Restriction map for
pF1KB6913
(Circular) (Six-base) MAPSORT of: pF1KB6913.seq Check: 4059 from: 1 to: 3728
pF1KB6913 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 1033 2600 1033
Size: 1567 2161
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 457 732 3081 3337 457
Size: 275 2349 256 848
Fragments arranged by size:
2349 848 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 744 1158 1406 1441 744
Size: 414 248 35 3031
Fragments arranged by size:
3031 414 248 35
BanII G_rGCy'C
Cuts at: 742 1772 742
Size: 1030 2698
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 446 1243 3280 446
Size: 797 2037 894
Fragments arranged by size:
2037 894 797
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 598 904 1783 2275 2573 165
Size: 433 306 879 492 298 1320
Fragments arranged by size:
1320 879 492 433 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 162 379 162
Size: 217 3511
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 742 1353 1446 1520 1710 1772 2502 742
Size: 611 93 74 190 62 730 1968
Fragments arranged by size:
1968 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 312 2904 312
Size: 2592 1136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 115 556 778 992 1294 1675 115
Size: 441 222 214 302 381 2168
Fragments arranged by size:
2168 441 381 302 222 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 132 1726 1907 2116 132
Size: 1594 181 209 1744
Fragments arranged by size:
1744 1594 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 697 1105 697
Size: 408 3320
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 446 753 1220 1243 1578 1824 2836 2922
Size: 445 307 467 23 335 246 1012 86
Cuts at: 2922 2934 3280 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 307 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 1313 1487 1878 1905 3250 1313
Size: 174 391 27 1345 1791
Fragments arranged by size:
1791 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 389 506 1751 1961 389
Size: 117 1245 210 2156
Fragments arranged by size:
2156 1245 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 457 732 457
Size: 275 3453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 1162 1410 2432 3411 1162
Size: 248 1022 979 1479
Fragments arranged by size:
1479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 642 767 642
Size: 125 3603
HindIII A'AGCT_T
Cuts at: 338 338
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 317 1717 1844 317
Size: 1400 127 2201
Fragments arranged by size:
2201 1400 127
MspA1I CmG'CkG
Cuts at: 389 653 839 1153 1513 2526 2771 389
Size: 264 186 314 360 1013 245 1346
Fragments arranged by size:
1346 1013 360 314 264 245 186
NaeI GCC'GGC
Cuts at: 134 1909 134
Size: 1775 1953
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NgoMIV G'CCGG_C
Cuts at: 132 1907 132
Size: 1775 1953
NspI r_CATG'y
Cuts at: 531 781 1281 2188 531
Size: 250 500 907 2071
Fragments arranged by size:
2071 907 500 250
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 389 653 1153 1513 389
Size: 264 500 360 2604
Fragments arranged by size:
2604 500 360 264
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 771 1456 2449 2640 33
Size: 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 191
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 144 613 883 1798 2290 2552 144
Size: 469 270 915 492 262 1320
Fragments arranged by size:
1320 915 492 469 270 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
SspI AAT'ATT
Cuts at: 372 372
Size: 3728
StyI C'CwwG_G
Cuts at: 434 862 1216 434
Size: 428 354 2946
Fragments arranged by size:
2946 428 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1311 1444 1903 2112 1311
Size: 133 459 209 2927
Fragments arranged by size:
2927 459 209 133
TatI w'GTAC_w
Cuts at: 398 3243 398
Size: 2845 883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 375 407 3245 3277 202
Size: 32 141 32 2838 32 653
Fragments arranged by size:
2838 653 141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
XmnI GAAnn'nnTTC
Cuts at: 574 574
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI