Restriction map for
pF1KB6921
(Circular) (Six-base) MAPSORT of: pF1KB6921.seq Check: 6231 from: 1 to: 3728
pF1KB6921 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 173 677 766 173
Size: 504 89 3135
Fragments arranged by size:
3135 504 89
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 182 1033 2600 182
Size: 851 1567 1310
Fragments arranged by size:
1567 1310 851
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 493 691 732 3081 3337 493
Size: 198 41 2349 256 884
Fragments arranged by size:
2349 884 256 198 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 651 753 651
Size: 102 3626
BanI G'GyrC_C
Cuts at: 670 744 1158 1406 1441 670
Size: 74 414 248 35 2957
Fragments arranged by size:
2957 414 248 74 35
BanII G_rGCy'C
Cuts at: 742 1772 742
Size: 1030 2698
BbeI G_GCGC'C
Cuts at: 674 1162 1410 674
Size: 488 248 2992
Fragments arranged by size:
2992 488 248
BbsI GAAGACnn'nnnn_
Cuts at: 258 258
Size: 3728
BciVI GTATCCnnnnn_n'
Cuts at: 251 1626 2387 3728 251
Size: 1375 761 1341 251
Fragments arranged by size:
1375 1341 761 251
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 599 2095 599
Size: 1496 2232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 546 632 341
Size: 205 86 3437
Fragments arranged by size:
3437 205 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 309 3432 309
Size: 3123 605
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 295 1242 3279 6
Size: 289 947 2037 455
Fragments arranged by size:
2037 947 455 289
BsaHI Gr'CG_yC
Cuts at: 164 671 1159 1407 2109 3605 164
Size: 507 488 248 702 1496 287
Fragments arranged by size:
1496 702 507 488 287 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 3728
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 742 1353 1446 1520 1710 1772 2502 742
Size: 611 93 74 190 62 730 1968
Fragments arranged by size:
1968 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 144 1726 1907 2116 144
Size: 1582 181 209 1756
Fragments arranged by size:
1756 1582 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 651 753 1220 1243 1578 1824 2836 2922
Size: 650 102 467 23 335 246 1012 86
Cuts at: 2922 2934 3280 1
Size: 12 346 449
Fragments arranged by size:
1012 650 467 449 346 335 246 102
86 23 12
BtgI C'CryG_G
Cuts at: 107 157 107
Size: 50 3678
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 169 1434 2292 169
Size: 1265 858 1605
Fragments arranged by size:
1605 1265 858
EaeI y'GGCC_r
Cuts at: 146 1313 1487 1878 1905 3250 146
Size: 1167 174 391 27 1345 624
Fragments arranged by size:
1345 1167 624 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 575 1936 2246 2392 3538 575
Size: 1361 310 146 1146 765
Fragments arranged by size:
1361 1146 765 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 546 632 1553 1985 2732 3000 341
Size: 205 86 921 432 747 268 1069
Fragments arranged by size:
1069 921 747 432 268 205 86
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1747 1747
Size: 3728
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 674 1162 1410 2432 3411 674
Size: 488 248 1022 979 991
Fragments arranged by size:
1022 991 979 488 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 1570 1600 1602 1632 320
Size: 32 1218 30 2 30 2416
Fragments arranged by size:
2416 1218 32 30 30 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 670 1158 1406 670
Size: 488 248 2992
Fragments arranged by size:
2992 488 248
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 646 1717 1844 646
Size: 1071 127 2530
Fragments arranged by size:
2530 1071 127
MspA1I CmG'CkG
Cuts at: 127 159 182 839 1153 1513 2526 2771 127
Size: 32 23 657 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 657 360 314 245 32 23
NaeI GCC'GGC
Cuts at: 146 1909 146
Size: 1763 1965
NarI GG'CG_CC
Cuts at: 671 1159 1407 671
Size: 488 248 2992
Fragments arranged by size:
2992 488 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3728
NgoMIV G'CCGG_C
Cuts at: 144 1907 144
Size: 1763 1965
NspI r_CATG'y
Cuts at: 781 1281 2188 781
Size: 500 907 2321
Fragments arranged by size:
2321 907 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PstI C_TGCA'G
Cuts at: 775 1460 775
Size: 685 3043
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 182 1153 1513 182
Size: 971 360 2397
Fragments arranged by size:
2397 971 360
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SacII CC_GC'GG
Cuts at: 160 160
Size: 3728
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 174 771 1456 2449 2640 33
Size: 141 597 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 191 141
SfoI GGC'GCC
Cuts at: 672 1160 1408 672
Size: 488 248 2992
Fragments arranged by size:
2992 488 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
SspI AAT'ATT
Cuts at: 201 201
Size: 3728
StyI C'CwwG_G
Cuts at: 107 656 862 1216 107
Size: 549 206 354 2619
Fragments arranged by size:
2619 549 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 491 557 1311 1444 1903 2112 491
Size: 66 754 133 459 209 2107
Fragments arranged by size:
2107 754 459 209 133 66
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 198 230 3245 3277 198
Size: 32 3015 32 649
Fragments arranged by size:
3015 649 32 32
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
XmnI GAAnn'nnTTC
Cuts at: 637 637
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI