Restriction map for
pF1KB6906
(Circular) (Six-base) MAPSORT of: pF1KB6906.seq Check: 1700 from: 1 to: 3782
pF1KB6906 3782 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 820 820
Size: 3782
Acc65I G'GTAC_C
Cuts at: 798 798
Size: 3782
AclI AA'CG_TT
Cuts at: 3622 3622
Size: 3782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
AflIII A'CryG_T
Cuts at: 1134 2238 3215 1134
Size: 1104 977 1701
Fragments arranged by size:
1701 1104 977
AgeI A'CCGG_T
Cuts at: 2170 2170
Size: 3782
AleI CACnn'nnGTG
Cuts at: 766 766
Size: 3782
AlwNI CAG_nnn'CTG
Cuts at: 1087 2654 1087
Size: 1567 2215
ApaLI G'TGCA_C
Cuts at: 2552 2552
Size: 3782
ApoI r'AATT_y
Cuts at: 445 474 623 786 3135 3391 445
Size: 29 149 163 2349 256 836
Fragments arranged by size:
2349 836 256 163 149 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 3782
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
AvaI C'yCGr_G
Cuts at: 802 802
Size: 3782
BamHI G'GATC_C
Cuts at: 807 807
Size: 3782
BanI G'GyrC_C
Cuts at: 563 798 1212 1460 1495 563
Size: 235 414 248 35 2850
Fragments arranged by size:
2850 414 248 235 35
BanII G_rGCy'C
Cuts at: 796 1826 796
Size: 1030 2752
BbeI G_GCGC'C
Cuts at: 1216 1464 1216
Size: 248 3534
BbsI GAAGACnn'nnnn_
Cuts at: 414 414
Size: 3782
BciVI GTATCCnnnnn_n'
Cuts at: 1680 2441 3782 1680
Size: 761 1341 1680
Fragments arranged by size:
1680 1341 761
BclI T'GATC_A
Cuts at: 1302 1302
Size: 3782
BfrBI ATG'CAT
Cuts at: 183 183
Size: 3782
BglII A'GATC_T
Cuts at: 1297 3334 1297
Size: 2037 1745
BlpI GC'TnA_GC
Cuts at: 894 894
Size: 3782
Bme1580I G_kGCm'C
Cuts at: 568 1407 1500 2556 568
Size: 839 93 1056 1794
Fragments arranged by size:
1794 1056 839 93
BmrI ACTGGGnnnn_n'
Cuts at: 994 1075 1410 3554 994
Size: 81 335 2144 1222
Fragments arranged by size:
2144 1222 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 3782
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 958 1837 2329 2627 958
Size: 879 492 298 2113
Fragments arranged by size:
2113 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 531 3486 531
Size: 2955 827
BsaAI yAC'GTr
Cuts at: 1135 1135
Size: 3782
BsaBI GATnn'nnATC
Cuts at: 6 1296 3333 6
Size: 1290 2037 455
Fragments arranged by size:
2037 1290 455
BsaHI Gr'CG_yC
Cuts at: 1213 1461 2163 3659 1213
Size: 248 702 1496 1336
Fragments arranged by size:
1496 1336 702 248
BsaWI w'CCGG_w
Cuts at: 971 1492 2170 2444 2591 3002 971
Size: 521 678 274 147 411 1751
Fragments arranged by size:
1751 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 594 624 594
Size: 30 3752
BseYI C'CCAG_C
Cuts at: 1207 1965 2542 3038 1207
Size: 758 577 496 1951
Fragments arranged by size:
1951 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 678 678
Size: 3782
BsiEI CG_ry'CG
Cuts at: 104 1324 1370 2578 104
Size: 1220 46 1208 1308
Fragments arranged by size:
1308 1220 1208 46
BsiHKAI G_wGCw'C
Cuts at: 409 796 1574 1764 2556 409
Size: 387 778 190 792 1635
Fragments arranged by size:
1635 792 778 387 190
Bsp1286I G_dGCh'C
Cuts at: 409 568 796 1407 1500 1574 1764 1826 2556
Size: 159 228 611 93 74 190 62 730
Cuts at: 2556 409
Size: 1635
Fragments arranged by size:
1635 730 611 228 190 159 93 74
62
BspEI T'CCGG_A
Cuts at: 3002 3002
Size: 3782
BspHI T'CATG_A
Cuts at: 2958 2958
Size: 3782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 832 1046 1348 1729 832
Size: 214 302 381 2885
Fragments arranged by size:
2885 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2074 2128 3612 3777 50
Size: 2024 54 1484 165 55
Fragments arranged by size:
2024 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 1694
Size: 3782
BsrFI r'CCGG_y
Cuts at: 1780 1961 2170 1780
Size: 181 209 3392
Fragments arranged by size:
3392 209 181
BsrGI T'GTAC_A
Cuts at: 281 281
Size: 3782
BssSI C'ACGA_G
Cuts at: 2053 2411 2053
Size: 358 3424
BstAPI GCAn_nnn'nTGC
Cuts at: 1159 1159
Size: 3782
BstBI TT'CG_AA
Cuts at: 2143 2143
Size: 3782
BstYI r'GATC_y
Cuts at: 1 217 807 1274 1297 1632 1878 2890 2976
Size: 216 590 467 23 335 246 1012 86
Cuts at: 2976 2988 3334 1
Size: 12 346 449
Fragments arranged by size:
1012 590 467 449 346 335 246 216
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1732 1913 2076 1732
Size: 181 163 3438
Fragments arranged by size:
3438 181 163
BtsI GCAGTG_nn'
Cuts at: 445 1025 445
Size: 580 3202
DraI TTT'AAA
Cuts at: 781 781
Size: 3782
DrdI GACnn_nn'nnGTC
Cuts at: 117 1488 2346 117
Size: 1371 858 1553
Fragments arranged by size:
1553 1371 858
EaeI y'GGCC_r
Cuts at: 288 1367 1541 1932 1959 3304 288
Size: 1079 174 391 27 1345 766
Fragments arranged by size:
1345 1079 766 391 174 27
EagI C'GGCC_G
Cuts at: 1367 1367
Size: 3782
EarI CTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1990 2300 2446 3592 1990
Size: 310 146 1146 2180
Fragments arranged by size:
2180 1146 310 146
EcoICRI GAG'CTC
Cuts at: 794 794
Size: 3782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1607 2039 2786 3054 1607
Size: 432 747 268 2335
Fragments arranged by size:
2335 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 921 921
Size: 3782
EcoRI G'AATT_C
Cuts at: 786 786
Size: 3782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1801 1801
Size: 3782
FspI TGC'GCA
Cuts at: 1563 1563
Size: 3782
HaeII r_GCGC'y
Cuts at: 1216 1464 2486 3465 1216
Size: 248 1022 979 1533
Fragments arranged by size:
1533 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1624 1654 1656 1686 1624
Size: 30 2 30 3720
Fragments arranged by size:
3720 30 30 2
HincII GTy'rAC
Cuts at: 821 821
Size: 3782
KasI G'GCGC_C
Cuts at: 1212 1460 1212
Size: 248 3534
KpnI G_GTAC'C
Cuts at: 802 802
Size: 3782
MluI A'CGCG_T
Cuts at: 3215 3215
Size: 3782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2453 2637 2453
Size: 184 3598
MscI TGG'CCA
Cuts at: 1543 1543
Size: 3782
MslI CAynn'nnrTG
Cuts at: 766 1771 1898 766
Size: 1005 127 2650
Fragments arranged by size:
2650 1005 127
MspA1I CmG'CkG
Cuts at: 368 893 1207 1567 2580 2825 368
Size: 525 314 360 1013 245 1325
Fragments arranged by size:
1325 1013 525 360 314 245
NaeI GCC'GGC
Cuts at: 1963 1963
Size: 3782
NarI GG'CG_CC
Cuts at: 1213 1461 1213
Size: 248 3534
NgoMIV G'CCGG_C
Cuts at: 1961 1961
Size: 3782
NsiI A_TGCA'T
Cuts at: 185 185
Size: 3782
NspI r_CATG'y
Cuts at: 835 1335 2242 835
Size: 500 907 2375
Fragments arranged by size:
2375 907 500
PasI CC'CwG_GG
Cuts at: 378 378
Size: 3782
PciI A'CATG_T
Cuts at: 2238 2238
Size: 3782
PmeI GTTT'AAAC
Cuts at: 781 781
Size: 3782
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 2921
Size: 32 3750
PsiI TTA'TAA
Cuts at: 362 362
Size: 3782
PstI C_TGCA'G
Cuts at: 829 1514 829
Size: 685 3097
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3782
PvuII CAG'CTG
Cuts at: 368 1207 1567 368
Size: 839 360 2583
Fragments arranged by size:
2583 839 360
RsrII CG'GwC_CG
Cuts at: 1977 1977
Size: 3782
SacI G_AGCT'C
Cuts at: 796 796
Size: 3782
SalI G'TCGA_C
Cuts at: 819 819
Size: 3782
SapI GCTCTTCn'nnn_
Cuts at: 1805 2015 1805
Size: 210 3572
SbfI CC_TGCA'GG
Cuts at: 829 829
Size: 3782
ScaI AGT'ACT
Cuts at: 301 3299 301
Size: 2998 784
SfcI C'TryA_G
Cuts at: 33 510 825 1510 2503 2694 33
Size: 477 315 685 993 191 1121
Fragments arranged by size:
1121 993 685 477 315 191
SfoI GGC'GCC
Cuts at: 1214 1462 1214
Size: 248 3534
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3782
SmaI CCC'GGG
Cuts at: 804 804
Size: 3782
SmlI C'TyrA_G
Cuts at: 937 1852 2344 2606 937
Size: 915 492 262 2113
Fragments arranged by size:
2113 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3782
SphI G_CATG'C
Cuts at: 835 1335 835
Size: 500 3282
StyI C'CwwG_G
Cuts at: 259 916 1270 259
Size: 657 354 2771
Fragments arranged by size:
2771 657 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 756 1365 1498 1957 2166 253
Size: 503 609 133 459 209 1869
Fragments arranged by size:
1869 609 503 459 209 133
TatI w'GTAC_w
Cuts at: 281 299 3297 281
Size: 18 2998 766
Fragments arranged by size:
2998 766 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 435 467 583 594 615 626 3299 3331 435
Size: 32 116 11 21 11 2673 32 886
Fragments arranged by size:
2673 886 116 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1579 1579
Size: 3782
XbaI T'CTAG_A
Cuts at: 813 813
Size: 3782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 129 579 1277 129
Size: 450 698 2634
Fragments arranged by size:
2634 698 450
XmaI C'CCGG_G
Cuts at: 802 802
Size: 3782
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI