Restriction map for
pF1KB6920
(Circular) (Six-base) MAPSORT of: pF1KB6920.seq Check: 6431 from: 1 to: 3791
pF1KB6920 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 277 795 3144 3400 277
Size: 518 2349 256 668
Fragments arranged by size:
2349 668 518 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 807 1221 1469 1504 807
Size: 414 248 35 3094
Fragments arranged by size:
3094 414 248 35
BanII G_rGCy'C
Cuts at: 805 1835 805
Size: 1030 2761
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 1311 1311
Size: 3791
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 252 903 252
Size: 651 3140
Bme1580I G_kGCm'C
Cuts at: 1416 1509 2565 1416
Size: 93 1056 2642
Fragments arranged by size:
2642 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 252 1003 1084 1419 3563 252
Size: 751 81 335 2144 480
Fragments arranged by size:
2144 751 480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
Bpu10I CC'TnA_GC
Cuts at: 509 509
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 751 967 1846 2338 2636 383
Size: 368 216 879 492 298 1538
Fragments arranged by size:
1538 879 492 368 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 335 3495 335
Size: 3160 631
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 574 1305 3342 6
Size: 568 731 2037 455
Fragments arranged by size:
2037 731 568 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 287 317 287
Size: 30 3761
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 323 620 667 323
Size: 297 47 3447
Fragments arranged by size:
3447 297 47
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 399 805 1583 1773 2565 399
Size: 406 778 190 792 1625
Fragments arranged by size:
1625 792 778 406 190
BsmI GAATG_Cn'
Cuts at: 411 411
Size: 3791
Bsp1286I G_dGCh'C
Cuts at: 399 805 1416 1509 1583 1773 1835 2565 399
Size: 406 611 93 74 190 62 730 1625
Fragments arranged by size:
1625 730 611 406 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 313 841 1055 1357 1738 313
Size: 528 214 302 381 2366
Fragments arranged by size:
2366 528 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 235 539 1703 235
Size: 304 1164 2323
Fragments arranged by size:
2323 1164 304
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 451 2152 451
Size: 1701 2090
BstYI r'GATC_y
Cuts at: 1 816 1283 1306 1641 1887 2899 2985 2997
Size: 815 467 23 335 246 1012 86 12
Cuts at: 2997 3343 1
Size: 346 449
Fragments arranged by size:
1012 815 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1034
Size: 3791
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 188 357 740 1376 1550 1941 1968 3313 188
Size: 169 383 636 174 391 27 1345 666
Fragments arranged by size:
1345 666 636 391 383 174 169 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 161 341 1814 2024 161
Size: 180 1473 210 1928
Fragments arranged by size:
1928 1473 210 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 803 803
Size: 3791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 930 930
Size: 3791
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 576 1810 576
Size: 1234 2557
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 742 1552 742
Size: 810 2981
MslI CAynn'nnrTG
Cuts at: 1780 1907 1780
Size: 127 3664
MspA1I CmG'CkG
Cuts at: 155 256 902 1216 1576 2589 2834 155
Size: 101 646 314 360 1013 245 1112
Fragments arranged by size:
1112 1013 646 360 314 245 101
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 107 107
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 251 844 1344 2251 251
Size: 593 500 907 1791
Fragments arranged by size:
1791 907 593 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 256 1216 1576 256
Size: 960 360 2471
Fragments arranged by size:
2471 960 360
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 805 805
Size: 3791
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 150 527 834 1519 2512 2703 33
Size: 117 377 307 685 993 191 1121
Fragments arranged by size:
1121 993 685 377 307 191 117
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 398 766 946 1861 2353 2615 398
Size: 368 180 915 492 262 1574
Fragments arranged by size:
1574 915 492 368 262 180
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 251 844 1344 251
Size: 593 500 2698
Fragments arranged by size:
2698 593 500
StyI C'CwwG_G
Cuts at: 107 551 687 925 1279 107
Size: 444 136 238 354 2619
Fragments arranged by size:
2619 444 354 238 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 427 584 3306 427
Size: 157 2722 912
Fragments arranged by size:
2722 912 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 287 319 3308 3340 287
Size: 32 2989 32 738
Fragments arranged by size:
2989 738 32 32
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 750 1286 750
Size: 536 3255
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI