Restriction map for
pF1KB6917
(Circular) (Six-base) MAPSORT of: pF1KB6917.seq Check: 6715 from: 1 to: 3728
pF1KB6917 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 632 632
Size: 3728
AccI GT'mk_AC
Cuts at: 332 766 332
Size: 434 3294
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1553 1985 2732 3000 566
Size: 987 432 747 268 1294
Fragments arranged by size:
1294 987 747 432 268
AfeI AGC'GCT
Cuts at: 688 688
Size: 3728
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 615 1033 2600 615
Size: 418 1567 1743
Fragments arranged by size:
1743 1567 418
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 593 748 593
Size: 155 3573
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 114 267 744 1158 1406 1441 114
Size: 153 477 414 248 35 2401
Fragments arranged by size:
2401 477 414 248 153 35
BanII G_rGCy'C
Cuts at: 742 1772 742
Size: 1030 2698
BbeI G_GCGC'C
Cuts at: 118 271 1162 1410 118
Size: 153 891 248 2436
Fragments arranged by size:
2436 891 248 153
BbsI GAAGACnn'nnnn_
Cuts at: 554 554
Size: 3728
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglI GCCn_nnn'nGGC
Cuts at: 112 247 112
Size: 135 3593
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmgBI CAC'GTC
Cuts at: 344 344
Size: 3728
BmrI ACTGGGnnnn_n'
Cuts at: 421 492 940 1021 1356 3500 421
Size: 71 448 81 335 2144 649
Fragments arranged by size:
2144 649 448 335 81 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 2095 318
Size: 1777 1951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 115 256 268 299 1159 1407 2109 3605 115
Size: 141 12 31 860 248 702 1496 238
Fragments arranged by size:
1496 860 702 248 238 141 31 12
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 550
Size: 3728
BseYI C'CCAG_C
Cuts at: 425 648 1153 1911 2488 2984 425
Size: 223 505 758 577 496 1169
Fragments arranged by size:
1169 758 577 505 496 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 610 588
Size: 22 3706
BsiEI CG_ry'CG
Cuts at: 104 203 242 316 1270 1316 2524 104
Size: 99 39 74 954 46 1208 1308
Fragments arranged by size:
1308 1208 954 99 74 46 39
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 227 227
Size: 3728
Bsp1286I G_dGCh'C
Cuts at: 742 1353 1446 1520 1710 1772 2502 742
Size: 611 93 74 190 62 730 1968
Fragments arranged by size:
1968 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 473 632 778 992 1294 1675 473
Size: 159 146 214 302 381 2526
Fragments arranged by size:
2526 381 302 214 159 146
BsrBI CCG'CTC
Cuts at: 50 126 2020 2074 3558 3723 50
Size: 76 1894 54 1484 165 55
Fragments arranged by size:
1894 1484 165 76 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssHII G'CGCG_C
Cuts at: 170 172 170
Size: 2 3726
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 753 1220 1243 1578 1824 2836 2922 2934
Size: 752 467 23 335 246 1012 86 12
Cuts at: 2934 3280 1
Size: 346 449
Fragments arranged by size:
1012 752 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 138 107
Size: 31 3697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DraIII CAC_nnn'GTG
Cuts at: 611 611
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 239 313 446 1313 1487 1878 1905 3250 239
Size: 74 133 867 174 391 27 1345 717
Fragments arranged by size:
1345 867 717 391 174 133 74 27
EagI C'GGCC_G
Cuts at: 239 313 1313 239
Size: 74 1000 2654
Fragments arranged by size:
2654 1000 74
EarI CTCTTCn'nnn_
Cuts at: 157 1751 1961 157
Size: 1594 210 1924
Fragments arranged by size:
1924 1594 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 243 1936 2246 2392 3538 243
Size: 1693 310 146 1146 433
Fragments arranged by size:
1693 1146 433 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1553 1985 2732 3000 566
Size: 987 432 747 268 1294
Fragments arranged by size:
1294 987 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 306 306
Size: 3728
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 678 1747 678
Size: 1069 2659
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 118 271 690 1162 1410 2432 3411 118
Size: 153 419 472 248 1022 979 435
Fragments arranged by size:
1022 979 472 435 419 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 333 767 333
Size: 434 3294
KasI G'GCGC_C
Cuts at: 114 267 1158 1406 114
Size: 153 891 248 2436
Fragments arranged by size:
2436 891 248 153
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 1717 1844 1717
Size: 127 3601
MspA1I CmG'CkG
Cuts at: 425 839 1153 1513 2526 2771 425
Size: 414 314 360 1013 245 1382
Fragments arranged by size:
1382 1013 414 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 115 268 1159 1407 115
Size: 153 891 248 2436
Fragments arranged by size:
2436 891 248 153
NcoI C'CATG_G
Cuts at: 107 107
Size: 3728
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NotI GC'GGCC_GC
Cuts at: 239 239
Size: 3728
NruI TCG'CGA
Cuts at: 404 404
Size: 3728
NspI r_CATG'y
Cuts at: 602 781 1281 2188 602
Size: 179 500 907 2142
Fragments arranged by size:
2142 907 500 179
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PflMI CCAn_nnn'nTGG
Cuts at: 444 444
Size: 3728
PfoI T'CCnGG_A
Cuts at: 304 304
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 425 1153 1513 425
Size: 728 360 2640
Fragments arranged by size:
2640 728 360
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SalI G'TCGA_C
Cuts at: 331 765 331
Size: 434 3294
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 771 1456 2449 2640 33
Size: 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 247 247
Size: 3728
SfoI GGC'GCC
Cuts at: 116 269 1160 1408 116
Size: 153 891 248 2436
Fragments arranged by size:
2436 891 248 153
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 595 750 595
Size: 155 3573
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 602 781 1281 602
Size: 179 500 3049
Fragments arranged by size:
3049 500 179
StyI C'CwwG_G
Cuts at: 107 862 1216 107
Size: 755 354 2619
Fragments arranged by size:
2619 755 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 593 1311 1444 1903 2112 217
Size: 376 718 133 459 209 1833
Fragments arranged by size:
1833 718 459 376 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 593 748 593
Size: 155 3573
XmnI GAAnn'nnTTC
Cuts at: 151 151
Size: 3728
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI