Restriction map for
pF1KB6918
(Circular) (Six-base) MAPSORT of: pF1KB6918.seq Check: 2834 from: 1 to: 3788
pF1KB6918 3788 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 654 804 654
Size: 150 3638
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 689 689
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 495 516 685 792 3141 3397 495
Size: 21 169 107 2349 256 886
Fragments arranged by size:
2349 886 256 169 107 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 654 804 1218 1466 1501 654
Size: 150 414 248 35 2941
Fragments arranged by size:
2941 414 248 150 35
BanII G_rGCy'C
Cuts at: 289 802 1832 289
Size: 513 1030 2245
Fragments arranged by size:
2245 1030 513
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 292 1000 1081 1416 3560 292
Size: 708 81 335 2144 520
Fragments arranged by size:
2144 708 520 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 728
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 964 1843 2335 2633 324
Size: 640 879 492 298 1479
Fragments arranged by size:
1479 879 640 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1213 1971 2548 3044 1213
Size: 758 577 496 1957
Fragments arranged by size:
1957 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 802 1580 1770 2562 802
Size: 778 190 792 2028
Fragments arranged by size:
2028 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 289 802 1413 1506 1580 1770 1832 2562 289
Size: 513 611 93 74 190 62 730 1515
Fragments arranged by size:
1515 730 611 513 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1052 1354 1735 838
Size: 214 302 381 2891
Fragments arranged by size:
2891 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 671 1700 671
Size: 1029 2759
BsrFI r'CCGG_y
Cuts at: 1786 1967 2176 1786
Size: 181 209 3398
Fragments arranged by size:
3398 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 1165 1165
Size: 3788
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 444 444
Size: 3788
BstYI r'GATC_y
Cuts at: 1 181 813 1280 1303 1638 1884 2896 2982
Size: 180 632 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 632 467 449 346 335 246 180
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 533 533
Size: 3788
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 1031 1031
Size: 3788
DraI TTT'AAA
Cuts at: 236 787 236
Size: 551 3237
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 480 1373 1547 1938 1965 3310 480
Size: 893 174 391 27 1345 958
Fragments arranged by size:
1345 958 893 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 172 581 1811 2021 172
Size: 409 1230 210 1939
Fragments arranged by size:
1939 1230 409 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1613 2045 2792 3060 728
Size: 885 432 747 268 1456
Fragments arranged by size:
1456 885 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 537 927 537
Size: 390 3398
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
EcoRV GAT'ATC
Cuts at: 114 114
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 725 757 1630 1660 1662 1692 364
Size: 32 329 32 873 30 2 30 2460
Fragments arranged by size:
2460 873 329 32 32 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 658 808 658
Size: 150 3638
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2459 2643 2459
Size: 184 3604
MscI TGG'CCA
Cuts at: 482 1549 482
Size: 1067 2721
MslI CAynn'nnrTG
Cuts at: 247 1777 1904 247
Size: 1530 127 2131
Fragments arranged by size:
2131 1530 127
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1969 1969
Size: 3788
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 1967 1967
Size: 3788
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 331 331
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PpuMI rG'GwC_Cy
Cuts at: 537 537
Size: 3788
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 831 1516 2509 2700 33
Size: 798 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 191
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 303 943 1858 2350 2612 303
Size: 640 915 492 262 1479
Fragments arranged by size:
1479 915 640 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StuI AGG'CCT
Cuts at: 262 262
Size: 3788
StyI C'CwwG_G
Cuts at: 107 510 922 1276 107
Size: 403 412 354 2619
Fragments arranged by size:
2619 412 403 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI