pF1KB6902 3782 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 372 372
Size: 3782
AatII G_ACGT'C
Cuts at: 176 176
Size: 3782
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 661 661
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 183 624 1087 2654 183
Size: 441 463 1567 1311
Fragments arranged by size:
1567 1311 463 441
ApaI G_GGCC'C
Cuts at: 502 502
Size: 3782
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 786 3135 3391 786
Size: 2349 256 1177
Fragments arranged by size:
2349 1177 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 434 802 434
Size: 368 3414
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 154 798 1212 1460 1495 154
Size: 644 414 248 35 2441
Fragments arranged by size:
2441 644 414 248 35
BanII G_rGCy'C
Cuts at: 502 796 1826 502
Size: 294 1030 2458
Fragments arranged by size:
2458 1030 294
BbeI G_GCGC'C
Cuts at: 158 1216 1464 158
Size: 1058 248 2476
Fragments arranged by size:
2476 1058 248
BbvCI CC'TCA_GC
Cuts at: 446 446
Size: 3782
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 360 1302 360
Size: 942 2840
BglI GCCn_nnn'nGGC
Cuts at: 251 251
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 502 1407 1500 2556 502
Size: 905 93 1056 1728
Fragments arranged by size:
1728 1056 905 93
BmgBI CAC'GTC
Cuts at: 234 306 234
Size: 72 3710
BmrI ACTGGGnnnn_n'
Cuts at: 487 994 1075 1410 3554 487
Size: 507 81 335 2144 715
Fragments arranged by size:
2144 715 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 612 2149 612
Size: 1537 2245
Bpu10I CC'TnA_GC
Cuts at: 446 446
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3486 3486
Size: 3782
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 155 173 1213 1461 2163 3659 155
Size: 18 1040 248 702 1496 278
Fragments arranged by size:
1496 1040 702 278 248 18
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 214 403 132
Size: 82 189 3511
Fragments arranged by size:
3511 189 82
BseYI C'CCAG_C
Cuts at: 546 728 747 1207 1965 2542 3038 546
Size: 182 19 460 758 577 496 1290
Fragments arranged by size:
1290 758 577 496 460 182 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 391 667 382
Size: 9 276 3497
Fragments arranged by size:
3497 276 9
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 796 1574 1764 2556 796
Size: 778 190 792 2022
Fragments arranged by size:
2022 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 502 796 1407 1500 1574 1764 1826 2556 502
Size: 294 611 93 74 190 62 730 1728
Fragments arranged by size:
1728 730 611 294 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 372 832 1046 1348 1729 372
Size: 460 214 302 381 2425
Fragments arranged by size:
2425 460 381 302 214
BsrBI CCG'CTC
Cuts at: 50 134 2074 2128 3612 3777 50
Size: 84 1940 54 1484 165 55
Fragments arranged by size:
1940 1484 165 84 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 151 1780 1961 2170 151
Size: 1629 181 209 1763
Fragments arranged by size:
1763 1629 209 181
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 405 1159 405
Size: 754 3028
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstXI CCAn_nnnn'nTGG
Cuts at: 728 728
Size: 3782
BstYI r'GATC_y
Cuts at: 1 807 1274 1297 1632 1878 2890 2976 2988
Size: 806 467 23 335 246 1012 86 12
Cuts at: 2988 3334 1
Size: 346 449
Fragments arranged by size:
1012 806 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 505 505
Size: 3782
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 540 1732 1913 2076 540
Size: 1192 181 163 2246
Fragments arranged by size:
2246 1192 181 163
BtsI GCAGTG_nn'
Cuts at: 377 622 645 1025 377
Size: 245 23 380 3134
Fragments arranged by size:
3134 380 245 23
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 1488 2346 1488
Size: 858 2924
EaeI y'GGCC_r
Cuts at: 1367 1541 1932 1959 3304 1367
Size: 174 391 27 1345 1845
Fragments arranged by size:
1845 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 393 498 499 921 393
Size: 105 1 422 3254
Fragments arranged by size:
3254 422 105 1
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 158 1216 1464 2486 3465 158
Size: 1058 248 1022 979 475
Fragments arranged by size:
1058 1022 979 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1624 1654 1656 1686 1624
Size: 30 2 30 3720
Fragments arranged by size:
3720 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 154 1212 1460 154
Size: 1058 248 2476
Fragments arranged by size:
2476 1058 248
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 745 2453 2637 137
Size: 608 1708 184 1282
Fragments arranged by size:
1708 1282 608 184
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 259 1771 1898 259
Size: 1512 127 2143
Fragments arranged by size:
2143 1512 127
MspA1I CmG'CkG
Cuts at: 273 405 893 1207 1567 2580 2825 273
Size: 132 488 314 360 1013 245 1230
Fragments arranged by size:
1230 1013 488 360 314 245 132
NaeI GCC'GGC
Cuts at: 153 1963 153
Size: 1810 1972
NarI GG'CG_CC
Cuts at: 155 1213 1461 155
Size: 1058 248 2476
Fragments arranged by size:
2476 1058 248
NdeI CA'TA_TG
Cuts at: 346 346
Size: 3782
NgoMIV G'CCGG_C
Cuts at: 151 1961 151
Size: 1810 1972
NruI TCG'CGA
Cuts at: 416 416
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PfoI T'CCnGG_A
Cuts at: 176 176
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PspOMI G'GGCC_C
Cuts at: 498 498
Size: 3782
PstI C_TGCA'G
Cuts at: 356 829 1514 356
Size: 473 685 2624
Fragments arranged by size:
2624 685 473
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 405 1207 1567 405
Size: 802 360 2620
Fragments arranged by size:
2620 802 360
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 3299 3299
Size: 3782
SfcI C'TryA_G
Cuts at: 33 352 825 1510 2503 2694 33
Size: 319 473 685 993 191 1121
Fragments arranged by size:
1121 993 685 473 319 191
SfoI GGC'GCC
Cuts at: 156 1214 1462 156
Size: 1058 248 2476
Fragments arranged by size:
2476 1058 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 436 804 436
Size: 368 3414
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 916 1270 916
Size: 354 3428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 622 1365 1498 1957 2166 622
Size: 743 133 459 209 2238
Fragments arranged by size:
2238 743 459 209 133
TatI w'GTAC_w
Cuts at: 3297 3297
Size: 3782
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 3750
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 253 1277 253
Size: 1024 2758
XmaI C'CCGG_G
Cuts at: 434 802 434
Size: 368 3414
ZraI GAC'GTC
Cuts at: 174 174
Size: 3782
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NruI NspI PciI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BpmI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI