Restriction map for
pF1KB6910
(Circular) (Six-base) MAPSORT of: pF1KB6910.seq Check: 9628 from: 1 to: 3785
pF1KB6910 3785 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1610 2042 2789 3057 389
Size: 1221 432 747 268 1117
Fragments arranged by size:
1221 1117 747 432 268
AflII C'TTAA_G
Cuts at: 424 424
Size: 3785
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 524 524
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 151 163 542 551 694 789 3138 3394 151
Size: 12 379 9 143 95 2349 256 542
Fragments arranged by size:
2349 542 379 256 143 95 12 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 805 805
Size: 3785
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 613 799 1829 613
Size: 186 1030 2569
Fragments arranged by size:
2569 1030 186
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BbsI GAAGACnn'nnnn_
Cuts at: 721 721
Size: 3785
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 672 1410 1503 2559 672
Size: 738 93 1056 1898
Fragments arranged by size:
1898 1056 738 93
BmrI ACTGGGnnnn_n'
Cuts at: 997 1078 1413 3557 997
Size: 81 335 2144 1225
Fragments arranged by size:
2144 1225 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 961 1840 2332 2630 700
Size: 261 879 492 298 1855
Fragments arranged by size:
1855 879 492 298 261
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1210 1968 2545 3041 1210
Size: 758 577 496 1954
Fragments arranged by size:
1954 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1327 1373 2581 104
Size: 1223 46 1208 1308
Fragments arranged by size:
1308 1223 1208 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
BsmI GAATG_Cn'
Cuts at: 483 483
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 613 672 799 1410 1503 1577 1767 1829 2559
Size: 59 127 611 93 74 190 62 730
Cuts at: 2559 613
Size: 1839
Fragments arranged by size:
1839 730 611 190 127 93 74 62
59
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 2961 2961
Size: 3785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 835 1049 1351 1732 835
Size: 214 302 381 2888
Fragments arranged by size:
2888 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 1783 1964 2173 1783
Size: 181 209 3395
Fragments arranged by size:
3395 209 181
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 1162 1162
Size: 3785
BstBI TT'CG_AA
Cuts at: 546 2146 546
Size: 1600 2185
BstYI r'GATC_y
Cuts at: 1 420 810 1277 1300 1635 1881 2893 2979
Size: 419 390 467 23 335 246 1012 86
Cuts at: 2979 2991 3337 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 419 390 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 767 107
Size: 660 3125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 1370 1544 1935 1962 3307 1370
Size: 174 391 27 1345 1848
Fragments arranged by size:
1848 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1610 2042 2789 3057 389
Size: 1221 432 747 268 1117
Fragments arranged by size:
1221 1117 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 684 924 684
Size: 240 3545
EcoRI G'AATT_C
Cuts at: 789 789
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 547 1804 366
Size: 181 1257 2347
Fragments arranged by size:
2347 1257 181
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 1219 1467 2489 3468 1219
Size: 248 1022 979 1536
Fragments arranged by size:
1536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1627 1657 1659 1689 1627
Size: 30 2 30 3723
Fragments arranged by size:
3723 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
HindIII A'AGCT_T
Cuts at: 755 755
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 635 1774 1901 635
Size: 1139 127 2519
Fragments arranged by size:
2519 1139 127
MspA1I CmG'CkG
Cuts at: 896 1210 1570 2583 2828 896
Size: 314 360 1013 245 1853
Fragments arranged by size:
1853 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 107 107
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PfoI T'CCnGG_A
Cuts at: 416 416
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PsiI TTA'TAA
Cuts at: 776 776
Size: 3785
PstI C_TGCA'G
Cuts at: 503 513 832 1517 503
Size: 10 319 685 2771
Fragments arranged by size:
2771 685 319 10
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 1210 1570 1210
Size: 360 3425
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 1808 2018 1808
Size: 210 3575
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 401 3302 401
Size: 2901 884
SfcI C'TryA_G
Cuts at: 33 499 509 828 1513 2506 2697 33
Size: 466 10 319 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 319 191 10
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 424 679 940 1855 2347 2609 424
Size: 255 261 915 492 262 1600
Fragments arranged by size:
1600 915 492 262 261 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
SspI AAT'ATT
Cuts at: 436 588 436
Size: 152 3633
StyI C'CwwG_G
Cuts at: 107 919 1273 107
Size: 812 354 2619
Fragments arranged by size:
2619 812 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 399 3300 399
Size: 2901 884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
XmnI GAAnn'nnTTC
Cuts at: 163 163
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI