Restriction map for
pF1KB6909
(Circular) (Six-base) MAPSORT of: pF1KB6909.seq Check: 8486 from: 1 to: 3728
pF1KB6909 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 173 744 173
Size: 571 3157
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 257 694 1033 2600 257
Size: 437 339 1567 1385
Fragments arranged by size:
1567 1385 437 339
ApaI G_GGCC'C
Cuts at: 148 675 688 148
Size: 527 13 3188
Fragments arranged by size:
3188 527 13
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 173 744 1158 1406 1441 173
Size: 571 414 248 35 2460
Fragments arranged by size:
2460 571 414 248 35
BanII G_rGCy'C
Cuts at: 148 304 675 688 742 1772 148
Size: 156 371 13 54 1030 2104
Fragments arranged by size:
2104 1030 371 156 54 13
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 694 728 694
Size: 34 3694
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 337 840 337
Size: 503 3225
Bme1580I G_kGCm'C
Cuts at: 148 592 675 688 1353 1446 2502 148
Size: 444 83 13 665 93 1056 1374
Fragments arranged by size:
1374 1056 665 444 93 83 13
BmrI ACTGGGnnnn_n'
Cuts at: 646 940 1021 1356 3500 646
Size: 294 81 335 2144 874
Fragments arranged by size:
2144 874 335 294 81
BmtI G_CTAG'C
Cuts at: 625 625
Size: 3728
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 366 3432 366
Size: 3066 662
BsaAI yAC'GTr
Cuts at: 270 1081 270
Size: 811 2917
BsaBI GATnn'nnATC
Cuts at: 6 646 1242 3279 6
Size: 640 596 2037 455
Fragments arranged by size:
2037 640 596 455
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 3698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 241 496 561 241
Size: 255 65 3408
Fragments arranged by size:
3408 255 65
BseYI C'CCAG_C
Cuts at: 148 152 674 1153 1911 2488 2984 148
Size: 4 522 479 758 577 496 892
Fragments arranged by size:
892 758 577 522 496 479 4
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 148 304 592 675 688 742 1353 1446 1520
Size: 156 288 83 13 54 611 93 74
Cuts at: 1520 1710 1772 2502 148
Size: 190 62 730 1374
Fragments arranged by size:
1374 730 611 288 190 156 93 83
74 62 54 13
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 486 2020 2074 3558 3723 50
Size: 436 1534 54 1484 165 55
Fragments arranged by size:
1534 1484 436 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 341 469 1726 1907 2116 341
Size: 128 1257 181 209 1953
Fragments arranged by size:
1953 1257 209 181 128
BssHII G'CGCG_C
Cuts at: 332 332
Size: 3728
BssSI C'ACGA_G
Cuts at: 478 1999 2357 478
Size: 1521 358 1849
Fragments arranged by size:
1849 1521 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 368 753 1220 1243 1578 1824 2836 2922
Size: 367 385 467 23 335 246 1012 86
Cuts at: 2922 2934 3280 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 385 367 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 584
Size: 3728
BtgI C'CryG_G
Cuts at: 599 599
Size: 3728
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 244 314 377 971 244
Size: 70 63 594 3001
Fragments arranged by size:
3001 594 70 63
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 259 311 1313 1487 1878 1905 3250 259
Size: 52 1002 174 391 27 1345 737
Fragments arranged by size:
1345 1002 737 391 174 52 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 196 1751 1961 196
Size: 1555 210 1963
Fragments arranged by size:
1963 1555 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 144 145 156 606 684 867 144
Size: 1 11 450 78 183 3005
Fragments arranged by size:
3005 450 183 78 11 1
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 120 1162 1410 2432 3411 120
Size: 1042 248 1022 979 437
Fragments arranged by size:
1042 1022 979 437 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 1570 1600 1602 1632 217
Size: 32 1321 30 2 30 2313
Fragments arranged by size:
2313 1321 32 30 30 2
HincII GTy'rAC
Cuts at: 411 767 411
Size: 356 3372
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 177 748 177
Size: 571 3157
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 659 2399 2583 659
Size: 1740 184 1804
Fragments arranged by size:
1804 1740 184
MscI TGG'CCA
Cuts at: 261 1489 261
Size: 1228 2500
MslI CAynn'nnrTG
Cuts at: 1717 1844 1717
Size: 127 3601
MspA1I CmG'CkG
Cuts at: 152 257 356 389 839 1153 1513 2526 2771
Size: 105 99 33 450 314 360 1013 245
Cuts at: 2771 152
Size: 1109
Fragments arranged by size:
1109 1013 450 360 314 245 105 99
33
NaeI GCC'GGC
Cuts at: 343 1909 343
Size: 1566 2162
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NgoMIV G'CCGG_C
Cuts at: 341 1907 341
Size: 1566 2162
NheI G'CTAG_C
Cuts at: 621 621
Size: 3728
NspI r_CATG'y
Cuts at: 781 1281 2188 781
Size: 500 907 2321
Fragments arranged by size:
2321 907 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PpuMI rG'GwC_Cy
Cuts at: 156 156
Size: 3728
PspOMI G'GGCC_C
Cuts at: 144 671 684 144
Size: 527 13 3188
Fragments arranged by size:
3188 527 13
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 152 257 1153 1513 152
Size: 105 896 360 2367
Fragments arranged by size:
2367 896 360 105
RsrII CG'GwC_CG
Cuts at: 384 1923 384
Size: 1539 2189
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SanDI GG'GwC_CC
Cuts at: 156 156
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 324 771 1456 2449 2640 33
Size: 291 447 685 993 191 1121
Fragments arranged by size:
1121 993 685 447 291 191
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StuI AGG'CCT
Cuts at: 202 202
Size: 3728
StyI C'CwwG_G
Cuts at: 446 719 862 1216 446
Size: 273 143 354 2958
Fragments arranged by size:
2958 354 273 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 574 1311 1444 1903 2112 127
Size: 447 737 133 459 209 1743
Fragments arranged by size:
1743 737 459 447 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 1223 453
Size: 770 2958
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI