Restriction map for
pF1KB6922
(Circular) (Six-base) MAPSORT of: pF1KB6922.seq Check: 7775 from: 1 to: 3794
pF1KB6922 3794 bp
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AccI GT'mk_AC
Cuts at: 832 832
Size: 3794
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1619 2051 2798 3066 423
Size: 1196 432 747 268 1151
Fragments arranged by size:
1196 1151 747 432 268
AfeI AGC'GCT
Cuts at: 757 757
Size: 3794
AflIII A'CryG_T
Cuts at: 1146 2250 3227 1146
Size: 1104 977 1713
Fragments arranged by size:
1713 1104 977
AgeI A'CCGG_T
Cuts at: 2182 2182
Size: 3794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 634 634
Size: 3794
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 190 222 190
Size: 32 3762
AlwNI CAG_nnn'CTG
Cuts at: 526 1099 2666 526
Size: 573 1567 1654
Fragments arranged by size:
1654 1567 573
ApaI G_GGCC'C
Cuts at: 185 185
Size: 3794
ApaLI G'TGCA_C
Cuts at: 2564 2564
Size: 3794
ApoI r'AATT_y
Cuts at: 798 3147 3403 798
Size: 2349 256 1189
Fragments arranged by size:
2349 1189 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 274 535 814 274
Size: 261 279 3254
Fragments arranged by size:
3254 279 261
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 780 810 1224 1472 1507 780
Size: 30 414 248 35 3067
Fragments arranged by size:
3067 414 248 35 30
BanII G_rGCy'C
Cuts at: 185 337 536 808 1838 185
Size: 152 199 272 1030 2141
Fragments arranged by size:
2141 1030 272 199 152
BbeI G_GCGC'C
Cuts at: 1228 1476 1228
Size: 248 3546
BciVI GTATCCnnnnn_n'
Cuts at: 1692 2453 3794 1692
Size: 761 1341 1692
Fragments arranged by size:
1692 1341 761
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglI GCCn_nnn'nGGC
Cuts at: 112 124 341 643 112
Size: 12 217 302 3263
Fragments arranged by size:
3263 302 217 12
BglII A'GATC_T
Cuts at: 1309 3346 1309
Size: 2037 1757
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 185 785 1419 1512 2568 185
Size: 600 634 93 1056 1411
Fragments arranged by size:
1411 1056 634 600 93
BmgBI CAC'GTC
Cuts at: 315 315
Size: 3794
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 3794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 475 622 349
Size: 126 147 3521
Fragments arranged by size:
3521 147 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 970 1849 2341 2639 736
Size: 234 879 492 298 1891
Fragments arranged by size:
1891 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 271 577 3498 271
Size: 306 2921 567
Fragments arranged by size:
2921 567 306
BsaAI yAC'GTr
Cuts at: 649 1147 649
Size: 498 3296
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 1225 1473 2175 3671 1225
Size: 248 702 1496 1348
Fragments arranged by size:
1496 1348 702 248
BsaWI w'CCGG_w
Cuts at: 983 1504 2182 2456 2603 3014 983
Size: 521 678 274 147 411 1763
Fragments arranged by size:
1763 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 553 517
Size: 36 3758
BseYI C'CCAG_C
Cuts at: 1219 1977 2554 3050 1219
Size: 758 577 496 1963
Fragments arranged by size:
1963 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 729
Size: 3794
BsiEI CG_ry'CG
Cuts at: 104 119 1336 1382 2590 104
Size: 15 1217 46 1208 1308
Fragments arranged by size:
1308 1217 1208 46 15
BsiHKAI G_wGCw'C
Cuts at: 808 1586 1776 2568 808
Size: 778 190 792 2034
Fragments arranged by size:
2034 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 185 337 536 785 808 1419 1512 1586 1776
Size: 152 199 249 23 611 93 74 190
Cuts at: 1776 1838 2568 185
Size: 62 730 1411
Fragments arranged by size:
1411 730 611 249 199 190 152 93
74 62 23
BspEI T'CCGG_A
Cuts at: 3014 3014
Size: 3794
BspHI T'CATG_A
Cuts at: 2970 2970
Size: 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 668 729 844 1058 1360 1741 668
Size: 61 115 214 302 381 2721
Fragments arranged by size:
2721 381 302 214 115 61
BsrBI CCG'CTC
Cuts at: 50 681 2086 2140 3624 3789 50
Size: 631 1405 54 1484 165 55
Fragments arranged by size:
1484 1405 631 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1706 1706
Size: 3794
BsrFI r'CCGG_y
Cuts at: 118 222 258 1792 1973 2182 118
Size: 104 36 1534 181 209 1730
Fragments arranged by size:
1730 1534 209 181 104 36
BssSI C'ACGA_G
Cuts at: 2065 2423 2065
Size: 358 3436
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 1171
Size: 3794
BstBI TT'CG_AA
Cuts at: 2155 2155
Size: 3794
BstYI r'GATC_y
Cuts at: 1 819 1286 1309 1644 1890 2902 2988 3000
Size: 818 467 23 335 246 1012 86 12
Cuts at: 3000 3346 1
Size: 346 449
Fragments arranged by size:
1012 818 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 338 338
Size: 3794
BtgI C'CryG_G
Cuts at: 785 785
Size: 3794
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1744 1925 2088 1744
Size: 181 163 3450
Fragments arranged by size:
3450 181 163
BtsI GCAGTG_nn'
Cuts at: 244 1037 244
Size: 793 3001
DraI TTT'AAA
Cuts at: 793 793
Size: 3794
DraIII CAC_nnn'GTG
Cuts at: 390 390
Size: 3794
DrdI GACnn_nn'nnGTC
Cuts at: 505 1500 2358 505
Size: 995 858 1941
Fragments arranged by size:
1941 995 858
EaeI y'GGCC_r
Cuts at: 116 260 1379 1553 1944 1971 3316 116
Size: 144 1119 174 391 27 1345 594
Fragments arranged by size:
1345 1119 594 391 174 144 27
EagI C'GGCC_G
Cuts at: 116 1379 116
Size: 1263 2531
EarI CTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2002 2312 2458 3604 2002
Size: 310 146 1146 2192
Fragments arranged by size:
2192 1146 310 146
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 423 475 622 1619 2051 2798 3066 349
Size: 74 52 147 997 432 747 268 1077
Fragments arranged by size:
1077 997 747 432 268 147 74 52
EcoNI CCTnn'n_nnAGG
Cuts at: 664 664
Size: 3794
EcoO109I rG'GnC_Cy
Cuts at: 181 182 933 181
Size: 1 751 3042
Fragments arranged by size:
3042 751 1
EcoRI G'AATT_C
Cuts at: 798 798
Size: 3794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 777 1813 441
Size: 336 1036 2422
Fragments arranged by size:
2422 1036 336
FspI TGC'GCA
Cuts at: 1575 1575
Size: 3794
HaeII r_GCGC'y
Cuts at: 759 777 1228 1476 2498 3477 759
Size: 18 451 248 1022 979 1076
Fragments arranged by size:
1076 1022 979 451 248 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1636 1666 1668 1698 1636
Size: 30 2 30 3732
Fragments arranged by size:
3732 30 30 2
HincII GTy'rAC
Cuts at: 687 705 833 687
Size: 18 128 3648
Fragments arranged by size:
3648 128 18
KasI G'GCGC_C
Cuts at: 1224 1472 1224
Size: 248 3546
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2649 2465
Size: 184 3610
MscI TGG'CCA
Cuts at: 1555 1555
Size: 3794
MslI CAynn'nnrTG
Cuts at: 616 1783 1910 616
Size: 1167 127 2500
Fragments arranged by size:
2500 1167 127
MspA1I CmG'CkG
Cuts at: 700 905 1219 1579 2592 2837 700
Size: 205 314 360 1013 245 1657
Fragments arranged by size:
1657 1013 360 314 245 205
NaeI GCC'GGC
Cuts at: 120 224 260 1975 120
Size: 104 36 1715 1939
Fragments arranged by size:
1939 1715 104 36
NarI GG'CG_CC
Cuts at: 1225 1473 1225
Size: 248 3546
NgoMIV G'CCGG_C
Cuts at: 118 222 258 1973 118
Size: 104 36 1715 1939
Fragments arranged by size:
1939 1715 104 36
NspI r_CATG'y
Cuts at: 847 1347 2254 847
Size: 500 907 2387
Fragments arranged by size:
2387 907 500
PciI A'CATG_T
Cuts at: 2250 2250
Size: 3794
PfoI T'CCnGG_A
Cuts at: 194 491 764 194
Size: 297 273 3224
Fragments arranged by size:
3224 297 273
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PmlI CAC'GTG
Cuts at: 649 649
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 196 228 2933 2965 196
Size: 32 2705 32 1025
Fragments arranged by size:
2705 1025 32 32
PspOMI G'GGCC_C
Cuts at: 181 181
Size: 3794
PstI C_TGCA'G
Cuts at: 740 841 1526 740
Size: 101 685 3008
Fragments arranged by size:
3008 685 101
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 700 1219 1579 700
Size: 519 360 2915
Fragments arranged by size:
2915 519 360
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SexAI A'CCwGG_T
Cuts at: 287 287
Size: 3794
SfcI C'TryA_G
Cuts at: 33 736 837 1522 2515 2706 33
Size: 703 101 685 993 191 1121
Fragments arranged by size:
1121 993 703 685 191 101
SfiI GGCCn_nnn'nGGCC
Cuts at: 124 124
Size: 3794
SfoI GGC'GCC
Cuts at: 1226 1474 1226
Size: 248 3546
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SmaI CCC'GGG
Cuts at: 816 816
Size: 3794
SmlI C'TyrA_G
Cuts at: 274 751 949 1864 2356 2618 274
Size: 477 198 915 492 262 1450
Fragments arranged by size:
1450 915 492 477 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 847 1347 847
Size: 500 3294
StyI C'CwwG_G
Cuts at: 928 1282 928
Size: 354 3440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 295 1377 1510 1969 2178 295
Size: 1082 133 459 209 1911
Fragments arranged by size:
1911 1082 459 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 3311 3343 299
Size: 32 2980 32 750
Fragments arranged by size:
2980 750 32 32
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 825 825
Size: 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1289 1289
Size: 3794
XhoI C'TCGA_G
Cuts at: 274 274
Size: 3794
XmaI C'CCGG_G
Cuts at: 814 814
Size: 3794
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI