Restriction map for
pF1KB6923
(Circular) (Six-base) MAPSORT of: pF1KB6923.seq Check: 7077 from: 1 to: 3728
pF1KB6923 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 766 766
Size: 3728
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 357 1033 2600 357
Size: 676 1567 1485
Fragments arranged by size:
1567 1485 676
ApaLI G'TGCA_C
Cuts at: 449 2498 449
Size: 2049 1679
ApoI r'AATT_y
Cuts at: 707 732 3081 3337 707
Size: 25 2349 256 1098
Fragments arranged by size:
2349 1098 256 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 3728
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 125 239 748 125
Size: 114 509 3105
Fragments arranged by size:
3105 509 114
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 744 1158 1406 1441 744
Size: 414 248 35 3031
Fragments arranged by size:
3031 414 248 35
BanII G_rGCy'C
Cuts at: 290 742 1772 290
Size: 452 1030 2246
Fragments arranged by size:
2246 1030 452
BbeI G_GCGC'C
Cuts at: 1162 1410 1162
Size: 248 3480
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 500 1248 500
Size: 748 2980
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 840 840
Size: 3728
Bme1580I G_kGCm'C
Cuts at: 453 1353 1446 2502 453
Size: 900 93 1056 1679
Fragments arranged by size:
1679 1056 900 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 2095 195
Size: 1900 1828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 904 1783 2275 2573 304
Size: 600 879 492 298 1459
Fragments arranged by size:
1459 879 600 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 1159 1407 2109 3605 1159
Size: 248 702 1496 1282
Fragments arranged by size:
1496 1282 702 248
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 265
Size: 3728
BsiEI CG_ry'CG
Cuts at: 104 1270 1316 2524 104
Size: 1166 46 1208 1308
Fragments arranged by size:
1308 1208 1166 46
BsiHKAI G_wGCw'C
Cuts at: 290 453 742 1520 1710 2502 290
Size: 163 289 778 190 792 1516
Fragments arranged by size:
1516 792 778 289 190 163
Bsp1286I G_dGCh'C
Cuts at: 290 453 742 1353 1446 1520 1710 1772 2502
Size: 163 289 611 93 74 190 62 730
Cuts at: 2502 290
Size: 1516
Fragments arranged by size:
1516 730 611 289 190 163 93 74
62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 1726 1907 2116 1726
Size: 181 209 3338
Fragments arranged by size:
3338 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 753 1220 1243 1578 1824 2836 2922 2934
Size: 752 467 23 335 246 1012 86 12
Cuts at: 2934 3280 1
Size: 346 449
Fragments arranged by size:
1012 752 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3728
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 971 971
Size: 3728
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 167 1313 1487 1878 1905 3250 167
Size: 1146 174 391 27 1345 645
Fragments arranged by size:
1345 1146 645 391 174 27
EagI C'GGCC_G
Cuts at: 1313 1313
Size: 3728
EarI CTCTTCn'nnn_
Cuts at: 605 1751 1961 605
Size: 1146 210 2372
Fragments arranged by size:
2372 1146 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 640 1936 2246 2392 3538 640
Size: 1296 310 146 1146 830
Fragments arranged by size:
1296 1146 830 310 146
EcoICRI GAG'CTC
Cuts at: 288 740 288
Size: 452 3276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1553 1985 2732 3000 1553
Size: 432 747 268 2281
Fragments arranged by size:
2281 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 393 867 393
Size: 474 3254
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 670 1747 670
Size: 1077 2651
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 115 158 1162 1410 2432 3411 115
Size: 43 1004 248 1022 979 432
Fragments arranged by size:
1022 1004 979 432 248 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1570 1600 1602 1632 1570
Size: 30 2 30 3666
Fragments arranged by size:
3666 30 30 2
HincII GTy'rAC
Cuts at: 767 767
Size: 3728
KasI G'GCGC_C
Cuts at: 1158 1406 1158
Size: 248 3480
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 2399 2583 521
Size: 1878 184 1666
Fragments arranged by size:
1878 1666 184
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 1717 1844 1717
Size: 127 3601
MspA1I CmG'CkG
Cuts at: 839 1153 1513 2526 2771 839
Size: 314 360 1013 245 1796
Fragments arranged by size:
1796 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 1159 1407 1159
Size: 248 3480
NcoI C'CATG_G
Cuts at: 107 107
Size: 3728
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 781 1281 2188 781
Size: 500 907 2321
Fragments arranged by size:
2321 907 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PfoI T'CCnGG_A
Cuts at: 395 479 395
Size: 84 3644
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 3696
PpuMI rG'GwC_Cy
Cuts at: 393 393
Size: 3728
PspXI vC'TCGA_Gb
Cuts at: 239 239
Size: 3728
PstI C_TGCA'G
Cuts at: 119 775 1460 119
Size: 656 685 2387
Fragments arranged by size:
2387 685 656
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 1153 1513 1153
Size: 360 3368
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 290 742 290
Size: 452 3276
SalI G'TCGA_C
Cuts at: 765 765
Size: 3728
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 115 771 1456 2449 2640 33
Size: 82 656 685 993 191 1121
Fragments arranged by size:
1121 993 685 656 191 82
SfoI GGC'GCC
Cuts at: 1160 1408 1160
Size: 248 3480
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 127 750 127
Size: 623 3105
SmlI C'TyrA_G
Cuts at: 239 283 883 1798 2290 2552 239
Size: 44 600 915 492 262 1415
Fragments arranged by size:
1415 915 600 492 262 44
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
SspI AAT'ATT
Cuts at: 378 378
Size: 3728
StyI C'CwwG_G
Cuts at: 107 862 1216 107
Size: 755 354 2619
Fragments arranged by size:
2619 755 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 468 1311 1444 1903 2112 468
Size: 843 133 459 209 2084
Fragments arranged by size:
2084 843 459 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1223 1223
Size: 3728
XhoI C'TCGA_G
Cuts at: 239 239
Size: 3728
XmaI C'CCGG_G
Cuts at: 125 748 125
Size: 623 3105
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI