Restriction map for
pF1KB6915
(Circular) (Six-base) MAPSORT of: pF1KB6915.seq Check: 5843 from: 1 to: 3728
pF1KB6915 3728 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 417 744 417
Size: 327 3401
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
AflII C'TTAA_G
Cuts at: 427 427
Size: 3728
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 1033 2600 1033
Size: 1567 2161
ApaI G_GGCC'C
Cuts at: 122 122
Size: 3728
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 460 20
Size: 440 3288
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
AvrII C'CTAG_G
Cuts at: 282 282
Size: 3728
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 417 744 1158 1406 1441 417
Size: 327 414 248 35 2704
Fragments arranged by size:
2704 414 327 248 35
BanII G_rGCy'C
Cuts at: 122 742 1772 122
Size: 620 1030 2078
Fragments arranged by size:
2078 1030 620
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BbsI GAAGACnn'nnnn_
Cuts at: 225 317 348 628 225
Size: 92 31 280 3325
Fragments arranged by size:
3325 280 92 31
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 122 227 1353 1446 2502 122
Size: 105 1126 93 1056 1348
Fragments arranged by size:
1348 1126 1056 105 93
BmgBI CAC'GTC
Cuts at: 176 176
Size: 3728
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 121 904 1783 2275 2573 121
Size: 783 879 492 298 1276
Fragments arranged by size:
1276 879 783 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 222 1081 222
Size: 859 2869
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 203 1159 1407 2109 3605 203
Size: 956 248 702 1496 326
Fragments arranged by size:
1496 956 702 326 248
BsaWI w'CCGG_w
Cuts at: 420 917 1438 2116 2390 2537 2948 420
Size: 497 521 678 274 147 411 1200
Fragments arranged by size:
1200 678 521 497 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 424 229
Size: 195 3533
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 302 1270 1316 2524 104
Size: 198 968 46 1208 1308
Fragments arranged by size:
1308 1208 968 198 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 122 227 742 1353 1446 1520 1710 1772 2502
Size: 105 515 611 93 74 190 62 730
Cuts at: 2502 122
Size: 1348
Fragments arranged by size:
1348 730 611 515 190 105 93 74
62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 185 2904 185
Size: 2719 1009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssHII G'CGCG_C
Cuts at: 125 125
Size: 3728
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 176 1105 176
Size: 929 2799
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 753 1220 1243 1578 1824 2836 2922 2934
Size: 752 467 23 335 246 1012 86 12
Cuts at: 2934 3280 1
Size: 346 449
Fragments arranged by size:
1012 752 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 260 107
Size: 153 3575
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 299 1313 1487 1878 1905 3250 299
Size: 1014 174 391 27 1345 777
Fragments arranged by size:
1345 1014 777 391 174 27
EagI C'GGCC_G
Cuts at: 299 1313 299
Size: 1014 2714
EarI CTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 1162 1410 2432 3411 1162
Size: 248 1022 979 1479
Fragments arranged by size:
1479 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 421 748 421
Size: 327 3401
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 1717 1844 1717
Size: 127 3601
MspA1I CmG'CkG
Cuts at: 839 1153 1513 2526 2771 839
Size: 314 360 1013 245 1796
Fragments arranged by size:
1796 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NcoI C'CATG_G
Cuts at: 107 107
Size: 3728
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 581 781 1281 2188 581
Size: 200 500 907 2121
Fragments arranged by size:
2121 907 500 200
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PfoI T'CCnGG_A
Cuts at: 209 209
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PshAI GACnn'nnGTC
Cuts at: 352 352
Size: 3728
PspOMI G'GGCC_C
Cuts at: 118 118
Size: 3728
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 1153 1513 1153
Size: 360 3368
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 771 1456 2449 2640 33
Size: 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 191
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 136 427 883 1798 2290 2552 136
Size: 291 456 915 492 262 1312
Fragments arranged by size:
1312 915 492 456 291 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 581 781 1281 581
Size: 200 500 3028
Fragments arranged by size:
3028 500 200
StuI AGG'CCT
Cuts at: 330 330
Size: 3728
StyI C'CwwG_G
Cuts at: 107 282 862 1216 107
Size: 175 580 354 2619
Fragments arranged by size:
2619 580 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 364 1311 1444 1903 2112 364
Size: 947 133 459 209 1980
Fragments arranged by size:
1980 947 459 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI