Restriction map for
pF1KB6926
(Circular) (Six-base) MAPSORT of: pF1KB6926.seq Check: 9135 from: 1 to: 3797
pF1KB6926 3797 bp
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 1622 2054 2801 3069 306
Size: 1316 432 747 268 1034
Fragments arranged by size:
1316 1034 747 432 268
AfeI AGC'GCT
Cuts at: 769 769
Size: 3797
AflIII A'CryG_T
Cuts at: 250 1149 2253 3230 250
Size: 899 1104 977 817
Fragments arranged by size:
1104 977 899 817
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 235 235
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 183 491 779 1102 2669 183
Size: 308 288 323 1567 1311
Fragments arranged by size:
1567 1311 323 308 288
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 801 3150 3406 801
Size: 2349 256 1192
Fragments arranged by size:
2349 1192 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 822 822
Size: 3797
BanI G'GyrC_C
Cuts at: 813 1227 1475 1510 813
Size: 414 248 35 3100
Fragments arranged by size:
3100 414 248 35
BanII G_rGCy'C
Cuts at: 728 811 1841 728
Size: 83 1030 2684
Fragments arranged by size:
2684 1030 83
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BbsI GAAGACnn'nnnn_
Cuts at: 596 596
Size: 3797
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 533 1317 533
Size: 784 3013
BglI GCCn_nnn'nGGC
Cuts at: 676 676
Size: 3797
BglII A'GATC_T
Cuts at: 368 1312 3349 368
Size: 944 2037 816
Fragments arranged by size:
2037 944 816
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 200 1422 1515 2571 200
Size: 1222 93 1056 1426
Fragments arranged by size:
1426 1222 1056 93
BmgBI CAC'GTC
Cuts at: 528 528
Size: 3797
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 756 652
Size: 104 3693
Bpu10I CC'TnA_GC
Cuts at: 151 431 151
Size: 280 3517
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 592 628 3501 592
Size: 36 2873 888
Fragments arranged by size:
2873 888 36
BsaAI yAC'GTr
Cuts at: 1150 1150
Size: 3797
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 239 1228 1476 2178 3674 239
Size: 989 248 702 1496 362
Fragments arranged by size:
1496 989 702 362 248
BsaWI w'CCGG_w
Cuts at: 289 986 1507 2185 2459 2606 3017 289
Size: 697 521 678 274 147 411 1069
Fragments arranged by size:
1069 697 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 382 727 1222 1980 2557 3053 382
Size: 345 495 758 577 496 1126
Fragments arranged by size:
1126 758 577 496 495 345
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 811 1589 1779 2571 811
Size: 778 190 792 2037
Fragments arranged by size:
2037 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 200 728 811 1422 1515 1589 1779 1841 2571
Size: 528 83 611 93 74 190 62 730
Cuts at: 2571 200
Size: 1426
Fragments arranged by size:
1426 730 611 528 190 93 83 74
62
BspEI T'CCGG_A
Cuts at: 289 3017 289
Size: 2728 1069
BspHI T'CATG_A
Cuts at: 507 2973 507
Size: 2466 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 141 1795 1976 2185 141
Size: 1654 181 209 1753
Fragments arranged by size:
1753 1654 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 183 1174 183
Size: 991 2806
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstYI r'GATC_y
Cuts at: 1 368 737 822 1289 1312 1647 1893 2905
Size: 367 369 85 467 23 335 246 1012
Cuts at: 2905 2991 3003 3349 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 369 367 346 335 246
86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 201 201
Size: 3797
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 666 1747 1928 2091 666
Size: 1081 181 163 2372
Fragments arranged by size:
2372 1081 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1040
Size: 3797
DraI TTT'AAA
Cuts at: 796 796
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 238 1503 2361 238
Size: 1265 858 1674
Fragments arranged by size:
1674 1265 858
EaeI y'GGCC_r
Cuts at: 605 677 1382 1556 1947 1974 3319 605
Size: 72 705 174 391 27 1345 1083
Fragments arranged by size:
1345 1083 705 391 174 72 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 571 733 2005 2315 2461 3607 571
Size: 162 1272 310 146 1146 761
Fragments arranged by size:
1272 1146 761 310 162 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 306 652 756 1622 2054 2801 3069 306
Size: 346 104 866 432 747 268 1034
Fragments arranged by size:
1034 866 747 432 346 268 104
EcoNI CCTnn'n_nnAGG
Cuts at: 606 606
Size: 3797
EcoO109I rG'GnC_Cy
Cuts at: 278 488 936 278
Size: 210 448 3139
Fragments arranged by size:
3139 448 210
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1816 1816
Size: 3797
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 644 771 1231 1479 2501 3480 644
Size: 127 460 248 1022 979 961
Fragments arranged by size:
1022 979 961 460 248 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1669 1671 1701 1639
Size: 30 2 30 3735
Fragments arranged by size:
3735 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2468 2652 2468
Size: 184 3613
MscI TGG'CCA
Cuts at: 607 679 1558 607
Size: 72 879 2846
Fragments arranged by size:
2846 879 72
MslI CAynn'nnrTG
Cuts at: 512 658 1786 1913 512
Size: 146 1128 127 2396
Fragments arranged by size:
2396 1128 146 127
MspA1I CmG'CkG
Cuts at: 382 776 908 1222 1582 2595 2840 382
Size: 394 132 314 360 1013 245 1339
Fragments arranged by size:
1339 1013 394 360 314 245 132
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NspI r_CATG'y
Cuts at: 254 850 1350 2257 254
Size: 596 500 907 1794
Fragments arranged by size:
1794 907 596 500
PciI A'CATG_T
Cuts at: 250 2253 250
Size: 2003 1794
PfoI T'CCnGG_A
Cuts at: 629 629
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PstI C_TGCA'G
Cuts at: 368 844 1529 368
Size: 476 685 2636
Fragments arranged by size:
2636 685 476
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 776 1222 1582 776
Size: 446 360 2991
Fragments arranged by size:
2991 446 360
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 364 840 1525 2518 2709 33
Size: 331 476 685 993 191 1121
Fragments arranged by size:
1121 993 685 476 331 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 952 1867 2359 2621 952
Size: 915 492 262 2128
Fragments arranged by size:
2128 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StuI AGG'CCT
Cuts at: 436 436
Size: 3797
StyI C'CwwG_G
Cuts at: 602 686 931 1285 602
Size: 84 245 354 3114
Fragments arranged by size:
3114 354 245 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1380 1513 1972 2181 424
Size: 956 133 459 209 2040
Fragments arranged by size:
2040 956 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI