Restriction map for
pF1KB6919
(Circular) (Six-base) MAPSORT of: pF1KB6919.seq Check: 4334 from: 1 to: 3764
pF1KB6919 3764 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 579 579
Size: 3764
AccI GT'mk_AC
Cuts at: 802 802
Size: 3764
Acc65I G'GTAC_C
Cuts at: 161 780 161
Size: 619 3145
AclI AA'CG_TT
Cuts at: 3604 3604
Size: 3764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1589 2021 2768 3036 1589
Size: 432 747 268 2317
Fragments arranged by size:
2317 747 432 268
AflIII A'CryG_T
Cuts at: 1116 2220 3197 1116
Size: 1104 977 1683
Fragments arranged by size:
1683 1104 977
AgeI A'CCGG_T
Cuts at: 2152 2152
Size: 3764
AlwNI CAG_nnn'CTG
Cuts at: 130 1069 2636 130
Size: 939 1567 1258
Fragments arranged by size:
1567 1258 939
ApaLI G'TGCA_C
Cuts at: 2534 2534
Size: 3764
ApoI r'AATT_y
Cuts at: 768 3117 3373 768
Size: 2349 256 1159
Fragments arranged by size:
2349 1159 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
AvaI C'yCGr_G
Cuts at: 784 784
Size: 3764
BamHI G'GATC_C
Cuts at: 149 789 149
Size: 640 3124
BanI G'GyrC_C
Cuts at: 161 780 1194 1442 1477 161
Size: 619 414 248 35 2448
Fragments arranged by size:
2448 619 414 248 35
BanII G_rGCy'C
Cuts at: 449 778 1808 449
Size: 329 1030 2405
Fragments arranged by size:
2405 1030 329
BbeI G_GCGC'C
Cuts at: 1198 1446 1198
Size: 248 3516
BbsI GAAGACnn'nnnn_
Cuts at: 259 259
Size: 3764
BciVI GTATCCnnnnn_n'
Cuts at: 670 1662 2423 3764 670
Size: 992 761 1341 670
Fragments arranged by size:
1341 992 761 670
BclI T'GATC_A
Cuts at: 1284 1284
Size: 3764
BglII A'GATC_T
Cuts at: 314 1279 3316 314
Size: 965 2037 762
Fragments arranged by size:
2037 965 762
BlpI GC'TnA_GC
Cuts at: 443 876 443
Size: 433 3331
Bme1580I G_kGCm'C
Cuts at: 468 1389 1482 2538 468
Size: 921 93 1056 1694
Fragments arranged by size:
1694 1056 921 93
BmrI ACTGGGnnnn_n'
Cuts at: 241 976 1057 1392 3536 241
Size: 735 81 335 2144 469
Fragments arranged by size:
2144 735 469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 3764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 557 280
Size: 277 3487
Bpu10I CC'TnA_GC
Cuts at: 401 401
Size: 3764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 940 1819 2311 2609 433
Size: 507 879 492 298 1588
Fragments arranged by size:
1588 879 507 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3468 3468
Size: 3764
BsaAI yAC'GTr
Cuts at: 1117 1117
Size: 3764
BsaBI GATnn'nnATC
Cuts at: 6 1278 3315 6
Size: 1272 2037 455
Fragments arranged by size:
2037 1272 455
BsaHI Gr'CG_yC
Cuts at: 1195 1443 2145 3641 1195
Size: 248 702 1496 1318
Fragments arranged by size:
1496 1318 702 248
BsaWI w'CCGG_w
Cuts at: 953 1474 2152 2426 2573 2984 953
Size: 521 678 274 147 411 1733
Fragments arranged by size:
1733 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1189 1947 2524 3020 1189
Size: 758 577 496 1933
Fragments arranged by size:
1933 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 3764
BsiEI CG_ry'CG
Cuts at: 104 1306 1352 2560 104
Size: 1202 46 1208 1308
Fragments arranged by size:
1308 1208 1202 46
BsiHKAI G_wGCw'C
Cuts at: 449 778 1556 1746 2538 449
Size: 329 778 190 792 1675
Fragments arranged by size:
1675 792 778 329 190
BsmI GAATG_Cn'
Cuts at: 520 520
Size: 3764
BsmBI CGTCTCn'nnnn_
Cuts at: 715 715
Size: 3764
Bsp1286I G_dGCh'C
Cuts at: 449 468 778 1389 1482 1556 1746 1808 2538
Size: 19 310 611 93 74 190 62 730
Cuts at: 2538 449
Size: 1675
Fragments arranged by size:
1675 730 611 310 190 93 74 62
19
BspEI T'CCGG_A
Cuts at: 2984 2984
Size: 3764
BspHI T'CATG_A
Cuts at: 2940 2940
Size: 3764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 579 814 1028 1330 1711 173
Size: 406 235 214 302 381 2226
Fragments arranged by size:
2226 406 381 302 235 214
BsrBI CCG'CTC
Cuts at: 50 2056 2110 3594 3759 50
Size: 2006 54 1484 165 55
Fragments arranged by size:
2006 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1676 1676
Size: 3764
BsrFI r'CCGG_y
Cuts at: 179 1762 1943 2152 179
Size: 1583 181 209 1791
Fragments arranged by size:
1791 1583 209 181
BsrGI T'GTAC_A
Cuts at: 593 593
Size: 3764
BssSI C'ACGA_G
Cuts at: 2035 2393 2035
Size: 358 3406
BstAPI GCAn_nnn'nTGC
Cuts at: 130 414 1141 130
Size: 284 727 2753
Fragments arranged by size:
2753 727 284
BstBI TT'CG_AA
Cuts at: 2125 2125
Size: 3764
BstYI r'GATC_y
Cuts at: 1 149 314 789 1256 1279 1614 1860 2872
Size: 148 165 475 467 23 335 246 1012
Cuts at: 2872 2958 2970 3316 1
Size: 86 12 346 449
Fragments arranged by size:
1012 475 467 449 346 335 246 165
148 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 157 157
Size: 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1714 1895 2058 1714
Size: 181 163 3420
Fragments arranged by size:
3420 181 163
BtsI GCAGTG_nn'
Cuts at: 395 1007 395
Size: 612 3152
DraI TTT'AAA
Cuts at: 763 763
Size: 3764
DrdI GACnn_nn'nnGTC
Cuts at: 367 1470 2328 367
Size: 1103 858 1803
Fragments arranged by size:
1803 1103 858
EaeI y'GGCC_r
Cuts at: 1349 1523 1914 1941 3286 1349
Size: 174 391 27 1345 1827
Fragments arranged by size:
1827 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1349 1349
Size: 3764
EarI CTCTTCn'nnn_
Cuts at: 572 625 631 1787 1997 572
Size: 53 6 1156 210 2339
Fragments arranged by size:
2339 1156 210 53 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1972 2282 2428 3574 1972
Size: 310 146 1146 2162
Fragments arranged by size:
2162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 447 776 447
Size: 329 3435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 557 1589 2021 2768 3036 280
Size: 277 1032 432 747 268 1008
Fragments arranged by size:
1032 1008 747 432 277 268
EcoO109I rG'GnC_Cy
Cuts at: 903 903
Size: 3764
EcoRI G'AATT_C
Cuts at: 768 768
Size: 3764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 1783 717
Size: 1066 2698
FspI TGC'GCA
Cuts at: 1545 1545
Size: 3764
HaeII r_GCGC'y
Cuts at: 1198 1446 2468 3447 1198
Size: 248 1022 979 1515
Fragments arranged by size:
1515 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1606 1636 1638 1668 1606
Size: 30 2 30 3702
Fragments arranged by size:
3702 30 30 2
HincII GTy'rAC
Cuts at: 803 803
Size: 3764
KasI G'GCGC_C
Cuts at: 1194 1442 1194
Size: 248 3516
KpnI G_GTAC'C
Cuts at: 165 784 165
Size: 619 3145
MluI A'CGCG_T
Cuts at: 3197 3197
Size: 3764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2435 2619 2435
Size: 184 3580
MscI TGG'CCA
Cuts at: 1525 1525
Size: 3764
MslI CAynn'nnrTG
Cuts at: 1753 1880 1753
Size: 127 3637
MspA1I CmG'CkG
Cuts at: 219 267 875 1189 1549 2562 2807 219
Size: 48 608 314 360 1013 245 1176
Fragments arranged by size:
1176 1013 608 360 314 245 48
NaeI GCC'GGC
Cuts at: 1945 1945
Size: 3764
NarI GG'CG_CC
Cuts at: 1195 1443 1195
Size: 248 3516
NgoMIV G'CCGG_C
Cuts at: 1943 1943
Size: 3764
NspI r_CATG'y
Cuts at: 817 1317 2224 817
Size: 500 907 2357
Fragments arranged by size:
2357 907 500
PciI A'CATG_T
Cuts at: 2220 2220
Size: 3764
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 3764
PmeI GTTT'AAAC
Cuts at: 763 763
Size: 3764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 2935 2903
Size: 32 3732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 169 201 169
Size: 32 3732
PstI C_TGCA'G
Cuts at: 170 811 1496 170
Size: 641 685 2438
Fragments arranged by size:
2438 685 641
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3764
PvuII CAG'CTG
Cuts at: 267 1189 1549 267
Size: 922 360 2482
Fragments arranged by size:
2482 922 360
RsrII CG'GwC_CG
Cuts at: 1959 1959
Size: 3764
SacI G_AGCT'C
Cuts at: 449 778 449
Size: 329 3435
SalI G'TCGA_C
Cuts at: 801 801
Size: 3764
SapI GCTCTTCn'nnn_
Cuts at: 1787 1997 1787
Size: 210 3554
SbfI CC_TGCA'GG
Cuts at: 170 811 170
Size: 641 3123
ScaI AGT'ACT
Cuts at: 613 3281 613
Size: 2668 1096
SfcI C'TryA_G
Cuts at: 33 166 186 214 525 647 807 1492 2485
Size: 133 20 28 311 122 160 685 993
Cuts at: 2485 2676 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 311 191 160 133 122
28 20
SfoI GGC'GCC
Cuts at: 1196 1444 1196
Size: 248 3516
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3764
SmaI CCC'GGG
Cuts at: 786 786
Size: 3764
SmlI C'TyrA_G
Cuts at: 448 919 1834 2326 2588 448
Size: 471 915 492 262 1624
Fragments arranged by size:
1624 915 492 471 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3764
SphI G_CATG'C
Cuts at: 817 1317 817
Size: 500 3264
StyI C'CwwG_G
Cuts at: 898 1252 898
Size: 354 3410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 1480 1939 2148 1347
Size: 133 459 209 2963
Fragments arranged by size:
2963 459 209 133
TatI w'GTAC_w
Cuts at: 593 611 3279 593
Size: 18 2668 1078
Fragments arranged by size:
2668 1078 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3281
Size: 32 3732
Tth111I GACn'n_nGTC
Cuts at: 1561 1561
Size: 3764
XbaI T'CTAG_A
Cuts at: 795 795
Size: 3764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1259 1259
Size: 3764
XmaI C'CCGG_G
Cuts at: 784 784
Size: 3764
XmnI GAAnn'nnTTC
Cuts at: 697 697
Size: 3764
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BseRI BsiWI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI