Restriction map for
pF1KB6907
(Circular) (Six-base) MAPSORT of: pF1KB6907.seq Check: 4011 from: 1 to: 3725
pF1KB6907 3725 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1550 1982 2729 2997 439
Size: 1111 432 747 268 1167
Fragments arranged by size:
1167 1111 747 432 268
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AhdI GACnn_n'nnGTC
Cuts at: 675 675
Size: 3725
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 562 594 562
Size: 32 3693
AlwNI CAG_nnn'CTG
Cuts at: 228 1030 2597 228
Size: 802 1567 1356
Fragments arranged by size:
1567 1356 802
ApaI G_GGCC'C
Cuts at: 361 560 361
Size: 199 3526
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 354 560 620 745 354
Size: 206 60 125 3334
Fragments arranged by size:
3334 206 125 60
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 600 741 1155 1403 1438 600
Size: 141 414 248 35 2887
Fragments arranged by size:
2887 414 248 141 35
BanII G_rGCy'C
Cuts at: 125 361 560 739 1769 125
Size: 236 199 179 1030 2081
Fragments arranged by size:
2081 1030 236 199 179
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 113 837 113
Size: 724 3001
Bme1580I G_kGCm'C
Cuts at: 361 560 1350 1443 2499 361
Size: 199 790 93 1056 1587
Fragments arranged by size:
1587 1056 790 199 93
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 409 385
Size: 24 3701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 503 1078 503
Size: 575 3150
BsaBI GATnn'nnATC
Cuts at: 6 445 1239 3276 6
Size: 439 794 2037 455
Fragments arranged by size:
2037 794 455 439
BsaHI Gr'CG_yC
Cuts at: 515 1156 1404 2106 3602 515
Size: 641 248 702 1496 638
Fragments arranged by size:
1496 702 641 638 248
BsaWI w'CCGG_w
Cuts at: 437 914 1435 2113 2387 2534 2945 437
Size: 477 521 678 274 147 411 1217
Fragments arranged by size:
1217 678 521 477 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 562
Size: 30 3695
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 583 610 178
Size: 405 27 3293
Fragments arranged by size:
3293 405 27
BseYI C'CCAG_C
Cuts at: 273 1150 1908 2485 2981 273
Size: 877 758 577 496 1017
Fragments arranged by size:
1017 877 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 363 363
Size: 3725
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 176 739 1517 1707 2499 176
Size: 563 778 190 792 1402
Fragments arranged by size:
1402 792 778 563 190
Bsp1286I G_dGCh'C
Cuts at: 125 176 361 560 739 1350 1443 1517 1707
Size: 51 185 199 179 611 93 74 190
Cuts at: 1707 1769 2499 125
Size: 62 730 1351
Fragments arranged by size:
1351 730 611 199 190 185 179 93
74 62 51
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstXI CCAn_nnnn'nTGG
Cuts at: 318 318
Size: 3725
BstYI r'GATC_y
Cuts at: 1 218 750 1217 1240 1575 1821 2833 2919
Size: 217 532 467 23 335 246 1012 86
Cuts at: 2919 2931 3277 1
Size: 12 346 449
Fragments arranged by size:
1012 532 467 449 346 335 246 217
86 23 12
BtgI C'CryG_G
Cuts at: 107 590 107
Size: 483 3242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 304 1431 2289 304
Size: 1127 858 1740
Fragments arranged by size:
1740 1127 858
EaeI y'GGCC_r
Cuts at: 284 1310 1484 1875 1902 3247 284
Size: 1026 174 391 27 1345 762
Fragments arranged by size:
1345 1026 762 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 199 1748 1958 199
Size: 1549 210 1966
Fragments arranged by size:
1966 1549 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 409 439 1550 1982 2729 2997 385
Size: 24 30 1111 432 747 268 1113
Fragments arranged by size:
1113 1111 747 432 268 30 24
EcoO109I rG'GnC_Cy
Cuts at: 267 358 557 864 267
Size: 91 199 307 3128
Fragments arranged by size:
3128 307 199 91
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 663 1744 663
Size: 1081 2644
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 1159 1407 2429 3408 1159
Size: 248 1022 979 1476
Fragments arranged by size:
1476 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 460 492 661 693 1567 1597 1599
Size: 32 268 32 169 32 874 30 2
Cuts at: 1599 1629 160
Size: 30 2256
Fragments arranged by size:
2256 874 268 169 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 183 462 764 183
Size: 279 302 3144
Fragments arranged by size:
3144 302 279
HpaI GTT'AAC
Cuts at: 462 462
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 138 154 836 1150 1510 2523 2768 138
Size: 16 682 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 682 360 314 245 16
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NcoI C'CATG_G
Cuts at: 107 107
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PflMI CCAn_nnn'nTGG
Cuts at: 228 228
Size: 3725
PfoI T'CCnGG_A
Cuts at: 212 221 212
Size: 9 3716
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 594 2864 2896 562
Size: 32 2270 32 1391
Fragments arranged by size:
2270 1391 32 32
PspOMI G'GGCC_C
Cuts at: 357 556 357
Size: 199 3526
PstI C_TGCA'G
Cuts at: 772 1457 772
Size: 685 3040
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 138 1150 1510 138
Size: 1012 360 2353
Fragments arranged by size:
2353 1012 360
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 199 1748 1958 199
Size: 1549 210 1966
Fragments arranged by size:
1966 1549 210
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 630 768 1453 2446 2637 33
Size: 597 138 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 191 138
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 356 562 622 747 356
Size: 206 60 125 3334
Fragments arranged by size:
3334 206 125 60
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SnaBI TAC'GTA
Cuts at: 503 503
Size: 3725
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 107 605 859 1213 107
Size: 498 254 354 2619
Fragments arranged by size:
2619 498 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 168 618 682 1308 1441 1900 2109 168
Size: 450 64 626 133 459 209 1784
Fragments arranged by size:
1784 626 459 450 209 133 64
TatI w'GTAC_w
Cuts at: 257 488 3240 257
Size: 231 2752 742
Fragments arranged by size:
2752 742 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 612 1220 612
Size: 608 3117
XmaI C'CCGG_G
Cuts at: 354 560 620 745 354
Size: 206 60 125 3334
Fragments arranged by size:
3334 206 125 60
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI