Restriction map for
pF1KB6925
(Circular) (Six-base) MAPSORT of: pF1KB6925.seq Check: 2878 from: 1 to: 3728
pF1KB6925 3728 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 215 766 215
Size: 551 3177
Acc65I G'GTAC_C
Cuts at: 744 744
Size: 3728
AclI AA'CG_TT
Cuts at: 3568 3568
Size: 3728
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 449 521 1553 1985 2732 3000 404
Size: 45 72 1032 432 747 268 1132
Fragments arranged by size:
1132 1032 747 432 268 72 45
AflIII A'CryG_T
Cuts at: 1080 2184 3161 1080
Size: 1104 977 1647
Fragments arranged by size:
1647 1104 977
AgeI A'CCGG_T
Cuts at: 2116 2116
Size: 3728
AlwNI CAG_nnn'CTG
Cuts at: 1033 2600 1033
Size: 1567 2161
ApaLI G'TGCA_C
Cuts at: 2498 2498
Size: 3728
ApoI r'AATT_y
Cuts at: 732 3081 3337 732
Size: 2349 256 1123
Fragments arranged by size:
2349 1123 256
AseI AT'TA_AT
Cuts at: 20 586 20
Size: 566 3162
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
AvaI C'yCGr_G
Cuts at: 748 748
Size: 3728
BamHI G'GATC_C
Cuts at: 753 753
Size: 3728
BanI G'GyrC_C
Cuts at: 393 744 1158 1406 1441 393
Size: 351 414 248 35 2680
Fragments arranged by size:
2680 414 351 248 35
BanII G_rGCy'C
Cuts at: 742 1772 742
Size: 1030 2698
BbeI G_GCGC'C
Cuts at: 397 1162 1410 397
Size: 765 248 2715
Fragments arranged by size:
2715 765 248
BciVI GTATCCnnnnn_n'
Cuts at: 1626 2387 3728 1626
Size: 761 1341 1626
Fragments arranged by size:
1626 1341 761
BclI T'GATC_A
Cuts at: 1248 1248
Size: 3728
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 3719
BglII A'GATC_T
Cuts at: 1243 3280 1243
Size: 2037 1691
BlpI GC'TnA_GC
Cuts at: 226 840 226
Size: 614 3114
Bme1580I G_kGCm'C
Cuts at: 1353 1446 2502 1353
Size: 93 1056 2579
Fragments arranged by size:
2579 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 940 1021 1356 3500 940
Size: 81 335 2144 1168
Fragments arranged by size:
2144 1168 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 3728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 904 1783 2275 2573 904
Size: 879 492 298 2059
Fragments arranged by size:
2059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3432 3432
Size: 3728
BsaAI yAC'GTr
Cuts at: 1081 1081
Size: 3728
BsaBI GATnn'nnATC
Cuts at: 6 1242 3279 6
Size: 1236 2037 455
Fragments arranged by size:
2037 1236 455
BsaHI Gr'CG_yC
Cuts at: 187 394 1159 1407 2109 3605 187
Size: 207 765 248 702 1496 310
Fragments arranged by size:
1496 765 702 310 248 207
BsaWI w'CCGG_w
Cuts at: 917 1438 2116 2390 2537 2948 917
Size: 521 678 274 147 411 1697
Fragments arranged by size:
1697 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 460 430
Size: 30 3698
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 493 235
Size: 258 3470
BseYI C'CCAG_C
Cuts at: 1153 1911 2488 2984 1153
Size: 758 577 496 1897
Fragments arranged by size:
1897 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 194 1270 1316 2524 104
Size: 90 1076 46 1208 1308
Fragments arranged by size:
1308 1208 1076 90 46
BsiHKAI G_wGCw'C
Cuts at: 742 1520 1710 2502 742
Size: 778 190 792 1968
Fragments arranged by size:
1968 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 742 1353 1446 1520 1710 1772 2502 742
Size: 611 93 74 190 62 730 1968
Fragments arranged by size:
1968 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2948 2948
Size: 3728
BspHI T'CATG_A
Cuts at: 2904 2904
Size: 3728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 778 992 1294 1675 778
Size: 214 302 381 2831
Fragments arranged by size:
2831 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2020 2074 3558 3723 50
Size: 1970 54 1484 165 55
Fragments arranged by size:
1970 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1640 1640
Size: 3728
BsrFI r'CCGG_y
Cuts at: 131 1726 1907 2116 131
Size: 1595 181 209 1743
Fragments arranged by size:
1743 1595 209 181
BssSI C'ACGA_G
Cuts at: 1999 2357 1999
Size: 358 3370
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1105
Size: 3728
BstBI TT'CG_AA
Cuts at: 2089 2089
Size: 3728
BstYI r'GATC_y
Cuts at: 1 146 679 753 1220 1243 1578 1824 2836
Size: 145 533 74 467 23 335 246 1012
Cuts at: 2836 2922 2934 3280 1
Size: 86 12 346 449
Fragments arranged by size:
1012 533 467 449 346 335 246 145
86 74 23 12
BtgI C'CryG_G
Cuts at: 107 204 389 107
Size: 97 185 3446
Fragments arranged by size:
3446 185 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1678 1859 2022 1678
Size: 181 163 3384
Fragments arranged by size:
3384 181 163
BtsI GCAGTG_nn'
Cuts at: 477 971 477
Size: 494 3234
DraI TTT'AAA
Cuts at: 727 727
Size: 3728
DrdI GACnn_nn'nnGTC
Cuts at: 1434 2292 1434
Size: 858 2870
EaeI y'GGCC_r
Cuts at: 191 1313 1487 1878 1905 3250 191
Size: 1122 174 391 27 1345 669
Fragments arranged by size:
1345 1122 669 391 174 27
EagI C'GGCC_G
Cuts at: 191 1313 191
Size: 1122 2606
EarI CTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1936 2246 2392 3538 1936
Size: 310 146 1146 2126
Fragments arranged by size:
2126 1146 310 146
EcoICRI GAG'CTC
Cuts at: 740 740
Size: 3728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 449 521 1553 1985 2732 3000 404
Size: 45 72 1032 432 747 268 1132
Fragments arranged by size:
1132 1032 747 432 268 72 45
EcoO109I rG'GnC_Cy
Cuts at: 867 867
Size: 3728
EcoRI G'AATT_C
Cuts at: 732 732
Size: 3728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 165 344 1747 165
Size: 179 1403 2146
Fragments arranged by size:
2146 1403 179
FspI TGC'GCA
Cuts at: 1509 1509
Size: 3728
HaeII r_GCGC'y
Cuts at: 397 1162 1410 2432 3411 397
Size: 765 248 1022 979 714
Fragments arranged by size:
1022 979 765 714 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 551 583 1570 1600 1602 1632 261
Size: 32 258 32 987 30 2 30 2357
Fragments arranged by size:
2357 987 258 32 32 30 30 2
HincII GTy'rAC
Cuts at: 216 767 216
Size: 551 3177
HindIII A'AGCT_T
Cuts at: 541 541
Size: 3728
KasI G'GCGC_C
Cuts at: 393 1158 1406 393
Size: 765 248 2715
Fragments arranged by size:
2715 765 248
KpnI G_GTAC'C
Cuts at: 748 748
Size: 3728
MluI A'CGCG_T
Cuts at: 3161 3161
Size: 3728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2399 2583 2399
Size: 184 3544
MscI TGG'CCA
Cuts at: 1489 1489
Size: 3728
MslI CAynn'nnrTG
Cuts at: 551 608 1717 1844 551
Size: 57 1109 127 2435
Fragments arranged by size:
2435 1109 127 57
MspA1I CmG'CkG
Cuts at: 206 427 839 1153 1513 2526 2771 206
Size: 221 412 314 360 1013 245 1163
Fragments arranged by size:
1163 1013 412 360 314 245 221
NaeI GCC'GGC
Cuts at: 1909 1909
Size: 3728
NarI GG'CG_CC
Cuts at: 394 1159 1407 394
Size: 765 248 2715
Fragments arranged by size:
2715 765 248
NcoI C'CATG_G
Cuts at: 107 389 107
Size: 282 3446
NgoMIV G'CCGG_C
Cuts at: 1907 1907
Size: 3728
NspI r_CATG'y
Cuts at: 781 1281 2188 781
Size: 500 907 2321
Fragments arranged by size:
2321 907 500
PciI A'CATG_T
Cuts at: 2184 2184
Size: 3728
PmeI GTTT'AAAC
Cuts at: 727 727
Size: 3728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 148 180 2867 2899 148
Size: 32 2687 32 977
Fragments arranged by size:
2687 977 32 32
PstI C_TGCA'G
Cuts at: 426 642 775 1460 426
Size: 216 133 685 2694
Fragments arranged by size:
2694 685 216 133
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3728
PvuII CAG'CTG
Cuts at: 427 1153 1513 427
Size: 726 360 2642
Fragments arranged by size:
2642 726 360
RsrII CG'GwC_CG
Cuts at: 1923 1923
Size: 3728
SacI G_AGCT'C
Cuts at: 742 742
Size: 3728
SacII CC_GC'GG
Cuts at: 207 207
Size: 3728
SalI G'TCGA_C
Cuts at: 214 765 214
Size: 551 3177
SapI GCTCTTCn'nnn_
Cuts at: 1751 1961 1751
Size: 210 3518
SbfI CC_TGCA'GG
Cuts at: 775 775
Size: 3728
ScaI AGT'ACT
Cuts at: 3245 3245
Size: 3728
SfcI C'TryA_G
Cuts at: 33 363 422 638 771 1456 2449 2640 33
Size: 330 59 216 133 685 993 191 1121
Fragments arranged by size:
1121 993 685 330 216 191 133 59
SfiI GGCCn_nnn'nGGCC
Cuts at: 121 121
Size: 3728
SfoI GGC'GCC
Cuts at: 395 1160 1408 395
Size: 765 248 2715
Fragments arranged by size:
2715 765 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3728
SmaI CCC'GGG
Cuts at: 750 750
Size: 3728
SmlI C'TyrA_G
Cuts at: 883 1798 2290 2552 883
Size: 915 492 262 2059
Fragments arranged by size:
2059 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3728
SphI G_CATG'C
Cuts at: 781 1281 781
Size: 500 3228
StyI C'CwwG_G
Cuts at: 107 389 862 1216 107
Size: 282 473 354 2619
Fragments arranged by size:
2619 473 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1311 1444 1903 2112 1311
Size: 133 459 209 2927
Fragments arranged by size:
2927 459 209 133
TatI w'GTAC_w
Cuts at: 3243 3243
Size: 3728
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 3696
Tth111I GACn'n_nGTC
Cuts at: 1525 1525
Size: 3728
XbaI T'CTAG_A
Cuts at: 759 759
Size: 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1223 387
Size: 836 2892
XmaI C'CCGG_G
Cuts at: 748 748
Size: 3728
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI